Pesquisa Agropecuaria Brasileira最新文献

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Antimicrobial activity of products based on potassium monopersulfate on bacteria associated with avian infections 过硫酸钾产品对禽类感染相关细菌的抑菌活性研究
4区 农林科学
Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03127
Miguel Augusto Moraes, Mariana Oliveira-Silva, Rafael Silva Goulart, Manoel Henrique Cintra Gabarra, Carlos Eduardo Saraiva Miranda, Paulo Garcia de Almeida, André Pitondo-Silva
{"title":"Antimicrobial activity of products based on potassium monopersulfate on bacteria associated with avian infections","authors":"Miguel Augusto Moraes, Mariana Oliveira-Silva, Rafael Silva Goulart, Manoel Henrique Cintra Gabarra, Carlos Eduardo Saraiva Miranda, Paulo Garcia de Almeida, André Pitondo-Silva","doi":"10.1590/s1678-3921.pab2023.v58.03127","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03127","url":null,"abstract":"Abstract The objective of this work was to evaluate the antimicrobial activity of four new potassium monopersulfate-based products on bacterial biofilms associated with avian infections, in order to disinfect drinking fountains in poultry farms. Initially, tests were performed in planktonic bacterial cells, to verify the antimicrobial activity and the minimum inhibitory concentration of the products, named PA, PB, PC, and PD. These products were tested on mature biofilms of the avian pathogens Escherichia coli, Salmonella enterica subsp. enterica serovar Enteritidis, and Listeria monocytogenes, grown on specimens preserved in acrylic blocks, by counting colony-forming units, scanning electron microscopy, and confocal fluorescence microscopy. All products were effective against the evaluated bacterial species. The PA and PB products inhibited the bacterial growth at ≤ 0.13% concentrations, and PC and PD showed the same effect at ≤ 0.25% concentrations. Furthermore, the PA product was able to eliminate mature biofilms of S. enterica subsp. enterica serovar Enteritidis and L. monocytogenes. The evaluated monopersulfate-based products, notably PA, are effective against bacterial biofilms associated with avian infections and show potential as sanitizers and disinfectants for drinking fountains in poultry farms.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136305570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
2NVS translocation presence and inheritance of resistance to head blast in wheat 小麦穗瘟病抗性的nvs易位存在与遗传
4区 农林科学
Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03038
Camila Vancini, Gisele Abigail Montan Torres, Luciano Consoli, João Leodato Nunes Maciel, Ricardo Lima de Castro, Natália Forchezato Webber, Diógenes Cecchin Silveira, Carolina Cardoso Deuner
{"title":"2NVS translocation presence and inheritance of resistance to head blast in wheat","authors":"Camila Vancini, Gisele Abigail Montan Torres, Luciano Consoli, João Leodato Nunes Maciel, Ricardo Lima de Castro, Natália Forchezato Webber, Diógenes Cecchin Silveira, Carolina Cardoso Deuner","doi":"10.1590/s1678-3921.pab2023.v58.03038","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03038","url":null,"abstract":"Abstract The objective of this work was to evaluate the effect of the presence of 2NVS translocation on the inheritance of wheat resistance to head blast, in F2 segregating lines from two biparental crosses. The IPF 86766 and 'Santa Fe' genotypes were used as female parents (both 2NVS carriers), and 'BRS 404', as the common male parent (non 2NVS carrier). Using a mixture of three isolates of Pyricularia oryzae Triticum, wheat spikes and rachises were evaluated for bleaching severity at five (%BS_5dpi) and seven days post-inoculation (%BS_7dpi), as well as for the number of infection points (IPR) and pathogen sporulation (SPO). Chi-square analyses showed that segregation follows a 3:1 Mendelian hypothesis, with a major locus with a dominant allele: %BS_7dpi for IPF 86766 × 'BRS 404' and IPR for 'Santa Fe' x 'BRS 404'. Therefore, this is the first known study to show that the 2NVS translocation responsible for resistance to head blast is a major locus with a dominant allele. In a controlled environment, 'BRS 404' shows bleaching severity and SPO similar with those occurring on IPF 86766 and 'Santa Fe'.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136303029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modeling the yield of winter maize using biomass distribution index in the tropical region of Yunnan, China 利用生物量分布指数模拟云南热带地区冬玉米产量
4区 农林科学
Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03221
Xinyuan Han, Chen Yang, Xiao Weihua, Jie Zhou, Wenfeng Li
{"title":"Modeling the yield of winter maize using biomass distribution index in the tropical region of Yunnan, China","authors":"Xinyuan Han, Chen Yang, Xiao Weihua, Jie Zhou, Wenfeng Li","doi":"10.1590/s1678-3921.pab2023.v58.03221","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03221","url":null,"abstract":"Abstract The objective of this work was to establish and validate the dry matter distribution and yield prediction models based on physiological developmental timing, to compare the differences between the dry mass distribution index model and the dry mass distribution coefficient model, for the simulation of ear dry mass and to improve the accuracy of maize growth models for predicting yield. The experiments were conducted in three tropical sites (Longchuan, Mangshi, and Ruili) in the tropical region of Yunnan Province, China. The NRMS of ear dry mass and yield were generally less than 10. The dry mass distribution index method (NRMS = 5.44% and RMSE = 807.22 kg ha-1 for ear dry mass; and NRMS = 7.32% and RMSE = 707.67 kg ha-1 for grain yield) is better than the dry mass distribution coefficient method (NRMS = 7.52% and RMSE = 1115.31 kg ha-1 for ear dry mass; NRMS = 8.6% and RMSE = 830.76 kgha-1 for grain yield) to simulate maize ear dry mass and grain yield. The distribution index model improves the accuracy of the model, which is valuable for future maize production and management in Yunnan.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135261477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lipid profile of artisanal Minas cheese from certified regions in the state of Minas Gerais, Brazil 来自巴西米纳斯吉拉斯州认证地区的手工米纳斯吉拉斯州奶酪的脂质特征
4区 农林科学
Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03212
Adélia Ferreira Dargère, Sandra Maria Pinto, Jonas Guimarães e Silva, Cesar Augusto Pospissil Garbossa, Diogo Santos Batista, Laryssa Fernandes Correia, Joanna Oliveira Marçal, Peter Bitencourt Faria
{"title":"Lipid profile of artisanal Minas cheese from certified regions in the state of Minas Gerais, Brazil","authors":"Adélia Ferreira Dargère, Sandra Maria Pinto, Jonas Guimarães e Silva, Cesar Augusto Pospissil Garbossa, Diogo Santos Batista, Laryssa Fernandes Correia, Joanna Oliveira Marçal, Peter Bitencourt Faria","doi":"10.1590/s1678-3921.pab2023.v58.03212","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03212","url":null,"abstract":"Abstract The objective of this work was to determine the fatty acid profile of artisanal Minas cheese from seven certified regions in the state of Minas Gerais, Brazil. A total of 78 samples were collected in regions where the producers were registered by Instituto Mineiro de Agropecuária. For lipid profile determination, different cheese varieties from the following regions were analyzed: Canastra, Serro, Araxá, Serra do Salitre, Triângulo Mineiro, Campo das Vertentes, and Cerrado. The lipid profile of Serra do Salitre and Araxá cheeses was similar in caproic acid (C6:0), total polyunsaturated fatty acids, and ratio of polyunsaturated and saturated fatty acids. In the other regions, lipid profile differed due to associations with specific fatty acids, such as those of the cheeses from Triângulo Mineiro with C20:5n3, of Cerrado with C20:4n6, of Canastra with C8:0, of Serro with C22:2 and C22:6n3, and of Campo das Vertentes with C17:0, C18:0, and C20:0. The fatty acid profile and nutritional indices related to the lipid profile of artisanal Minas cheeses differ according to the region of origin. The content and profile of omega 3 fatty acids are significant parameters to differentiate artisanal Minas cheeses. Serro artisanal Minas cheese presents superior nutritional indices concerning fatty acid profile than the other studied cheeses.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135262468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interrelationship between potassium rates and the efficiency of Bt genes in the control of Spodoptera frugiperda 施钾率与Bt基因防治夜蛾效率的相互关系
4区 农林科学
Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03241
Marcelo Macedo Faria, Pedro Henrique Nascimento Cintra, Víctor Alves Amorim, Thiago Souza Campos, Ednaldo Cândido Rocha, Fabricio Rodrigues
{"title":"Interrelationship between potassium rates and the efficiency of Bt genes in the control of Spodoptera frugiperda","authors":"Marcelo Macedo Faria, Pedro Henrique Nascimento Cintra, Víctor Alves Amorim, Thiago Souza Campos, Ednaldo Cândido Rocha, Fabricio Rodrigues","doi":"10.1590/s1678-3921.pab2023.v58.03241","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03241","url":null,"abstract":"Abstract The objective of this work was to identify the effect of potassium rates on Bt transgenic resistance and damage caused by Spodoptera frugiperda in corn plants at different developmental stages. The experimental design was a randomized complete block in a 3x6 factorial arrangement, with three potassium rates (0, 45, and 90 kg ha-1) and six corn hybrids (20A55Hx, 30A77PW, AG1051, MG652PW, NS90VTPro2, and NS92VTPro), with three replicates. The hybrids were evaluated through the analysis of canonical variables. With low potassium rates, the effectiveness of the corn hybrids in controlling the S. frugiperda caterpillar decreases in the different phenological stages. The use of the Bt technology is more efficient under an adequate potassium rate of 90 kg ha-1 KCl.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135059732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Temporal dynamics of Plasmopara viticola as function of bud load increase in 'Sauvignon Blanc' 白苏维浓(Sauvignon Blanc)芽量增加对葡萄浆原菌时间动态的影响
4区 农林科学
Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03224
Douglas André Wurz, Alberto Fontanella Brighenti, Ricardo Allebrandt, Leo Rufato
{"title":"Temporal dynamics of Plasmopara viticola as function of bud load increase in 'Sauvignon Blanc'","authors":"Douglas André Wurz, Alberto Fontanella Brighenti, Ricardo Allebrandt, Leo Rufato","doi":"10.1590/s1678-3921.pab2023.v58.03224","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03224","url":null,"abstract":"Abstract The objective of this work was to evaluate the effect of increasing bud load per plant on epidemiological variables of downy mildew on 'Sauvignon Blanc'. The study was carried out in a commercial vineyard in a high-altitude region of the municipality of São Joaquim, in the state of Santa Catarina, Brazil, during the 2016/2017 and 2017/2018 vintages. The treatments consisted of four bud loads: 15, 30, 50, and 75 per vine. The maximum incidence, maximum severity, beginning of symptom appearance, time to reach maximum disease incidence and severity, area under the incidence and severity disease progress curve were determined. Increasing bud load over 50 per vine results in higher incidence and severity of downy mildew (Plasmopara viticola) in 'Sauvignon Blanc' leaves but does not influence the time to reach maximum disease incidence and severity. Increasing bud load over 50 per vine results in an increase in the area under the disease incidence and severity progress curve for downy mildew in 'Sauvignon Blanc' leaves. To adopt over 50 buds per vine treatment, it is necessary additional disease control measures, with an emphasis on integrated management to control downy mildew.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135059744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Growth promotion in different Urochloa species inoculated with Azospirillum baldaniorum 接种氮螺旋菌对不同种类尿藻生长的促进作用
4区 农林科学
Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03079
Liliandra Barreto Emídio Gomes, Thamires Ferreira Rodrigues da Silva, Gabriela Cavalcanti Alves, Veronica Massena Reis
{"title":"Growth promotion in different Urochloa species inoculated with Azospirillum baldaniorum","authors":"Liliandra Barreto Emídio Gomes, Thamires Ferreira Rodrigues da Silva, Gabriela Cavalcanti Alves, Veronica Massena Reis","doi":"10.1590/s1678-3921.pab2023.v58.03079","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03079","url":null,"abstract":"Abstract The objective of this work was to evaluate inoculation response to Azospirillum baldaniorum strain Sp245 in 14 Urochloa cultivars. The evaluated cultivars were: Marandú, Xaraés, Paiaguás, Piatã, Basilisk, B140, Comum, Tupi, H47, Llanero, Ruziziensis, Ipyporã, Mulato, and Convert HD364. Three experiments were conducted to measure root traits: two using soil and one a sterile substrate. Compared with the control, a single application of A. baldaniorum Sp245 increases dry matter accumulation and number of roots in cultivars Marandú, Llanero, Ruziziensis, and Convert HD364. However, after inoculation, cultivars Xaraés and Ipyporã show some root traits with lower values.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135700374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic parameters and validation of microsatellite markers associated with iron and zinc in common bean 蚕豆铁、锌相关微卫星标记的遗传参数及验证
4区 农林科学
Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03267
Poliana Regina Carloni, Thiago Lívio Pessoa Oliveira de Souza, Marcelo Sfeir de Aguiar, Leonardo Cunha Melo, Patrícia Guimarães Santos Melo, Helton Santos Pereira
{"title":"Genetic parameters and validation of microsatellite markers associated with iron and zinc in common bean","authors":"Poliana Regina Carloni, Thiago Lívio Pessoa Oliveira de Souza, Marcelo Sfeir de Aguiar, Leonardo Cunha Melo, Patrícia Guimarães Santos Melo, Helton Santos Pereira","doi":"10.1590/s1678-3921.pab2023.v58.03267","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03267","url":null,"abstract":"Abstract The objective of this work was to estimate the genetic parameters, evaluate the agronomic performance, and validate the microsatellite molecular markers (SSRs) linked with quantitative trait loci (QTLs) for Fe and Zn concentrations in grains of common bean, in order to select superior lines. One hundred and sixteen lines from two populations ('BRS Requinte' × 'Porto Real' and 'BRS Requinte' × G2358) and five check genotypes were evaluated in three environments. The parents and lines were genotyped with 20 SSRs. In the simultaneous selection of the lines for the four evaluated traits, the gains from selection were 4.7% for Fe concentration, 2.8% for Zn concentration, 3.9% for yield, and 0.9% for 100-seed weight. Therefore, there is the possibility of selection of lines that combine desirable phenotypes for the traits of interest. The only polymorphic marker is BM 154 in the 'BRS Requinte' × 'Porto Real' population, indicating that the QTLs linked with the markers may already be fixed or that the markers are not associated in the used populations. The single-marker analysis of QTL mapping shows an association between BM 154 and Fe concentration in only one environment, explaining 14.5% of phenotypic variation, which indicates the occurrence of the interaction of QTLs with environments.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135261464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cover plants with potential to reduce two-spotted spider mite population in soybean 覆盖有潜力减少大豆双斑蜘蛛螨种群的植物
4区 农林科学
Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03332
Luana Cristina Alves Ribeiro, Felipe Lemos, Dalila Dominique Duarte Rocha, Marcos Antônio Matiello Fadini
{"title":"Cover plants with potential to reduce two-spotted spider mite population in soybean","authors":"Luana Cristina Alves Ribeiro, Felipe Lemos, Dalila Dominique Duarte Rocha, Marcos Antônio Matiello Fadini","doi":"10.1590/s1678-3921.pab2023.v58.03332","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03332","url":null,"abstract":"Abstract The objective of this work was to evaluate the potential of cover plants in reducing the population of the two-spotted spider mite, Te t r a n y c h u s urticae, in soybean. Seven host plants – cotton (Gossypium hirsutum), Urochloa ruziziensis, crotalaria (Crotalaria juncea), corn (Zea mays), radish (Raphanus sativus), soybean (Glycine max), and sorghum (Sorghum bicolor) – were evaluated for their effect on T. urticae biological parameters (oviposition, development stages, and survival) and preference for volatile odors (using a Y-tube olfactometer). Tetranychus urticae preferred cotton and showed a higher population growth rate on this host plant. The survival and oviposition of T. urticae was prolonged in radish and crotalaria plants, whereas juveniles did not complete their immature development on U. ruziziensis leaves. No preference was observed between the volatile odors of U. ruziziensis, radish, and crotalaria. Urochloa ruziziensis can reduce T. urticae populations in soybean plants.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135262465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic diversity of Xanthomonas phaseoli p v. manihotis populations using rep-PCR and VNTR molecular markers 利用rep-PCR和VNTR分子标记分析相黄单胞菌的遗传多样性
4区 农林科学
Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03299
Laís Barreto de Oliveira, Saulo Alves Santos de Oliveira, Ricardo Franco Cunha Moreira, Maria Selma Alves Silva Diamantino, Andresa Priscila de Souza Ramos, Taliane Leila Soares, Claudia Fortes Ferreira
{"title":"Genetic diversity of Xanthomonas phaseoli p v. manihotis populations using rep-PCR and VNTR molecular markers","authors":"Laís Barreto de Oliveira, Saulo Alves Santos de Oliveira, Ricardo Franco Cunha Moreira, Maria Selma Alves Silva Diamantino, Andresa Priscila de Souza Ramos, Taliane Leila Soares, Claudia Fortes Ferreira","doi":"10.1590/s1678-3921.pab2023.v58.03299","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03299","url":null,"abstract":"Abstract The objective of this work was to evaluate the genetic diversity of Xanthomonas phaseoli p v. manihotis (Xpm) from eight populations from five cassava producing states in Brazil, through the rep-PCR (BOX-PCR and ERIC-PCR) and variable number of tandem repeat (VNTR) markers. Cassava leaves with symptoms of cassava bacterial blight were collected in eight municipalities, and the Xpm isolates were identified by amplification with primers specific for these isolates. The identity of the Xpm isolates was confirmed with the BOX-PCR, ERIC-PCR, and VNTR markers. The observed selection pressure, together with the mode of reproduction and the mechanisms that increase genetic variability, allows of the pathogen populations to adapt according to microclimate variation, contributing to a differentiated reproductive success. ERIC-PCR and VNTRs are the best markers for evaluating the genetic variability in the eight studied Xpm populations. However, ERIC-PCR is the marker that best separated the groups by population and presented a higher similarity between the isolates of the same population. The study of the genetic diversity of Xpm is key to improve disease monitoring and management strategies in cassava crops.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135260819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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