European Journal of Dental and Oral Health最新文献

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Dens Invaginatus: Literature Review and Case Report 牙槽内陷:文献回顾及病例报告
European Journal of Dental and Oral Health Pub Date : 2023-08-31 DOI: 10.24018/ejdent.2023.4.4.275
Wissal Kabbassi, Jalila Hammouti, Hind Ramdi
{"title":"Dens Invaginatus: Literature Review and Case Report","authors":"Wissal Kabbassi, Jalila Hammouti, Hind Ramdi","doi":"10.24018/ejdent.2023.4.4.275","DOIUrl":"https://doi.org/10.24018/ejdent.2023.4.4.275","url":null,"abstract":"Dens invaginatus (DI) represents a structural defect in the tooth, resulting from invagination of enamel organ into the dental papilla. This rare anomaly, which often goes unnoticed during a clinical examination, is a risk factor for the development of carious lesions and consequently pulpopathies. The knowledge of this type of lesions allows to ensure early diagnosis and thus avoid any complications. This article represents a case report of dens invaginatus along with a literature review regarding etiology, classification, clinical appearance, and diagnosis. It provides also guidelines for decision-making and treatment of invaginated teeth","PeriodicalId":197045,"journal":{"name":"European Journal of Dental and Oral Health","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135988533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Surgical Root Coverage of Miller’s Class III Using Free Gingival Autograft (A Case Report) 自体游离牙龈移植修复Miller氏ⅲ类牙根(附1例报告)
European Journal of Dental and Oral Health Pub Date : 2023-08-31 DOI: 10.24018/ejdent.2023.4.4.253
Mulyati Mulyati, K. Agung, P. Yunita
{"title":"Surgical Root Coverage of Miller’s Class III Using Free Gingival Autograft (A Case Report)","authors":"Mulyati Mulyati, K. Agung, P. Yunita","doi":"10.24018/ejdent.2023.4.4.253","DOIUrl":"https://doi.org/10.24018/ejdent.2023.4.4.253","url":null,"abstract":"Background: Gingival recession is a condition where the root surface of the tooth is exposed due to migration of the gingival margin and junctional epithelium in an apical direction. The problem that patients often complain about due to gingival recession is an aesthetic problem, especially if the recession occurs in the upper anterior teeth. In addition, gingival recession can also cause dentine hypersensitivity due to the exposure of the root surface that was previously covered by the gingiva. One of the treatment options for patients with gingival recession is free gingival autograft surgery. Purpose: The goal of this article is to explain how to manage gingival recession therapy with free gingival autograft surgery in patients with Miller Class III recession. Case: A 29-year-old female patient came to RSGM Airlangga University with complaints of hypersensitivity in the front teeth at the lower jaw. The last scaling took place about 6 months ago. The patient does not have a history of systemic diseases, such as hypertension and diabetes, and the patient does not have allergies to either drugs or food. Case Management: Based on the examination that has been carried out, the treatment option for Miller's class III gingival recession in the tooth region 41 is agreed to be a free gingival autograft. Conclusion: One of the root coverage treatment options is free gingival autograft, where this procedure can also increase the width of the attached gingiva.","PeriodicalId":197045,"journal":{"name":"European Journal of Dental and Oral Health","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135988532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Managing Maxillofacial Defects with Hollow Closed Bulb Obturator Prosthesis 空心闭孔修复颌面部缺损
European Journal of Dental and Oral Health Pub Date : 2023-08-31 DOI: 10.24018/ejdent.2023.4.4.286
Sanaa Rimaoui, Soukaina Oujdad, Samira Bellemkhannate
{"title":"Managing Maxillofacial Defects with Hollow Closed Bulb Obturator Prosthesis","authors":"Sanaa Rimaoui, Soukaina Oujdad, Samira Bellemkhannate","doi":"10.24018/ejdent.2023.4.4.286","DOIUrl":"https://doi.org/10.24018/ejdent.2023.4.4.286","url":null,"abstract":"Maxillary resection surgery in response to tumour processes often results in loss of substance, leading to bucco-nasal or bucco-naso-sinus communication. The resulting consequences are significant, including difficulties with eating, with food and fluids leaking out, phonetic problems resulting in a nasal voice and incomprehensible speech. These problems are compounded by aesthetic problems such as sagging skin, asymmetry and facial disfigurement, particularly in cases where the facial tissues are affected. This article describes the case of a patient with maxillary bone loss associated with a deficit of jugal covering tissues. We decided to fabricate a cast metal partial denture with a hollow obturator, and we will detail the steps involved in its fabrication and describe the impression techniques used. Given the absence of a mandibular prosthetic corridor, we will also describe our approach to rehabilitating the mandibular arch to prevent food and fluid spillage through the jugal gap. In this context, our main objective was to ensure a watertight seal to guarantee optimal function.","PeriodicalId":197045,"journal":{"name":"European Journal of Dental and Oral Health","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136036089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence of Dental Caries among Adult Patients in Bungoma County, Kenya 肯尼亚本戈马县成年患者龋齿患病率
European Journal of Dental and Oral Health Pub Date : 2023-08-24 DOI: 10.24018/ejdent.2023.4.4.273
Caren M. Sumba, D. Kokonya, B. Wesonga
{"title":"Prevalence of Dental Caries among Adult Patients in Bungoma County, Kenya","authors":"Caren M. Sumba, D. Kokonya, B. Wesonga","doi":"10.24018/ejdent.2023.4.4.273","DOIUrl":"https://doi.org/10.24018/ejdent.2023.4.4.273","url":null,"abstract":"\u0000\u0000\u0000\u0000Globally, dental caries has affected nearly 100% of adult teeth.  Dental caries is caused when microbial biofilm converts free sugar contained in such foods and drinks into acid which corrodes the tooth structure. In Kenya, the prevalence is 34%. This high prevalence has led to the negative impact associated with the disease. Therefore, this study’s main aim was to determine the prevalence of dental caries among adult patients in Bungoma County, Kenya. This study was conducted in Bungoma County and adopted a cross-sectional design. The target population was adult dental patients and the key informants were the dentist and dental technologist. Participants were selected using multistage sampling techniques. The sample size of 347 dental patients was arrived at using Cochran’s formula. Data were collected using the WHO-modified assessment questionnaire. WHO DMFT index checklist was also used. Data were analysed using descriptive and inferential statistics with the aid of the Statistical Package for Social Sciences 25.0. Data were presented using tables and figures. Logistic regression was used to determine the direction of the association at the significance level set at 0.05. Thematic analysis was used to analyse qualitative data. The researcher observed the ethical principles applicable to the study.  The prevalence of dental caries among adult patients was 43.7% with a mean total DMFT index of 3.249 which signifies a moderate distribution among the adult population. Kimilili sub-county hospital had a moderate mean DMFT index of 4.47 and Webuye County hospital had a low mean DMFT index of 1.585. Dental caries was more prevalent in youths aged between 18 years to 35 years (60.1%). Moreover, the female gender has more dental caries prevalence at 57.4% as compared to male patients. The prevalence of dental caries is more prone among the rural (56.8%) as compared to the urban (43.2%). According to incomes, individuals who earn more than Ksh. 23,750 have a higher prevalence of 65.6%) than those who earn less than Ksh. 23,750 (34.4%). Dental caries is more prominent in the lower jaw. Those who do not brush their teeth were 4 times more likely to develop dental caries as compared to those who practise good oral hygiene practices (OR =3.0). Those who consume sugary foods and drinks frequently (OR: 2.4) and those who smoke tobacco (OR: 2.0) were times more likely to develop dental caries compared to those who did not consume sugary foods and smoke tobacco. Conclusion: The prevalence of dental caries among adults is moderate with the prevalence being higher in youths, female, low-income earners (< ksh.23,750) and rural dwellers. In terms of the jaws, the lower jaw is more susceptible to caries attack compared to the upper jaw and also the posterior teeth (premolar and molars) are more susceptible to caries compared to the upper jaw. Recommendation: There is a need to conduct a community-based campaign even through Chiefs’ Barazas on the importance of p","PeriodicalId":197045,"journal":{"name":"European Journal of Dental and Oral Health","volume":"251 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132116391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rate of Enterococcus Faecalis in Saliva and Failed Root Canal Treated Teeth—In Vivo Study 唾液中粪肠球菌率与根管治疗失败牙的体内研究
European Journal of Dental and Oral Health Pub Date : 2023-08-23 DOI: 10.24018/ejdent.2023.4.4.288
Tahmida Hoque, Mozammal Hossain, Sharmin Mahmud, Ahmed Abu Saleh, Mohammad Ali Asgor Moral
{"title":"Rate of Enterococcus Faecalis in Saliva and Failed Root Canal Treated Teeth—In Vivo Study","authors":"Tahmida Hoque, Mozammal Hossain, Sharmin Mahmud, Ahmed Abu Saleh, Mohammad Ali Asgor Moral","doi":"10.24018/ejdent.2023.4.4.288","DOIUrl":"https://doi.org/10.24018/ejdent.2023.4.4.288","url":null,"abstract":"Various bacteria were discovered in the root canal system that had been treated, where Enterococci were prevalent and heavily to blame for the failure. For the purpose of achieving clinical success, research is required to determine the prevalence of Enterococcus faecalis in the space between saliva and a root canal. This observational cross-sectional analytical study’s objective was to examine the prevalence of E. faecalis in saliva and in the root canals of teeth that required retreatment after prior endodontic treatment. The patient was chosen for re-RCT from the OPD of the BSMMU Department of Conservative Dentistry and Endodontics. First, a sample of saliva was taken. The carious lesion and coronal restoration were inactivated, the damaged tooth was isolated, and it was decontaminated.\u0000Without using a chemical solvent, the root canal filling was removed. A radiograph was used to measure and confirm the canal’s length. The microbiology lab received the paper points and saliva samples for culture (Enterococcus faecalis identification). According to the findings, saliva samples contained 11 (25%) and root canal samples had 27 (61.4%) instances of Enterococcus faecalis. There was a significant difference between the two samples (p 0.05).\u0000In patients who needed a repeat RCT, Enterococcus faecalis is far more common in the root canal than in the patient’s saliva.","PeriodicalId":197045,"journal":{"name":"European Journal of Dental and Oral Health","volume":"131 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115501289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the Effectiveness of Tooth Whitening of Using Reservoir in Bleaching Trays as Compared to Using Bleaching Tray without the Reservoir: An Observational Clinical Trial 与不使用贮槽的漂白托盘相比,使用贮槽的漂白托盘牙齿美白效果的评价:一项观察性临床试验
European Journal of Dental and Oral Health Pub Date : 2023-07-28 DOI: 10.24018/ejdent.2023.4.4.280
T. Rabi, Mira Salloum
{"title":"Evaluation of the Effectiveness of Tooth Whitening of Using Reservoir in Bleaching Trays as Compared to Using Bleaching Tray without the Reservoir: An Observational Clinical Trial","authors":"T. Rabi, Mira Salloum","doi":"10.24018/ejdent.2023.4.4.280","DOIUrl":"https://doi.org/10.24018/ejdent.2023.4.4.280","url":null,"abstract":"Background: Bleaching procedures have gained importance due to the increase in aesthetic demand. At-home bleaching procedures have become popular due to their ease of application at their own time. At-home bleaching procedures can be modified to increase effectiveness.\u0000Aim: The present study aims to compare the effectiveness of tooth whitening by using bleaching trays with reservoirs and ones without reservoirs.\u0000Materials and Methods: The present study is an observational clinical trial done on 24 patients in Palestine. All the caries-free participants with Canine shades darker than A2 were included in the present study. The sample was divided into two groups. One group received bleaching trays with a reservoir, another group received bleaching trays without a reservoir. 10% carbamide peroxide was used as a bleaching agent in both groups for 21 days with 3-hour wear of trays. The primary outcome measured in the study was color change using the spectrophotometer VITA Easyshade. The secondary outcomes measured in the study were post-bleaching gingival irritation and tooth sensitivity. Statistical analysis was done using SPSS software. T-test was done to compare the primary outcomes between the two groups and the Chi-square test was done to compare the secondary outcomes.\u0000Results: T-test showed that there is no significant difference between the color change in the two groups. The chi-square test showed that there is no significant difference in the occurrence of gingival irritation and tooth sensitivity between the two groups (P>0.05).\u0000Conclusion: There is no significant difference in color change between the bleaching tray with the reservoir and without the reservoir. There is no significant difference in the increase in gingival irritation or tooth sensitivity between the two groups. Further studies and meta-analyses need to be conducted to obtain more evidence-based data on the outcomes.","PeriodicalId":197045,"journal":{"name":"European Journal of Dental and Oral Health","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121506302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Dental Caries and Tooth Loss Prevalence between Diabetic and Non-diabetic Patients: Kabul, Afghanistan 糖尿病和非糖尿病患者龋齿和牙齿脱落患病率的比较:喀布尔,阿富汗
European Journal of Dental and Oral Health Pub Date : 2023-07-03 DOI: 10.24018/ejdent.2023.4.4.281
Ozair Erfan, Hakima Mahmody
{"title":"Comparison of Dental Caries and Tooth Loss Prevalence between Diabetic and Non-diabetic Patients: Kabul, Afghanistan","authors":"Ozair Erfan, Hakima Mahmody","doi":"10.24018/ejdent.2023.4.4.281","DOIUrl":"https://doi.org/10.24018/ejdent.2023.4.4.281","url":null,"abstract":"\u0000Background: Diabetes is a systematic disease that reduces the amount of fluid in the body and causes Xerostomia or dry mouth, which leads to dental caries. Besides, type 2 diabetes is an inflammation-related disease that negatively affects the inflammatory response to dental plaque, leading to intense periodontitis that, if untreated, results in total tooth loss.\u0000\u0000\u0000Aim and Purpose: This study aimed to evaluate the prevalence of dental caries and tooth loss by comparison in diabetic and non-diabetic patients.\u0000\u0000\u0000Material and Method: The cross-sectional study was carried out on 1391 OPD patients over 16 in two governmental hospitals, Kabul University Hospital and National and Specialized Stomatology Hospital. The needed information has been obtained through a questionnaire. Then the oral cavity and the teeth of patients were examined directly in a dental unit in sufficient light and using dental instruments such as dental mirrors and dental explorers. Afterward, data analysis was performed in IBM SPSS Statistic 25.0. \u0000\u0000\u0000Result: The finding of this study shows that 100% of examined patients were suffering from dental caries, and on average, at least there were six teeth lost in each patient. Besides, 55.51% of patients had diabetes with tooth defects. There was 29.92% tooth decay and 25.59% tooth loss, with a remarkable difference between anterior and posterior teeth, as the significant percentage of tooth decay and tooth loss in anterior teeth of diabetic patients than non-diabetics.\u0000","PeriodicalId":197045,"journal":{"name":"European Journal of Dental and Oral Health","volume":"350 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123119538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Treatment of Maxillary Deficiency with Reverse Chin Cup: A Case Report 反颌杯治疗上颌缺陷1例
European Journal of Dental and Oral Health Pub Date : 2023-07-03 DOI: 10.24018/ejdent.2023.4.4.278
Maziar Farhadi, Ali Tashakor, R. Farjaminejad, A. Jamilian
{"title":"Treatment of Maxillary Deficiency with Reverse Chin Cup: A Case Report","authors":"Maziar Farhadi, Ali Tashakor, R. Farjaminejad, A. Jamilian","doi":"10.24018/ejdent.2023.4.4.278","DOIUrl":"https://doi.org/10.24018/ejdent.2023.4.4.278","url":null,"abstract":"\u0000Introduction: This case report aims to illustrate the dentoalveolar changes of a 13-year-old class III patient with maxillary deficiency treated with a Reverse Chin Cup appliance.one of the popular appliances for treating class III patients with deficient maxilla is facemask. In class III growing patients, treatment options include extraoral and intraoral devices. chin and forehead are used for extraoral anchorage supports. \u0000\u0000\u0000Case Presentation: The patient is a 13-year-old girl with a mild maxillary deficiency. She had an Angle Class III molar relationship. She refused to use a face mask due to its highly bulky size. The Reverse Chin Cup is attached to a removable appliance in the upper jaw, with Adams clasps on the first molars, the first premolars, and C-clasps on the upper central and lateral incisors for additional anchorage. \u0000\u0000\u0000Results: After the sixth month of treatment with a chin cup, SNA increased by 2°, and a positive overjet was achieved.\u0000\u0000\u0000Conclusion: This case demonstrates that the reverse chin cup appliance is a suitable alternative to facemasks in class III and maxillary deficient cases.\u0000","PeriodicalId":197045,"journal":{"name":"European Journal of Dental and Oral Health","volume":"07 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128958413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
COVID-19’s Psychosocial Impact on American and Canadian Dental Students COVID-19对美国和加拿大牙科学生的心理社会影响
European Journal of Dental and Oral Health Pub Date : 2023-06-30 DOI: 10.24018/ejdent.2023.4.3.245
D. Schonwetter, Mê-Linh Lê, V. S. Mutalik, Jasbir D. Upadhyaya
{"title":"COVID-19’s Psychosocial Impact on American and Canadian Dental Students","authors":"D. Schonwetter, Mê-Linh Lê, V. S. Mutalik, Jasbir D. Upadhyaya","doi":"10.24018/ejdent.2023.4.3.245","DOIUrl":"https://doi.org/10.24018/ejdent.2023.4.3.245","url":null,"abstract":"\u0000Introduction: The COVID-19 pandemic has significantly affected dental education, contributing to adverse psychological outcomes, especially among dental students. In this cross-sectional study, the psychosocial state of American and Canadian dental students was explored, with special emphasis on affective, behavioral, and cognitive well-being, during and after the initial lockdown. \u0000\u0000\u0000Methods: Dental students were invited to participate in an online survey. The questions evaluated the pandemic’s effect on affective, behavioral, and cognitive responses, and learning experiences. \u0000\u0000\u0000Results: A total of 287 dental students completed the online survey. Sadness and anticipation were the strongest emotions experienced during and following the lockdown. Student worries were classified into external and internal stressors. Females demonstrated higher fear, students from larger dental schools held higher emotions of anticipation and disgust. American students held higher anticipation scores both during and following the lockdown. Canadian, third and fourth years, and students from small dental schools were more aware of which authority to contact if their patient, who presented for care, was suspected of COVID-19 infection which emphasizes the need for a preparedness protocol.\u0000\u0000\u0000Conclusions: More studies that explore the broader scope of psychological aspects are necessary. Monitoring and developing treatment strategies for emergent mental conditions among dental students is extremely important in pandemic crisis management. The study highlights the need for development of standardized protocols and pandemic related health education topics as well as psychological interventions to better prepare dental students globally during the time of crisis.\u0000","PeriodicalId":197045,"journal":{"name":"European Journal of Dental and Oral Health","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133338602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combination Effect of Collagen-Chitosan Hydrogel and Injectable Platelet-Rich Fibrin Fractionation on Osteoblast Migration and Proliferation 胶原-壳聚糖水凝胶联合注射富血小板纤维蛋白分离对成骨细胞迁移和增殖的影响
European Journal of Dental and Oral Health Pub Date : 2023-06-14 DOI: 10.24018/ejdent.2023.4.3.264
Mardha Ade Pritia, N. Fatimah, V. Karina, K. Murdiastuti
{"title":"Combination Effect of Collagen-Chitosan Hydrogel and Injectable Platelet-Rich Fibrin Fractionation on Osteoblast Migration and Proliferation","authors":"Mardha Ade Pritia, N. Fatimah, V. Karina, K. Murdiastuti","doi":"10.24018/ejdent.2023.4.3.264","DOIUrl":"https://doi.org/10.24018/ejdent.2023.4.3.264","url":null,"abstract":"Injectable Platelet Rich Fibrin (i-PRF) is a popular periodontal regenerative therapy in dentistry. i-PRF centrifugation resulted in the fractionation of the yellow layer and buffy coat with a red layer. The red i-PRF contains a greater number of Growth Factor carrier cells than yellow layer, besides that liquid-PRF has a suitable consistency so it easy to combine with other biomaterials. Another biomaterial that can be combined with i-PRF is collagen-chitosan with hydrogel preparations. This study aims to determine the effect of a combination of collagen-chitosan hydrogel formulation with injection of platelet-rich fibrin fractionation on osteoblast cell migration and proliferation. This type of research was an experimental laboratory divided into 3 groups, which were given a combination of the collagen-chitosan hydrogel and i-PRF red layer, a combination of collagen-chitosan hydrogel and i-PRF yellow layer, and only a combination of collagen-chitosan hydrogel. Chitosan collagen hydrogel was prepared with a collagen/chitosan ratio of 25/75, then mixed with i-PRF fractionation with a ratio of 1:1. The cell proliferation test used CCK-8, while the cell migration test used scratch wound healing. Data were tested with One way ANOVA for cell migration and Two way ANOVA for cell proliferation, followed by a Post Hoc test. The results showed that migration of osteoblast cells after 24 hours in the combination group of chitosan collagen hydrogel and i-PRF red layer was higher than the other groups with significance (p<0.05). The results of osteoblast cell proliferation on days 1, 3, and 5 in the combination group of chitosan collagen hydrogel and i-PRF red layer were also higher than the other groups with significance (p<0.05). This study concluded that the combination of chitosan collagen hydrogel with injectable platelet-rich fibrin red layer had a more effect on increasing the migration and proliferation of osteoblast cells than the other groups.","PeriodicalId":197045,"journal":{"name":"European Journal of Dental and Oral Health","volume":"31 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125399714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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