{"title":"Preclinical Study of Human Induced Pluripotent Stem Cell-derived Retinal Pigment Epithelium Cell Sheets Transplantation.","authors":"Hiroyuki Kamao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Age-related macular degeneration is one of the leading causes of blindness characterized by progressive dysfunction of retinal pigment epithelium (RPE) and RPE transplantation. The replacement of pathological RPE with healthy RPE, is being investigated for AMD treatment. In recent years increasing attention has been given to human induced pluripotent stem cells (hiPSCs) as a useful cell source for RPE transplantation. We generated hiPSC-derived RPE (hiPSC-RPE) cell sheets optimized to meet clinical use requiring efficacy, consistency, and safety. These grafts consist of a monolayer of cells without any artificial scaffolds, and express typical RPE markers, form tight junctions that exhibit polarized secretion of growth factors, and show phagocytotic ability and gene expression patterns similar to those of native RPE. Additionally, autologous non-human primate iPSC-RPE cell sheets showed no immune rejection or tumor formation. These results suggest that autologous hiPSC-RPE cell sheets may serve as a useful cell source for RPE transplantation.</p>","PeriodicalId":19670,"journal":{"name":"Nippon Ganka Gakkai zasshi","volume":"120 11","pages":"754-63"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36369421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Regulation of Epithelial Mesenchymal Transition in Ocular Disorders.","authors":"Eri Takahashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Epithelial-mesenchymal transition (EMT) is a process which in epithelial cells lose their character and acquire mesenchymal phenotypes. EMT is associated with inflammation and wound healing; however, aberrant EMT causes fibrosis by disreconstruction of injured tissue and deposition of overproduced extracellular matrix. The adhesion switch from cell-cell contact to cell-extracellular matrix contact occurs during the process of EMT. We found that integrin-associated extracellular matrices induced EMT-like phenomenon via paxillin activation in trabecular meshwork cells. In addition, we discovered that tumor necrosis factor-α is a key EMT inducing factor and hyaluronan receptor CD44 and ERM protein family regulate the induction and maintenance of EMT in retinal pigment epithelial cells. We think that developing drugs based on the concept of EMT may become a new therapy which leads to re-acquisition of epithelial characters and their function in damaged tissues</p>","PeriodicalId":19670,"journal":{"name":"Nippon Ganka Gakkai zasshi","volume":"120 11","pages":"783-90"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36367432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Results and Prognostic Factors for Visual Outcome Following Micro Incision Vitrectomy Surgery in Proliferative Diabetic Retinopathy.","authors":"Hideaki Someya, Tadashi Muraoka, Takayuki Kanda, Masaru Takeuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Purpose: To evaluate outcome of micro incision vitrectomy surgery (MIVS) for proliferative diabetic retinopathy (PDR).\u0000Methods: One hundred seventy three eyes with PDR that underwent primary MIVS were classified into 3 groups by preoperative complications; group A consisted of 56 eyes with only vitreous hemorrhage (VH), group B was 97 eyes with retinal detachment (RD) outside the macula, and group C was 20 eyes with RD both in and out of the macular area. Perioperative factors and visual outcome were compared between 3 groups.\u0000Results: Visual acuity (VA) improved in 117 eyes (68%), remained unchanged in 37 eyes (21%), and deteriorated in 19 eyes (11%). Postoperative VA was improved or not changed in 96% of group A, 88% of group B, and 75% of group C. Postoperative complications were VH (21%), neovascular glaucoma (6%), and RD (5%). Preoperative ocular hypertension, operation time, iatrogenic tear, tamponade, and postoperative complications were significantly more in unimproved eyes, and multivariable analysis revealed preoperative ocular hypertension (odds ratio=5.1) and RD (odds ratio=4.1) as factors of poor postoperative VA.\u0000Conclusion: MIVS is effective for treatment of PDR, but visual outcome resulted from the preoperative conditions.</p>","PeriodicalId":19670,"journal":{"name":"Nippon Ganka Gakkai zasshi","volume":"120 10","pages":"682-8"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36380735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Risk Factors and Preventive Effects of Laser Panretinal Photocoagulation for Neovascular Glaucoma after Ophthalmic Stereotactic Radiotherapy.","authors":"Emi Ueda, Hiroshi Yoshikawa, Satoshi Arakawa, Ri-ichiro Kohno, Saiji Ohga, Katsumasa Nakamura, Tatsuro Ishibashi, Koh-Hei Sonoda","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Purpose: To evaluate the risk factors and the preventive effects of laser panretinal photocoagulation (PRP) for neovascular glaucoma (NVG) after ophthalmic stereotactic radiotherapy.\u0000Methods: Twenty-four patients with ocular malignant tumor (such as uveal malignant melanoma, lacrimal gland cancer) who received stereotactic radiotherapy (such as gamma knife, cyber knife) were retrospectively analyzed. Patients were divided into group A without preventive PRP (n=9), and group B with preventive PRP (n=15). Survival curves were plotted using the Kaplan-Meier method and compared between the two groups with the log-rank test.\u0000Results: In group A, NVG occurred in 3 patients. In contrast, in group B, no patient encountered NVG. The significant risk factor for NVG was dose-volumes that irradiated the optic disk (p=0.045). The incidence of NVG was significantly reduced in group B compared with group A (p=0.019).\u0000Conclusions: Dose-volumes that irradiated the optic disk were risk factors for NVG. PRP is effective in the prevention of NVG.</p>","PeriodicalId":19670,"journal":{"name":"Nippon Ganka Gakkai zasshi","volume":"120 10","pages":"689-95"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36380734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Progress in the Treatment for Intraocular Tumor.","authors":"Yoshiaki Kiuchi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":19670,"journal":{"name":"Nippon Ganka Gakkai zasshi","volume":"120 10","pages":"671-2"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36377331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Change in the Causes Precluding Adherence of Glaucoma Medication Regimen: Systemic Analysis over 12 Months.","authors":"Hiroshi Kobayashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Purpose: To study the change in the causes that precluded adherence to glaucoma medication regimen by repeating the importance of the treatment for 1 year and to analyze them by means of systemic classification.\u0000Methods: Participants in this study were 230 patients who had been prescribed anti- glaucoma medication for at least six months. Adherence was examined at baseline and at 12 month, and its changes were studied. Adherence was ascertained by means of a predetermined questionnaire. Patients with poor adherence were interviewed with stratified questionnaires to elicit the causes precluding the adherence, which were analyzed with cluster analysis.\u0000Results: Two hundred three patients (88.3%) finished this study. Of these, 69 patients reported having poor adherence at baseline and 60 at 12 months (p=0.3). Structured interviews elicited 115 (0.6±0.9/individual) and 79 (0.4±0.7/individual) unique situational problems, respectively (p=0.0128), which were grouped into four separate and major categories:14 and 5 medication regimen-related factors (p=0.0345), 70 and 54 patients-related factors (p=0.1), and 10 and 6 provider-related factors (p=0.3) at baseline and at 12 months, respectively. There were 21 environmental factors at baseline which reduced to 8 after 1 year, and 10 new ones added to them, resulting in a total of 18 (p=0.6).\u0000Conclusions: All situational problems showed a significant reduction after explaining the importance of glaucoma medication. The causes precluding adherence to topical medication regimen may be likely to be multi-dimensional and should be considered in association with each individual patient.</p>","PeriodicalId":19670,"journal":{"name":"Nippon Ganka Gakkai zasshi","volume":"120 10","pages":"673-81"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36380736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Koji Kitazawa, Eri Kondo, Chie Sotozono, Shigeru Kinoshita
{"title":"A Case of Fungal Keratitis Treated Surgically.","authors":"Koji Kitazawa, Eri Kondo, Chie Sotozono, Shigeru Kinoshita","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Purpose: Here we report a patient who underwent removal of a retrocorneal plaque and anterior chamber irrigation for acute-stage fungal keratitis.\u0000Case report: A 56-year-old woman was referred to the Baptist Yamasaki Eye Clinic, Kyoto, Japan due to refractory infectious keratitis. A white plume infiltration from the superficial to deep corneal stroma was present at the central cornea, and a white giant plaque was present on the posterior surface of the cornea. For diagnostic purposes and to reduce inflammation, the retrocorneal plaque was surgically removed and the anterior chamber was irrigated. Findings obtained from the surgically removed plaque revealed many neutrophils coiled with fibrin and filamentous fungus positive to Fungiflora Y staining. At 1-day postoperative, the amount of inflammation and infiltration were drastically decreased and the infection focus became gradually becoming smaller, finally disappearing at 6-weeks postoperative and with no signs of recurrence.\u0000Conclusion: The findings of this study show that surgical intervention can be an effective treatment option for cases of fungal keratitis with retrocorneal plaque.</p>","PeriodicalId":19670,"journal":{"name":"Nippon Ganka Gakkai zasshi","volume":"120 9","pages":"640-5"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36361025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fungal Keratitis:Experience and Evidence, Medical and Surgical Treatment","authors":"Toshihiko Uno","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":19670,"journal":{"name":"Nippon Ganka Gakkai zasshi","volume":"120 9","pages":"629-30"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36359469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Trabeculotomy with Schlemm's Canal Endothelium Removal and Deep Sclerectomy.","authors":"Eriko Yasuda, Akiyasu Kanamori, Kaori Ueda, Azusa Akashi, Mari Sakamoto, Yukako Inoue, Yuko Yamada, Makoto Nakamura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Purpose: To evaluate the effects of trabeculotomy (TLO) combined with Schlemm's canal endothelium removal (SER) and deep sclerectomy (DS).\u0000Method: This retrospective study involved 131 adults eyes, diagnosed with glaucoma that were enrolled with at least 1 year follow-up after TLO. Fifty three eyes received TLO+SER+DS and 78 eyes underwent TLO+DS without SER. SER was performed as peeling of Schlemm's canal endothelium opening under the scleral flap. Surgical success was defined by the need for additional glaucoma surgery, or intraocular pressure (IOP) ≤20 mmHg (criterion A) and ≤16 mmHg (criterion B).\u0000Results: The occurrence rate of transient ocular hypertension (≥30 mmHg) was significantly less (p<0.001) in SER (3.8%) compared with Non-SER (21.8%). SER decreased IOP at 3 years without significant efficacy in terms of lowered IOP compared with Non-SER. At 3 years, Kaplan-Meier survival analysis revealed that the success rate of SER was higher than Non-SER for criterion A (p=0.008), but comparable for criterion B (p=0.06).\u0000Conclusions: SER was effective for reducing the rate of transient ocular hypertension in TLO and controlling IOP≤20 mmHg in adult eyes.</p>","PeriodicalId":19670,"journal":{"name":"Nippon Ganka Gakkai zasshi","volume":"120 9","pages":"635-9"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36361023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Current Status of Glaucoma-related Data Management and Efforts toward Data Standardization.","authors":"Akihiko Oohira","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Purpose: To determine the normative value of the physiologic excyclotorsion angle of the eye using fundus photos (the angle between horizontal line and the line relating fovea and the center of the optic disc), and to analyze the aging effect on this angle.\u0000Methods: 45 and 65 year-old subjects who underwent group glaucoma checkup sponsored by Ohta city government during three years (2013 to 2015) were investigated. Only those without ocular diseases who had good-quality fundus photographs taken were included.\u0000Results: 212 photographs of 39 (45 year-old) and 67 (65 year-old) subjects were analyzed. Physiologic excyclotorsion angle (average±standard deviation) was 6.2±2.9/6.8±2.8 degrees in the right eye and 7.1±3.6/7.8±3.2 degrees in the left eye in the 45/65 year-old groups, respectively. There was no statistical difference between the two groups (t-test. right eye, p=0.27; left eye, p=0.24). The angle of the left eye was statistically larger than of the right eye when the data of both groups were combined (paired t-test, p=0.025).\u0000Conclusions: The angle of the physiologic excyclotorsion determined by fundus photos does not differ between 45 and 65 year-old subjects. The angle of the left eye is larger than the right eye.</p>","PeriodicalId":19670,"journal":{"name":"Nippon Ganka Gakkai zasshi","volume":"120 8","pages":"548-51"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36359471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}