Vera Lúcia Taba, H. C. Carvalho, Túlia de Souza Botelho, A. B. Fernandes, Carlos José de Lima, A. Villaverde
{"title":"Inactivation of Staphylococcus aureus in Surgical Needles by Exposure to Ozone Gas and Low Pressure","authors":"Vera Lúcia Taba, H. C. Carvalho, Túlia de Souza Botelho, A. B. Fernandes, Carlos José de Lima, A. Villaverde","doi":"10.1080/01919512.2021.2021849","DOIUrl":"https://doi.org/10.1080/01919512.2021.2021849","url":null,"abstract":"ABSTRACT Ethylene oxide and gamma irradiation are two well-established sterilizing processes for surgical materials; however, they present toxicity and high cost, respectively. Ozone Gas, O3(g), has been used effectively for disinfection and sterilization. Low pressure (LP) can promote water vaporization whenever the vapor pressure drops below the water saturation pressure, which at 20 °C (experimental temperature) is 2337 Pa. The microorganism used to contaminate the surgical needles (SN) was Staphylococcus aureus, a gram-positive bacterium whose cytoplasm is mostly water. Sixty SN samples were divided into six groups of 10: negative control (sterile SN), positive control (experimentally contaminated SN), LP group, two groups associating LP and O3(g), and O3(g) alone group. The CFU/mL count demonstrated microbicide efficacy of LP+O3(g) and O3(g) groups. This is an innovative protocol for microbial inactivation, mainly because of LP application. In the future, this technique can be evaluated for sterilization of SN and other medical devices.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"21 1","pages":"19 - 27"},"PeriodicalIF":2.7,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83909150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sílvia Móbille Awoyama, H. C. Carvalho, Túlia de Souza Botelho, Sandra Irene Sprogis dos Santos, Debora Alicia Buendía Palacios, Sebastian San Martín Henríque, R. Zângaro, Carlos José de Lima, A. B. Fernandes
{"title":"Disinfection of Human Amniotic Membrane Using a Hydrodynamic System with Ozonated Water","authors":"Sílvia Móbille Awoyama, H. C. Carvalho, Túlia de Souza Botelho, Sandra Irene Sprogis dos Santos, Debora Alicia Buendía Palacios, Sebastian San Martín Henríque, R. Zângaro, Carlos José de Lima, A. B. Fernandes","doi":"10.1080/01919512.2021.2022452","DOIUrl":"https://doi.org/10.1080/01919512.2021.2022452","url":null,"abstract":"ABSTRACT Human amniotic membrane (hAM) has a great potential in regenerative medicine as it can be disinfected, preserved, and stored. The present study evaluated a hydrodynamic system that uses ozonated water for disinfecting hAM and assessed possible morphological alterations using histological analysis and scanning electron microscopy (SEM). hAM fragments were experimentally contaminated with Staphylococcus aureus (103 CFU/mL) and submitted to disinfection processes for 5, 10 and 15 minutes (1.95, 3.9 and 5.85 mg/cm2 (O3), respectively). The results of the water microbiological analysis showed that no microbial growth was detected in all water samples. The hAM surface monitoring with swab method and the analysis of fragment inoculated directly into the culture media revealed that no microbial growth was detected after 10 and 15 minutes. The SEM and histological analysis of hAM revealed that morphological alterations in the epithelium began after 10 minutes 3.9 mg/cm2 (O3) of the disinfection process, and significant changes in the epithelial cells were observed after 15 minutes (5.85 mg/cm2 (O3)). These findings indicates that the structural integrity of hAM was maintained during the process and suggest that the technique is a promising system to disinfect this biological material.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"2 1","pages":"28 - 40"},"PeriodicalIF":2.7,"publicationDate":"2022-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83694220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dalanda Hamdi, Lobna Mansouri, Yu-In Park, V. Srivastava, Mika Sillanpaa, L. Bousselmi
{"title":"Development of a Continuous Photo-catalytic/Ozonation System: Application on Amido Black Removal from Water","authors":"Dalanda Hamdi, Lobna Mansouri, Yu-In Park, V. Srivastava, Mika Sillanpaa, L. Bousselmi","doi":"10.1080/01919512.2021.2004877","DOIUrl":"https://doi.org/10.1080/01919512.2021.2004877","url":null,"abstract":"ABSTRACT A continuous system for photo-catalytic/ozonation was designed at laboratory scale and evaluated for water treatment using Amido Black 10B dye (AB) as a pollutant model. The effect of single ozonation (O3), photolytic ozonation (UV/O3) and combined photocatalytic/ozonation treatment (UV/O3/TiO2) were investigated. TiO2 nanocatalysts films were coated on stainless steel grids (AISI 304) by Atomic Layer Deposition technique (ALD) at 350 °C. An experimental hydrodynamic study of the designed reactor was conducted based on dry and total pressure drop. Three flow regimes were observed by increasing the total pressure drop against gas load factor (Fs). Depollution experiments conducted on the developed reactor showed that reaction rates and efficiency of studied processes were dependent on the studied operational parameters (initial dye concentration, ozone dose, pH). The maximum AB dye ([AB]0 = 100 mg. L−1) conversion efficiency, using the designed (UV/O3/TiO2) system, was about 40.3% of initial TOC and 100% AB color removal was achieved within 10 min at an optimal ozone dose of 132 mg. L−1. A significant improvement in AB removal using UV/TiO2/O3 process (koverall = 0.056 min−1, 40.3%) compared to O3 alone (koverall = 0.032 min−1, 26.87). Results shows also that the addition of tert-butanol (TB), as radical scavenger ([TB] = 0.2 M and 0.4 M), greatly affects the reaction mechanisms of a photocatalytic ozonation process within 5 min of reaction, by decreasing the Amido Black conversion from 99.67% ([TB] = 0 M) to 74.63% and 31.35%, respectively, in presence of 0.2 M and 0.4 M of tert-butanol. The remarkable decrease in AB removal rate observed in the presence of a radical scavenger expresses that there was an obvious enhancement of hydroxyl radical OH• generation due to the synergetic effect between ozone and photocatalytic processes. Graphical abstract","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"1 1","pages":"545 - 565"},"PeriodicalIF":2.7,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89775365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Removal of 2,6-dichloro-1,4-benzoquinone Precursors during Advanced Water Purification Process","authors":"T. Nakai, K. Kosaka, M. Asami, M. Akiba","doi":"10.1080/01919512.2021.2014333","DOIUrl":"https://doi.org/10.1080/01919512.2021.2014333","url":null,"abstract":"ABSTRACT The fates of 2,6-dichloro-1,4-benzoquinone (DCBQ) precursors were evaluated according to their formation potentials (FPs) upon chlorination during ozone/biological activated carbon treatment at two water purification plants (WPPs). DCBQ-FP levels in raw waters were 19 and 21 ng/L. DCBQ-FPs were slightly reduced by coagulation, sedimentation, and rapid filtration, but to < 8 ng/L by ozonation, in contrast to changes in absorbance at 260 nm and trihalomethane (THM)-FPs. DCBQ-FPs of water after sedimentation at WPP and secondary effluents at sewage treatment plants were decreased to a much greater extent than total THM-FPs at low ozone doses. Thus, ozonation is effective for removal of DCBQ precursors.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"9 1","pages":"208 - 216"},"PeriodicalIF":2.7,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85108103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Surface Dielectric Barrier Discharge in a Cylindrical Configuration – Effect of Airflow Orientation to the Microdischarges","authors":"J. Mikeš, S. Pekárek, O. Hanuš","doi":"10.1080/01919512.2021.2016369","DOIUrl":"https://doi.org/10.1080/01919512.2021.2016369","url":null,"abstract":"ABSTRACT This paper is devoted to studying the effect of airflow orientation on the microdischarges of the surface dielectric barrier discharge in cylindrical configuration through varying air input into the discharge chamber, the number of input nozzles, and the geometry of the active electrode. The air is supplied into the discharge chamber tangentially, radially, or axially through one or four input nozzles. Air input into the discharge chamber and the number of input nozzles determine the airflow regime. We used two active electrode geometries that are the axial strips or azimuthal rings. The change of active electrode geometry from rings to strips affects the orientation of microdischarges with respect to the airflow. For the discharge, the variation of air inputs and the number of input nozzles influences the flow regime in the discharge chamber, which, together with the active electrode geometry, affects the temperature field distribution in the chamber. These factors play an important role in plasmachemical processes leading to the discharge generation of various species, such as ozone. It is found that, for the maximum effect of airflow on discharge ozone generation, the streamlines should be uniformly distributed in the discharge chamber and predominantly oriented perpendicular to the majority of microdischarges.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"52 1","pages":"2 - 18"},"PeriodicalIF":2.7,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90796734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Dhevagi, A. Ramya, S. Priyatharshini, R. Poornima
{"title":"Effect of Elevated Tropospheric Ozone on Vigna Mungo L. Varieties","authors":"P. Dhevagi, A. Ramya, S. Priyatharshini, R. Poornima","doi":"10.1080/01919512.2021.2009332","DOIUrl":"https://doi.org/10.1080/01919512.2021.2009332","url":null,"abstract":"ABSTRACT Tropospheric ozone (O3) is widely recognized as the most critical, regional atmospheric pollutant causing significant losses to agricultural productivity due to its phytotoxicity over agricultural areas and is expected to increase in future. In view of rising tropospheric ozone concentration over Indian regions, the present study aimed to evaluate the effect of elevated ozone stress on pulse crop blackgram (Vigna mungo L.), which contributes the major share of protein. The blackgram varieties namely CO 6, VBN 1, VBN 2, VBN 3, VBN 5, VBN 6, VBN 7, and VBN 8 were grown in open top chambers and factorial completely randomized block design was followed. The plants were exposed to elevated ozone concentration (50 and 100 ppb) from 10.00 h to 17.00 h over 10 days at flowering stage, with a weighted average ozone concentration of 50.1 and 101.2 ppb. Both the elevated ozone treatments significantly affected the plant physiological, biochemical, growth, and yield traits of all test varieties. On an average across eight blackgram varieties, decrease in chlorophyll content by 33.83 and 42.41%, stomatal conductance by 28.25 and 40.51% and photosynthetic rate by 29.43 and 42.30% exposed to 50 and 100 ppb ozone were observed, respectively. Correspondingly, the number of pods per plant decreased by 30.82 and 32.65%, 100 grain weight by 7.75 and 21.23% and plant weight by 16.03 and 21.23%, respectively, which were significant at 5% level. Furthermore in the observed traits, significantly higher reduction was observed in VBN3, while the least reduction was observed in VBN8. The path analysis displayed that all the observed physiological, biochemical, growth, and yield traits positively regulated the yield except leaf injury percentage, malondialdehyde, and proline content. The principal component analysis of two elevated ozone treatments confirmed VBN8 as ozone tolerant and VBN3 as ozone sensitive variety. Hence, cultivation of VBN8 variety at ozone hotspot regions would be the best option to overcome ozone induced yield loss.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"51 1","pages":"566 - 586"},"PeriodicalIF":2.7,"publicationDate":"2021-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79051343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Vijay Rakesh Reddy, D. S. Sudhakar Rao, R.R. Sharma, P. Preethi, R. Pandiselvam
{"title":"Role of Ozone in Post-Harvest Disinfection and Processing of Horticultural Crops: A Review","authors":"S. Vijay Rakesh Reddy, D. S. Sudhakar Rao, R.R. Sharma, P. Preethi, R. Pandiselvam","doi":"10.1080/01919512.2021.1994367","DOIUrl":"https://doi.org/10.1080/01919512.2021.1994367","url":null,"abstract":"ABSTRACT Consumers’ awareness toward nutritionally superior foods and improved knowledge of human health have enhanced the inclusion of fruits and vegetables as an integral part of regular dietary intake. However, concerns regarding the safety and quality of foods especially with reference to the outbreak of foodborne illnesses cause a major complication in the consumption of fruits and vegetables. Other major concerns, besides the safety of food from foodborne pathogens, include spoilage due to microbes and chemical pesticide residues. Traditionally, fresh fruits and vegetables are sanitized using chemicals, viz., chlorine, peracetic acid, electrolyzed water, hydrogen peroxide, etc. All these chemicals have been proven to exhibit ill effects over the consumers and the environment over a period of time, and thus, there is a great need for a safe alternative technique, which is eco-friendly and sustainable industrially. Ozone treatment is one such green technology available with multiple benefits such as antimicrobial nature, shelf life extension, pesticide residue removal, starch modification, waste water treatment, and many other industrial applications. It was also approved by FDA as a Generally Recognized As Safe (GRAS) sanitizer because of its eco-friendly nature (degradation into nonharmful oxygen after a short half-life) in addition to its inherent antimicrobial and antiethylene activity. This review focouses on ozone, its mode of action, and its applications in different horticultural crops with potential industrial use.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"12 1","pages":"127 - 146"},"PeriodicalIF":2.7,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73492596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Elimination of Diethylenetriaminepentaacetic Acid from Effluents from Pharmaceutical Production by Ozonation","authors":"Fares Daoud, S. Zühlke, M. Spiteller, O. Kayser","doi":"10.1080/01919512.2021.1983409","DOIUrl":"https://doi.org/10.1080/01919512.2021.1983409","url":null,"abstract":"ABSTRACT During production of diethylenetriaminepentaacetic acid (DTPA), process waste water is generated in several production stages. Process wastewater is usually disposed of via waste water treatment plants. However, due to low biodegradability of DTPA in conventional waste water treatment, incineration constitutes the current method of choice. The main disadvantage of incineration is high consumption of primary energy sources leading to substantial emission of carbon dioxide (CO2). Thus, an alternative method of process waste water treatment was investigated, which consists of an initial application of ozone and a subsequent biological treatment. In 2009, preliminary laboratory experiments were conducted to evaluate the elimination of DTPA in process waste water. Based on the initial results, the responsible authorities granted approval for large-scale ozonation of DTPA-containing wastewater in 2011. Additional laboratory scale experiments were carried out to assess the elimination of the target compound and the generation of its main transformation products using liquid chromatography – high resolution mass spectrometry. Through application of the postulated method, the concentration of DTPA and its derivatives can be reduced to levels assuring safe discharge into the receiving water. Additionally, a comparison of CO2 emissions showed that ozonation is an ecological alternative to incineration and, most likely, an economical as well, based on the local prices of primary energy sources.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"90 1","pages":"473 - 485"},"PeriodicalIF":2.7,"publicationDate":"2021-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86451221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Romeo-Oliván, M. Pagès, Coralie Breton, F. Lagarde, Hubert Cros, O. Yobrégat, F. Violleau, A. Jacques
{"title":"Ozone Dissolved in Water: An Innovative Tool for the Production of Young Plants in Grapevine Nurseries?","authors":"Ana Romeo-Oliván, M. Pagès, Coralie Breton, F. Lagarde, Hubert Cros, O. Yobrégat, F. Violleau, A. Jacques","doi":"10.1080/01919512.2021.1984203","DOIUrl":"https://doi.org/10.1080/01919512.2021.1984203","url":null,"abstract":"ABSTRACT Grapevine trunk diseases can infect nursery plants at different stages of propagation. Here, we evaluated the efficiency of aqueous ozone against two fungi associated with trunk diseases (Phaeoacremonium minimum and Phaeomoniella chlamydospora). The effect of ozone on the plant growth is also studied. Aqueous ozone completely suppressed spore germination in vitro. In planta fungal development was reduced six weeks post-inoculation. In the nursery, ozone treatment was comparable to the usual chemical treatment. Irrigation with aqueous ozone had no negative effects on plant growth. These anti-fungal properties and the absence of phytotoxicity make ozone a promising alternative for controlling microbial infection in nurseries.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"43 1","pages":"521 - 535"},"PeriodicalIF":2.7,"publicationDate":"2021-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72458439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Milagros Basail Pérez, José Efraín González, Vaniert Ventura Chavez, Rosa Elena González Vazquez, O. Portal
{"title":"Water-dissolved Ozone Mediates Optimization of Biotechnological Seed Production of Colocasia esculenta (L.) Schott","authors":"Milagros Basail Pérez, José Efraín González, Vaniert Ventura Chavez, Rosa Elena González Vazquez, O. Portal","doi":"10.1080/01919512.2021.1984207","DOIUrl":"https://doi.org/10.1080/01919512.2021.1984207","url":null,"abstract":"ABSTRACT Taro is a food source for many people in tropical countries. The use of biotechnological techniques for seed production is an alternative that allows obtaining propagation material of higher phytosanitary quality. During the taro in vitro propagation it is of great importance to increase the efficiency of the protocols that are affected mainly by the incidence of microbial contamination and low multiplication coefficients. At the initiation stage, 100% of taro apical meristems sprouted with the immersion in water-dissolved ozone at 2 ppm for 15 min. Furthermore, the microbial contamination was eliminated and the explants showed better quality, increasing the multiplication coefficient in the subsequent subcultures. The disinfection strategy applied allowed to rise the number of taro explants by more than three times, increasing the economic efficiency of the process.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"1 1","pages":"536 - 544"},"PeriodicalIF":2.7,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83060602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}