M Ueda, Y Inada, T Abo, Y Teranishi, A Yamaoka, J Tsutsui, Y Nishimura, A Tanaka
{"title":"[Histopathological and clinical studies of acute necrotizing ulcerative gingivitis and evaluation of treatment].","authors":"M Ueda, Y Inada, T Abo, Y Teranishi, A Yamaoka, J Tsutsui, Y Nishimura, A Tanaka","doi":"10.2329/perio.31.724","DOIUrl":"https://doi.org/10.2329/perio.31.724","url":null,"abstract":"<p><p>We report the clinical data, light and electron microscopic findings, therapy and clinical course of three cases treated for acute necrotizing ulcerative gingivitis. The following results were obtained. 1. An ulcerous lesion was observed in the gingiva of all three cases, with case I also showing a depressed lesion in the ulcerous region. 2. In all three cases, the gingival sections displayed ulceration and showed fibrin deposition and neutrophil infiltration on the surface of the ulcerous region. Congested blood vessels and neutrophil infiltration also were observed in the connective tissue beneath the ulcerous region. 3. Many microorganisms, fibrin, cell debris, and enlarged intercellular spaces of the epithelium were seen on the surface on the ulcerous region. 4. Localized treatment, mainly plaque control, was found to be effective clinically and histopathologically.</p>","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"31 2","pages":"724-35"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2329/perio.31.724","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13778584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Shiraki, T Tanaka, T Hori, K Mizuno, Y Murahashi, Y Iwayama, T Z Tan
{"title":"[Autoradiographic studies on the metabolism of gingival glycosaminoglycans in experimental periodontitis].","authors":"M Shiraki, T Tanaka, T Hori, K Mizuno, Y Murahashi, Y Iwayama, T Z Tan","doi":"10.2329/perio.31.667","DOIUrl":"https://doi.org/10.2329/perio.31.667","url":null,"abstract":"<p><p>The purpose of this study was to investigate the changes in glycosaminoglycans (GAGs) of the gingiva during a period of experimental periodontitis induced by placing a silk ligature below the gingival margin of dog molars. Incorporation of 3H-glucosamine into the gingiva was determined autoradiographically. The gingiva was collected at 0, 7, 21, 60 and 90 days and cultured in vitro in the presence of 3H-glucosamine. The autoradiographs showed a predominantly epithelial location of the silver grains in all gingival epithelia. The location was intercellular in all epithelia. The results suggest greater 3H-glucosamine incorporation by the epithelium compared with the connective tissue and markedly more rapid metabolic turnover of epithelial GAGs.</p>","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"31 2","pages":"667-74"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13780426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Chemiluminescence and phagocytic reactions of human nuclear leukocytes incubated with Streptococcus sanguis].","authors":"N Kobayashi, K Kamoi","doi":"10.2329/perio.31.380","DOIUrl":"https://doi.org/10.2329/perio.31.380","url":null,"abstract":"<p><p>This experiment was designed to determine the role of indigenous organisms in gingival inflammation of the oral cavity, The test bacteria consisted of 3 strains of Streptococcus sanguis isolated from plaque at the gingival margin and grown in sure culture. These strains were subsequently designated as A, B and C. Peripheral nuclear leukocytes were isolated from 5 test subjects with clinically healthy gingiva. Luminol-dependent chemiluminescence and phagocytic functions of the 3 strains were measured, with the following results obtained. The mean peak value of luminol-dependent chemiluminescence following stimulation for strain A was 80.8 in the presence of serum, 38.8 in the presence of inactivated serum, and 20.5 in the absence of serum. For strain B it was 82.3 in the presence of serum, 6.4 in the presence of inactivated serum, and 2.5 in the absence of serum. For strain C it was 68.1 in the presence of serum, 65.3 in the presence of inactivated serum, and 8.3 in the absence of serum. In the absence of plasma strains A and B showed slight phagocytosis. In strain C, however, no phagocytosis was noted. In the presence of inactivated serum phagocytosis was increased strains A and B, but no phagocytosis was noted in strain C. In the presence of blood. phagocytosis was increased in strains A and B, but no phagocytosis was noted in strain C.</p>","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"31 2","pages":"380-402"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13778043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Matsunaga, T Setoguchi, M Nakamura, M Yokota, T Sueda
{"title":"[Relationship between pocket reduction and plaque control in initial treatment].","authors":"M Matsunaga, T Setoguchi, M Nakamura, M Yokota, T Sueda","doi":"10.2329/perio.31.717","DOIUrl":"https://doi.org/10.2329/perio.31.717","url":null,"abstract":"<p><p>It has been suggested that plaque control is important in periodontal treatment. This study used O'Leary's plaque control record (PCR) to investigate the influence of plaque control in initial treatment. Thirty patients (18 men and 12 women, mean age 46.7 years) were selected for this study. The results were as follows: The mean pocket depth was reduced from 3.19 mm (at the first examination) to 2.11 mm (at re-evaluation). Mean pocket depth of 3 mm or more at the first examination was reduced from 4.09 mm to 2.43 mm. The mean number of times plaque control instruction was given to reduce the PCR 10% or less was 3.8. The quantity of pocket reduction was significantly greater in the group that underwent instructions to reduce the PCR 10% or less 4 times or less compared with the group that underwent instructions 5 times or more. The group whose mean PCR was 10% or less from the time 10% or less at the first time to re-evaluation, had significantly greater pocket reduction than the group with 20% or more. There was no correlation between pocket reduction and PCR at the first examination and bone loss scores obtained from X-ray films.</p>","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"31 2","pages":"717-23"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2329/perio.31.717","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13778583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[The interproximal periodontal pocket and its measurement].","authors":"S Hamaguchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Periodontal probing is the most important examination to determine the presence and severity of periodontal lesions. In the interproximal contact areas, probing depth is not accurate because there are some errors in angulating the probe. The purpose of this study was to establish the correct probing method in these areas. Therefore a new interproximal periodontal probe was developed. It eliminated the errors which arise from angulating a probe. The effectiveness of the 8-point method which uses both a conventional probe and the new type interproximal one was determined. The results were as follows: 1. 20 human dry skulls were selected to indicate the necessary angle at which a probe could reach the center of the interproximal contact area. Approximately 30 degrees was needed in molars. This was the most popular angle for measurement in these places. 2. 12 periodontal patients were selected to determine the errors in probing depth between a conventional probe and the new type interproximal one when a probe angulated 30 degrees. The average error was 0.56 mm, indicating that the new type interproximal probe must be used when a probe was angulates 30 degrees. 3. 19 periodontal patients who received a flap operation were selected to determine the effective probing method which could predict the alveolar bone defects in the interproximal contact areas. Measurement of 3 points (bucco-mesial or distal line-angle, linguo-mesial or distal line-angle and center of mesial or distal) could estimate the form of interproximal bone defects. 4. 45 periodontal patients were selected to determine the condition of interproximal periodontal pockets. Marked periodontal damage was present not only at the center of interproximal contact areas but also in the other sites. These results suggest that the new interproximal periodontal probe is a reasonable instrument for angled probing in the interproximal contact areas, and the 8-point method is the most effective one to reveal the periodontal condition in detail.</p>","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"31 2","pages":"608-32"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13780424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N Ogura, T Mera, D Minegishi, C J Lin, R Uraguchi, I Ishikawa
{"title":"[Process of regeneration of cementum observed by various fluorescent-labeling methods].","authors":"N Ogura, T Mera, D Minegishi, C J Lin, R Uraguchi, I Ishikawa","doi":"10.2329/perio.31.633","DOIUrl":"https://doi.org/10.2329/perio.31.633","url":null,"abstract":"<p><p>The purpose of this study was to evaluate the effect of various fluorescent-labeling methods on longitudinal information associated with the process of regeneration of cementum after periodontal therapy. Mandibular bilateral premolars and molars in three monkeys were used. Prior to the labeling, experimental periodontitis was surgically produced at the mesial site of each tooth. In four weeks, scaling and root planing were carried out, and time marking was performed by injection of 3 kinds of fluorescent-labeling materials, tetracycline hydrochloride (30 mg/kg), calcein (8 mg/kg), and alizarin complexon (20 mg/kg), intramuscularly. The animals were sacrificed 4 days after the last injection and serial sections without decalcification were prepared. They were examined under a fluorescence microscope and further observed by contact microradiography (CMR) and staining with toluidine blue. According to the observations made by fluorescence microscopy, marked regeneration of the cementum was revealed by each of the 3 labeling agents. Also, the presence of regenerated cementum was supported by the observations by CMR and after toluidine blue staining. In addition, the regeneration of new cementum was shown to start in about 2 weeks after scaling and root planing. Judging from the results of this experiment, the various fluorescent-labeling methods seem to be effective for observing the process of regeneration of cementum.</p>","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"31 2","pages":"633-9"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13662352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Examination of periodontal disease with gingival crevicular fluid. Correlation between capacitance and clinical findings].","authors":"T Wakao, E Yoshinaga, Y Numabe, K Kamoi","doi":"10.2329/perio.31.573","DOIUrl":"https://doi.org/10.2329/perio.31.573","url":null,"abstract":"<p><p>An intimate relationship between the inflammatory state of the gingiva and the amount of gingival crevicular fluid (GCF) is well known. For the measurement of the volume of GCF, filter paper has been used in the past. In the present study, an electrostatic capacity measuring apparatus with sensor that is insertable into gingival sulcus was fabricated. Capacitance of GCF was measured in patients with periodontitis and the following results were obtained. 1. The measuring apparatus was controlled by a personal computer to obtain the data every 0.1 second. As the GI increased at the site of measurement, higher values were obtained, with longer persistence of the trend of increase in the measured value. 2. In view of the stability of the measured values, the amount of GCF appeared to be best expressed as the value obtained after 10 seconds. 3. The correlation between the capacitance and the clinical findings was evaluated at 500 sites. The correlation was the highest in GI followed by P1I, GBI and PD, suggesting the utility of this method in the detection of initial gingival inflammation.</p>","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"31 2","pages":"573-82"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13841718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Scaler sharpening. Effect of the cutting edge of scaler and the epoxy resin surface with several types of sharpening stones and strokes].","authors":"J Otogoto","doi":"10.2329/perio.31.583","DOIUrl":"https://doi.org/10.2329/perio.31.583","url":null,"abstract":"<p><p>The purposes of this study were to determine the characteristics of a sharpened scaler and planed epoxy resin surface under different sharpening conditions. Each scaler was sharpened with the following kinds of sharpening stones and sharpening strokes by using an originally designed sharpening machine: Stones: 1) Fine India stone (ISF) 2) Arkansas stone (AS) 3) Carborundum stone (CS) 4) ISF followed by AS (ISF + AS) 5) CS followed by AS (CS + AS). Strokes: 1) up strokes (PUSH) 2) down strokes (PULL) 3) up and down strokes (RE) Epoxy resin surfaces were planed (10, 20 and 40 strokes) by sharpened scalers. Before and after these sharpening procedures for each scaler, the cutting edge of each scaler was photographed with a scanning electron microscope (SEM) to determine its roughness and the number of wire edges. The epoxy resin surface was examined with the SEM and the surface roughness was analyzed with a profilometer. The following results were obtained: 1) ISF with three types of sharpening strokes produced the roughest cutting edge. 2) ISF and CS with three types of sharpening strokes produced the largest number of wire edges. 3) PUSH with the five types of sharpening stones showed the roughest cutting edge and the largest number of wire edges. 4) ISF and CS produced more surface roughness, and AS made a smoother surface. Epoxy resin surfaces were planed effectively after 10 strokes. However, no significant differences were found in the surface roughness after 10 to 40 strokes.</p>","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"31 2","pages":"583-92"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2329/perio.31.583","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13841719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[In vivo study of bacterial invasion in root planed and citric acid treated radicular surfaces of periodontally involved human teeth].","authors":"K Ito, N Arai, J Otogoto, S Murai","doi":"10.2329/perio.31.658","DOIUrl":"https://doi.org/10.2329/perio.31.658","url":null,"abstract":"<p><p>The purpose of this study was to investigate whether plaque bacteria invade the exposed radicular dentin after root planing or chemical root treatment in vivo. Eighteen caries-free human periodontally involved teeth with hopeless prognoses were studied. Fourteen teeth were scaled and root planed with hand curette type scalers. The proximal surface of each treated tooth was designated as the RP surface. The remaining half of the proximal surface was treated with citric acid (pH 1.0) for 3 minutes and was designated as the CA surface. Four untreated teeth served as controls. After 4 weeks, the teeth were extracted, and were processed for light microscopy and for scanning electron microscopy concerning bacterial invasion into the supragingival radicular dentin. The following results were obtained. 1. Radicular cementum was present on most untreated tooth surfaces. However, bacteria were never seen in the dentinal tubules. 2. Bacterial invasion into the dentinal tubules was observed in five of the 10 proximal surfaces (50% of the RP surfaces) and in nine of the 10 proximal surfaces (90% of the CA surfaces). 3. The depth (9.5 +/- 24.1 microns vs 84.6 +/- 136.3 microns) and percentage (0.8 +/- 2.1% vs 20.3 +/- 17.3%) of bacterial invasion in the dentinal tubules of the RP surfaces was lower than that of the CA surfaces. 4. Cocci and short rods were present in the supragingival dentinal tubules. 5. Since CA surfaces may accelerate bacterial invasion the citric acid treatment might be harmful in patients with inadequate plaque control.</p>","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"31 2","pages":"658-66"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2329/perio.31.658","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13842452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Effect of various implant materials on cementogenesis].","authors":"M Nagaishi","doi":"10.2329/perio.31.551","DOIUrl":"https://doi.org/10.2329/perio.31.551","url":null,"abstract":"<p><p>Various implant materials have been used to stimulate the regeneration of supporting bone lost from periodontal disease. In addition, the histologic features of bone regeneration associated with their implantation have been evaluated. Very little, however, seems to be known about the effect of implant materials on cementum formation. The aim of this study was to determine whether implant materials stimulate the cementogenesis on adjacent planed root surfaces. Twelve monkeys with healthy gingivae were used in this experiment. Following mucoperiosteal flap elevation, \"windows\" were chiseled in the bone to the proximal root dentin surfaces and adjacent root surfaces were planed. Each of the three implant materials [tricalcium phosphate (TCP), decalcified bone matrix (DBM) and hydroxyapatite (HA)] were then placed in the cuspid and incisor root \"windows\" before the flap was sutured back into the previous position. Windows with no implantation served as a control. Animals were sacrificed 2, 4 and 8 weeks postoperatively. Biopsy specimens including the tooth and surrounding bone were examined by light and electron microscopy. At 2 weeks, all implant particles were surrounded by fibrous tissue. On the other hand, fibrous tissues filled the control defect. On the planed root surfaces after the implantation of TCP and DBM, furthermore, cementoid tissue appeared. At 4 weeks, a considerable amount of new cementum was deposited on the root surfaces except in the implantation of HA. It was especially pronounced after implantation of TCP and DBM which promoted bone regeneration after resorption. These results suggest that resorbable implant materials such as TCP and DBM not only facilitate the formation of new bone, but also of new cementum.</p>","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"31 2","pages":"551-72"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13704326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}