Nihon Shishubyo Gakkai kaishi最新文献

[Distribution of enzymatically pathogenic bacteria from periodontal pocket in advancing periodontitis]. 牙周袋酶致病菌在牙周炎进展中的分布。

Nihon Shishubyo Gakkai kaishi Pub Date : 1990-03-01 DOI: 10.2329/perio.32.199

J Inoue, H Fukushima, T Onoe, T Eryu, M Ueda, A Yamaoka, H Sagawa

引用次数: 2

[Immunohistochemical localization of the chondroitin sulfate proteoglycan in demineralized rat periodontal tissue]. [脱矿大鼠牙周组织硫酸软骨素蛋白多糖的免疫组化定位]。

Nihon Shishubyo Gakkai kaishi Pub Date : 1990-03-01 DOI: 10.2329/perio.32.189

Y Murahashi, T Shibutani, W Nishino, T Hori, M Shiraki, Y Iwayama

{"title":"[Immunohistochemical localization of the chondroitin sulfate proteoglycan in demineralized rat periodontal tissue].","authors":"Y Murahashi, T Shibutani, W Nishino, T Hori, M Shiraki, Y Iwayama","doi":"10.2329/perio.32.189","DOIUrl":"https://doi.org/10.2329/perio.32.189","url":null,"abstract":"We investigated the immunohistochemical localization of the chondroitin sulfates (chondroitin-4 sulfate, -6 sulfate and dermatan sulfate) in demineralized rat periodontal tissue using monoclonal antibodies (2-B-6, 3-B-3). Also, fixative and demineralized methods were established using these monoclonal antibodies. The result showed that the most effective combination of fixative and demineralized methods was 2% glutaraldehyde 1% para-formaldehyde and 5% EDTA. Chondroitin-4 sulfate and dermatan sulfate were widespread in gingival connective tissue and periodontal membrane, with an especially strong response of dermatan sulfate shown along collagen fiber bundles. Chondroitin-6 sulfate was located in peripheral blood vessels. In alveolar bone, chondroitin-4 sulfate and dermatan sulfate were found inside Hareversion canals, Volkman's canals and lacunae. Chondroitin-6 sulfate was localized at peripheral blood in alveolar bone. In cementum, chondroitin-4 sulfate and dermatan sulfate were found at lacunae of cellular cementum and a part of embedding Sharpey's fiber.","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"32 1","pages":"189-98"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2329/perio.32.189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13287451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

[Assessment of alveolar bone changes with gamma-ray absorptiometry--on the analysis of detectability of a measuring system]. [用伽马射线吸收仪评估牙槽骨变化——关于测量系统可检测性的分析]。

Nihon Shishubyo Gakkai kaishi Pub Date : 1990-03-01 DOI: 10.2329/perio.32.214

N Koura, S Fujihara, M Yokota, T Sueda, T Noikura

引用次数: 0

[A rapid diagnosis for periodontitis based on the enzymatic activity of periodontopathic bacteria. The application of the SK-013 to the judgement on the effect of initial preparation]. 基于牙周病细菌酶活性的牙周炎快速诊断。【SK-013对初步准备效果判断的适用】。

Nihon Shishubyo Gakkai kaishi Pub Date : 1990-03-01 DOI: 10.2329/perio.32.249

T Kobayashi, E Satoh, N Sugita, K Okuda, M Yanagimura, H Yoshie, K Hara, C Sakurai, C Kurihara, N Minamizaki

{"title":"[A rapid diagnosis for periodontitis based on the enzymatic activity of periodontopathic bacteria. The application of the SK-013 to the judgement on the effect of initial preparation].","authors":"T Kobayashi, E Satoh, N Sugita, K Okuda, M Yanagimura, H Yoshie, K Hara, C Sakurai, C Kurihara, N Minamizaki","doi":"10.2329/perio.32.249","DOIUrl":"https://doi.org/10.2329/perio.32.249","url":null,"abstract":"The purpose of the present study was to examine the diagnostic value of the SK-013 (Sunstar, Osaka) in evaluating the effect of initial preparation. The SK-013 is a highly sensitive reagent to measure peptidase activity specifically produced by Bacteroides gingivalis. Bacteroides forsythus, and Treponema denticola. Thirty-six sites in 16 periodontitis patients were monitored in this study. The clinical status of each site was assessed using the clinical indices such as gingival index (GI), plaque index (PII), probing depth (PD), and bleeding on probing (BOP). The composition of subgingival microflora in samples was determined using phase contrast microscopy. Clinical, microbiological, and enzymatic examinations were made at five separate appointments, as follows: (1) at baseline prior to plaque control instruction; (2) 4 weeks following plaque control instruction; and (3) 2, 4, 8 weeks following scaling and root planing of periodontal sites. The results of these examinations were compared statistically. The correlation was positive between the total counts of bacteria including spirochetes and motile rods and the enzymatic activity identified using the SK-013. Also the enzymatic profile was highly correlated with microbiological findings other than clinical indices. These results show that the SK-013 is a clinically useful diagnostic method for assessing the effect of initial periodontal therapy.","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"32 1","pages":"249-60"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13287457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

[A rapid diagnosis (SK-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria. The relationship between the enzymatic activity (SK-013) and B. gingivalis, B. forsythus, T. denticola in subgingival microflora]. 基于牙周病细菌酶活性的牙周炎快速诊断(SK-013)。龈下菌群中酶活性(SK-013)与牙龈双歧杆菌、连翘双歧杆菌、牙齿双歧杆菌的关系[j]。

Nihon Shishubyo Gakkai kaishi Pub Date : 1990-03-01 DOI: 10.2329/perio.32.233

K Seida, A Saito, S Yamada, M Tsunoda, T Sato, K Ishihara, Y Naito, I Takazoe, K Okuda

{"title":"[A rapid diagnosis (SK-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria. The relationship between the enzymatic activity (SK-013) and B. gingivalis, B. forsythus, T. denticola in subgingival microflora].","authors":"K Seida, A Saito, S Yamada, M Tsunoda, T Sato, K Ishihara, Y Naito, I Takazoe, K Okuda","doi":"10.2329/perio.32.233","DOIUrl":"https://doi.org/10.2329/perio.32.233","url":null,"abstract":"SK-013 was developed for rapid detection of the peptidase activity in subgingival plaque samples. The purpose of this study was to determine whether SK-013 could be a marker for the presence of periodontopathic bacteria including Treponema denticola, Bacteroides gingivalis and Bacteroides forsythus which produce trypsin-like enzyme. Subgingival plaque samples were taken with 3 paper points from 10 clinically healthy sites and 30 periodontal diseased sites. The SK-013 activity of plaque sample was assayed and the cells of T. denticola, B. gingivalis and B. forsythus in the sample were counted by immunofluorescence technique. In diseased sites, both the SK-013 activity and the cell counts of these organisms were significantly higher than those in healthy sites. The proportions and cell counts of these organisms and the SK-013 activity were closely correlated with clinical parameters including Gingival Index, Plaque Index, and Probing depth. The correlation between the presence of these organisms and the SK-013 activity was significant. Correlation coefficients between the presence of T. denticola and the SK-013 activity were higher than those of others.","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"32 1","pages":"233-40"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2329/perio.32.233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13287455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[A study of ultrasonic scaling in combination with povidone-iodine solution. (1)]. 聚维酮-碘溶液联合超声除垢的研究。(1)]。

Nihon Shishubyo Gakkai kaishi Pub Date : 1990-03-01 DOI: 10.2329/perio.32.309

M Ueda, Y Teranishi, M Yamamoto, S Ohno, R Kobayashi, C Ogata, S Ushijima, N Nakagaki, H Kawasaki, E Kawashima

引用次数: 0

[Friction sound as an objective sign of completion of root planing]. [摩擦声作为刨根完成的客观标志]。

Nihon Shishubyo Gakkai kaishi Pub Date : 1990-03-01 DOI: 10.2329/perio.32.129

M Suzuki

{"title":"[Friction sound as an objective sign of completion of root planing].","authors":"M Suzuki","doi":"10.2329/perio.32.129","DOIUrl":"https://doi.org/10.2329/perio.32.129","url":null,"abstract":"Root planing is one of the most important procedures in periodontal treatment. However, there are no objective clinical signs to indicate completion of this procedure. The purpose of this study was to examine the friction sound produced by curettes and root surfaces during root planing and to test the possibility of utilizing the sound changes as an objective parameter for evaluating completion of the procedure. The study consisted of a clinical and laboratory experiment. Seven periodontally diseased teeth were utilized in the clinical trial. During scaling and root planing with the Gracey curette, the friction sound was clinically analyzed with a sound spectrograph. The root surface, in situ, was observed by scanning electron microscopy (SEM) with the replica technique. Twenty-one periodontally diseased teeth were used in the laboratory study. Root planed surfaces were classified as pre-root planed and post-root planed according to their friction sounds during root planing. Each surface was examined by the following methods: 1) measuring root surface roughness 2) SEM observations 3) determination of number of adherent cells by the cultured fibroblast technique. As a result, post-root planed surfaces were well root planed as evaluated by SEM observations and the profilometer. Similarly, adherent cell counts were increased on post-root planed surfaces. These results indicated the possible usefulness of friction sound analysis to judge the completion of root planing.","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"32 1","pages":"129-39"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13288091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Histo-pathology of the regenerating process in the tissue around the periodontally diseased teeth of beagle dogs. Clinical and histological analysis following flap surgery]. 比格犬牙周病变牙齿周围组织再生过程的组织病理学研究。皮瓣术后临床及组织学分析[j]。

Nihon Shishubyo Gakkai kaishi Pub Date : 1990-03-01 DOI: 10.2329/perio.32.150

T Ogawa, H Hirohata, H Kawaguchi, Y Sugano, M Kouchi, H Satoh, M Shirakawa, H Okamoto

{"title":"[Histo-pathology of the regenerating process in the tissue around the periodontally diseased teeth of beagle dogs. Clinical and histological analysis following flap surgery].","authors":"T Ogawa, H Hirohata, H Kawaguchi, Y Sugano, M Kouchi, H Satoh, M Shirakawa, H Okamoto","doi":"10.2329/perio.32.150","DOIUrl":"https://doi.org/10.2329/perio.32.150","url":null,"abstract":"The regenerating process in tissue around periodontally diseased teeth (PDTs) and non-diseased teeth (NDTs) was studied with clinical and histological analysis following flap surgery. PDTs were prepared in the premolars of 7 beagle dogs by which surgically denuding the root surfaces by removing the alveolar bone. The denuded sites were covered with gutta-percha plates and gingival epithelial tissues and exposed to the oral environment for 4 weeks. Then flap surgery was performed on the PDTs. As control sites, the root surfaces of the NDTs were denuded by removing the alveolar bone at the time of flap surgery. The root surfaces of the PDTs and NDTs were planed with curette scalers at one root site but not at the other site. Clinical and histopathological findings were evaluated at fixed intervals for 32 weeks after surgery. Results of observation were as follows. 1. In the clinical evaluations, the gingival inflammation index, probing depth and attachment level were improved in the root planing (PR) group of both the PDTs and the NDTs, but in the non RP group of PDTs there was no improvement. 2. In the histologic observation, the position of the gingival margin, length of regenerated junctional epithelium, depth of gingival sulcus and the level of connective tissue attachment in the RP groups recovered in both the PDTs and the NDTs but not in the non RP groups.","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"32 1","pages":"150-63"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13288093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

[A rapid diagnosis for periodontitis based on enzymatic activity of periodontopathic bacteria. Correlation between enzymatic activity, clinical periodontal parameters, and subgingival level of microorganisms]. 基于牙周病细菌酶活性的牙周炎快速诊断。酶活性、临床牙周参数和龈下微生物水平之间的相关性[j]。

Nihon Shishubyo Gakkai kaishi Pub Date : 1990-03-01 DOI: 10.2329/perio.32.241

T Ohtake, C Sakurai, C Kurihara, N Minamizaki, M Kobayashi, H Miyashita, K Hasegawa, T Kobayashi, E Satoh, N Sugita

{"title":"[A rapid diagnosis for periodontitis based on enzymatic activity of periodontopathic bacteria. Correlation between enzymatic activity, clinical periodontal parameters, and subgingival level of microorganisms].","authors":"T Ohtake, C Sakurai, C Kurihara, N Minamizaki, M Kobayashi, H Miyashita, K Hasegawa, T Kobayashi, E Satoh, N Sugita","doi":"10.2329/perio.32.241","DOIUrl":"https://doi.org/10.2329/perio.32.241","url":null,"abstract":"There have been various laboratory methods for the microbiological diagnosis of periodontal disease. However, there have been some disadvantages in these methods. In this study of the application of a chair-side test for microbiological diagnosis, the activity of peptidases in periodontal pockets of patients was examined by using the assay system SK-013. SK-013 consists of synthetic substrates and is capable of rapidly (in 15 min) evaluating the activity of specific peptidase from Treponema denticola and Bacteroides species. Using SK-013, we evaluate the correlation between the enzymatic activity, clinical periodontal parameters and subgingival level of microorganisms, including phase contrast microscopy. We calculated the sensitivity and efficacy of SK-013 as a diagnostic indicator in the presence of Spirochetes and in periodontitis. A positive correlation were demonstrated between enzymatic activity, clinical periodontal parameters, the numbers of total cell count, Spirochetes, and M & S ratio. SK-013 was highly sensitive and efficacious (sensitivity: 92%, efficacy: 96%). We concluded that the assay system SK-013 is a useful chair-side method for diagnosing periodontal disease.","PeriodicalId":19428,"journal":{"name":"Nihon Shishubyo Gakkai kaishi","volume":"32 1","pages":"241-8"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13287456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[A rapid diagnosis (SK-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria. Study on the characterization of the SK-013 and the enzyme specificity]. 基于牙周病细菌酶活性的牙周炎快速诊断(SK-013)。SK-013的鉴定及酶特异性研究[j]。

Nihon Shishubyo Gakkai kaishi Pub Date : 1990-03-01 DOI: 10.2329/perio.32.224

K Ishihara, Y Naito, T Kato, I Takazoe, K Okuda, T Eguchi, K Nakashima, N Matsuda, K Yamasaki, K Hasegawa

引用次数: 0