Notulae Scientia Biologicae最新文献_第3页

Extraction of phenolic and flavonoid compounds and evaluation of their antioxidant activity in saffron anthers (Crocus sativus L.) 提取藏红花花药中的酚类和类黄酮化合物并评估其抗氧化活性

Notulae Scientia Biologicae Pub Date : 2023-11-15 DOI: 10.55779/nsb15411640

Huda E. Mahood, Ali Amer Dahham, Virginia Sarropoulou, Thiresia-Teresa Tzatzani

{"title":"Extraction of phenolic and flavonoid compounds and evaluation of their antioxidant activity in saffron anthers (Crocus sativus L.)","authors":"Huda E. Mahood, Ali Amer Dahham, Virginia Sarropoulou, Thiresia-Teresa Tzatzani","doi":"10.55779/nsb15411640","DOIUrl":"https://doi.org/10.55779/nsb15411640","url":null,"abstract":"Saffron (Crocus sativus L.) is the most expensive spice in the world and rich in a variety of bioactive compounds including phenolic acids, flavonoids, and vitamins. Saffron is used in food, cosmetics, perfumery, and dye industries due to its color, taste, aroma, and medicinal properties. The extraction of bioactive compounds imposes a constant search for economically and environmentally viable extraction strategies for higher yields including the solvent type. The research was carried out to evaluate total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of C. sativus L. anthers using different extraction solvents including ethanol, methanol, and distilled water. The results showed that ethanol was the most effective extraction solvent type exhibiting the highest TPC (7.29 mg GAE g-1 DW), TFC (3.77 mg QE g-1 DW), and antioxidant activity of flavonoids (88%) and ascorbic acid (76.07%). Flavonoids proved to be stronger antioxidants than ascorbic acid, irrespective of solvent type, however the ethanolic extracts showed significantly higher antioxidant activity. A Reverse Phase High-Performance Liquid Chromatography method revealed that gallic acid (3.1 mg g-1), syringic acid (0.2 mg g-1), and vanillic acid (0.13 mg g-1) were the main phenolic compounds detected in the dried anther ethanolic extracts, while quercetin (2.13 mg g-1), pyrogallol (1.73 mg g-1), kaempferol (1.2 mg g-1), rutin (0.2 mg g-1), and tricin (0.1 mg g-1) the main flavonoid compounds. Thus, apart from the spice, constituted by the red stigmas, the production of other flower parts such as the anthers, so far considered agricultural waste can constitute a rich source of bioactive compounds of high antioxidant potential by setting quality standards for new products’ development and ensuring better valorization of saffron’s bioresidues in Mashhad region of Iran.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"10 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139274716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insecticidal potential of Streptomyces sp. dichloromethane extracts against the cactus cochineal Dactylopius opuntiae (Cockerell) 链霉菌二氯甲烷提取物对仙人掌鞘氨醇 Dactylopius opuntiae (Cockerell) 的杀虫潜力

Notulae Scientia Biologicae Pub Date : 2023-11-15 DOI: 10.55779/nsb15411574

Said Rammali, Mohamed El Aalaoui, M. Sbaghi, Khadija Dari, B. Bencharki, Abdelhamid Azeroual, A. Khattabi

{"title":"Insecticidal potential of Streptomyces sp. dichloromethane extracts against the cactus cochineal Dactylopius opuntiae (Cockerell)","authors":"Said Rammali, Mohamed El Aalaoui, M. Sbaghi, Khadija Dari, B. Bencharki, Abdelhamid Azeroual, A. Khattabi","doi":"10.55779/nsb15411574","DOIUrl":"https://doi.org/10.55779/nsb15411574","url":null,"abstract":"Destructive damages caused by Dactylopius opuntiae (Cockerell) to cactus worldwide require an ecofriendly IPM approach. Streptomyces sp. produce wide range of biologically active secondary metabolites that can be an interesting alternative to chemical insecticides for pest control, as they are less toxic and easily biodegradable. The efficacy of dichloromethane extracts of four Moroccan Streptomyces sp. strains: Streptomyces bellus- E23-2, Streptomyces galilaeus- E23-9, Streptomyces africanus- E23-3, and Streptomyces bellus- E25-12 (applied at 11, 13, 15, 17 and 20 mg mL-1) against D. opuntiae nymphs and adult females was evaluated under laboratory and greenhouse conditions. Results showed that Streptomyces bellus- E23-2 and Streptomyces galilaeus- E23-9 dichloromethane extracts applied at 20 mg mL-1 were more effective, causing higher mortality against nymphs (92% and 91%, respectively) and adult females (90% and 95%, respectively) after 8 days of exposure, resulting in an LT50 value of 3.0 days (nymph), and 3.0 and 6.0 days (adult female), respectively. Streptomyces bellus- E25-12 extract had the lowest mortalities [88% (nymph) and 68% (adult female)]. In greenhouse experiment, the highest first instar nymph mortality was achieved by Streptomyces bellus- E23-2 (55.5%) and Streptomyces galilaeus- E23-9 (50.5%) dichloromethane extracts at 20 mg mL-1. The metabolites found in dichloromethane extracts of Streptomyces bellus- E23-2 and Streptomyces galilaeus- E23-9 show considerable potential to be used in the development of new biopesticide formulations for use in integrated pest management programs against D. opuntiae.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"EM-31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139275694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of lipases and preliminary purification of tributyrinesterases from lactic acid bacteria strains belonging to the Enterococcus and Lactococcus genera 属于肠球菌属和乳球菌属的乳酸菌菌株中脂肪酶的特征和三丁酯酶的初步纯化

Notulae Scientia Biologicae Pub Date : 2023-11-15 DOI: 10.55779/nsb15411634

Amina Dellali, Faiza Boublenza, H. Zadi-Karam, N. Karam

{"title":"Characterization of lipases and preliminary purification of tributyrinesterases from lactic acid bacteria strains belonging to the Enterococcus and Lactococcus genera","authors":"Amina Dellali, Faiza Boublenza, H. Zadi-Karam, N. Karam","doi":"10.55779/nsb15411634","DOIUrl":"https://doi.org/10.55779/nsb15411634","url":null,"abstract":"The objective of this study is to characterize and attempt to purify lipolytic enzymes derived from strains of Enterococcus and Lactococcus genera isolated from various environments. For this purpose, ten strains of lactic acid bacteria were studied for their lipolytic and esterasic activities on an MRS (Man, Rogosa, and Sharpe) medium containing natural and/or artificial lipid substrates. Among them, two strains: Lactococcus lactis subsp lactis and Lactococcus lactis subsp cremoris showed maximum extracellular lipolytic activity in the presence of 1% olive oil. It was observed that both strains exhibited higher lipolytic activity at pH 7 and 8, with an optimal temperature of 30 °C and 37 °C, respectively. Glucose was found to be the best carbon source for both tested strains. The study of growth kinetics and fatty acid production over time revealed that fatty acid production begins during the exponential phase and reaches its maximum during the stationary phase. The purification of tributyrinesterases was carried out on two strains of Enterococcus genus: Enterococcus feacium and Enterococcus durans through ammonium sulfate precipitation and Sephadex G-100 gel filtration chromatography allowed the estimation of the molecular weight of the tributyrinesterases as 32.09 kDa and 38.49 kDa for both strains, respectively.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139272937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introduction pages, Notulae Scientia Biologicae 15(3), 2023 介绍页，《生物科学通报》15（3），2023 年

Notulae Scientia Biologicae Pub Date : 2023-09-29 DOI: 10.55779/nsb15311732

Radu E. SESTRAS

{"title":"Introduction pages, Notulae Scientia Biologicae 15(3), 2023","authors":"Radu E. SESTRAS","doi":"10.55779/nsb15311732","DOIUrl":"https://doi.org/10.55779/nsb15311732","url":null,"abstract":"Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 3, Volume 15, 2023: The papers published in this issue represent interesting novelties in different topics of life science. Among the exciting research or reviews, we invite readers to find news about: Acute oral toxicity of Cannabis sativa L. co-products in mice; Modulation of antioxidant activities, markers of hepatic and renal dysfunctions in alloxan-induced diabetic rats by Combretum dolichopetalum; Phytochemical screening, total phenolic content and antioxidant activities of Marrubium vulgare L. collected in three development stages; Comparison of different drying methods for phytochemical quality of stevia (Stevia rebaudiana Bert.); Evaluation of reproduction biology of Prunus cerasoides; Edible mushrooms – perspectives and considerations; Production of an important antidiabetic compound mangiferin through elicitation in Salacia chinensis under in vivo condition; Analysis of the antibacterial potentials of the leaf extracts of selected medicinal plants against pathogenic bacterial strains; Phytochemical fingerprinting and evaluation of in silico anti-thrombotic properties of Justicia adhatoda L. and Cordia dichotoma Frost.; Extraction of nutrients from Rumex vesicarius, a wild indigenous edible plant from United Arab Emirates; Effects of pre-harvest chitosan application on growth parameters and total phenolic - antioxidant contents of bitter gourd (Momordica charantia L.).","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135195142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of NPK fertilizer on the biochemical response of tomatoes (Solanum lycopersicum L.) 氮磷钾肥对番茄生化反应的影响

Notulae Scientia Biologicae Pub Date : 2023-09-28 DOI: 10.55779/nsb15311516

Zoubida BENTAMRA, Housna MEDJEDDED, Saïd NEMMICHE, Mohammed BENKHELIFA, Danilo R. DOS SANTOS

{"title":"Effect of NPK fertilizer on the biochemical response of tomatoes (Solanum lycopersicum L.)","authors":"Zoubida BENTAMRA, Housna MEDJEDDED, Saïd NEMMICHE, Mohammed BENKHELIFA, Danilo R. DOS SANTOS","doi":"10.55779/nsb15311516","DOIUrl":"https://doi.org/10.55779/nsb15311516","url":null,"abstract":"Increasing nutrient inputs affect plants and soil. Long-term, repeated mineral fertilizer applications may alter the agro-ecosystem. The application of the right amount of fertilizer is primordial to maintaining and improving sustainable plant productivity. The physiological response of the plant provides meaningful information on yield and makes it possible to better choose the doses of fertilization amendments. In order to determine the adequate dose of NPK fertilizer under economically profitable and more environmentally sustainable conditions, we investigated the impact of the chemical fertilizer amended at different NPK fertilizer doses (15, 20, and 25 kg ha-1) on the growth parameters, oxidative metabolism, and yield of Solanum lycopersicum. A complete random block experimental set-up with three doses and four replicates was performed for a greenhouse tomato crop. The results showed that NPK fertilizer influences morphological parameters, and phenolic compounds with the best data correlated to the medium treatment. Likewise, the content of flavonoids, lycopene, and β-carotene in tomato fruits displays a similar trend of variation with all treatment doses. The biochemical responses of plants to mineral fertilizer indicate that a medium dose is suitable, without overuse of fertilizers and pesticides, to reduce the negative impact on the agro-ecosystem.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135426170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction of nutrients from Rumex vesicarius, a wild indigenous edible plant from United Arab Emirates 从阿拉伯联合大公国的一种野生可食植物——水仙花中提取营养成分

Notulae Scientia Biologicae Pub Date : 2023-09-28 DOI: 10.55779/nsb15311658

Reem A.S. ALKAABI, Hasan H. BANI MATAR, Kandhan KARTHISHWARAN, Zienab F.R. AHMED, Shyam KURUP, Mohammed SALEM ALYAFEI, Abdul JALEEL

引用次数: 0

Biopesticide and biofertilizer potential of tropical earthworm vermicast tea 热带蚯蚓蚓茶的生物农药和生物肥料潜力

Notulae Scientia Biologicae Pub Date : 2023-09-28 DOI: 10.55779/nsb15311343

Ebenezer O. DADA, Simeon O. NWANI, Sekinat M. YUSUFF, Yusuf O. BALOGUN

{"title":"Biopesticide and biofertilizer potential of tropical earthworm vermicast tea","authors":"Ebenezer O. DADA, Simeon O. NWANI, Sekinat M. YUSUFF, Yusuf O. BALOGUN","doi":"10.55779/nsb15311343","DOIUrl":"https://doi.org/10.55779/nsb15311343","url":null,"abstract":"The adverse effects of chemical pesticides have continued to drive the search for safe, biological alternatives. Studies on biopesticide potential of earthworm casts have remained largely limited to those of temperate earthworms. We evaluated the insect pest repellency and growth-promoting potential of tropical earthworm-derived vermicast tea on the seedlings of Arachis hypogaea (groundnut), Zea mays (maize) and Phaseolus vulgaris (bean). Field-sourced earthworm casts were soaked in water for 48 hours, routinely stirred every 6 hours, and filtered through a fine mesh cloth. The filtrate was the vermicast tea. Seedlings grown in garden soil were sprayed with vermicast tea every four days. The seedlings were monitored for insect pest-induced leaf damage and growth performance for 5 weeks. Vermicast tea exhibited insect pest repellency effect on groundnut and bean seedlings, as evidenced by the significantly lower (p<0.01) insect pest attack on the treated seedlings, as against the untreated that recorded high pest infestations. However, leaf damage was relatively low in maize seedlings, and the differences in percentage leaf damage among the treated and untreated were not significant (p>0.05). The effect of vermicast tea on the physical growth of seedlings was positive, but marginal. This result calls for increased research on tropical earthworms.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135426016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short-term changes in antioxidant response of Eutrema salsugineum exposed to severe salinity 高盐度条件下梭鲈抗氧化反应的短期变化

Notulae Scientia Biologicae Pub Date : 2023-09-27 DOI: 10.55779/nsb15311558

Hasna ELLOUZI, Mokded RABHI, Mohsen HANANA

引用次数: 0

Effect of time of treatment on the efficacy of trypanocides in rats experimentally infected with Trypanosoma brucei brucei 布氏锥虫实验感染大鼠后，治疗时间对杀锥虫药物疗效的影响

Notulae Scientia Biologicae Pub Date : 2023-09-27 DOI: 10.55779/nsb15311556

Ikenna O. EZEH, Chijioke N. IHEAGWAM, Chukunonso F. OBI, Michael I. OKPALA, Ganiyu E. ANERU, Charles O. AGBAEZE, Romanus C. EZEOKONKWO, Denchris ONAH

{"title":"Effect of time of treatment on the efficacy of trypanocides in rats experimentally infected with Trypanosoma brucei brucei","authors":"Ikenna O. EZEH, Chijioke N. IHEAGWAM, Chukunonso F. OBI, Michael I. OKPALA, Ganiyu E. ANERU, Charles O. AGBAEZE, Romanus C. EZEOKONKWO, Denchris ONAH","doi":"10.55779/nsb15311556","DOIUrl":"https://doi.org/10.55779/nsb15311556","url":null,"abstract":"The effect of treatment time on the efficacy of trypanocides in rats experimentally infected with Trypanosoma brucei brucei was assessed. The thirty albino rats utilized in this study were divided into six groups, each with five rats. Group 1 rats were uninfected while those in groups 2 - 6 were inoculated intraperitoneally with 106 trypanosomes. Group 2 rats were left untreated while groups 3 - 6 rats were treated respectively on days 5, 8, 11 and 14 post infection (PI) with 1.0 mg/kg Securidium® (brand of isometamidium chloride) intraperitoneally. Level of parasitaemia, rectal temperature, clinical signs, haematological indices (packed cell volume and total leucocyte count) and survivability were used to evaluate the treatment time on the efficacy of Securidium®. Pre-patent period was 4 - 5 days. On days 4, 5, and 11 following the treatments (PT), the parasites were eliminated in the treated groups 3 - 6 respectively. Also, two rats relapsed in group 3 from 37 days PT with 20% mortality, whereas groups 4 - 6 rats relapsed from days 19, 16 and 21 PT respectively. Groups 4 - 6 rats had 40%, 60% and 80% mortalities respectively. The mean PCV and TLC showed a striking drop (p<0.05) PI, however, group 3 had a better result PT compared to other infected-treated groups. In conclusion, it is safe to say that treatment time is a significant determinant of therapeutic efficacy in trypanosomosis, as early treatment group had better overall indices of therapeutic efficacy than the late treatment groups.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"150 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135586502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary dysmenorrhea among adolescents in the District of Constantine, Algeria 阿尔及利亚康斯坦丁地区青少年的原发性痛经

Notulae Scientia Biologicae Pub Date : 2023-09-27 DOI: 10.55779/nsb15311633

Nousseiba ABED, Hadjer MARZOUG, Kenza BOUGHAMBOUZ

{"title":"Primary dysmenorrhea among adolescents in the District of Constantine, Algeria","authors":"Nousseiba ABED, Hadjer MARZOUG, Kenza BOUGHAMBOUZ","doi":"10.55779/nsb15311633","DOIUrl":"https://doi.org/10.55779/nsb15311633","url":null,"abstract":"To determine the frequency of dysmenorrhea, its properties and its impact, behaviour of adolescents in the District of Constantine, and to explore the relationship between dysmenorrhea and several presumed risk factors. A cross-sectional study was employed in 562 adolescent girls. A multiple-choice questionnaire was administered to the adolescents. The severity of dysmenorrhea pain was assessed by visual analogy scale. Data were obtained and analysed using SPSS version 22. In the sample, the frequency of dysmenorrhea was 89.7%. In 55.6% of cases the pains appeared after the 1st year of menstruation. The mean intensity of pain was 5.64 + 3.02. Of all adolescents experiencing dysmenorrhea, 51.2% reported moderate pain. 58.7% reported the duration of their menstrual pain as 24 hours or less. 47.30% experienced pain most at onset of menses. A variety of signs were reported mostly nervousness (65.6%), headache (45.9%); depressed mood (35.8%) and painful breast (32.4%). 89.7% reported missing. Only 12.4% of the adolescents consulted a doctor for their dysmenorrhea; 44.7% used a medical prescription and self-medication. The most common medications used were Paracetamol, Phloroglucinol and Diclofenac potassium. 74.5% used herbal treatment to manage their dysmenorrhea. The results show that risk factors that showed significant association with pain severity were family history, duration of menstrual period, nature of menstrual flow and the psychological profile. The frequency of dysmenorrhea among the District of Constantine is high, and the pain that these adolescents suffer can be severe, disabling. Our results suggest that family history, duration and nature of menstrual flow and the psychological profile are important risk factors associated with dysmenorrhea.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135586493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0