North American Journal of Aquaculture最新文献

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Susceptibility of Delta Select and Delta Control Channel Catfish lines to experimental Edwardsiella ictaluri and Edwardsiella piscicida infection Delta Select 和 Delta Control 鲴鱼品系对实验性 Edwardsiella ictaluri 和 Edwardsiella piscicida 感染的易感性
IF 1 4区 农林科学
North American Journal of Aquaculture Pub Date : 2024-04-11 DOI: 10.1002/naaq.10338
Brian G. Bosworth, Manoj Chandy Koshy, Cynthia C. Ware, Fernando Y. Yamamoto, Todd S. Byars, Matt J. Griffin, David J. Wise
{"title":"Susceptibility of Delta Select and Delta Control Channel Catfish lines to experimental Edwardsiella ictaluri and Edwardsiella piscicida infection","authors":"Brian G. Bosworth, Manoj Chandy Koshy, Cynthia C. Ware, Fernando Y. Yamamoto, Todd S. Byars, Matt J. Griffin, David J. Wise","doi":"10.1002/naaq.10338","DOIUrl":"https://doi.org/10.1002/naaq.10338","url":null,"abstract":"ObjectiveThe Delta Select line of Channel Catfish <jats:italic>Ictalurus punctatus</jats:italic> was established in 2006 and has undergone five generations of genetic selection, specifically targeting increased growth rate and carcass yield, and was released to U.S. catfish farmers in 2020. While improvements in growth rate and carcass yield have been confirmed, performance for other important production traits has yet to be evaluated. Infectious disease, particularly bacterial infections caused by <jats:italic>Edwardsiella</jats:italic> spp., cause significant economic losses to catfish producers in the southeastern United States. Given the economic consequences of these bacterial pathogens, experimental infectivity trials were conducted comparing susceptibility to <jats:italic>Edwardsiella</jats:italic> spp. between the Delta Select line and a randomly bred line of Channel Catfish originating from the same base population (Delta Control line).MethodsTwenty‐six full‐sib families from each line were used in infectivity trials consisting of a high and a low dose of <jats:italic>E. ictaluri</jats:italic> (immersion challenge: high dose = 1.6 × 10<jats:sup>7</jats:sup> CFU/mL, low dose = 8.0 × 10<jats:sup>6</jats:sup> CFU/mL) and <jats:italic>E. piscicida</jats:italic> (intracoelomic challenge: high dose = 6.3 × 10<jats:sup>5</jats:sup> CFU/fish, low dose = 1.9 × 10<jats:sup>5</jats:sup> CFU/fish).ResultThe high challenge dose resulted in the highest mortality for both pathogens (<jats:italic>p</jats:italic> &lt; 0.001), regardless of selection line. There was no significant difference in cumulative percent mortality between the Delta Select and Delta Control lines following the <jats:italic>E. ictaluri</jats:italic> challenge at either dose (<jats:italic>p</jats:italic> = 0.55). Delta Selects had reduced mortality (<jats:italic>p</jats:italic> &lt; 0.001) in response to <jats:italic>E. piscicida</jats:italic> challenge compared with the Delta Controls, regardless of dose.ConclusionThese results indicate that selection for increased growth rate and carcass yield in the Delta Select Channel Catfish line did not negatively impact resistance to <jats:italic>E. ictaluri</jats:italic> while potentially improving resistance to <jats:italic>E. piscicida</jats:italic>.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140598172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacterial community of Parvocalanus crassirostris nauplii and the potential of nauplii as a vector for early probiotic supply to fish larvae 冠突伪尾柱虫稚鱼的细菌群落以及稚鱼作为向鱼类幼体提供早期益生菌的载体的潜力
IF 1 4区 农林科学
North American Journal of Aquaculture Pub Date : 2024-04-06 DOI: 10.1002/naaq.10331
Ulises Amador‐Marrero, Silvie Dumas, Sergio F. Martínez‐Díaz, Mauricio Contreras‐Olguín, Laura Flores‐Montijo, Bárbara González‐Acosta, Ricardo Vázquez‐Juárez, Norma Estrada, Patricia J. Ramírez‐Arenas
{"title":"Bacterial community of Parvocalanus crassirostris nauplii and the potential of nauplii as a vector for early probiotic supply to fish larvae","authors":"Ulises Amador‐Marrero, Silvie Dumas, Sergio F. Martínez‐Díaz, Mauricio Contreras‐Olguín, Laura Flores‐Montijo, Bárbara González‐Acosta, Ricardo Vázquez‐Juárez, Norma Estrada, Patricia J. Ramírez‐Arenas","doi":"10.1002/naaq.10331","DOIUrl":"https://doi.org/10.1002/naaq.10331","url":null,"abstract":"ObjectiveThe copepod <jats:italic>Parvocalanus crassirostris</jats:italic> has recently increased its use as live food in marine fish hatcheries.MethodsTo investigate the bacterial community of <jats:italic>P. crassirostris</jats:italic> nauplii and explore the potential use of nauplii as a vector for probiotics, adult copepods were reared with or without a probiotic consortium (<jats:italic>Bacillus</jats:italic> spp. and <jats:italic>Lactobacillus</jats:italic> spp.). Nauplii from both treatments were either enriched with probiotics or not, resulting in four treatments: control rearing and nonenriched, control rearing and probiotic enriched, probiotic rearing and nonenriched, and probiotic rearing probiotic enriched. A next‐generation sequencing analysis of the 16S ribosomal RNA gene was performed.ResultThe bacterial community of nauplii without probiotics was dominated by the phyla Proteobacteria (mean ± standard deviation = 51.5 ± 8.0%) and Bacteroidetes (27.6 ± 6.7%); from these phyla, the most abundant orders were Rhodobacterales (21.8 ± 2.0%), Flavobacteriales (13.5 ± 9.4%), Chitinophagales (9.7 ± 1.8%), Alteromonadales (9.5 ± 4.0%), and Burkholderiales (3.8 ± 0.8%). Opportunistic pathogenic bacterial genera, such as <jats:italic>Vibrio</jats:italic> spp. and <jats:italic>Pseudomonas</jats:italic> spp., were detected at very low relative abundances (&lt;1.2%), indicating that <jats:italic>P. crassirostris</jats:italic> nauplii could be considered a safe microbiological live feed. Furthermore, <jats:italic>P. crassirostris</jats:italic> nauplii were a good vector for probiotics delivery, as the adhesion of probiotics on the copepod exoskeleton was successful. <jats:italic>Bacillus</jats:italic> spp. abundance was 5–6× greater than the abundance of <jats:italic>Lactobacillus</jats:italic> spp. in <jats:italic>P. crassirostris</jats:italic> nauplii. The highest mean values of probiotics relative abundance (<jats:italic>Bacillus</jats:italic> spp. 41 ± 6.0%, <jats:italic>Lactobacillus</jats:italic> spp. 6.0 ± 3.0%) were observed in nauplii that were reared with probiotics and also enriched with probiotics.Conclusion<jats:italic>Parvocalanus crassirostris</jats:italic> nauplii stages I–II appear to be good vectors to provide probiotics to first‐feeding fish larvae.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140598165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Incubation of Walleye eggs in Petri dishes 在培养皿中孵化马口鱼卵
IF 1 4区 农林科学
North American Journal of Aquaculture Pub Date : 2024-03-20 DOI: 10.1002/naaq.10339
Jill M. Voorhees, Michael E. Barnes
{"title":"Incubation of Walleye eggs in Petri dishes","authors":"Jill M. Voorhees, Michael E. Barnes","doi":"10.1002/naaq.10339","DOIUrl":"https://doi.org/10.1002/naaq.10339","url":null,"abstract":"Eggs of Walleye Sander vitreus are typically incubated in upwelling jars. The relatively large number of eggs required for efficient and effective incubation in each jar, along with the difficulties in accurately quantifying egg survival to fry hatch, inhibits the replication needed for the controlled experimentation of Walleye spawning procedures and egg development. In addition, the use of production-sized incubators for experiments can be problematic because of the risks associated with potentially hazardous treatments on large numbers of eggs. This study evaluated the technique of incubating small numbers of Walleye eggs in Petri dishes.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140182076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Repeated copper sulfate treatments have no effect on growth and survival of juvenile Walleye 重复硫酸铜处理对幼年瓦勒耶鱼的生长和存活没有影响
IF 1 4区 农林科学
North American Journal of Aquaculture Pub Date : 2024-03-14 DOI: 10.1002/naaq.10333
Bradley D. Farmer, David L. Straus, J. Alan Johnson, Cynthia K. Ledbetter, Michael B. Deshotel
{"title":"Repeated copper sulfate treatments have no effect on growth and survival of juvenile Walleye","authors":"Bradley D. Farmer, David L. Straus, J. Alan Johnson, Cynthia K. Ledbetter, Michael B. Deshotel","doi":"10.1002/naaq.10333","DOIUrl":"https://doi.org/10.1002/naaq.10333","url":null,"abstract":"ObjectiveAn experiment was conducted to evaluate the effects of repeated copper sulfate (CuSO<jats:sub>4</jats:sub>) treatments on the growth and survival of juvenile Walleye <jats:italic>Sander vitreus</jats:italic>.MethodsCulture tanks were treated with 0 or 0.76 mg/L CuSO<jats:sub>4</jats:sub> every other day for five exposures, then were not treated for 14 consecutive days; this regimen was repeated for a total of 10 exposures over the course of 50 days. Copper sulfate treatments had a half‐life of about 60 min. The study was conducted in flow‐through well water at ≈1.5 L/m and 23.8°C.ResultThe resulting survival data for CuSO<jats:sub>4</jats:sub> treated and nontreated fish was 96% and 85% survival, respectively, and not statically different. There was no difference in weight, with treated weighing 25.3 g compared to 25.2 g for nontreated control fish. Both groups had weight increases of over 400% during the study. Neither group differed in length, with treated fish reaching a mean length of 14.6 cm and nontreated reaching a mean length of 14.5 cm.ConclusionThe results indicate that repeated CuSO<jats:sub>4</jats:sub> treatments had no effect on Walleye growth or survival.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140152658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Density affects rearing performance of juvenile landlocked fall Chinook Salmon 密度影响内陆秋季大鳞大麻哈鱼幼鱼的饲养性能
IF 1 4区 农林科学
North American Journal of Aquaculture Pub Date : 2024-03-11 DOI: 10.1002/naaq.10337
Alex J. Rosburg, Michael E. Barnes
{"title":"Density affects rearing performance of juvenile landlocked fall Chinook Salmon","authors":"Alex J. Rosburg, Michael E. Barnes","doi":"10.1002/naaq.10337","DOIUrl":"https://doi.org/10.1002/naaq.10337","url":null,"abstract":"Density-related information for landlocked fall Chinook Salmon <i>Oncorhynchus tshawytscha</i> shortly after first feeding is poorly understood. This study was undertaken to determine the effects of a range of rearing densities (low, intermediate, and high) on rearing performance and survival of newly feed-trained juvenile landlocked fall Chinook Salmon.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140097591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular mechanism of emodin in inhibiting the activity of Aeromonas hydrophila via AtpE 大黄素通过 AtpE 抑制嗜水气单胞菌活性的分子机制
IF 1 4区 农林科学
North American Journal of Aquaculture Pub Date : 2024-02-21 DOI: 10.1002/naaq.10330
Huimin Zhang, Jun Zhou, Xuguang Li, Yanfei Deng, Xianping Ge, Bo Liu
{"title":"Molecular mechanism of emodin in inhibiting the activity of Aeromonas hydrophila via AtpE","authors":"Huimin Zhang, Jun Zhou, Xuguang Li, Yanfei Deng, Xianping Ge, Bo Liu","doi":"10.1002/naaq.10330","DOIUrl":"https://doi.org/10.1002/naaq.10330","url":null,"abstract":"Objective<jats:italic>Aeromonas hydrophila</jats:italic>, a leading pathogen of aquatic organisms, poses a significant challenge in terms of effective and safe inhibition.MethodsThrough the method of experiments and molecular simulations, we discovered that emodin effectively inhibits the <jats:italic>A. hydrophila</jats:italic> via the binding of emodin with AtpE (ATP synthase subunit C). Bacterial inhibition experiments show that emodin effectively inhibits the activity and growth of <jats:italic>A. hydrophila</jats:italic>.ResultFurther molecular docking and molecular dynamics simulation revealed that emodin binds directly to AtpE. More specifically, it binds to certain residues of AtpE, including TYR73, LEU70, ALA13, TYR10, MET17, and ALA14, ultimately leading to inhibition of activity.ConclusionConsequently, our discovery sheds new light on exploring the molecular mechanism through which emodin displays antimicrobial activity against <jats:italic>A. hydrophila</jats:italic>.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139954755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Initial investigations into the production of triploid sunshine bass using temperature shock 利用温度冲击生产三倍体阳光鲈鱼的初步研究
IF 1 4区 农林科学
North American Journal of Aquaculture Pub Date : 2024-02-09 DOI: 10.1002/naaq.10329
David L. Straus, Jason W. Abernathy, Anita M. Kelly, Herbert E. Quintero, T. Mike Freeze, Ric S. Williams
{"title":"Initial investigations into the production of triploid sunshine bass using temperature shock","authors":"David L. Straus, Jason W. Abernathy, Anita M. Kelly, Herbert E. Quintero, T. Mike Freeze, Ric S. Williams","doi":"10.1002/naaq.10329","DOIUrl":"https://doi.org/10.1002/naaq.10329","url":null,"abstract":"The sunshine bass (female White Bass <i>Morone chrysops</i> × male Striped Bass <i>M. saxatilis</i>) is an important food fish raised in U.S. aquaculture. Spawning is performed by manually stripping and fertilizing White Bass eggs with Striped Bass sperm. Embryos hatch in approximately 48 h. Mature sunshine bass females can become fertile and produce viable eggs, which is problematic in commercial food-fish production; therefore, triploid fish are desired.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139769159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Using digestive physiology to develop larval culture protocols for the Blackbanded Sunfish, a threatened centrarchid native to the United States 利用消化生理学制定黑带太阳鱼(一种原产于美国的濒危中心鱼类)的幼虫培养方案
IF 1 4区 农林科学
North American Journal of Aquaculture Pub Date : 2024-01-25 DOI: 10.1002/naaq.10328
Casey A. Murray, Taylor N. Lipscomb, Amy L. Wood, Quenton M. Tuckett, Josh T. Patterson, Craig A. Watson, Matthew A. DiMaggio
{"title":"Using digestive physiology to develop larval culture protocols for the Blackbanded Sunfish, a threatened centrarchid native to the United States","authors":"Casey A. Murray, Taylor N. Lipscomb, Amy L. Wood, Quenton M. Tuckett, Josh T. Patterson, Craig A. Watson, Matthew A. DiMaggio","doi":"10.1002/naaq.10328","DOIUrl":"https://doi.org/10.1002/naaq.10328","url":null,"abstract":"The Blackbanded Sunfish <i>Enneacanthus chaetodon</i> is a small centrarchid that is endemic to slow-moving waters from New Jersey to central Florida, United States. The distribution of this species has been increasingly fragmented, primarily due to habitat degradation. The development of larval culture protocols is essential to produce this species for the aquarium trade and for potential restoration efforts. To streamline the production process, larvae should be transitioned from live feeds to inert diets as early as possible without sacrificing larval growth or survival.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139583899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of in vitro and in vivo digestibility of potential feed ingredients for juvenile Yellowtail Snapper 评估黄尾鲷幼鱼潜在饲料成分的体外和体内消化率
IF 1 4区 农林科学
North American Journal of Aquaculture Pub Date : 2024-01-20 DOI: 10.1002/naaq.10316
Alvaro Barreto, Martín Arenas, Alfonso Álvarez-González, Jaime Suárez-Bautista, Adolfo Sánchez, Carlos Maldonado, Gerard Cuzon, Gabriela Gaxiola
{"title":"Evaluation of in vitro and in vivo digestibility of potential feed ingredients for juvenile Yellowtail Snapper","authors":"Alvaro Barreto, Martín Arenas, Alfonso Álvarez-González, Jaime Suárez-Bautista, Adolfo Sánchez, Carlos Maldonado, Gerard Cuzon, Gabriela Gaxiola","doi":"10.1002/naaq.10316","DOIUrl":"https://doi.org/10.1002/naaq.10316","url":null,"abstract":"The carnivorous Yellowtail Snapper <i>Ocyurus chrysurus</i> has the potential for production in aquaculture. However, there is a lack of studies investigating the digestibility of ingredients needed to formulate balanced diets for this species.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139556471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nutritional value of torula yeast as an alternative protein feedstuff for hybrid Striped Bass (White Bass × Striped Bass) 鱼腥草酵母作为杂交条纹鲈(白鲈×条纹鲈)替代蛋白饲料的营养价值
IF 1 4区 农林科学
North American Journal of Aquaculture Pub Date : 2023-12-25 DOI: 10.1002/naaq.10313
Kequan Chen, Fernando Y. Yamamoto, Clement R. de Cruz, Delbert M. Gatlin
{"title":"Nutritional value of torula yeast as an alternative protein feedstuff for hybrid Striped Bass (White Bass × Striped Bass)","authors":"Kequan Chen, Fernando Y. Yamamoto, Clement R. de Cruz, Delbert M. Gatlin","doi":"10.1002/naaq.10313","DOIUrl":"https://doi.org/10.1002/naaq.10313","url":null,"abstract":"Two separate trials were conducted to evaluate the digestibility of a novel alternative ingredient, enhanced torula yeast (TY) <i>Cyberlindnera jadinii</i>, in diets for hybrid Striped Bass (HSB; White Bass <i>Morone chrysops</i> × Striped Bass <i>M. saxatilis</i>) and to evaluate graded inclusion levels of this product when replacing soybean products.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139036013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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