Nova Biotechnologica et Chimica最新文献_第2页

Immobilization of beta-glucosidase purified from mandarin (Citrus reticulata) fruit to superparamagnetic nanoparticles and its aroma quality enhancing effect 超顺磁性纳米粒子固定化柑桔β-葡萄糖苷酶及其增香作用

Nova Biotechnologica et Chimica Pub Date : 2023-07-21 DOI: 10.34135/nbc.1452

M. Acar, Y. Turan, Olcay Sinan, S. Sinan

{"title":"Immobilization of beta-glucosidase purified from mandarin (Citrus reticulata) fruit to superparamagnetic nanoparticles and its aroma quality enhancing effect","authors":"M. Acar, Y. Turan, Olcay Sinan, S. Sinan","doi":"10.34135/nbc.1452","DOIUrl":"https://doi.org/10.34135/nbc.1452","url":null,"abstract":"This paper reports on novel and efficient enhancement effects of fruit juice aroma using immobilized β-glucosidase, the enzyme involved in important functions in living organisms, onto superparamagnetic nanoparticles Fe3O4 via carbodiimide β-glucosidase was purified from mandarin (Citrus reticulata) using ammonium sulfate precipitation and hydrophobic interaction chromatography. To be used in this study, superparamagnetic nanoparticles were synthesized and then the shape, size, and magnetism properties of the nanoparticles were characterized. The purified enzyme was immobilized on the nanoparticles. The optimum temperature for β-glucosidase (40 ℃) was increased by 10 ℃ after immobilization, while the optimum pH values of free and immobilized β-glucosidase were 5.5. While the Km and Vmax values of the free enzyme were 0.264 mM and 294 EU, immobilized enzyme’s Km and Vmax were 0.222 mM and 370 EU, respectively. In addition, it was determined that the storage stability of the immobilized enzyme was higher than the free enzyme. When the effect of some metal ions on the enzyme activity was examined, it was observed that Fe+2 increased the enzyme activity while other metals inhibited it. According to the results obtained, the immobilized enzyme had a flavor-enhancing effect on mandarin juice.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44744556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive molecules from Algerian propolis with therapeutic potential against the oral pathogen Enterococcus faecalis and Chromobacterium violaceum CV026 阿尔及利亚蜂胶的生物活性分子对口腔病原体粪肠球菌和紫曲霉CV026具有治疗潜力

Nova Biotechnologica et Chimica Pub Date : 2023-07-21 DOI: 10.34135/nbc.1599

Meryem Mokrani, A. Zellagui, Widad Hadjab, M. Öztürk, Ozgur Ceylen, C. Bensouici

{"title":"Bioactive molecules from Algerian propolis with therapeutic potential against the oral pathogen Enterococcus faecalis and Chromobacterium violaceum CV026","authors":"Meryem Mokrani, A. Zellagui, Widad Hadjab, M. Öztürk, Ozgur Ceylen, C. Bensouici","doi":"10.34135/nbc.1599","DOIUrl":"https://doi.org/10.34135/nbc.1599","url":null,"abstract":"This research aims to analyse the richness of propolis ethanolic extract from Kherrata (EEPKh) in phenolic and flavonoid compounds and its antioxidant effects using different methods. Further investigations were conducted to evaluate the antibacterial and antibiofilm potential of propolis against Enterococcus faecalis strains originated from oral diseases. The antiquorum-sensing ability against Chromobacterium violaceum CV 026 was also investigated. The results revealed that ethanolic extract contains a high content of phenolic and flavonoid compounds, with an amount of 734.3 9± 11.54µg GAE.mg-1 of Extract and 224.30 ± 0µg QE.mg-1of extract respectively. Caffeic acid (23.79 mg.g-1), hesperetin (15.42 mg.g-1), cynarin (7.59 mg.g-1), apigenin (5.91 mg.g-1), naringenin (4.90 mg.g-1), and kaempferol (3.43 mg.g-1) were identified as the major compounds by the HPLC-DAD analysis. The antioxidant activity showed good scavenging and reducing abilities. Furthermore, EEPKh demonstrated high antibacterial potency against E. faecalis strain 2 at concentration 20 mg.mL-1 with an inhibition diameter of 20.33 ± 0.57 mm. The MIC and MBC values were found to range between 0.625 and 10 mg.mL-1. Biofilm formation by E. faecalis strains was inhibited at MIC with a percentage ranging from 65.93 ± 1.11 to 51.54 ± 0.81%. Quorum sensing mechanisms in CV 026 was inhibited by EEPKh, with diameter zone of 11.16 ± 0.29 mm at MIC. This study indicated that propolis extract is considered as a new source of natural medication with therapeutic potential against oral pathology caused by free radicals, E. faecalis, biofilm formation and quorum-sensing.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45616701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced vitamin B12 production using paneer whey as culture medium 使用帕尼尔乳清作为培养基提高维生素B12的生产

Nova Biotechnologica et Chimica Pub Date : 2023-02-03 DOI: 10.34135/nbc.1339

Sangavi Gopalakrishnan, Harini Rajkumar, U. Antony, Lakshmi B.S., N. Ganesan

引用次数: 0

Antibiofilm activities of crude extract and supernatant of Actinobacteria against biofilm formed by foodborne pathogens and food spoilage bacteria 放线菌粗提物和上清液对食源性致病菌和食品腐败菌形成的生物膜的抗菌活性

Nova Biotechnologica et Chimica Pub Date : 2023-02-03 DOI: 10.34135/nbc.1283

Faustina Audrey Agatha, Diana Elizabeth Waturangi

{"title":"Antibiofilm activities of crude extract and supernatant of Actinobacteria against biofilm formed by foodborne pathogens and food spoilage bacteria","authors":"Faustina Audrey Agatha, Diana Elizabeth Waturangi","doi":"10.34135/nbc.1283","DOIUrl":"https://doi.org/10.34135/nbc.1283","url":null,"abstract":"Bacteria form a biofilm that attaches the microbial population to a solid surface, thus acting as a barrier to protect external stresses from the bacterial community. In the food industry, biofilms are believed to be responsible for food-borne diseases and food spoilages. This research was conducted to characterize anti-quorum sensing (QS), anti-biofilm activity of crude extract, and supernatant from Actinobacteria isolates against biofilm of foodborne, and food spoilage bacteria. Actinobacteria is the group of filamentous spore-forming Gram-positive bacteria, which is well recognized as a source for novel secondary metabolites, such as anti-biofilm and anti-quorum sensing. Two isolates of Actinobacteria utilized in this study were recovered from the Indonesian marine environment in the previous study. Both isolates demonstrated anti-QS activity on early detection assay using the agar overlay method. The Actinobacteria isolates were fermented, and the crude extracts were obtained through extraction using 100 % ethyl acetate and further evaporation, while the supernatant was collected directly following fermentation. The entire Actinobacteria isolates displayed capability as an anti-biofilm agent in crude extracts form at a concentration of 20 mg.mL-1, alongside when in its supernatant form. The Actinobacteria isolates in both extracts and supernatants form demonstrated anti-QS activity based on the N-Hexanoyl-L- homoserine lactone (HHL) validation assay and were also categorized as non-toxic based on Brine Shrimp Lethality Assay (BSLA).","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135206991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Health risks associated with the chronic consumption of energy drinks in children and adolescents 儿童和青少年长期饮用能量饮料的健康风险

Nova Biotechnologica et Chimica Pub Date : 2023-02-03 DOI: 10.34135/nbc.1344

K. Mphahlele, N. Ngqwala, Oscar Agbor Ambang, C. S. Srinivas, R. Tandlich

{"title":"Health risks associated with the chronic consumption of energy drinks in children and adolescents","authors":"K. Mphahlele, N. Ngqwala, Oscar Agbor Ambang, C. S. Srinivas, R. Tandlich","doi":"10.34135/nbc.1344","DOIUrl":"https://doi.org/10.34135/nbc.1344","url":null,"abstract":"Energy drinks (EDs) have been available at the global market for almost 100 years and today, they are advertised as ’tools’ boost energy, enhance physical performance and mental alertness. Some of the main chemical components in EDs include caffeine, ginseng, and taurine. The market and consumption of these beverages is growing exponentially, and this is becoming a public health problem due to the adverse effects associated with these drinks. The main objective of this article is to review important ingredients in popular EDs in South Africa and look at their molecular mechanisms of action and interaction with other compounds within the body. At the same time, the authors aim to review the global consumption pattern of EDs among children and adolescents. Finally, this review article will provide an overview the health risks associated with EDs consumption. A literature review was conducted using scholarly databases. Keywords such as energy drinks, adverse effects, advertising, alcohol, caffeine, taurine, and regulations were used. Despite the claims of having significant benefits to mental and physical stamina, long-term consumption of EDs could have detrimental public health implications; and could result in increasing rates of the central nervous system disorders and the cardiovascular complications. A knowledge gap exists on how the lack of education impacts the decision of consumers of EDs and the parental guidance provided to children and adolescents in relation to the consumption of these beverages in South Africa. To address the issue of excessive ED consumption, communities need to be made aware of the harmful effects of these beverages through health education and implementing strict regulatory measures through policy.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43988759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the inhibitory effect of Lactobacillus strains and lysozyme on growth of Clostridium spp. responsible for cheese late blowing defect 乳酸杆菌和溶菌酶对干酪后期发泡缺陷梭菌生长抑制作用的研究

Nova Biotechnologica et Chimica Pub Date : 2022-12-07 DOI: 10.34135/nbc.1229

Jorge Olivera Rodi, Marcela Joan Gonz´ález Ramos, Pedro Díaz Gadea, Stella M. Reginensi

引用次数: 0

Antibiofilm activities of crude extract and supernatant of Actinobacteria against biofilm formed by foodborne pathogens and food spoilage bacteria 放线菌粗提物和上清液对食源性致病菌和食品腐败菌形成的生物膜的抗菌活性

Nova Biotechnologica et Chimica Pub Date : 2022-12-06 DOI: 10.36547/nbc.1283

F. Agatha, D. Waturangi

{"title":"Antibiofilm activities of crude extract and supernatant of Actinobacteria against biofilm formed by foodborne pathogens and food spoilage bacteria","authors":"F. Agatha, D. Waturangi","doi":"10.36547/nbc.1283","DOIUrl":"https://doi.org/10.36547/nbc.1283","url":null,"abstract":"Bacteria form a biofilm that attaches the microbial population to a solid surface, thus acting as a barrier to protect external stresses from the bacterial community. In the food industry, biofilms are believed to be responsible for food-borne diseases and food spoilages. This research was conducted to characterize anti-quorum sensing (QS), anti-biofilm activity of crude extract, and supernatant from Actinobacteria isolates against biofilm of foodborne, and food spoilage bacteria. Actinobacteria is the group of filamentous spore-forming Gram-positive bacteria, which is well recognized as a source for novel secondary metabolites, such as anti-biofilm and anti-quorum sensing. Two isolates of Actinobacteria utilized in this study were recovered from the Indonesian marine environment in the previous study. Both isolates demonstrated anti-QS activity on early detection assay using the agar overlay method. The Actinobacteria isolates were fermented, and the crude extracts were obtained through extraction using 100 % ethyl acetate and further evaporation, while the supernatant was collected directly following fermentation. The entire Actinobacteria isolates displayed capability as an anti-biofilm agent in crude extracts form at a concentration of 20 mg.mL-1, alongside when in its supernatant form. The Actinobacteria isolates in both extracts and supernatants form demonstrated anti-QS activity based on the N-Hexanoyl-L- homoserine lactone (HHL) validation assay and were also categorized as non-toxic based on Brine Shrimp Lethality Assay (BSLA).","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42363794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of chromatographic performance of three C18 columns for avenanthramides separation 三种C18柱分离燕麦蒽酰胺的色谱性能评价

Nova Biotechnologica et Chimica Pub Date : 2022-11-03 DOI: 10.36547/nbc.1483

Timotej Jankech, J. Sokol, M. Maliarová, Ivana Gerhardtová, Nicholas Martinka, Marcela Blažková, M. Havrlentová

{"title":"Evaluation of chromatographic performance of three C18 columns for avenanthramides separation","authors":"Timotej Jankech, J. Sokol, M. Maliarová, Ivana Gerhardtová, Nicholas Martinka, Marcela Blažková, M. Havrlentová","doi":"10.36547/nbc.1483","DOIUrl":"https://doi.org/10.36547/nbc.1483","url":null,"abstract":"Phenolic amides contained in oats (Avenanthramides; AVNs) are biologically active substances with strong antioxidant activity. In this paper, we evaluated efficiency of three C18 chromatographic columns (Symmetry, XBridge, Cortecs) with different particle technology and particle sizes for the separation of three major avenanthramides (AVN A, AVN B, AVN C). We compared columns in terms of retention times, retention factors of AVNs and in terms of other parameters such as number of theoretical plates, height equivalent to a theoretical plate, reduced plate height, resolution and in terms of peak symmetry, respectively. Limits of detection and limits of quantification of AVNs on all columns were calculated. Retention results of AVNs on individual columns showed a significant reduction in retention times of AVNs on solid core column with a particle size 2.7 μm compared to columns with particle size 3.5 μm. Within columns with 3.5 μm particles, separation on Symmetry C18 column appeared to be more efficient than on the XBridge C18 column. In general, results achieved on Cortecs C18 column can be considered as the best in terms of both separation efficiency and retention times.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45032335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New approaches in pretreatment methods of beech particles in enzymatic hydrolysis – effect of impregnation time and reactor selection 酶解山毛榉颗粒预处理方法的新探索——浸渍时间和反应器选择的影响

Nova Biotechnologica et Chimica Pub Date : 2022-08-24 DOI: 10.36547/nbc.1247

A. Pažitný, A. Russ, Š. Boháček, M. Halaj, Š. Šutý, Ida Skotnicová, V. Ihnát

{"title":"New approaches in pretreatment methods of beech particles in enzymatic hydrolysis – effect of impregnation time and reactor selection","authors":"A. Pažitný, A. Russ, Š. Boháček, M. Halaj, Š. Šutý, Ida Skotnicová, V. Ihnát","doi":"10.36547/nbc.1247","DOIUrl":"https://doi.org/10.36547/nbc.1247","url":null,"abstract":"Waste dendromass is one of the least used types of biomass and together with waste from the harvesting and processing of agricultural crops, belongs to the wastes that have a high potential for the second-generation ethanol production. Mass concentration of biofuel added to fossil fuels is established by law and this concentration is determined within the reference values for each year. For example, for 2021 it was 8.0 % and for years from 2022 to 2030 the value will increase to 8.2 %. Biofuels in the so-called reference value in the total fuel content have been mandatory since 2017, while the specific reference value is calculated from the energy content of the total amount of fuels placed on the market. This paper provides initial considerations and pilot results based on experimental data obtained in our workplace concerning the application potential of selected type of dendromass – beech particles from the point of view of their pretreatment by steam explosion and steam extrusion for potential production of liquid biofuels based on lignocellulosic biomass. We compared temperature courses of the mentioned pretreatment methods and pretreated samples were tested by enzymatic hydrolysis. Enzymatic hydrolysis results showed that steam explosion and steam extrusion led to concentration enhancement of fermentable monosaccharides compared to original samples depending on used pretreatment method.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42726393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical profiles and antibacterial activities of acetone extracts of Globba macrocarpa 大果球丙酮提取物的化学成分及抗菌活性

Nova Biotechnologica et Chimica Pub Date : 2022-08-17 DOI: 10.36547/nbc.1322

Hong Thien Van, Tan Viet Pham, Ngoc An Nguyen, Hanh Thi Dieu Nguyen, Quoc Hung Nguyen, Thanh Tho Le, Van Son Le

{"title":"Chemical profiles and antibacterial activities of acetone extracts of Globba macrocarpa","authors":"Hong Thien Van, Tan Viet Pham, Ngoc An Nguyen, Hanh Thi Dieu Nguyen, Quoc Hung Nguyen, Thanh Tho Le, Van Son Le","doi":"10.36547/nbc.1322","DOIUrl":"https://doi.org/10.36547/nbc.1322","url":null,"abstract":"Globba macrocarpa Gagnep. is a rare species of Globba genus (Zingiberaceae family). The present study reported the chemical compositions and antibacterial effects of acetone extracts obtained from the G. macrocarpa rhizomes and aerial parts. By using Gas Chromatography Mass Spectrometry (GC/MS) assay, fifty and thirty-two chemical compounds were identified from rhizomes and aerial parts of the species, respectively. Of those, germacrene D (15.25 %), 1H-indole, 4-(3-methyl-2-butenyl)- (14.33 %), (E)-β-farnesene (11.28 %), and 2-biphenylamine, 3-methyl (10.27 %) were the major constituents in the rhizome extract while the aerial part extract was characterized by the predominance of linolenic acid (19.89 %), palmitic acid (13.05 %), phytol (7.52 %), and neophytadiene (4.76 %). In addition, the rhizome extract had antibacterial activities against five 5 out of 6 oral bacteria, including Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium Bacillus cereus, and Staphylococcus aureus. Meanwhile, the aerial part extract was active against 4 out of 6 test bacteria, except for Escherichia coli and S. typhimurium.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49237327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0