Nova Biotechnologica et Chimica最新文献_第2页

Evaluation of chromatographic performance of three C18 columns for avenanthramides separation 三种C18柱分离燕麦蒽酰胺的色谱性能评价

Nova Biotechnologica et Chimica Pub Date : 2022-11-03 DOI: 10.36547/nbc.1483

Timotej Jankech, J. Sokol, M. Maliarová, Ivana Gerhardtová, Nicholas Martinka, Marcela Blažková, M. Havrlentová

{"title":"Evaluation of chromatographic performance of three C18 columns for avenanthramides separation","authors":"Timotej Jankech, J. Sokol, M. Maliarová, Ivana Gerhardtová, Nicholas Martinka, Marcela Blažková, M. Havrlentová","doi":"10.36547/nbc.1483","DOIUrl":"https://doi.org/10.36547/nbc.1483","url":null,"abstract":"Phenolic amides contained in oats (Avenanthramides; AVNs) are biologically active substances with strong antioxidant activity. In this paper, we evaluated efficiency of three C18 chromatographic columns (Symmetry, XBridge, Cortecs) with different particle technology and particle sizes for the separation of three major avenanthramides (AVN A, AVN B, AVN C). We compared columns in terms of retention times, retention factors of AVNs and in terms of other parameters such as number of theoretical plates, height equivalent to a theoretical plate, reduced plate height, resolution and in terms of peak symmetry, respectively. Limits of detection and limits of quantification of AVNs on all columns were calculated. Retention results of AVNs on individual columns showed a significant reduction in retention times of AVNs on solid core column with a particle size 2.7 μm compared to columns with particle size 3.5 μm. Within columns with 3.5 μm particles, separation on Symmetry C18 column appeared to be more efficient than on the XBridge C18 column. In general, results achieved on Cortecs C18 column can be considered as the best in terms of both separation efficiency and retention times.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45032335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New approaches in pretreatment methods of beech particles in enzymatic hydrolysis – effect of impregnation time and reactor selection 酶解山毛榉颗粒预处理方法的新探索——浸渍时间和反应器选择的影响

Nova Biotechnologica et Chimica Pub Date : 2022-08-24 DOI: 10.36547/nbc.1247

A. Pažitný, A. Russ, Š. Boháček, M. Halaj, Š. Šutý, Ida Skotnicová, V. Ihnát

{"title":"New approaches in pretreatment methods of beech particles in enzymatic hydrolysis – effect of impregnation time and reactor selection","authors":"A. Pažitný, A. Russ, Š. Boháček, M. Halaj, Š. Šutý, Ida Skotnicová, V. Ihnát","doi":"10.36547/nbc.1247","DOIUrl":"https://doi.org/10.36547/nbc.1247","url":null,"abstract":"Waste dendromass is one of the least used types of biomass and together with waste from the harvesting and processing of agricultural crops, belongs to the wastes that have a high potential for the second-generation ethanol production. Mass concentration of biofuel added to fossil fuels is established by law and this concentration is determined within the reference values for each year. For example, for 2021 it was 8.0 % and for years from 2022 to 2030 the value will increase to 8.2 %. Biofuels in the so-called reference value in the total fuel content have been mandatory since 2017, while the specific reference value is calculated from the energy content of the total amount of fuels placed on the market. This paper provides initial considerations and pilot results based on experimental data obtained in our workplace concerning the application potential of selected type of dendromass – beech particles from the point of view of their pretreatment by steam explosion and steam extrusion for potential production of liquid biofuels based on lignocellulosic biomass. We compared temperature courses of the mentioned pretreatment methods and pretreated samples were tested by enzymatic hydrolysis. Enzymatic hydrolysis results showed that steam explosion and steam extrusion led to concentration enhancement of fermentable monosaccharides compared to original samples depending on used pretreatment method.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42726393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical profiles and antibacterial activities of acetone extracts of Globba macrocarpa 大果球丙酮提取物的化学成分及抗菌活性

Nova Biotechnologica et Chimica Pub Date : 2022-08-17 DOI: 10.36547/nbc.1322

Hong Thien Van, Tan Viet Pham, Ngoc An Nguyen, Hanh Thi Dieu Nguyen, Quoc Hung Nguyen, Thanh Tho Le, Van Son Le

{"title":"Chemical profiles and antibacterial activities of acetone extracts of Globba macrocarpa","authors":"Hong Thien Van, Tan Viet Pham, Ngoc An Nguyen, Hanh Thi Dieu Nguyen, Quoc Hung Nguyen, Thanh Tho Le, Van Son Le","doi":"10.36547/nbc.1322","DOIUrl":"https://doi.org/10.36547/nbc.1322","url":null,"abstract":"Globba macrocarpa Gagnep. is a rare species of Globba genus (Zingiberaceae family). The present study reported the chemical compositions and antibacterial effects of acetone extracts obtained from the G. macrocarpa rhizomes and aerial parts. By using Gas Chromatography Mass Spectrometry (GC/MS) assay, fifty and thirty-two chemical compounds were identified from rhizomes and aerial parts of the species, respectively. Of those, germacrene D (15.25 %), 1H-indole, 4-(3-methyl-2-butenyl)- (14.33 %), (E)-β-farnesene (11.28 %), and 2-biphenylamine, 3-methyl (10.27 %) were the major constituents in the rhizome extract while the aerial part extract was characterized by the predominance of linolenic acid (19.89 %), palmitic acid (13.05 %), phytol (7.52 %), and neophytadiene (4.76 %). In addition, the rhizome extract had antibacterial activities against five 5 out of 6 oral bacteria, including Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium Bacillus cereus, and Staphylococcus aureus. Meanwhile, the aerial part extract was active against 4 out of 6 test bacteria, except for Escherichia coli and S. typhimurium.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49237327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial activity of medicinal plants against Streptococcus agalactiae 药用植物对无乳链球菌的抗菌活性

Nova Biotechnologica et Chimica Pub Date : 2022-07-19 DOI: 10.36547/nbc.1264

M. Novosak, D. L. Winnik, Margarita Ester Laczeski, M. Quiroga

{"title":"Antibacterial activity of medicinal plants against Streptococcus agalactiae","authors":"M. Novosak, D. L. Winnik, Margarita Ester Laczeski, M. Quiroga","doi":"10.36547/nbc.1264","DOIUrl":"https://doi.org/10.36547/nbc.1264","url":null,"abstract":"Streptococcus agalactiae, group B Streptococcus (GBS), infects and causes severe diseases in humans and numerous animal species, including fish, given its ability to cross the host-specific barrier. The emergence of antibiotic resistant GBS strains makes it necessary to look for alternatives to treat and prevent infections that it produces. The aim of the present study was to determine the antibacterial activity of ethanolic and aqueous extracts of medicinal plants from Misiones province, northeast Argentina, against GBS from humans and fish. We used human Streptococcus agalactiae ATCC® BAA-611™ and tilapia Streptococcus agalactiae ATCC® 51487™ strains. Minimum Inhibitory Dose (MID) was determined by the disc diffusion method. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), MBC/MIC ratio, drug synergism with commercial antibiotics, and resistance assays were determined with extracts that showed antibacterial activity. Medium Lethal Dose 50 (LD50) was determined by the Artemia salina assay. For ethanolic and aqueous Eugenia uniflora L. extracts, we obtained a MID = 0.5 mg.disc-1. For both extracts of Eugenia uniflora L., the MIC and MBC values were 1 mg.mL-1 and 5 mg.mL-1, respectively. The MICI (MBC/MIC ratio) = 5 qualified the action of these extracts as bacteriostatic. The drug synergism assay with ampicillin, erythromycin, and clindamycin combination and extracts showed indifference. The LD50 of the aqueous extract was 0.82 mg.mL-1 indicating moderate toxicity. This work is a first step to identify chemical compounds in native medicinal plants of Misiones, Argentina, that could mean an alternative for the treatment of Streptococcus agalactiae infections.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49666688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of functional effects of different LAB isolated from sourdoughs in Turkey 从土耳其酸面团中分离的不同LAB的功能效应表征

Nova Biotechnologica et Chimica Pub Date : 2022-07-18 DOI: 10.36547/nbc.1155

Z. Alkay, Ezgi Metin Sagir, Muhammed Ozgolet, M. Durak

{"title":"Characterization of functional effects of different LAB isolated from sourdoughs in Turkey","authors":"Z. Alkay, Ezgi Metin Sagir, Muhammed Ozgolet, M. Durak","doi":"10.36547/nbc.1155","DOIUrl":"https://doi.org/10.36547/nbc.1155","url":null,"abstract":"In this study, the technological properties of five different lactic acid bacteria (LAB) isolated from sourdough collected from three cities of Turkey (Gümüşhane, Manisa, Ankara) and Cyprus were investigated. For this purpose, antimicrobial, antifungal, phytase, and proteolytic activities of these bacteria and their effect on pH were examined. The pH of the prepared solutions decreased to 3.8 and 4.4 from 6.3 by LAB addition following 24 h incubation. Lactiplantibacillus plantarum O6F-25 strain showed the best inhibitory effect against four gram-positive and four gram-negative bacteria. In terms of proteolytic activity, Lactiplantibacillus plantarum 45MK-32 was the most effective strain. The antifungal effects of LAB were tested against Aspergillus flavus, Aspergillus niger, and Penicillium carneum. Levilactobacillus brevis KCO-48 was the most effective LAB strain. Phytase activities (710.40 – 840.37 U.mL-1) of LAB studied except Limosilactobacillus fermentum (29GT-19), which has the lowest phytase activity, were not significantly different (P < 0.05). This study revealed that sourdough LABs have the potential to be used as biopreservative and produce functional food products.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42884172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic, genotypic, and technological characterization of new lactic acid bacterium isolated from traditional dairy product in Djelfa, Algeria 阿尔及利亚杰尔法传统乳制品中分离的新型乳酸菌的表型、基因型和技术特征

Nova Biotechnologica et Chimica Pub Date : 2022-07-15 DOI: 10.36547/nbc.904

G. Mourad, Omrane Toumatia, S. Medjekal, B. Guessas, S. Cheriet, N. Bouras

引用次数: 0

Phenolic contents, antioxidant, and enzyme inhibitory effects of crude extracts of Polycarpon polycarpoides Fiori subsp. catalaunicum O. Bolòs & Vigo 聚卡pon多萜Fiori亚种粗提物的酚含量、抗氧化和酶抑制作用。catalaunicum O.Bolås&Vigo

Nova Biotechnologica et Chimica Pub Date : 2022-07-14 DOI: 10.36547/nbc.1228

Madiha Arioua, Asma Cheribet, Lynda Gali

{"title":"Phenolic contents, antioxidant, and enzyme inhibitory effects of crude extracts of Polycarpon polycarpoides Fiori subsp. catalaunicum O. Bolòs & Vigo","authors":"Madiha Arioua, Asma Cheribet, Lynda Gali","doi":"10.36547/nbc.1228","DOIUrl":"https://doi.org/10.36547/nbc.1228","url":null,"abstract":"The present work aimed to study some pharmacological effects of extracts of the aerial parts of Polycarpon polycarpoides Fiori subsp. catalaunicum O. Bolòs & Vigo (Caryophyllaceae) widely distributed in the Mediterranean basin. The crude extracts were prepared using four solvents (petroleum ether, ethyl acetate, methanol, and methanol: water 1 : 1, v/v), and then examined for their phenolic contents using spectrophotometric methods and for their in vitro antioxidant activity and ability to inhibit urease, acetylcholinesterase, and α-amylase. Low levels of total phenolic were recorded ranging from 14.39 ± 3.40 to 101.84 μg.GAE.mg-1 of extract corresponding to the petroleum ether and ethyl acetate extracts, respectively. As for the flavonoids, very low values varying from 0.37 ± 0.13 to 4.22 ± 0.83 μg.GAE.mg-1 of extract were obtained. A moderate antioxidant effect was exerted by the extracts most often the methanol and ethyl acetate extracts were the most potent probably due to their polyphenolic content. Remarkable inhibitory effect has been exhibited by the extracts against α-amylase and more specifically, the petroleum ether extract displayed the strongest capacity with a percentage inhibition of 48.19 ± 2.99 % at 400 µg.mL-1. However, all extracts were inactive against urease and acetylcholinesterase. These results could constitute a starting point for carrying out more studies on the plant in order to assess the possibility of valuing it as a source of bioactive compounds.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45265953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optimization of procedural factors for advanced xylanase synthesis by Lysinibacillus fusiformis using Kolanut husk as substrate 以科拉努特壳为底物的梭形赖氨菌合成高级木聚糖酶工艺条件的优化

Nova Biotechnologica et Chimica Pub Date : 2022-07-14 DOI: 10.36547/nbc.1226

Suliat Olatidayo Omisore, D. Arotupin, M. Bayode

{"title":"Optimization of procedural factors for advanced xylanase synthesis by Lysinibacillus fusiformis using Kolanut husk as substrate","authors":"Suliat Olatidayo Omisore, D. Arotupin, M. Bayode","doi":"10.36547/nbc.1226","DOIUrl":"https://doi.org/10.36547/nbc.1226","url":null,"abstract":"Xylan is a complex hetero-polysaccharide consisting of different monosaccharides held together by glycosidic and ester bonds. Extracellular xylanase fashioned by numerous microbes principally from bacterial species such as Bacillus species are responsible for cleaving the glycosidic linkages. Microbial xylanases exhibit different substrate specificities and biochemical peculiarities. This study was carried out for optimization of cultivation conditions for xylanase production using the bacterium Lysinibacillus fusiformis and Kolanut husk as a component of cultivation medium. The bacterium was isolated from Kolanut plantation waste soil and screened for the production of xylanase qualitatively on xylan nutrient agar and quantitatively under submerged fermentation. The different conditions optimized included substrate concentration, additional sugars, incubation period, temperature, initial pH, nitrogen supplementation and inoculum mass through one factor at a time approach. Maximum xylanase production was obtained at substrate concentration of (1 % xylan and 1.5 % Kolanut husk), nitrogen source (yeast extract plus peptone), carbon source (sucrose), incubation period (24 h), pH (5.0), temperature (35 oC) and inoculum size (1 %). Lysinibacillus fusiformis has been proven to be a promising bacterium for xylanase production using Kolanut husk as substrate. The use of Kolanut husk as foremost carbon source is predominantly precious as being an agricultural waste, affordable, and locally available compared to expensive commercially sold xylan.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43900272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of extracellular cellulolytic potential of selected natural strains of a novel fungus Sordaria fimicola isolated from evolutionary canyon under submerged fermentation 从进化峡谷中分离的一种新型真菌Sordaria fimicola的天然菌株在深层发酵条件下胞外纤维素分解潜力的评估

Nova Biotechnologica et Chimica Pub Date : 2022-06-24 DOI: 10.36547/nbc.1319

W. Ansari, Asifa Irshad Kayani, Amjid Khan, A. Khan, Saman Shahid, Zainab Shahzadi, Hanan Mukhtar

{"title":"Evaluation of extracellular cellulolytic potential of selected natural strains of a novel fungus Sordaria fimicola isolated from evolutionary canyon under submerged fermentation","authors":"W. Ansari, Asifa Irshad Kayani, Amjid Khan, A. Khan, Saman Shahid, Zainab Shahzadi, Hanan Mukhtar","doi":"10.36547/nbc.1319","DOIUrl":"https://doi.org/10.36547/nbc.1319","url":null,"abstract":"Industrial biotechnology has a great emerging demand and sustainable expansion for mankind to utilize a variety of biodegradable material for the production of various alternative energy resources such as biogas and bioethanol. Researchers are interested in exploitation of novel fungal strains for the production of extracellular cellulase from the last few decades. This study was designed to assess the extracellular cellulase production potential of novel fungal strains of Sordaria fimicola first isolated from the evolution canyon, three located on South facing slope with xeric (S1, S2, S3) and other three on North facing slope with mesic (N5, N6, N7) environmental conditions. Based on initial and secondary screening two hyper producer strains from each slope were selected. The best activity for S2 was 3.125 U/ml and N6 exhibited 2.829 U/mL under optimized conditions of 14 d of incubation at 30 oC, pH 6.0, 1 mL inoculum and with 2% substrate (carboxy-methyl cellulose) concentration. Among the tested carbon and nitrogen sources, glucose proved to be best for both strains with S2 exhibiting maximum activity. Peptone and beef extract proved to be the best nitrogen sources for S2 and N6 respectively. The cellulase after characterization for temperature and pH showed slightly thermophilic nature. The cellulase was partial purification the highest cellulase activities as surviving in more xeric conditions which contribute more resistive and productive features in those microorganisms living in the harsh environment.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43121434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption of Zn (II) ions from refinery wastewater by sulfuric acid-modified bentonite: Kinetic and isotherm studies 硫酸改性膨润土吸附炼化废水中锌离子的动力学和等温研究

Nova Biotechnologica et Chimica Pub Date : 2022-06-10 DOI: 10.36547/nbc.1267

D. Bellache, K. Moussaceb, J. Bollinger, F. Boudrahem

引用次数: 0