Nigerian Journal of Biotechnology最新文献_第10页

Effects Of Different Sterilization Regimes & Growth Regulators On Micropropagation Of Female Date Palm (Phoenix dactylifera L.) 不同灭菌制度及生长调节剂对枣椰树雌苗微繁的影响

Nigerian Journal of Biotechnology Pub Date : 2020-08-28 DOI: 10.4314/njb.v37i1.17

B. O. Emoghene, M. Idu, C. R. Eke, O. Asemota

{"title":"Effects Of Different Sterilization Regimes & Growth Regulators On Micropropagation Of Female Date Palm (Phoenix dactylifera L.)","authors":"B. O. Emoghene, M. Idu, C. R. Eke, O. Asemota","doi":"10.4314/njb.v37i1.17","DOIUrl":"https://doi.org/10.4314/njb.v37i1.17","url":null,"abstract":"The success of in vitro culture techniques is always hampered by microbial contamination. The present study was carried out to develop an efficient protocol for date palm explants sterilization for successful somatic embryos induction and plantlets formation of some date palm varieties. The shoot tips were treated with different sterilizing agents at different concentrations and durations of exposure. The use of ethanol (70%), sodium hypochlorite (3.5% & 70%) and mercuric chloride (0.2%) with or without addition of Tween-20 had different effects on decontamination of the date palm explants. The percentage of explants contaminated with bacteria for sterilants 1, 2 and 4 was 18.8%, 6.3% and 6.3% respectively while 25%, 37.5%, 31.25% and 6.25% were contaminated with fungi for sterilants 1, 2, 3 and 4 respectively. Under the conditions used, a combination of antioxidants (Citric and Ascorbic acids at 100mg/l), 0.2% mercuric chloride and 3.5% sodium hypochlorite solution with 3 drops/100ml of Tween-20 helped in the reduction of chlorosis, contamination and die-back in the shoot tip explants. The explants were further cultured in appropriate media for callus initiation and subsequent somatic embryo induction. Optimal embryogenic callus was obtained from the shoot explant of sterilant number 4 which had the minimal contamination and die-back of all the cultures. After 3 subcultures, the somatic embryos formed were multiplied for shoot development. From this study, we established that the use of appropriate surface sterilant at suitable concentration and duration of exposure of date palm explant to it is indispensable for maximum responses of in vitro cultures. \u0000Keywords: Date palm, Microbial contamination, Sterilizing agents, in vitro, Somatic embryos","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"55 1","pages":"159-168"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85033784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Enterobacterial Repetitive Intergenic Consensus (ERIC) as a tool for genetic characterisation of bacterial isolates in Nigeria 肠杆菌重复基因间共识(ERIC)作为尼日利亚细菌分离株遗传特征的工具

Nigerian Journal of Biotechnology Pub Date : 2020-08-28 DOI: 10.4314/njb.v37i1.13

K. Otokunefor, C. Ogugbue, B. U. Fajoyomi

{"title":"Enterobacterial Repetitive Intergenic Consensus (ERIC) as a tool for genetic characterisation of bacterial isolates in Nigeria","authors":"K. Otokunefor, C. Ogugbue, B. U. Fajoyomi","doi":"10.4314/njb.v37i1.13","DOIUrl":"https://doi.org/10.4314/njb.v37i1.13","url":null,"abstract":"Genetic characterisation as a tool for identification of bacterial isolates in Nigeria has been on the increase in recent years, and the 16s rRNA typing has been a preferred method. Due to cost limitations, there is a need to explore other genetic options. Enterobacterial Repetitive Intergenic Consensus (ERIC) polymerase chain reaction (PCR) analysis is a PCR- only based system which offers the advantage of reduced cost. This study set out to explore the use of ERIC-PCR in genetic characterisation of some selected bacterial isolates from Nigeria and compare it with genetic characterisation using 16s rRNA sequence typing. ERIC-PCR and 16s rRNA typing were carried out on 15 isolates following previously described protocols. Using 16s rRNA typing, thirteen different bacterial species were identified of which majority (85.7%) were Gram negative, with 57.1% belonging to the Enterobacteriaceae family. Using ERIC-PCR, only 13 of the 15 isolates (86.7%) could be typed, resulting in the identification of the 13 different types. ERIC-PCR was able to accurately differentiate between two members of the Proteus species, as well as identify the organisms as similar based on the banding pattern. The results show that ERIC-PCR may play a role as a bacterial identification tool but this role might be more suited to differentiating closely related members of a genus or typing within species rather than general bacterial identification. \u0000Keywords: Genetic characterisation, 16s rRNA, ERIC-PCR, Nigeria","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"32 1","pages":"122-128"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87270297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effects of different media strengths and hormone concentrations on in-vitro regeneration of Vitellaria paradoxa C.F. Gaertn 不同培养基强度和激素浓度对黄芪体外再生的影响

Nigerian Journal of Biotechnology Pub Date : 2020-08-28 DOI: 10.4314/njb.v37i1.16

J. Afolabi, E. M. Olorode, D. B. Olomola, Y. O. Fasakin, E. Adekunle

{"title":"Effects of different media strengths and hormone concentrations on in-vitro regeneration of Vitellaria paradoxa C.F. Gaertn","authors":"J. Afolabi, E. M. Olorode, D. B. Olomola, Y. O. Fasakin, E. Adekunle","doi":"10.4314/njb.v37i1.16","DOIUrl":"https://doi.org/10.4314/njb.v37i1.16","url":null,"abstract":"The importance of Vitellaria paradoxa has necessitated its inclusion in the ongoing afforestation project which requires mass production of its seedlings. The shea-butter tree produces oil-rich seeds that are important to cosmetic and food and nutrition industries. It is a deciduous tree which could survive in the dry-savannah and help withstand desert encroachment. However, overexploitation of its timber for firewood and charcoal production made it vulnerable. In-vitro propagation of this species could be a viable means towards its mass propagation. Two plant-hormones: Gibberellic Acid (GA3) and Benzyl Amino Purine (BAP) were used to grow the plant in different combinations making up six treatments (A-F) with 6 replicates in each group. The combinations include 1.5/0, 1.5/1.0, 1.5/1.5, 2.0/0, 2.0/1.0, 2.0/1.5 mg/L of GA3/BAP, which were used to grow 2cm nodal cuttings of the V. paradoxa on half-strength Murashige and Skoog (MS) media. Treatment F (with 2.0/1.5 mg/L GA3/BAP) produced the highest number of leaves (2.4 ± 0.6, 3.2 ± 0.8) and longest shoot length (1.1 ± 0.3 cm, 1.4 ± 0.5 cm) at 4 and 8 Weeks After Inoculation (WAI), respectively. The results of this study showed that application of growth- enhancing hormones for the regeneration of important tree species could provide means for their mass propagation so as to meet the need for afforestation projects. \u0000Keywords: Vitellaria paradoxa, Afforestation, In-vitro propagation, Mass propagation.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"19 1","pages":"150-158"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85218812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

FAMEs Profile of Oil Produced by Oleaginous Fungi Isolated from Fermented Beverage Wastewaters and Soil 从发酵饮料废水和土壤中分离的产油真菌产油的名目分布

Nigerian Journal of Biotechnology Pub Date : 2020-08-28 DOI: 10.4314/njb.v37i1.15

I. Y. Nsa, G. Adeloye, A. Odunsi, B. T. Akinyemi, J. T. Tubonemi, M. Saliu, J. P. Adepoju

{"title":"FAMEs Profile of Oil Produced by Oleaginous Fungi Isolated from Fermented Beverage Wastewaters and Soil","authors":"I. Y. Nsa, G. Adeloye, A. Odunsi, B. T. Akinyemi, J. T. Tubonemi, M. Saliu, J. P. Adepoju","doi":"10.4314/njb.v37i1.15","DOIUrl":"https://doi.org/10.4314/njb.v37i1.15","url":null,"abstract":"Fungal strains isolated from fermented maize (ogi) (PW) and sorghum-based brewery wastewaters (BW) and two soil isolates were evaluated for oleaginicity. The fungal isolates from the wastewater that had lipid content of at least 20% of their biomass were identified by both culture methods and internal transcribed spacer (ITS) 1-5.8S-ITS2 ribosomal DNA sequencing. The isolates were identified to be Aspergillus fumigatus (PW8), Aspergillus flavus (PW10), Candida tropicalis (PW16) and Aspergillus tubingensis (PW3), Trichosporon luoberi (BW7), Aspergillus sp. (BW4) and Candida tropicalis (BW1; BW3). FAMEs composition was determined for the four strains with the highest lipid content by acidcatalyzed transesterification and analyzed by Gas Chromatography-Flame Ionization Detector (GC-FID). Palmitoleic acid was the dominant fatty acid in M. circinelloides and T. reesei, and the best producers of capric and lauric acids were Aspergillus fumigatus and Aspergillus sp. (BW4), respectively. These fatty acids are beneficial in making cosmetics and pharmaceuticals (antimicrobials and dietary supplements). The analysis of the FAMEs profile in the species indicated low amounts or absence of some key long chain fatty acid (LCFA) constituents of biodiesels. Based on the FAMEs profile of M. circinelloides investigated, this strain could hold promise for use as feedstock for biodiesel with genetic engineering and a tailored lipid production favouring enrichment of LCFA.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"123 2","pages":"138-149"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91416547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Potentials of Indigenous Bacillus thuringiensis Isolates from the soil in controlling Fusarium wilt of Cucumber cause by Fusarium oxysporum f.sp cucumerinum 土源苏云金芽孢杆菌对黄瓜尖孢镰刀菌枯萎病的防治潜力

Nigerian Journal of Biotechnology Pub Date : 2020-08-28 DOI: 10.4314/njb.v37i1.14

P. Akintokun, A. Okuwa, A. Oloyede, S. Adebajo, A. Akintokun

{"title":"Potentials of Indigenous Bacillus thuringiensis Isolates from the soil in controlling Fusarium wilt of Cucumber cause by Fusarium oxysporum f.sp cucumerinum","authors":"P. Akintokun, A. Okuwa, A. Oloyede, S. Adebajo, A. Akintokun","doi":"10.4314/njb.v37i1.14","DOIUrl":"https://doi.org/10.4314/njb.v37i1.14","url":null,"abstract":"Cucumber (Cucumis sativus L.) production is generally low in Nigeria due to continuous soil nutrient limitation and diseases. However, the persistence in the use of agrochemicals for cucumber production in Nigeria is associated with high cost and deleterious effects on man, animal and the environment. This study was conducted to investigate the potentials of indigenous Bacillus thuringiensis (Bt), a spore-forming bacterium known for its insecticidal properties in controlling Fusarium wilt of cucumber. Bacillus thuringiensis strains were isolated from soil samples collected from different farm sites in Abeokuta, Nigeria, and identified phenotypically and molecularly. The in-vitro antagonistic activity of B. thuringiensis strains on F. oxysporum f.sp. cucumerinum was evaluated by dual culture method, followed by pot experiment in the screen house. 16S rRNA gene sequencing was performed on the antagonistic B. thuringiensis to confirm Bt species. The results of the in-vitro antagonistic activity revealed that most indigenous B. thuringiensis strains showed significant growth inhibition of Fusarium oxysporium f. sp. cucumerinum. Similarly, application of B. thuringiensis A and C isolates significantly suppressed the incidence of Fusarium wilt of cucumber in the screen house when compared to the control. The 16S rRNA gene sequencing technique identified the isolates A and C as Bacillus thuringiensis strain LTS-209 and Bacillus thuringiensis strain VITSJ-01, respectively. Hence, indigenous B. thuringiensis A and C isolates should be incorporated into cucumber cultivation for controlling Fusarium wilt disease of cucumber.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"1 1","pages":"129-137"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73420481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Epidemiological Investigation, Serotypes and Distribution of Verocytotoxigenic Escherichia coli (VTEC) in Raw Milk and Milk Products in Uyo, Nigeria 尼日利亚尤约地区原料奶和奶制品中产毒大肠杆菌(VTEC)的流行病学调查、血清型和分布

Nigerian Journal of Biotechnology Pub Date : 2020-08-28 DOI: 10.4314/njb.v37i1.2

O. J. Akinjogunla, B. Akaka, C. U. Inyang

{"title":"Epidemiological Investigation, Serotypes and Distribution of Verocytotoxigenic Escherichia coli (VTEC) in Raw Milk and Milk Products in Uyo, Nigeria","authors":"O. J. Akinjogunla, B. Akaka, C. U. Inyang","doi":"10.4314/njb.v37i1.2","DOIUrl":"https://doi.org/10.4314/njb.v37i1.2","url":null,"abstract":"Food borne diseases are of great concern globally especially in the developing countries where poor sanitation is applied during collection and processing of milk from animals. The epidemiological investigation, serotypes and distribution of verocytotoxin (VTI and VT2)producing Escherichia coli in raw milk and milk products were determined using structured questionnaire, Cefixime tellurite-sorbitol MacConkey agar, agglutination kits and VTECRPLA Toxin detection Kit. Out of 27 milkers, 7.4 % had primary education, 22.2 % washed the milk utensils with cold water and soap, 11.1 % washed their hands before milking, while 7.4 % milkers washed the udder of the animals before milking. All the yoghurts had the product names; 85.7 % had NAFDAC numbers; 80.0% had Batch Numbers, while 71.4 % had Manufacturer s’ Addresses. The unpasteurized milk samples had E. coli 0157 and non 0157 E. coli counts (CFU.ml) ranging from 4.0 x 10 to 1.7 x 10 and 6.0 x 10 to 2.0 x 10 , respectively, while E. coli 0157 and non 0157 E. coli counts of milk products were between 1.0 x 10 and 1.0 x 10 CFU.ml. E. coli 0157 had the highest percentage occurrence (38.3%), while E. coli 0145 had the lowest percentage occurrence (2.1%). More than 38.3% of the E. coli serotypes produced VT2, while ≥ 12.8% were VT1 producers. The occurrence of VTEC in the unpasteurized milk shows that the milkers should be enlightened on the necessary sanitary practices to adopt during milking and also post-pasteurization contamination of milk products should be avoided.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"33 1","pages":"10-20"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73426112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

IL4, IL13, GSTM1 and T1 variants and susceptibility to Schistosomiasis and associated bladder pathologies in Eggua, Nigeria 尼日利亚埃瓜的IL4、IL13、GSTM1和T1变异与血吸虫病和相关膀胱病变的易感性

Nigerian Journal of Biotechnology Pub Date : 2020-08-28 DOI: 10.4314/njb.v37i1.7

O. Onile, H. Awobode, A. Agunloye, C. Márquez-Dueñas, Cela R.G. Manning, C. Anumudu

{"title":"IL4, IL13, GSTM1 and T1 variants and susceptibility to Schistosomiasis and associated bladder pathologies in Eggua, Nigeria","authors":"O. Onile, H. Awobode, A. Agunloye, C. Márquez-Dueñas, Cela R.G. Manning, C. Anumudu","doi":"10.4314/njb.v37i1.7","DOIUrl":"https://doi.org/10.4314/njb.v37i1.7","url":null,"abstract":"Failure of the human host to elicit adequate immune responses to the adult Schistosoma haematobium worm and continuous strong inflammatory responses to the eggs have been the main causes of bladder pathology in chronic Schistosomiasis. Identification of susceptibility biomarkers for schistosomiasisassociated bladder pathology is necessary in order to detect genetic factors responsible for the infection and spread of the disease. The aim of this study was to identify candidate-biomarkers for susceptibility to schistosomiasis and its associated pathologies. A total of 371 adult participants, comprising 130 males and 241 females from Eggua community, Ogun State, Nigeria, were randomly recruited into a cross sectional study from August 2012 to May 2014. They were screened for S. haematobium ova and bladder pathologies by microscopy and ultrasonography, respectively. Human host susceptibility to schistosomiasis and its associated bladder pathologies were determined by PCR genotyping of Interleukin (IL4 and IL13) genes, and glutathione-S-transferase (GSTT1 and GSTM1) genes. The overall prevalence of S. haematobium in the population was 29.3% (108/369). Bladder pathologies were observed in 32.3% (117/362) of the population. Polymorphisms in IL 4-590 and IL 13-1055 were observed in 24.1% and 9.3% schistosomiasis cases, respectively. The IL 13-1055 polymorphism did not indicate susceptibility to schistosomiasis in males (OR 0.7, 95% CI 0.3-2.1) but a slight risk was found in females (OR 1.1, 95% CI 0.7-1.7). Participants with GSTM1 and GSTT1 polymorphisms expressed elevated risks of bladder pathologies (OR = 4.3, 95% CI 2.0 9.2 and OR = 4.2, 95% CI 1.5 – 12.0, respectively), with the pathology and schistosomiasis group having more GST polymorphisms than bladder pathologies.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"20 1","pages":"63-77"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80858832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Indole -3- Butyric Acid Induces Plant Regeneration From Stem Cuttings Of Three Medicinal Plants 吲哚-3-丁酸诱导三种药用植物茎插枝再生

Nigerian Journal of Biotechnology Pub Date : 2020-08-28 DOI: 10.4314/njb.v37i1.12

C. U. Okafor, E. U. Njoku, F. Ike, C. Onyekwuluje

{"title":"Indole -3- Butyric Acid Induces Plant Regeneration From Stem Cuttings Of Three Medicinal Plants","authors":"C. U. Okafor, E. U. Njoku, F. Ike, C. Onyekwuluje","doi":"10.4314/njb.v37i1.12","DOIUrl":"https://doi.org/10.4314/njb.v37i1.12","url":null,"abstract":"Field regeneration of three (3) medicinal plants - Securidaca longepedunculata (violet tree), Ocimum gratissimum (scent leaf) and Pterocarpus mildebraedii by means of rooting of stem cuttings at different lengths of 10 -15 cm and 15 -20 cm were studied. The effect of the plant hormone Indole-3-Butyric acid (IBA) at 5g/l was tested on rooting, bud sprout and leaf formation of the rooted stem cuttings of the plant species as well as the effect of the length of the stem cuttings on their growth and survival rates. The results of the study revealed that stem cuttings of O. gratissimum treated with IBA and the control treatment sprouted within 5 - 8 days. The treated O. gratissimum stem cuttings produced a slightly higher number of buds (2.58 ± 0.86) when compared to the control (2.00 ± 0.89) with no significant difference at P ≤ 0.05. Stem cuttings of the treated P. mildbraedii cuttings sprouted within 8 -10 days with 1.40 ± 0.37 number of buds, with the control showing no sprouts. Consequently, IBA application produced more leaves (13.00 ± 5.58) and roots (1.33 ± 0.01) than the control. However, O. gratissimum stem cuttings had the highest leaf (13.08 ± 4.47) and root numbers (135.00 ± 13.45) followed by the control with values- 13.00 ± 5.58 for leaf development and 61.66 ± 2.34 for root number respectively. Irrespective of the treatments, none of the stem cuttings of S. longepedunculata sprouted. This study showed that exogenous application of IBA to stem cuttings of the tested plants, except S. Longepedunculata, improved the root number, number of leaves and number of buds. \u0000Keywords: Stem cuttings, Indole-3-Butyric acid (IBA), Rooting","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"36 1","pages":"109-121"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75843687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Public Toilets in a tertiary institution in the Southern part of Nigeria as Potential Reservoirs of Drug Resistant Pathogens 尼日利亚南部一所高等教育机构的公共厕所是耐药病原体的潜在宿主

Nigerian Journal of Biotechnology Pub Date : 2020-08-28 DOI: 10.4314/njb.v37i1.8

K. Otokunefor, D. C. Chijioke, J. Kalio, G. Abu

{"title":"Public Toilets in a tertiary institution in the Southern part of Nigeria as Potential Reservoirs of Drug Resistant Pathogens","authors":"K. Otokunefor, D. C. Chijioke, J. Kalio, G. Abu","doi":"10.4314/njb.v37i1.8","DOIUrl":"https://doi.org/10.4314/njb.v37i1.8","url":null,"abstract":"Toilets have long been viewed as a significant potential contributor to human infectious diseases. Various studies worldwide have explored the bacterial communities associated with toilets but only few have focused on their possible role as reservoirs of drug resistant pathogens. To explore this role, four different surfaces from a pay-to-use toilet complex at a tertiary institution in the Southern part of Nigeria were sampled using the swab-rinse technique. Sample processing was done to determine bacterial load, identify bacterial types present in the samples and determine antibiotic susceptibility using standard techniques. Similar levels of bacterial contamination were observed at all the 14 sampling points ranging from 3.6×104 to 2.7×105 CFU. A higher level of contamination was generally noted on the door handles and floor surfaces. Of the ten different bacterial groups identified, Shigella sp. and Salmonella sp. were the predominant groups (20.6% each). The test isolates showed a wide rate of resistance to antibiotics, with the highest observed against ofloxacin (98.3%) and the least against ceftriaxone (44.4%). Forty-three different antibiogram patterns were detected among the test isolates. Most of the bacteria (63.2%) were associated with MAR index values greater than 0.8. This study shows that public toilets could play a role not just as a reservoir of potential pathogens but more specifically as a potential reservoir of drug resistant pathogenic microorganisms with high MAR indices. \u0000Keywords: Toilet, Reservoir, MAR index, Nigeria","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"78 1","pages":"85-93"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73615637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Microsatellite markers-based characterisation of elephant grass (Pennisetum purpureum) harvested from selected locations in South-West Nigeria 从尼日利亚西南部选定地点收获的象草(Pennisetum purpureum)的微卫星标记特征

Nigerian Journal of Biotechnology Pub Date : 2020-08-28 DOI: 10.4314/njb.v37i1.4

O. okukenu, A. Olajide, P. Dele, M. Wheto, B. Akinyemi, A. Jolaosho, B. O. Jokosenumi, T. Shonde

引用次数: 1