The Journal of surgical research最新文献_第6页

Isolated perfusion of a tubed superficial epigastric flap in a rodent model. 啮齿类动物腹部管状浅皮瓣的离体灌注。

The Journal of surgical research Pub Date : 2006-03-01 DOI: 10.1097/01253092-200603001-00045

A. O'Neill, L. Barbe, M. Randolph, F. Berthiaume

{"title":"Isolated perfusion of a tubed superficial epigastric flap in a rodent model.","authors":"A. O'Neill, L. Barbe, M. Randolph, F. Berthiaume","doi":"10.1097/01253092-200603001-00045","DOIUrl":"https://doi.org/10.1097/01253092-200603001-00045","url":null,"abstract":"BACKGROUND\u0000Isolated perfusion models can yield important data regarding metabolism of the skin. An effective model must remain stable during perfusion but respond appropriately to metabolic and vascular stimuli. We describe the design and characterization of a tubed superficial epigastric isolated perfusion flap.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Tubed superficial epigastric flaps were created in 20 male Sprague Dawley rats. Forty-eight hours later the femoral vessels were cannulated and the flaps were perfused using a Krebs-Heinseleit buffer containing albumin for a period of 2 h. In five of the flaps norepinephrine and acetylcholine were added sequentially to the perfusate to determine vascular reactivity. In a further four flaps insulin (20 U/liter) and iodoacetate (5 mM) were added to the perfusate to confirm that the flap was metabolically active and reactive. Venous outflow was collected at regular intervals and analyzed for electrolytes, lactate, and glucose content. Vascularity and skin perfusion were characterized using barium microangiography and methylene blue dye injection.\u0000\u0000\u0000RESULTS\u0000This flap model was found to be stable in terms of arterial pressure, electrolyte levels, and lactate production over the perfusion period. Norepinephrine caused a sharp increase in vascular resistance, which was reversed by administration of acetylcholine. Lactate production increased appropriately with the addition of insulin to the perfusate with a rapid decline following addition of the glycolysis inhibitor iodoacetate. There was no leakage of perfusate or significant swelling of the flap during the perfusion.\u0000\u0000\u0000CONCLUSIONS\u0000The tubed superficial epigastric artery flap makes an effective model for isolated perfusion studies of the skin with a wide range of experimental applications.","PeriodicalId":191568,"journal":{"name":"The Journal of surgical research","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128498450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional assessment of a new staple line reinforcement in lung resection. 新型钉线加固在肺切除术中的功能评价。

The Journal of surgical research Pub Date : 2005-10-01 DOI: 10.1378/CHEST.128.4_MEETINGABSTRACTS.310S

Douglas M. Downey, Michael Michel, J. Harre, Jerry W. Pratt

{"title":"Functional assessment of a new staple line reinforcement in lung resection.","authors":"Douglas M. Downey, Michael Michel, J. Harre, Jerry W. Pratt","doi":"10.1378/CHEST.128.4_MEETINGABSTRACTS.310S","DOIUrl":"https://doi.org/10.1378/CHEST.128.4_MEETINGABSTRACTS.310S","url":null,"abstract":"BACKGROUND\u0000A major complication of lung resection is prolonged leaking at the staple line. Staple-line reinforcement is performed routinely during these procedures using bovine pericardium (peri-strips) and expanded polytetrafluorethylene. Both materials have been shown previously to increase staple-line durability and reduce the overall incidence of prolonged air leaking after lung resection, specifically in lung volume-reduction surgery. Small intestinal submucosa (SIS) has had many applications in human tissues consequent to its absorption and healing profile, which are well documented in human and animal models. However, it had not been studied in reinforcement of pulmonary staple lines.\u0000\u0000\u0000MATERIALS AND METHODS\u0000We hypothesized that SIS reinforcement of staple lines in healthy lung tissue would increase durability, as determined by leak rates at increased airway pressures as compared to nonreinforced staple lines. Eight healthy juvenile Yorkshire-cross pigs were subjected to bilateral apical lung resections; one side was reinforced with SIS. The lungs were then inflated to sequentially increase intrabronchial pressures (5-75 cm H2O) for 60-second intervals while the chest was filled with saline under direct visualization monitoring for air leak.\u0000\u0000\u0000RESULTS\u0000Staple lines reinforced with porcine small intestinal submucosa had significantly better durability as determined by Kaplan-Meier survival calculations with respect to leak rate as a function of pressure.\u0000\u0000\u0000CONCLUSION\u0000Reinforcement of staple lines with SIS allows pulmonary staple lines to tolerate significantly higher intrabronchial pressures without demonstrating air leak at the staple line.","PeriodicalId":191568,"journal":{"name":"The Journal of surgical research","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132013026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Carbon monoxide can prevent acute lung injury observed after ischemia reperfusion of the lower extremities. 观察到一氧化碳对下肢缺血再灌注后急性肺损伤的预防作用。

The Journal of surgical research Pub Date : 2005-09-01 DOI: 10.1016/j.jamcollsurg.2005.06.240

C. Boutros, R. Zegdi, N. Lila, M. Cambillau, P. Fornés, A. Carpentier, Jean Noel Fabini

引用次数: 11

Viability and function of autologous and allogeneic fibroblasts seeded in dermal substitutes after implantation. 自体和异体成纤维细胞植入真皮替代物后的活力和功能。

The Journal of surgical research Pub Date : 2005-05-01 DOI: 10.1111/J.1067-1927.2005.130116AP.X

N. Morimoto, Y. Saso, K. Tomihata, Tsuguyoshi Taira, Yoshitake Takahashi, Masayoshi Ohta, Shigehiko Suzuki

{"title":"Viability and function of autologous and allogeneic fibroblasts seeded in dermal substitutes after implantation.","authors":"N. Morimoto, Y. Saso, K. Tomihata, Tsuguyoshi Taira, Yoshitake Takahashi, Masayoshi Ohta, Shigehiko Suzuki","doi":"10.1111/J.1067-1927.2005.130116AP.X","DOIUrl":"https://doi.org/10.1111/J.1067-1927.2005.130116AP.X","url":null,"abstract":"BACKGROUND\u0000Fibroblast-seeded collagen sponges have been used for the treatment of skin defects and skin ulcers. However, the viability of the fibroblasts after implantation is still unknown. The objective of this study was to investigate the viability and distribution of autologous and allogeneic fibroblasts after implantation and to clarify which type is more effective for wound healing.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Skin samples of Hartley guinea pigs were retrieved and autologous fibroblasts were isolated and cultured. Fibroblasts isolated from the skin of a Strain2 guinea pig were used as allogeneic fibroblasts. Three full-thickness wounds were created on the backs of guinea pigs and an acellular collagen sponge, a collagen sponge seeded with autologous fibroblasts, and a collagen sponge seeded with allogeneic fibroblasts were transplanted. Before implantation, fibroblasts were labeled with PKH26. The guinea pigs were sacrificed 1, 2, and 3 weeks after implantation. The epithelization and contraction of the wounds were assessed, and the viability and distribution of the seeded fibroblasts were observed in cross sections.\u0000\u0000\u0000RESULTS\u0000Three weeks after implantation, the PKH26-labeled autologous and allogeneic fibroblasts remained viable. In the wounds covered with the autologous fibroblast-seeded collagen sponge, the epithelization was fastest, and the percent wound contraction was smallest. In contrast, in the wounds covered with allogeneic fibroblasts, the epithelization was slowest and the percent contraction was largest.\u0000\u0000\u0000CONCLUSION\u0000The allogeneic fibroblasts seeded in the collagen sponge survived and remained viable on the grafted area, but did not accelerate wound healing.","PeriodicalId":191568,"journal":{"name":"The Journal of surgical research","volume":"125 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128675640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 64

Hypoxia augments gelatinase activity in a variety of adenocarcinomas in vitro. 在体外实验中，缺氧可增强多种腺癌中明胶酶的活性。

The Journal of surgical research Pub Date : 2005-01-22 DOI: 10.1006/J.JSS.2004.10.020

P. Ridgway, P. Ziprin, N. Alkhamesi, P. Paraskeva, D. Peck, A. Darzi

{"title":"Hypoxia augments gelatinase activity in a variety of adenocarcinomas in vitro.","authors":"P. Ridgway, P. Ziprin, N. Alkhamesi, P. Paraskeva, D. Peck, A. Darzi","doi":"10.1006/J.JSS.2004.10.020","DOIUrl":"https://doi.org/10.1006/J.JSS.2004.10.020","url":null,"abstract":"BACKGROUND\u0000Hypoxia within solid adenocarcinomas and protease up-regulation has been independently implicated as poor prognostic indicators in a variety of tumor types. The authors hypothesize that Matrix Metalloproteases (MMP) are up-regulated in direct response to a hypoxic environment.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Colonic (SW1222), breast (MDA-MB231), and pancreatic (PSN-1) tumor cell lines were exposed to hypoxia (1% oxygen/94% nitrogen/5% carbon dioxide) for periods of up to 24 h. Reaction to a hypoxic environment was determined via invasion across a Matrigel-coated 8-microm Transwell filter. Activity of MMP 2 and 9 was assessed using gelatin zymography. Expression of tissue inhibitor of metalloproteases 1 (TIMP-1) was quantified using ELISA (Biotrak). Correlation between protease expression and invasive capacity was determined using a specific gelatinase inhibitor (MMPI; Calbiochem).\u0000\u0000\u0000RESULTS\u0000All tumor lines demonstrated augmented invasion over 72 h (P < 0.01 all groups). Concomitant significant increase in MMP 2 and 9 activity was observed in the SW1222 and PSN-1 lines. MDA-MB231s showed increase in MMP 9 expression and in a unidentified 103-kDa gelatinase (P < 0.001). The hypoxia-augmented invasion was attenuated by the addition of a specific gelatinase inhibitor confirming interdependence.\u0000\u0000\u0000CONCLUSIONS\u0000Hypoxia induces an increased invasive capacity via gelatinase up-regulation without loss of cell viability. This suggests a mechanism explaining the poorer prognosis seen in patients with protease-secreting solid adenocarcinomas.","PeriodicalId":191568,"journal":{"name":"The Journal of surgical research","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127354012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Differential regulation of ERK1/2 and p38(MAPK) by components of the Rho signaling pathway during sphingosine-1-phosphate-induced smooth muscle cell migration. 鞘氨醇-1-磷酸诱导平滑肌细胞迁移过程中Rho信号通路组分对ERK1/2和p38(MAPK)的差异调控

The Journal of surgical research Pub Date : 2004-05-01 DOI: 10.1016/J.CARPATH.2004.03.135

I. Galaria, A. Fegley, S. Nicholl, E. Roztocil, M. Davies

引用次数: 11

PKC-zeta is essential for endotoxin-induced macrophage activation. PKC-zeta对内毒素诱导的巨噬细胞激活至关重要。

The Journal of surgical research Pub Date : 2003-10-01 DOI: 10.1016/J.JSS.2003.08.058

J. Cuschieri, K. Umanskiy, J. Solomkin

引用次数: 20

L-[1-13C] phenylalanine breath test reflects phenylalanine hydroxylase activity of the whole liver. L-[1-13C]苯丙氨酸呼气试验反映全肝苯丙氨酸羟化酶活性。

The Journal of surgical research Pub Date : 2003-04-01 DOI: 10.1016/S0016-5085(03)83795-2

Y. Ishii, Shigeru Suzuki, T. Kohno, Masaru Aoki, T. Kohno, A. Ito, T. Takayama, S. Asai

引用次数: 16

Expression of anti-apoptotic protein, Bcl-2, in liver regeneration after a partial hepatectomy. 抗凋亡蛋白Bcl-2在部分肝切除术后肝再生中的表达

The Journal of surgical research Pub Date : 2003-04-01 DOI: 10.1016/S0016-5085(03)83663-6

Y. Takushi, M. Shiraishi, E. Nozato, A. Toyoda, T. Nishimaki

引用次数: 7

Contractile activity of mouse small intestinal longitudinal smooth muscle. 小鼠小肠纵向平滑肌的收缩活动。

The Journal of surgical research Pub Date : 2003-04-01 DOI: 10.1016/S0016-5085(03)84035-0

T. Ueno, J. Duenes, L. Kost, M. Sarr

引用次数: 4