The Journal of surgical research最新文献_第4页

Functional assessment of a new staple line reinforcement in lung resection. 新型钉线加固在肺切除术中的功能评价。

The Journal of surgical research Pub Date : 2005-10-01 DOI: 10.1378/CHEST.128.4_MEETINGABSTRACTS.310S

Douglas M. Downey, Michael Michel, J. Harre, Jerry W. Pratt

{"title":"Functional assessment of a new staple line reinforcement in lung resection.","authors":"Douglas M. Downey, Michael Michel, J. Harre, Jerry W. Pratt","doi":"10.1378/CHEST.128.4_MEETINGABSTRACTS.310S","DOIUrl":"https://doi.org/10.1378/CHEST.128.4_MEETINGABSTRACTS.310S","url":null,"abstract":"BACKGROUND\u0000A major complication of lung resection is prolonged leaking at the staple line. Staple-line reinforcement is performed routinely during these procedures using bovine pericardium (peri-strips) and expanded polytetrafluorethylene. Both materials have been shown previously to increase staple-line durability and reduce the overall incidence of prolonged air leaking after lung resection, specifically in lung volume-reduction surgery. Small intestinal submucosa (SIS) has had many applications in human tissues consequent to its absorption and healing profile, which are well documented in human and animal models. However, it had not been studied in reinforcement of pulmonary staple lines.\u0000\u0000\u0000MATERIALS AND METHODS\u0000We hypothesized that SIS reinforcement of staple lines in healthy lung tissue would increase durability, as determined by leak rates at increased airway pressures as compared to nonreinforced staple lines. Eight healthy juvenile Yorkshire-cross pigs were subjected to bilateral apical lung resections; one side was reinforced with SIS. The lungs were then inflated to sequentially increase intrabronchial pressures (5-75 cm H2O) for 60-second intervals while the chest was filled with saline under direct visualization monitoring for air leak.\u0000\u0000\u0000RESULTS\u0000Staple lines reinforced with porcine small intestinal submucosa had significantly better durability as determined by Kaplan-Meier survival calculations with respect to leak rate as a function of pressure.\u0000\u0000\u0000CONCLUSION\u0000Reinforcement of staple lines with SIS allows pulmonary staple lines to tolerate significantly higher intrabronchial pressures without demonstrating air leak at the staple line.","PeriodicalId":191568,"journal":{"name":"The Journal of surgical research","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132013026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Carbon monoxide can prevent acute lung injury observed after ischemia reperfusion of the lower extremities. 观察到一氧化碳对下肢缺血再灌注后急性肺损伤的预防作用。

The Journal of surgical research Pub Date : 2005-09-01 DOI: 10.1016/j.jamcollsurg.2005.06.240

C. Boutros, R. Zegdi, N. Lila, M. Cambillau, P. Fornés, A. Carpentier, Jean Noel Fabini

引用次数: 11

Viability and function of autologous and allogeneic fibroblasts seeded in dermal substitutes after implantation. 自体和异体成纤维细胞植入真皮替代物后的活力和功能。

The Journal of surgical research Pub Date : 2005-05-01 DOI: 10.1111/J.1067-1927.2005.130116AP.X

N. Morimoto, Y. Saso, K. Tomihata, Tsuguyoshi Taira, Yoshitake Takahashi, Masayoshi Ohta, Shigehiko Suzuki

{"title":"Viability and function of autologous and allogeneic fibroblasts seeded in dermal substitutes after implantation.","authors":"N. Morimoto, Y. Saso, K. Tomihata, Tsuguyoshi Taira, Yoshitake Takahashi, Masayoshi Ohta, Shigehiko Suzuki","doi":"10.1111/J.1067-1927.2005.130116AP.X","DOIUrl":"https://doi.org/10.1111/J.1067-1927.2005.130116AP.X","url":null,"abstract":"BACKGROUND\u0000Fibroblast-seeded collagen sponges have been used for the treatment of skin defects and skin ulcers. However, the viability of the fibroblasts after implantation is still unknown. The objective of this study was to investigate the viability and distribution of autologous and allogeneic fibroblasts after implantation and to clarify which type is more effective for wound healing.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Skin samples of Hartley guinea pigs were retrieved and autologous fibroblasts were isolated and cultured. Fibroblasts isolated from the skin of a Strain2 guinea pig were used as allogeneic fibroblasts. Three full-thickness wounds were created on the backs of guinea pigs and an acellular collagen sponge, a collagen sponge seeded with autologous fibroblasts, and a collagen sponge seeded with allogeneic fibroblasts were transplanted. Before implantation, fibroblasts were labeled with PKH26. The guinea pigs were sacrificed 1, 2, and 3 weeks after implantation. The epithelization and contraction of the wounds were assessed, and the viability and distribution of the seeded fibroblasts were observed in cross sections.\u0000\u0000\u0000RESULTS\u0000Three weeks after implantation, the PKH26-labeled autologous and allogeneic fibroblasts remained viable. In the wounds covered with the autologous fibroblast-seeded collagen sponge, the epithelization was fastest, and the percent wound contraction was smallest. In contrast, in the wounds covered with allogeneic fibroblasts, the epithelization was slowest and the percent contraction was largest.\u0000\u0000\u0000CONCLUSION\u0000The allogeneic fibroblasts seeded in the collagen sponge survived and remained viable on the grafted area, but did not accelerate wound healing.","PeriodicalId":191568,"journal":{"name":"The Journal of surgical research","volume":"125 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128675640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 64

Hypoxia augments gelatinase activity in a variety of adenocarcinomas in vitro. 在体外实验中，缺氧可增强多种腺癌中明胶酶的活性。

The Journal of surgical research Pub Date : 2005-01-22 DOI: 10.1006/J.JSS.2004.10.020

P. Ridgway, P. Ziprin, N. Alkhamesi, P. Paraskeva, D. Peck, A. Darzi

{"title":"Hypoxia augments gelatinase activity in a variety of adenocarcinomas in vitro.","authors":"P. Ridgway, P. Ziprin, N. Alkhamesi, P. Paraskeva, D. Peck, A. Darzi","doi":"10.1006/J.JSS.2004.10.020","DOIUrl":"https://doi.org/10.1006/J.JSS.2004.10.020","url":null,"abstract":"BACKGROUND\u0000Hypoxia within solid adenocarcinomas and protease up-regulation has been independently implicated as poor prognostic indicators in a variety of tumor types. The authors hypothesize that Matrix Metalloproteases (MMP) are up-regulated in direct response to a hypoxic environment.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Colonic (SW1222), breast (MDA-MB231), and pancreatic (PSN-1) tumor cell lines were exposed to hypoxia (1% oxygen/94% nitrogen/5% carbon dioxide) for periods of up to 24 h. Reaction to a hypoxic environment was determined via invasion across a Matrigel-coated 8-microm Transwell filter. Activity of MMP 2 and 9 was assessed using gelatin zymography. Expression of tissue inhibitor of metalloproteases 1 (TIMP-1) was quantified using ELISA (Biotrak). Correlation between protease expression and invasive capacity was determined using a specific gelatinase inhibitor (MMPI; Calbiochem).\u0000\u0000\u0000RESULTS\u0000All tumor lines demonstrated augmented invasion over 72 h (P < 0.01 all groups). Concomitant significant increase in MMP 2 and 9 activity was observed in the SW1222 and PSN-1 lines. MDA-MB231s showed increase in MMP 9 expression and in a unidentified 103-kDa gelatinase (P < 0.001). The hypoxia-augmented invasion was attenuated by the addition of a specific gelatinase inhibitor confirming interdependence.\u0000\u0000\u0000CONCLUSIONS\u0000Hypoxia induces an increased invasive capacity via gelatinase up-regulation without loss of cell viability. This suggests a mechanism explaining the poorer prognosis seen in patients with protease-secreting solid adenocarcinomas.","PeriodicalId":191568,"journal":{"name":"The Journal of surgical research","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127354012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Differential regulation of ERK1/2 and p38(MAPK) by components of the Rho signaling pathway during sphingosine-1-phosphate-induced smooth muscle cell migration. 鞘氨醇-1-磷酸诱导平滑肌细胞迁移过程中Rho信号通路组分对ERK1/2和p38(MAPK)的差异调控

The Journal of surgical research Pub Date : 2004-05-01 DOI: 10.1016/J.CARPATH.2004.03.135

I. Galaria, A. Fegley, S. Nicholl, E. Roztocil, M. Davies

引用次数: 11

PKC-zeta is essential for endotoxin-induced macrophage activation. PKC-zeta对内毒素诱导的巨噬细胞激活至关重要。

The Journal of surgical research Pub Date : 2003-10-01 DOI: 10.1016/J.JSS.2003.08.058

J. Cuschieri, K. Umanskiy, J. Solomkin

引用次数: 20

L-[1-13C] phenylalanine breath test reflects phenylalanine hydroxylase activity of the whole liver. L-[1-13C]苯丙氨酸呼气试验反映全肝苯丙氨酸羟化酶活性。

The Journal of surgical research Pub Date : 2003-04-01 DOI: 10.1016/S0016-5085(03)83795-2

Y. Ishii, Shigeru Suzuki, T. Kohno, Masaru Aoki, T. Kohno, A. Ito, T. Takayama, S. Asai

引用次数: 16

Expression of anti-apoptotic protein, Bcl-2, in liver regeneration after a partial hepatectomy. 抗凋亡蛋白Bcl-2在部分肝切除术后肝再生中的表达

The Journal of surgical research Pub Date : 2003-04-01 DOI: 10.1016/S0016-5085(03)83663-6

Y. Takushi, M. Shiraishi, E. Nozato, A. Toyoda, T. Nishimaki

引用次数: 7

Contractile activity of mouse small intestinal longitudinal smooth muscle. 小鼠小肠纵向平滑肌的收缩活动。

The Journal of surgical research Pub Date : 2003-04-01 DOI: 10.1016/S0016-5085(03)84035-0

T. Ueno, J. Duenes, L. Kost, M. Sarr

引用次数: 4

Oral folic acid improves endothelial dysfunction in cigarette smokers. 口服叶酸可改善吸烟者内皮功能障碍。

The Journal of surgical research Pub Date : 2002-08-01 DOI: 10.1006/JSRE.2002.6467

O'Grady Hl, Austin Leahy, Paul McCormick, Patricia Fitzgerald, Kelly Ck, D. Bouchier‐Hayes

{"title":"Oral folic acid improves endothelial dysfunction in cigarette smokers.","authors":"O'Grady Hl, Austin Leahy, Paul McCormick, Patricia Fitzgerald, Kelly Ck, D. Bouchier‐Hayes","doi":"10.1006/JSRE.2002.6467","DOIUrl":"https://doi.org/10.1006/JSRE.2002.6467","url":null,"abstract":"INTRODUCTION\u0000Endothelial dysfunction is an early manifestation of the atheromatous process and is evident without overt clinical signs or symptoms of the disease. Cigarette smoking has been shown to be associated with endothelial dysfunction in otherwise healthy adults. Although cessation of smoking is the ideal objective, it is not always attainable, and therefore any strategy to prevent early endothelial dysfunction is desirable. Folic acid is currently under review as a rational therapeutic agent in hyperhomocysteinemia. However, folic acid may modify endothelial function independent of its effect on homocysteine. We therefore investigated the effect of folic acid on endothelial function in young otherwise healthy cigarette smokers.\u0000\u0000\u0000METHODS\u0000Volunteer cigarette smokers (n = 10) and control lifelong nonsmokers were enrolled in the study. Baseline folate, vitamin B12, homocysteine, and cholesterol levels were analyzed. Flow-mediated dilatation, an endothelial-dependent phenomenon, was assessed using ultrasonography. This scan was performed at baseline and following 4 weeks of folic acid supplementation (5 mg/day).\u0000\u0000\u0000RESULTS\u0000There were no significant differences in the baseline hematological investigations between the groups. Homocysteine levels were within normal limits in both groups and did not change following folic acid supplementation. Cigarette smokers demonstrated significant endothelial dysfunction compared to controls (P < 0.005). This difference was significantly attenuated by folic acid supplementation (P < 0.005).\u0000\u0000\u0000CONCLUSION\u0000Folic acid significantly improves endothelial function in otherwise healthy cigarette smokers. This provides a potential therapeutic tool in attenuating the atheromatous process in this group.","PeriodicalId":191568,"journal":{"name":"The Journal of surgical research","volume":"150 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120772522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22