Neuron glia biology最新文献_第5页

The specific role of histone deacetylase 2 in adult neurogenesis. 组蛋白去乙酰化酶2在成人神经发生中的特殊作用。

Neuron glia biology Pub Date : 2010-05-01 Epub Date: 2010-04-14 DOI: 10.1017/S1740925X10000049

Melanie Jawerka, Dilek Colak, Leda Dimou, Carmen Spiller, Sabine Lagger, Rusty L Montgomery, Eric N Olson, Wolfgang Wurst, Martin Göttlicher, Magdalena Götz

{"title":"The specific role of histone deacetylase 2 in adult neurogenesis.","authors":"Melanie Jawerka, Dilek Colak, Leda Dimou, Carmen Spiller, Sabine Lagger, Rusty L Montgomery, Eric N Olson, Wolfgang Wurst, Martin Göttlicher, Magdalena Götz","doi":"10.1017/S1740925X10000049","DOIUrl":"https://doi.org/10.1017/S1740925X10000049","url":null,"abstract":"Gene expression changes during cell differentiation are thought to be coordinated by histone modifications, but still little is known about the role of specific histone deacetylases (HDACs) in cell fate decisions in vivo. Here we demonstrate that the catalytic function of HDAC2 is required in adult, but not embryonic neurogenesis. While brain development and adult stem cell fate were normal upon conditional deletion of HDAC2 or in mice lacking the catalytic activity of HDAC2, neurons derived from both zones of adult neurogenesis die at a specific maturation stage. This phenotype is correlated with an increase in proliferation and the aberrant maintenance of proteins normally expressed only in progenitors, such as Sox2, also into some differentiating neurons, suggesting that HDAC2 is critically required to silence progenitor transcripts during neuronal differentiation of adult generated neurons. This cell-autonomous function of HDAC2 exclusively in adult neurogenesis reveals clear differences in the molecular mechanisms regulating neurogenesis during development and in adulthood.","PeriodicalId":19153,"journal":{"name":"Neuron glia biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S1740925X10000049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28918694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 110

The structure of the perineuronal sheath of satellite glial cells (SGCs) in sensory ganglia. 感觉神经节卫星胶质细胞(SGCs)神经元周围鞘的结构。

Neuron glia biology Pub Date : 2010-02-01 DOI: 10.1017/S1740925X10000037

Ennio Pannese

{"title":"The structure of the perineuronal sheath of satellite glial cells (SGCs) in sensory ganglia.","authors":"Ennio Pannese","doi":"10.1017/S1740925X10000037","DOIUrl":"https://doi.org/10.1017/S1740925X10000037","url":null,"abstract":"In sensory ganglia each nerve cell body is usually enveloped by a satellite glial cell (SGC) sheath, sharply separated from sheaths encircling adjacent neurons by connective tissue. However, following axon injury SGCs may form bridges connecting previously separate perineuronal sheaths. Each sheath consists of one or several layers of cells that overlap in a more or less complex fashion; sometimes SGCs form a perineuronal myelin sheath. SGCs are flattened mononucleate cells containing the usual cell organelles. Several ion channels, receptors and adhesion molecules have been identified in these cells. SGCs of the same sheath are usually linked by adherent and gap junctions, and are functionally coupled. Following axon injury, both the number of gap junctions and the coupling of SGCs increase markedly. The apposed plasma membranes of adjacent cells are separated by 15-20 nm gaps, which form a potential pathway, usually long and tortuous, between connective tissue and neuronal surface. The boundary between neuron and SGC sheath is usually complicated, mainly by many projections arising from the neuron. The outer surface of the SGC sheath is covered by a basal lamina. The number of SGCs enveloping a nerve cell body is proportional to the cell body volume; the volume of the SGC sheath is proportional to the volume and surface area of the nerve cell body. In old animals, both the number of SGCs and the mean volume of the SGC sheaths are significantly lower than in young adults. Furthermore, extensive portions of the neuronal surface are not covered by SGCs, exposing neurons of aged animals to damage by harmful substances.","PeriodicalId":19153,"journal":{"name":"Neuron glia biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S1740925X10000037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29104032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 119

Expression and contribution of satellite glial cells purinoceptors to pain transmission in sensory ganglia: an update. 卫星神经胶质细胞嘌呤受体在感觉神经节疼痛传递中的表达和贡献:最新进展。

Neuron glia biology Pub Date : 2010-02-01 DOI: 10.1017/S1740925X10000086

Giovanni Villa, Marta Fumagalli, Claudia Verderio, Maria P Abbracchio, Stefania Ceruti

{"title":"Expression and contribution of satellite glial cells purinoceptors to pain transmission in sensory ganglia: an update.","authors":"Giovanni Villa, Marta Fumagalli, Claudia Verderio, Maria P Abbracchio, Stefania Ceruti","doi":"10.1017/S1740925X10000086","DOIUrl":"https://doi.org/10.1017/S1740925X10000086","url":null,"abstract":"The role of adenosine-5'-triphosphate (ATP) and of the ligand-gated P2X3 receptor in neuronal dorsal root ganglia (DRG) pain transmission is relatively well established. Much less is known about the purinergic system in trigeminal ganglia (TG), which are involved in certain types of untreatable neuropathic and inflammatory pain, as well as in migraine. Emerging data suggest that purinergic metabotropic P2Y receptors on both neurons and satellite glial cells (SGCs) may also participate in both physiological and pathological pain development. Here, we provide an updated literature review on the role of purinergic signaling in sensory ganglia, with special emphasis on P2Y receptors on SGCs. We also provide new original data showing a time-dependent downregulation of P2Y2 and P2Y4 receptor expression and function in purified SGCs cultures from TG, in comparison with primary mixed neuron-SGCs cultures. These data highlight the importance of the neuron-glia cross-talk in determining the SGCs phenotype. Finally, we show that, in mixed TG cultures, both adenine and guanosine induce intracellular calcium transients in neurons but not in SGCs, suggesting that also these purinergic-related molecules can participate in pain signaling. These findings may have relevant implications for the development of new therapeutic strategies for chronic pain treatment.","PeriodicalId":19153,"journal":{"name":"Neuron glia biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S1740925X10000086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29104033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 61

The role of glial cells in influencing neurite extension by dorsal root ganglion cells. 神经胶质细胞在影响背根神经节细胞的神经突延伸中的作用。

Neuron glia biology Pub Date : 2010-02-01 Epub Date: 2009-12-22 DOI: 10.1017/S1740925X09990433

Kai-Yu Ng, Yung H Wong, Helen Wise

{"title":"The role of glial cells in influencing neurite extension by dorsal root ganglion cells.","authors":"Kai-Yu Ng, Yung H Wong, Helen Wise","doi":"10.1017/S1740925X09990433","DOIUrl":"https://doi.org/10.1017/S1740925X09990433","url":null,"abstract":"When pretreated with pertussis toxin (PTX), the neurites of adult rat dorsal root ganglion (DRG) cells in mixed cell cultures retract over a period of 2 h following the initial stimulus of removal from the cell culture incubator for brief periods of observation. The purpose of this investigation was to determine whether this PTX-dependent response was specific to any one of the three subpopulations of DRG neurons. However, no neurite retraction response was observed in neuron-enriched populations of cells, or in cultures enriched in isolectin B4 (IB4)-positive neurons or in IB4-negative neurons. But, the addition of non-neuronal cells, and/or medium conditioned by non-neuronal cells, was sufficient to restore the PTX-dependent neurite retraction response, but only in large diameter IB4-negative neurons. In conclusion, we have identified a regulatory response, mediated by Gi/o-proteins, which prevents retraction of neurites in large diameter IB4-negative cells of adult rat DRG. The non-neuronal cells of adult rat DRG constitutively release factor/s that can stimulate neurite retraction of a subset of isolated DRG neurons, but this property of non-neuronal cells is only observed when the Gi/o-proteins of large diameter IB4-negative cells are inhibited.","PeriodicalId":19153,"journal":{"name":"Neuron glia biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S1740925X09990433","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28606894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Peripheral inflammation augments gap junction-mediated coupling among satellite glial cells in mouse sympathetic ganglia. 外周炎症增强小鼠交感神经节卫星胶质细胞间隙连接介导的偶联。

Neuron glia biology Pub Date : 2010-02-01 Epub Date: 2010-03-05 DOI: 10.1017/S1740925X10000025

Menachem Hanani, Anna Caspi, Vitali Belzer

引用次数: 20

Neuronal soma-satellite glial cell interactions in sensory ganglia and the participation of purinergic receptors. 感觉神经节中神经元体-卫星胶质细胞的相互作用及嘌呤能受体的参与。

Neuron glia biology Pub Date : 2010-02-01 DOI: 10.1017/S1740925X10000116

Yanping Gu, Yong Chen, Xiaofei Zhang, Guang-Wen Li, Congying Wang, Li-Yen Mae Huang

{"title":"Neuronal soma-satellite glial cell interactions in sensory ganglia and the participation of purinergic receptors.","authors":"Yanping Gu, Yong Chen, Xiaofei Zhang, Guang-Wen Li, Congying Wang, Li-Yen Mae Huang","doi":"10.1017/S1740925X10000116","DOIUrl":"https://doi.org/10.1017/S1740925X10000116","url":null,"abstract":"It has been known for some time that the somata of neurons in sensory ganglia respond to electrical or chemical stimulation and release transmitters in a Ca2+-dependent manner. The function of the somatic release has not been well delineated. A unique characteristic of the ganglia is that each neuronal soma is tightly enwrapped by satellite glial cells (SGCs). The somatic membrane of a sensory neuron rarely makes synaptic contact with another neuron. As a result, the influence of somatic release on the activity of adjacent neurons is likely to be indirect and/or slow. Recent studies of neuron-SGC interactions have demonstrated that ATP released from the somata of dorsal root ganglion neurons activates SGCs. They in turn exert complex excitatory and inhibitory modulation of neuronal activity. Thus, SGCs are actively involved in the processing of afferent information. In this review, we summarize our understanding of bidirectional communication between neuronal somata and SGCs in sensory ganglia and its possible role in afferent signaling under normal and injurious conditions. The participation of purinergic receptors is emphasized because of their dominant roles in the communication.","PeriodicalId":19153,"journal":{"name":"Neuron glia biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S1740925X10000116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29102837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 87

Bidirectional calcium signaling between satellite glial cells and neurons in cultured mouse trigeminal ganglia. 三叉神经节卫星胶质细胞与神经元间的双向钙信号传导。

Neuron glia biology Pub Date : 2010-02-01 Epub Date: 2009-11-06 DOI: 10.1017/S1740925X09990408

Sylvia O Suadicani, Pavel S Cherkas, Jonathan Zuckerman, David N Smith, David C Spray, Menachem Hanani

{"title":"Bidirectional calcium signaling between satellite glial cells and neurons in cultured mouse trigeminal ganglia.","authors":"Sylvia O Suadicani, Pavel S Cherkas, Jonathan Zuckerman, David N Smith, David C Spray, Menachem Hanani","doi":"10.1017/S1740925X09990408","DOIUrl":"https://doi.org/10.1017/S1740925X09990408","url":null,"abstract":"Astrocytes communicate with neurons, endothelial and other glial cells through transmission of intercellular calcium signals. Satellite glial cells (SGCs) in sensory ganglia share several properties with astrocytes, but whether this type of communication occurs between SGCs and sensory neurons has not been explored. In the present work we used cultured neurons and SGCs from mouse trigeminal ganglia to address this question. Focal electrical or mechanical stimulation of single neurons in trigeminal ganglion cultures increased intracellular calcium concentration in these cells and triggered calcium elevations in adjacent glial cells. Similar to neurons, SGCs responded to mechanical stimulation with increase in cytosolic calcium that spread to the adjacent neuron and neighboring glial cells. Calcium signaling from SGCs to neurons and among SGCs was diminished in the presence of the broad-spectrum P2 receptor antagonist suramin (50 muM) or in the presence of the gap junction blocker carbenoxolone (100 muM), whereas signaling from neurons to SGCs was reduced by suramin, but not by carbenoxolone. Following induction of submandibular inflammation by Complete Freund's Adjuvant injection, the amplitude of signaling among SGCs and from SGCs to neuron was increased, whereas the amplitude from neuron to SGCs was reduced. These results indicate for the first time the presence of bidirectional calcium signaling between neurons and SGCs in sensory ganglia cultures, which is mediated by the activation of purinergic P2 receptors, and to some extent by gap junctions. Furthermore, the results indicate that not only sensory neurons, but also SGCs release ATP. This form of intercellular calcium signaling likely plays key roles in the modulation of neuronal activity within sensory ganglia in normal and pathological states.","PeriodicalId":19153,"journal":{"name":"Neuron glia biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S1740925X09990408","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28490392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 126

Satellite glial cells express IL-6 and corresponding signal-transducing receptors in the dorsal root ganglia of rat neuropathic pain model. 大鼠神经性疼痛模型背根神经节卫星胶质细胞表达IL-6及相应的信号转导受体。

Neuron glia biology Pub Date : 2010-02-01 Epub Date: 2010-06-02 DOI: 10.1017/S1740925X10000074

Petr Dubový, Ilona Klusáková, Ivana Svízenská, Václav Brázda

{"title":"Satellite glial cells express IL-6 and corresponding signal-transducing receptors in the dorsal root ganglia of rat neuropathic pain model.","authors":"Petr Dubový, Ilona Klusáková, Ivana Svízenská, Václav Brázda","doi":"10.1017/S1740925X10000074","DOIUrl":"https://doi.org/10.1017/S1740925X10000074","url":null,"abstract":"There is a growing body of evidence that cytokines contribute to both induction and maintenance of neuropathic pain derived from changes in dorsal root ganglia (DRG), including the activity of the primary sensory neurons and their satellite glial cells (SGC). We used immunofluorescence and in situ hybridization methods to provide evidence that chronic constriction injury (CCI) of the sciatic nerve induces synthesis of interleukin-6 (IL-6) in SGC, elevation of IL-6 receptor (IL-6R) and activation of signal transducer and activator of transcription 3 (STAT3) signalling. Unilateral CCI of the rat sciatic nerve induced mechanoallodynia and thermal hyperalgesia in ipsilateral hind paws, but contralateral paws exhibited only temporal changes of sensitivity. We demonstrated that IL-6 mRNA and protein, which were expressed at very low levels in naïve DRG, were bilaterally increased not only in L4-L5 DRG neurons but also in SGC activated by unilateral CCI. Besides IL-6, substantial increase of IL-6R and pSTAT3 expression occurred in SGC following CCI, however, IL-6R associated protein, gp130 levels did not change. The results may suggest that unilateral CCI of the sciatic nerve induces bilateral activation of SGC in L4-L5 DRG to transduce IL-6 signalling during neuroinflammation.","PeriodicalId":19153,"journal":{"name":"Neuron glia biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S1740925X10000074","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29030728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 97

Can satellite glial cells be therapeutic targets for pain control? 卫星神经胶质细胞能成为控制疼痛的治疗靶点吗?

Neuron glia biology Pub Date : 2010-02-01 Epub Date: 2010-06-22 DOI: 10.1017/S1740925X10000098

Luc Jasmin, Jean-Philippe Vit, Aditi Bhargava, Peter T Ohara

引用次数: 144

How do the satellite glia cells of the dorsal root ganglia respond to stressed neurons?--nitric oxide saga from embryonic development to axonal injury in adulthood. 背根神经节的卫星胶质细胞对应激神经元有何反应?——一氧化氮从胚胎发育到成年期轴突损伤的历程。

Neuron glia biology Pub Date : 2010-02-01 DOI: 10.1017/S1740925X09990494

Matthew J G Bradman, Daleep K Arora, Richard Morris, Thimmasettappa Thippeswamy

{"title":"How do the satellite glia cells of the dorsal root ganglia respond to stressed neurons?--nitric oxide saga from embryonic development to axonal injury in adulthood.","authors":"Matthew J G Bradman, Daleep K Arora, Richard Morris, Thimmasettappa Thippeswamy","doi":"10.1017/S1740925X09990494","DOIUrl":"https://doi.org/10.1017/S1740925X09990494","url":null,"abstract":"Dorsal root ganglia (DRG) respond to peripheral nerve injury by up-regulating nitric oxide (NO) production by neurons and glia in addition to local fibroblasts, endothelium and macrophages. We hypothesise that NO produced from these cells has specific roles. We have shown that when neuronal NO synthase (nNOS) is blocked in axotomised DRG, neurons undergo degenerative changes (Thippeswamy et al., 2001, 2007a). Further, we demonstrated that increased neuronal NO production, in response to axotomy/growth factor-deprivation in vitro, signals glial cells to produce trophic factors to support neuronal survival (Thippeswamy et al., 2005a). Recently, we found that treating satellite glia-neuron co-cultures with nNOS inhibitor, 7-nitroindazole (7NI), decreases the number of nestin+ cells that show neuron-like morphology. Cultured/axotomised DRG also upregulate inducible NOS (iNOS) in non-neuronal cells. Therefore, it is plausible that degenerative changes following nNOS inhibition are also due to iNOS-mediated excessive NO production by non-neuronal cells, which indeed is cytotoxic. NG-nitro-l-arginine methylester (L-NAME), the pan NOS inhibitor did not significantly change nNOS+ neuron number in axotomised DRG compared to 7NI suggesting that iNOS-mediated NO contributes to the degenerative process. In this paper, these findings from our and others' past work on NO-mediated neuron-glia signalling in axotomised DRG are discussed.","PeriodicalId":19153,"journal":{"name":"Neuron glia biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1017/S1740925X09990494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28679899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10