Nature biotechnology最新文献_第9页

Genentech, Sangamo ink Alzheimer’s deal 基因泰克与 Sangamo 签订阿尔茨海默氏症治疗协议

IF 33.1 1区生物学

Nature biotechnology Pub Date : 2024-09-13 DOI: 10.1038/s41587-024-02413-x

引用次数: 0

Dedifferentiating myoblasts back into a satellite cell state in vitro 在体外将肌母细胞重新分化为卫星细胞状态

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-09-11 DOI: 10.1038/s41587-024-02345-6

引用次数: 0

Efficient genetic code expansion without host genome modifications 无需修改宿主基因组即可高效扩展遗传密码

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-09-11 DOI: 10.1038/s41587-024-02385-y

Alan Costello, Alexander A. Peterson, David L. Lanster, Zhiyi Li, Gavriela D. Carver, Ahmed H. Badran

{"title":"Efficient genetic code expansion without host genome modifications","authors":"Alan Costello, Alexander A. Peterson, David L. Lanster, Zhiyi Li, Gavriela D. Carver, Ahmed H. Badran","doi":"10.1038/s41587-024-02385-y","DOIUrl":"https://doi.org/10.1038/s41587-024-02385-y","url":null,"abstract":"Supplementing translation with noncanonical amino acids (ncAAs) can yield protein sequences with new-to-nature functions but existing ncAA incorporation strategies suffer from low efficiency and context dependence. We uncover codon usage as a previously unrecognized contributor to efficient genetic code expansion using non-native codons. Relying only on conventional Escherichia coli strains with native ribosomes, we develop a plasmid-based codon compression strategy that minimizes context dependence and improves ncAA incorporation at quadruplet codons. We confirm that this strategy is compatible with all known genetic code expansion resources, which allowed us to identify 12 mutually orthogonal transfer RNA (tRNA)–synthetase pairs. Enabled by these findings, we evolved and optimized five tRNA–synthetase pairs to incorporate a broad repertoire of ncAAs at orthogonal quadruplet codons. Lastly, we extend these resources to an in vivo biosynthesis platform that can readily create >100 new-to-nature peptide macrocycles bearing up to three unique ncAAs. Our approach will accelerate innovations in multiplexed genetic code expansion and the discovery of chemically diverse biomolecules.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":null,"pages":null},"PeriodicalIF":46.9,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142166057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organoid culture promotes dedifferentiation of mouse myoblasts into stem cells capable of complete muscle regeneration 类器官培养促进小鼠肌母细胞向能够完全再生肌肉的干细胞进行再分化

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-09-11 DOI: 10.1038/s41587-024-02344-7

Feodor D. Price, Mark N. Matyas, Andrew R. Gehrke, William Chen, Erica A. Wolin, Kristina M. Holton, Rebecca M. Gibbs, Alice Lee, Pooja S. Singu, Jeffrey S. Sakakeeny, James M. Poteracki, Kelsey Goune, Isabella T. Pfeiffer, Sarah A. Boswell, Peter K. Sorger, Mansi Srivastava, Kathleen Lindahl Pfaff, Emanuela Gussoni, Sean M. Buchanan, Lee L. Rubin

{"title":"Organoid culture promotes dedifferentiation of mouse myoblasts into stem cells capable of complete muscle regeneration","authors":"Feodor D. Price, Mark N. Matyas, Andrew R. Gehrke, William Chen, Erica A. Wolin, Kristina M. Holton, Rebecca M. Gibbs, Alice Lee, Pooja S. Singu, Jeffrey S. Sakakeeny, James M. Poteracki, Kelsey Goune, Isabella T. Pfeiffer, Sarah A. Boswell, Peter K. Sorger, Mansi Srivastava, Kathleen Lindahl Pfaff, Emanuela Gussoni, Sean M. Buchanan, Lee L. Rubin","doi":"10.1038/s41587-024-02344-7","DOIUrl":"https://doi.org/10.1038/s41587-024-02344-7","url":null,"abstract":"Experimental cell therapies for skeletal muscle conditions have shown little success, primarily because they use committed myogenic progenitors rather than true muscle stem cells, known as satellite cells. Here we present a method to generate in vitro-derived satellite cells (idSCs) from skeletal muscle tissue. When transplanted in small numbers into mouse muscle, mouse idSCs fuse into myofibers, repopulate the satellite cell niche, self-renew, support multiple rounds of muscle regeneration and improve force production on par with freshly isolated satellite cells in damaged skeletal muscle. We compared the epigenomic and transcriptional signatures between idSCs, myoblasts and satellite cells and used these signatures to identify core signaling pathways and genes that confer idSC functionality. Finally, from human muscle biopsies, we successfully generated satellite cell-like cells in vitro. After further development, idSCs may provide a scalable source of cells for the treatment of genetic muscle disorders, trauma-induced muscle damage and age-related muscle weakness.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":null,"pages":null},"PeriodicalIF":46.9,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142166056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recoded gene circuits for multiplexed genetic code expansion 用于多路遗传密码扩展的重编码基因电路

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-09-11 DOI: 10.1038/s41587-024-02387-w

引用次数: 0

Phage genome engineering with retrons 利用 retrons 进行噬菌体基因组工程

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-09-09 DOI: 10.1038/s41587-024-02392-z

Ilya Osterman, Rotem Sorek

引用次数: 0

Continuous multiplexed phage genome editing using recombitrons 利用重组子进行连续多重噬菌体基因组编辑

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-09-05 DOI: 10.1038/s41587-024-02370-5

Chloe B. Fishman, Kate D. Crawford, Santi Bhattarai-Kline, Darshini Poola, Karen Zhang, Alejandro González-Delgado, Matías Rojas-Montero, Seth L. Shipman

{"title":"Continuous multiplexed phage genome editing using recombitrons","authors":"Chloe B. Fishman, Kate D. Crawford, Santi Bhattarai-Kline, Darshini Poola, Karen Zhang, Alejandro González-Delgado, Matías Rojas-Montero, Seth L. Shipman","doi":"10.1038/s41587-024-02370-5","DOIUrl":"https://doi.org/10.1038/s41587-024-02370-5","url":null,"abstract":"Bacteriophage genome editing can enhance the efficacy of phages to eliminate pathogenic bacteria in patients and in the environment. However, current methods for editing phage genomes require laborious screening, counterselection or in vitro construction of modified genomes. Here, we present a scalable approach that uses modified bacterial retrons called recombitrons to generate recombineering donor DNA paired with single-stranded binding and annealing proteins for integration into phage genomes. This system can efficiently create genome modifications in multiple phages without the need for counterselection. The approach also supports larger insertions and deletions, which can be combined with simultaneous counterselection for >99% efficiency. Moreover, we show that the process is continuous, with more edits accumulating the longer the phage is cultured with the host, and multiplexable. We install up to five distinct mutations on a single lambda phage genome without counterselection in only a few hours of hands-on time and identify a residue-level epistatic interaction in the T7 gp17 tail fiber.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":null,"pages":null},"PeriodicalIF":46.9,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142138048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pandemic preparedness demands proper prioritization 大流行病的防备需要适当安排优先次序

IF 33.1 1区生物学

Nature biotechnology Pub Date : 2024-09-05 DOI: 10.1038/s41587-024-02406-w

引用次数: 0

Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells 从人类诱导多能干细胞分化出的多系造血细胞的长期移植

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-09-02 DOI: 10.1038/s41587-024-02360-7

Elizabeth S. Ng, Gulcan Sarila, Jacky Y. Li, Hasindu S. Edirisinghe, Ritika Saxena, Shicheng Sun, Freya F. Bruveris, Tanya Labonne, Nerida Sleebs, Alexander Maytum, Raymond Y. Yow, Chantelle Inguanti, Ali Motazedian, Vincenzo Calvanese, Sandra Capellera-Garcia, Feiyang Ma, Hieu T. Nim, Mirana Ramialison, Constanze Bonifer, Hanna K. A. Mikkola, Edouard G. Stanley, Andrew G. Elefanty

{"title":"Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells","authors":"Elizabeth S. Ng, Gulcan Sarila, Jacky Y. Li, Hasindu S. Edirisinghe, Ritika Saxena, Shicheng Sun, Freya F. Bruveris, Tanya Labonne, Nerida Sleebs, Alexander Maytum, Raymond Y. Yow, Chantelle Inguanti, Ali Motazedian, Vincenzo Calvanese, Sandra Capellera-Garcia, Feiyang Ma, Hieu T. Nim, Mirana Ramialison, Constanze Bonifer, Hanna K. A. Mikkola, Edouard G. Stanley, Andrew G. Elefanty","doi":"10.1038/s41587-024-02360-7","DOIUrl":"https://doi.org/10.1038/s41587-024-02360-7","url":null,"abstract":"Hematopoietic stem cells (HSCs) derived from human induced pluripotent stem cells (iPS cells) have important biomedical applications. We identified differentiation conditions that generate HSCs defined by robust long-term multilineage engraftment in immune-deficient NOD,B6.Prkdcscid Il2rgtm1Wjl/SzJ KitW41/W41 mice. We guided differentiating iPS cells, as embryoid bodies in a defined culture medium supplemented with retinyl acetate, through HOXA-patterned mesoderm to hemogenic endothelium specified by bone morphogenetic protein 4 and vascular endothelial growth factor (VEGF). Removal of VEGF facilitated an efficient endothelial-to-hematopoietic transition, evidenced by release into the culture medium of CD34+ blood cells, which were cryopreserved. Intravenous transplantation of two million thawed CD34+ cells differentiated from four independent iPS cell lines produced multilineage bone marrow engraftment in 25–50% of immune-deficient recipient mice. These functionally defined, multipotent CD34+ hematopoietic cells, designated iPS cell-derived HSCs (iHSCs), produced levels of engraftment similar to those achieved following umbilical cord blood transplantation. Our study provides a step toward the goal of generating HSCs for clinical translation.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":null,"pages":null},"PeriodicalIF":46.9,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142118190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editor’s pick: Atomic AI 编辑推荐原子人工智能

IF 33.1 1区生物学

Nature biotechnology Pub Date : 2024-08-26 DOI: 10.1038/s41587-024-02375-0

Vivien Marx

引用次数: 0