Archives of Medical Science : AMS最新文献_第8页

Is percutaneous computed tomography-guided biopsy sufficient to establish indolent lymphoma transformation? 经皮计算机断层扫描引导下的活检是否足以确定惰性淋巴瘤转化?

Archives of Medical Science : AMS Pub Date : 2018-12-31 DOI: 10.5114/aoms.2018.79573

P. Dvořák, P. Hoffmann, M. Šimkovič, Jiří Jandura, M. Nová

{"title":"Is percutaneous computed tomography-guided biopsy sufficient to establish indolent lymphoma transformation?","authors":"P. Dvořák, P. Hoffmann, M. Šimkovič, Jiří Jandura, M. Nová","doi":"10.5114/aoms.2018.79573","DOIUrl":"https://doi.org/10.5114/aoms.2018.79573","url":null,"abstract":"Introduction The aim of the study was to retrospectively evaluate the technical features, efficacy, accuracy, and relevant complications of computed tomography-guided biopsies in various anatomical localizations when diagnosing indolent lymphoma transformations, relapses, duplicate malignant diseases or benign processes. Material and methods From December 2007 to December 2017, 81 percutaneous biopsy procedures in 72 patients for tumors, sizes 17–232 mm in diameter (median length: 39 mm), were performed in patients with known indolent lymphomas in their clinical history. The patients were men in 41 cases and women in 31 cases, aged 36 to 86 years. Results In 79 cases (97.5%; 95% CI: 91.3–99.7) results were true positive or true negative; only 2 interventions (2.5%; 95% CI: 0.3–8.6) were histologically false negative. Transformation was verified in 29 cases (35.8%; 95% CI: 25.4–47.2), relapses in 30 cases (37%; 95% CI: 26.6–48.5), duplicate malignancy in 15 cases (18.5%; 95% CI: 10.8–28.7) and benign processes in 7 cases (8.7%; 95% CI: 3.5–17.0). Eight complications in total were revealed, 7 of which were in consequence of thoracic cavity biopsy. A statistically significant relationship between the complication incidence and anatomical localization in the thoracic cavity was identified (p = 0.0104). Conclusions Percutaneous CT guided biopsy performed in patients with a history of indolent lymphoma had high accuracy in establishing the correct diagnosis regarding transformation, relapse, duplicate malignancy or a benign process. Simultaneously, the complication rate was low.","PeriodicalId":190584,"journal":{"name":"Archives of Medical Science : AMS","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115073461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Introduction of complete mesocolic excision with central vascular ligation as standardized surgical treatment for colon cancer in Greece. Results of a pilot study and bi-institutional cooperation 在希腊，采用全结肠肠系膜切除联合中央血管结扎作为结肠癌的标准化手术治疗。试点研究和双机构合作的结果

Archives of Medical Science : AMS Pub Date : 2018-11-29 DOI: 10.5114/aoms.2018.80040

A. Perrakis, N. Vassos, K. Weber, K. Matzel, K. Papadopoulos, G. Koukis, E. Perrakis, R. Croner, W. Hohenberger

{"title":"Introduction of complete mesocolic excision with central vascular ligation as standardized surgical treatment for colon cancer in Greece. Results of a pilot study and bi-institutional cooperation","authors":"A. Perrakis, N. Vassos, K. Weber, K. Matzel, K. Papadopoulos, G. Koukis, E. Perrakis, R. Croner, W. Hohenberger","doi":"10.5114/aoms.2018.80040","DOIUrl":"https://doi.org/10.5114/aoms.2018.80040","url":null,"abstract":"Introduction Complete mesocolic excision (CME) is generally accepted as state of the art in colon cancer surgery. However, the long-term impact of CME has not been systematically examined. Therefore cohort studies might be a possible way to clarify any differences between conventional resections and CME. Following bilateral cooperation between the Department of Surgery/University Hospital of Erlangen and the 1st Surgical Department of the General Hospital of Nikaia/Piraeus, including teaching activities for introduction of CME, a cohort study was performed, considering surgical quality criteria and clinical outcome. Material and methods All patients with colon carcinomas (CME group, n = 31) referred to the 1st Surgical Department of General Hospital, Nikaia/Piraeus, Greece for surgery from January 2012 to December 2013 were prospectively analyzed and compared with patients who underwent conventional surgery for colon cancer between January 2008 and December 2011 (non-CME group, n = 35). Patients’ follow-up was at least 48 months. Results There were significantly better results in terms of lymph node yield (CME group: 29.6 vs. non-CME group: 17.85; p < 0.001) and lymph node ratio (LNR) (CME group: 0.12 vs. non-CME group: 0.24; p < 0.001) and recurrence-free survival in favor of the CME group (CME group: n = 0 vs. non-CME group: n = 5) without any increase in surgical morbidity (CME group: n = 6 vs. non-CME group: n = 11; p = 0.10). Conclusions Complete mesocolic excision appears to offer a superior oncological result without any increase of postoperative morbidity and mortality. Furthermore, CME represents a surgical technique which can be established in a surgical department after previous teaching without increasing the postoperative complication rate.","PeriodicalId":190584,"journal":{"name":"Archives of Medical Science : AMS","volume":"333 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123557786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Effects of blood pressure goals on cardiovascular outcomes in hypertensive patients 血压目标对高血压患者心血管结局的影响

Archives of Medical Science : AMS Pub Date : 2018-11-29 DOI: 10.5114/aoms.2018.80013

Zonglei Du, W. Bian, Shanxia Wu, Bi-hu Gao, Yanfang Sun, Z. Kang, Xianchao Zhang

{"title":"Effects of blood pressure goals on cardiovascular outcomes in hypertensive patients","authors":"Zonglei Du, W. Bian, Shanxia Wu, Bi-hu Gao, Yanfang Sun, Z. Kang, Xianchao Zhang","doi":"10.5114/aoms.2018.80013","DOIUrl":"https://doi.org/10.5114/aoms.2018.80013","url":null,"abstract":"Introduction The aim of the study was to evaluate the effects of blood pressure (BP) goals on cardiovascular outcomes in hypertensive patients. Material and methods Primary hypertensive patients were retrospectively enrolled from outpatient clinics. The demographics, comorbidities, laboratory parameters and glomerular filtration rate (GFR) were collected. All participants were followed for 1 year. Cardiovascular outcomes included composite of all-cause mortality, non-fatal myocardial infarction, and non-fatal ischemic stroke/transient ischemic attack. Adverse event was defined as falling down and GFR decrease at follow-up. Results A total of 1226 patients were included. Based on therapeutic BP goals, participants were divided into low (< 130/80 mm Hg) and high (< 140/90 mm Hg) therapeutic goal groups and an uncontrolled hypertension (≥ 140/90 mm Hg) group. Compared to the low therapeutic goal group, patients in the uncontrolled group were older and more likely to be smokers, have longer duration of hypertension, diabetes mellitus, lower GFR and higher prevalent ischemic stroke (p < 0.05). Patients in the uncontrolled hypertension group had higher incidence of composite endpoints than low and high therapeutic goal groups. Two cases of falling down were observed in the low therapeutic goal group and no significant changes in GFR were observed. With adjustment for confounding factors, the uncontrolled hypertension group had higher risk of composite endpoints compared to low and high therapeutic goal groups, and these benefits were more prominent in the low versus high therapeutic goal group. Conclusions In hypertension patients, when compared to uncontrolled hypertension patients, low therapeutic BP goal is associated with better cardiovascular outcomes than high therapeutic BP goal.","PeriodicalId":190584,"journal":{"name":"Archives of Medical Science : AMS","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115484704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Genes and metabolic pathway of sarcoidosis: identification of key players and risk modifiers 结节病的基因和代谢途径:关键因素和危险修饰因子的鉴定

Archives of Medical Science : AMS Pub Date : 2018-11-23 DOI: 10.5114/aoms.2018.79682

M. Stjepanović, V. Mihailović-Vučinić, Vesna Spasovski, J. Milin-Lazović, V. Škodrić-Trifunović, S. Stanković, Marina Andjelković, J. Komazec, A. Momčilović, Milena Šantrić-Milićević, S. Pavlovic

{"title":"Genes and metabolic pathway of sarcoidosis: identification of key players and risk modifiers","authors":"M. Stjepanović, V. Mihailović-Vučinić, Vesna Spasovski, J. Milin-Lazović, V. Škodrić-Trifunović, S. Stanković, Marina Andjelković, J. Komazec, A. Momčilović, Milena Šantrić-Milićević, S. Pavlovic","doi":"10.5114/aoms.2018.79682","DOIUrl":"https://doi.org/10.5114/aoms.2018.79682","url":null,"abstract":"Introduction Sarcoidosis is a rare multisystem granulomatous disease with unknown etiology. The interplay of vitamin D deficiency and genetic polymorphisms in genes coding for the proteins relevant for metabolism of vitamin D is an important, but unexplored area. The aim of this study was to investigate the association between single nucleotide polymorphisms (SNPs) in CYP2R1 (rs10741657), CYP27B1 (rs10877012), DBP (rs7041; rs4588), and VDR (rs2228570) genes and sarcoidosis, as well as the association between these SNPs and 25(OH)D levels in sarcoidosis patients. Material and methods For that purpose we genotyped 86 sarcoidosis patients and 50 healthy controls using the PCR-RFLP method. Results Subjects carrying the CC genotype of CYP27B1 rs10877012 have 10 times lower odds of suffering from sarcoidosis. Moreover, DBP rs4588 AA genotype was shown to be a susceptibility factor, where carriers of this genotype had eight times higher odds for developing sarcoidosis. In addition, the A allele of the DBP gene (rs4588) was associated with lower levels of 25(OH)D in sarcoidosis patients. Conclusions These results suggest that patients with vitamin D deficiency should be regularly tested for genetic modifiers that are related to sarcoidosis in order to prevent development of serious forms of sarcoidosis.","PeriodicalId":190584,"journal":{"name":"Archives of Medical Science : AMS","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116056906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Modulator role of infliximab and methotrexate through the transient receptor potential melastatin 2 (TRPM2) channel in neutrophils of patients with rheumatoid arthritis: a pilot study 英夫利昔单抗和甲氨蝶呤通过短暂受体电位美拉他汀2 (TRPM2)通道在类风湿关节炎患者中性粒细胞中的调节作用:一项初步研究

Archives of Medical Science : AMS Pub Date : 2018-11-19 DOI: 10.5114/aoms.2018.79485

A. Doğru, M. Nazıroğlu, B. Çiğ

{"title":"Modulator role of infliximab and methotrexate through the transient receptor potential melastatin 2 (TRPM2) channel in neutrophils of patients with rheumatoid arthritis: a pilot study","authors":"A. Doğru, M. Nazıroğlu, B. Çiğ","doi":"10.5114/aoms.2018.79485","DOIUrl":"https://doi.org/10.5114/aoms.2018.79485","url":null,"abstract":"Introduction Rheumatoid arthritis (RA) is a chronic, systemic, inflammatory disease causing symmetric polyarthritis. In this study, we aimed to investigate the effects of infliximab (INF) and methotrexate (MTX) on apoptosis, oxidative stress, and calcium signaling in the neutrophils of RA patients. Material and methods Neutrophils were isolated from 10 patients with newly diagnosed RA and 10 healthy controls. They were divided into four groups (control, RA, RA + MTX, RA + INF) and incubated with MTX and INF. In the cell viability (MTT) test, the ideal non-toxic dose and incubation time of MTX were found to be 0.1 mM and 1 h, respectively. The neutrophils were also incubated with the TRPM2 channel blocker N-(p-amylcinnamoyl) anthranilic acid (ACA). Results Intracellular free Ca2+ concentration, intracellular reactive oxygen species (ROS) production, mitochondrial depolarization, lipid peroxidation, apoptosis, and caspase 3 and caspase 9 activities were found to be significantly higher in the neutrophils of RA patients compared to controls. MTT, reduced glutathione (GSH) level, and glutathione peroxidase (GSHPx) activity were significantly lower in the neutrophils of RA patients. However, MTT, GSH and GSHPx values were detected to be significantly increased with INF and MTX therapies. The Ca2+ concentrations were further decreased by the ACA therapy. Conclusions Our results suggest that INF and MTX are useful antagonists in apoptosis and mitochondrial oxidative stress in the neutrophils of RA patients. INF and MTX decreased the Ca2+ concentration through inhibition of the TRPM2 channel in the neutrophils of RA patients. It may be a new pathway in the mechanisms of anti-rheumatic drugs.","PeriodicalId":190584,"journal":{"name":"Archives of Medical Science : AMS","volume":"106 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128195331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Adiponectin improves diabetic nephropathy by inhibiting necrotic apoptosis 脂联素通过抑制坏死细胞凋亡改善糖尿病肾病

Archives of Medical Science : AMS Pub Date : 2018-11-13 DOI: 10.5114/aoms.2018.79570

W. Yi, Ouyang Qian

{"title":"Adiponectin improves diabetic nephropathy by inhibiting necrotic apoptosis","authors":"W. Yi, Ouyang Qian","doi":"10.5114/aoms.2018.79570","DOIUrl":"https://doi.org/10.5114/aoms.2018.79570","url":null,"abstract":"Introduction This study aimed to investigate the effect of adiponectin (Apn) on necrotic apoptosis (Nec) in vitro and in vivo to clarify the possible role of Apn in the pathogenesis of diabetic nephropathy (DN). Material and methods Rat glomerular endothelial (RGE) cells were treated with high glucose (HG, 30 mmol/l) for 24 h and the effects of Apn on cell viability, RIP1 and RIP3 expression and p-p38MAPK activation were assayed by CCK-8, immunofluorescence and western blot. Then a streptozotocin (STZ)-induced DN rat model was established. The body weight, left kidney weight, left kidney weight/body weight (KW/BW), creatinine clearance rate (Ccr), 24 h urine protein and blood glucose were recorded. The expression of RIP1, RIP3 and p-p38MAPK in renal tissues was examined by immunohistochemistry and western blot. Results Treatment of RGE cells with HG induced significant cytotoxicity and increased expression levels of RIP1, RIP3 and p-p38MAPK, which were abrogated by Apn in a concentration-dependent manner. In vivo, compared with the control group, the Ccr, 24 h urine protein and the blood glucose level of the rats in the model group were significantly increased, effects which were abrogated by Apn intervention. Moreover, the expression levels of RIP1, PIP3 and p-p38MAPK were also significantly increased in the model group, effects which were canceled by Apn intervention. Conclusions Apn can alleviate the inflammatory response and damage of DN by inhibiting Nec via p-p38MAPK signaling.","PeriodicalId":190584,"journal":{"name":"Archives of Medical Science : AMS","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121037154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Association of retinol binding protein-4, cystatin C, homocysteine and high-sensitivity C-reactive protein levels in patients with newly diagnosed type 2 diabetes mellitus 新诊断的2型糖尿病患者视黄醇结合蛋白-4、胱抑素C、同型半胱氨酸和高敏C反应蛋白水平的相关性

Archives of Medical Science : AMS Pub Date : 2018-11-13 DOI: 10.5114/aoms.2018.79565

Yunsheng Wang, Jun Ye, Xiao Yang, Gui-Ping Zhang, Yonghong Cao, Rong Zhang, Wu Dai, Qiu Zhang

{"title":"Association of retinol binding protein-4, cystatin C, homocysteine and high-sensitivity C-reactive protein levels in patients with newly diagnosed type 2 diabetes mellitus","authors":"Yunsheng Wang, Jun Ye, Xiao Yang, Gui-Ping Zhang, Yonghong Cao, Rong Zhang, Wu Dai, Qiu Zhang","doi":"10.5114/aoms.2018.79565","DOIUrl":"https://doi.org/10.5114/aoms.2018.79565","url":null,"abstract":"Introduction To investigate the serum retinol binding protein (RBP)-4, cystatin C (Cys C), homocysteine (HCY) and high-sensitivity C-reactive protein (hs-CRP) levels in newly diagnosed type 2 diabetes mellitus (NT2DM) patients, prediabetes mellitus (PDM) subjects and normal controls, as well as their correlation with clinical and laboratory indexes, such as blood pressure and lipoprotein. Material and methods A total of 242 subjects, including 141 NT2DM patients, 48 PDM subjects and 53 healthy controls, were recruited in the present study. Serum RBP-4, Cys C and hs-CRP concentrations were measured by enzyme-linked immunosorbent assay (ELISA). HCY concentration was determined by the chemical luminescence method. Results There were significant differences in Cys C and hs-CRP among NT2DM patients, PDM subjects and normal controls. In comparison to controls, there were significantly elevated Cys C and hs-CRP levels in PDM (both p < 0.001), and a significantly increased Cys C level in NT2DM (p < 0.001); however, there were no significant differences in Cys C and hs-CRP levels between NT2DM and PDM, and no significant differences of hs-CRP levels between NT2DM and normal controls. No significant differences of RBP-4 and HCY levels among NT2DM, PDM and normal control groups were observed. Conclusions Aberrant Cys C expression and its clinical associations in NT2DM suggest their important role in this disease.","PeriodicalId":190584,"journal":{"name":"Archives of Medical Science : AMS","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130431191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Clinicopathological factors associated with novel prognostic markers for patients with triple negative breast cancer 与三阴性乳腺癌患者新型预后标志物相关的临床病理因素

Archives of Medical Science : AMS Pub Date : 2018-11-13 DOI: 10.5114/aoms.2018.79568

A. Badowska-Kozakiewicz, M. Budzik, Anna Liszcz, M. Sobieraj, A. Czerw, M. Sobol, J. Patera, A. Deptała

{"title":"Clinicopathological factors associated with novel prognostic markers for patients with triple negative breast cancer","authors":"A. Badowska-Kozakiewicz, M. Budzik, Anna Liszcz, M. Sobieraj, A. Czerw, M. Sobol, J. Patera, A. Deptała","doi":"10.5114/aoms.2018.79568","DOIUrl":"https://doi.org/10.5114/aoms.2018.79568","url":null,"abstract":"Introduction Triple negative breast cancer (TNBC) is characterized by a worse prognosis than other breast cancer subtypes. TNBC is defined by lack of expression of estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2. The aim of this analysis was to evaluate the relationship between immunohistochemical expression of novel prognostic markers (erythropoietin (EPO) and erythropoietin receptor (EPO-R)) and clinicopathological features of TNBC and non-TNBC patients. Material and methods Our analysis was conducted on a group of 162 patients with breast carcinoma with lymph node metastasis (111 TNBC and 51 non-TNBC). All statistical analyses were performed with SPSS software v 12.0. Results Histopathologic subtyping of the 111 triple negative breast cancers identified 89.1% invasive ductal carcinomas of no special type and 10.9% other special types of cancers. TNBC more often presented EPO-R and EPO expression (36%; 37.8%) than non-TNBC (23.5%; 29.4%). Non-TNBC subgroup showed statistically significant correlation only between Ki-67 expression and histological grade (G1-G3) (p < 0.001), while TNBC subgroup demonstrated significant correlation between Ki-67 expression and histological grade (G1-G3) and tumor size (pT1-pT4) as well (p = 0.002; p = 0.042), between the EPO-R expression and histological grade (G1-G3) (p < 0.001). Conclusions The relationship between the expression of EPO-R and histological malignancy grade in triple negative breast cancer, suggests that the present EPO-R expression in TNBC may constitute an additional prognostic factor.","PeriodicalId":190584,"journal":{"name":"Archives of Medical Science : AMS","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115760194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Inhibition of protein kinase C activity inhibits osteosarcoma metastasis 抑制蛋白激酶C活性可抑制骨肉瘤转移

Archives of Medical Science : AMS Pub Date : 2018-11-07 DOI: 10.5114/aoms.2018.79450

Hejun Hu, Xiong-wei Deng, Runpeng Li, Debra Chen, Chao Xue

{"title":"Inhibition of protein kinase C activity inhibits osteosarcoma metastasis","authors":"Hejun Hu, Xiong-wei Deng, Runpeng Li, Debra Chen, Chao Xue","doi":"10.5114/aoms.2018.79450","DOIUrl":"https://doi.org/10.5114/aoms.2018.79450","url":null,"abstract":"Introduction For some cancers bone is the preferred site for metastasis and involves a cascade involving transition of epithelial cells to mesenchymal cells and subsequent intravasation to the blood and lymph vessels, and finally hematogenous dissemination to perivascular niches of the bone marrow sinusoids. It has been shown that protein kinase C can aid metastasis to bone. Hence, pharmacological inhibition of protein kinase C (PKC) activity is thought of as a potential therapeutic option in bone metastatic lesions. The objective of the current study was to investigate how PKCs exert their effect on bone cancer metastasis and to test the efficacy of pharmacological inhibition of PKC on bone metastasis. Material and methods The effect of the PKC inhibitor Go6983 on epithelial and mesenchymal cell marker expression in the osteosarcoma cell line DAN was determined by immunoblot and immunofluorescence analysis. The in vivo effect of Go6983 was evaluated with a xenograft model using DAN cells. Results Treatment with transforming growth factor β (TGF-β) led to loss of the epithelial cell marker and gain of mesenchymal cell markers in the osteosarcoma cell line, DAN. This transition occurred concomitantly with PKC activation. TGF-β-mediated PKC activation resulted in activation of ribosomal protein 6 (S6), but not S6K1. Pharmacological inhibition of PKC activation attenuated these effects. In a xenograft model of experimental metastasis, pharmacological inhibition of PKC activation over a period of 4 weeks reduced both tumor burden and metastasis to lungs. Conclusions Our results indicate that PKC potentiates tumor metastasis to the bone by potentiating translation increase and can be putatively inhibited by pharmacological inhibition.","PeriodicalId":190584,"journal":{"name":"Archives of Medical Science : AMS","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122184393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Association of lipoprotein(a) and major adverse cardiovascular events in patients with percutaneous coronary intervention 经皮冠状动脉介入治疗患者的脂蛋白(a)与主要不良心血管事件的关系

Archives of Medical Science : AMS Pub Date : 2018-11-06 DOI: 10.5114/aoms.2018.79401

Zhihao Chen, Chaohui Jiang, H. Qu, Shuang Liang, Jian Yang, Hui Wu, Chao He, Xin-an Wang

{"title":"Association of lipoprotein(a) and major adverse cardiovascular events in patients with percutaneous coronary intervention","authors":"Zhihao Chen, Chaohui Jiang, H. Qu, Shuang Liang, Jian Yang, Hui Wu, Chao He, Xin-an Wang","doi":"10.5114/aoms.2018.79401","DOIUrl":"https://doi.org/10.5114/aoms.2018.79401","url":null,"abstract":"Introduction The aim of the current study was to evaluate the association between lipoprotein(a) [Lp(a)] and major adverse cardiovascular events (MACEs) in patients with percutaneous coronary intervention (PCI) treatment. Material and methods This was a retrospective study. The demographics, prior medical histories, comorbidities and laboratory parameters were collected from the electronic health record. All participants were followed up for 1 year after the indexed PCI. Studied end points were a composite of MACEs including all-cause mortality, non-fatal myocardial infarction (MI), non-fatal ischemic stroke, transient ischemic attack and stent restenosis. Results During 1-year follow-up, 87 MACEs occurred. Compared to patients who did not have MACEs, patients who had MACEs were older, more likely to have higher body mass index, diabetes mellitus and left main lesion, and also had higher baseline low density lipoprotein cholesterol (LDL-C) and Lp(a) levels. All patients in both groups were prescribed aspirin and clopidogrel at discharge. Nearly 97.4% and 95.4% of patients in both groups were treated with statins and a higher proportion of patients in the MACE group were treated with ezetimibe (11.5% vs. 3.5%, p < 0.05). In multivariate regression analysis, diabetes mellitus, LDL-C, Lp(a) and glomerular filtration rate were independent risk factors for MACEs; statin use appeared to be a protective factor for MACEs. Patients with increased Lp(a) level had significantly higher incidence of MACEs than the normal Lp(a) level group (p = 0.001). Conclusions Baseline serum Lp(a) can be used to predict MACEs in patients after PCI treatment, which was independent of LDL-C.","PeriodicalId":190584,"journal":{"name":"Archives of Medical Science : AMS","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130040801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9