Molecular Genetics, Microbiology and Virology最新文献

{"title":"The Effect of the flhB Plasmid Gene of the Flagellar Export Component on Flagellation and Motility in Azospirillum Bacteria","authors":"","doi":"10.3103/s0891416823030060","DOIUrl":"https://doi.org/10.3103/s0891416823030060","url":null,"abstract":"<h3>Abstract</h3> <p>The effect was analyzed of the <em>flhB2</em> gene, which is located on the AZOBR_p4 (AZOBR_p410073 gene) plasmid in <em>Azospirillum baldaniorum</em> Sp245 and on the ABSP7_p3 (AMK58_26270 gene) plasmid in <em>A. brasilense</em> Sp7 and codes for the FlhB protein, a flagellar export component that ensures flagellin assembly, flagellation, and motility in these bacteria. We used <em>A. baldaniorum</em> strain Sp245, its Fla^– Laf^– mutant Sp245.1063 (Sp245-<em>flhB1</em>::Omegon-Km), and <em>A. brasilense</em> Sp7. Mutants defective in the <em>flhB2</em> gene were generated by site-directed mutagenesis. Bacterial morphology and motility were characterized by electron and phase-contrast microscopy. An <em>A. baldaniorum</em> Sp245 mutant, Sp245-<em>flhB2</em>::Km, was generated that had a cloned kanamycin resistance gene in the coding sequence (CDS) AZOBR_p410073. In contrast to the Fla^– Laf^– mutant Sp245-<em>flhB1</em>::Omegon-Km, the <em>flhB1</em> chromosomal gene of which is inactivated (AZOBR_150177 gene), strain Sp245-<em>flhB2</em>::Km retained the synthesis of a functioning polar flagellum (Fla), but the synthesis and functioning of lateral flagella (Laf) was impaired and the movement and spreading rates of swarming cells in semiliquid agarized media were decreased. Inactivation of the AMK58_26270 plasmid gene in Sp7, which is homologous to the AZOBR_p410073 gene (97% identity), resulted in a similar Laf^– phenotype in the corresponding mutant. Two putative <em>flhB</em> genes are present in the genome of strains Sp245 and Sp7. These genes are located in the chromosome (<em>flhB1</em>) and on the AZOBR_p4 or ABSP7_p3 plasmid (<em>flhB2</em>), respectively. Expression of the <em>flhB2</em> gene is required for Laf assembly. Transcription of <em>flhB2</em> is regulated by a mechanosignal, the perception and generation of which is ensured by the functioning Fla, apparently with the involvement of the FlhB protein encoded by the <em>flhB1</em> chromosomal gene.</p>","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139463275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benefits of Using the CRISPR/Cas9 System for the Correction of Genetic Mutations","authors":"","doi":"10.3103/s0891416823030084","DOIUrl":"https://doi.org/10.3103/s0891416823030084","url":null,"abstract":"<h3>Abstract</h3> <p>The discovery of the CRISPR/Cas9 gene scissors technology was a breakthrough in the development of gene therapy methods and attracted the attention of the entire scientific community. This technology provides prospects for the treatment of many neurodegenerative diseases and cancers that were previously considered almost incurable. To date, the mechanisms of the development and progression of these groups of nosologies are becoming increasingly studied due to discoveries in the field of molecular biology and genetics. However, the application of the CRISPR/Cas9 technology has some limitations and difficulties that should be considered. The development of works on the creation and selection of specific gene carriers, a thorough safety assessment of modern genetic manipulations, and the integrated participation of specialists from different fields of science will be important to solve the problems associated with the use of the CRISPR/Cas9 technology and achieve the desired therapeutic effect.</p>","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"30 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139463078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hantaviruses in Populations of Voles in Siberia","authors":"L. N. Yashina, S. A. Abramov, N. A. Smetannikova, B. S. Malyshev, T. A. Dupal, A. V. Krivopalov","doi":"10.3103/s0891416823020106","DOIUrl":"https://doi.org/10.3103/s0891416823020106","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135143261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus crispatus and Human Herpes Simplex Virus-2, an In Vitro Study","authors":"Mehdi Moazzami Goudarzi, Mohammad Reza Fazeli, Abbas Akhavan Sepahi, Akram Eidi","doi":"10.3103/s0891416823020040","DOIUrl":"https://doi.org/10.3103/s0891416823020040","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"193 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135143255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant RBD of the SARS-CoV-2 Spike Protein: Production in Escherichia coli Cells, Binding to Antibodies, and Antiviral Activity","authors":"M. S. Gromova, A. V. Gromov, T. M. Grunina, A. M. Lyashchuk, Z. M. Galushkina, M. E. Subbotina, I. B. Esmagambetov, E. I. Ryabova, V. V. Prokofiev, A. V. Kovyrshina, A. A. Ilyukhina, A. Y. Shelkov, A. S. Karyagina, V. G. Lunin","doi":"10.3103/s0891416823020052","DOIUrl":"https://doi.org/10.3103/s0891416823020052","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135143623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition of Populations and Durations of Persistence of Bordetella pertussis Bacteria in Patients with Whooping Cough and Contact Persons","authors":"A. Yu. Medkova, E. G. Semin, S. V. Kulikov, Yu. V. Nesterova, I. V. Babachenko, L. N. Sinyashina, G. I. Karataev","doi":"10.3103/s0891416823020064","DOIUrl":"https://doi.org/10.3103/s0891416823020064","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135142733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monoclonal Antibody Panel for Hepatitis B Virus Genotype and HBsAg Subtype Identification: Results Obtained for Blood-Serum Samples from Three Regions of Russia","authors":"L. V. Bezuglova, E. I. Sergeeva, L. Yu. Voevodskaya, E. E. Lysykh, N. V. Polovitsa, I. V. Deliy, M. K. Ivanov, I. G. Netesova, S. V. Netesov","doi":"10.3103/s0891416823020039","DOIUrl":"https://doi.org/10.3103/s0891416823020039","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135143257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA N6-Methyladenosine Pathway Writer Genes Expression Levels and Clinical Severity of Infection in Covid-19 Patients","authors":"Badel Arslan, Sevgi Baltacı, Burcu Bayyurt, Nil Özbilum Sahin, Murat Eser Akyurek, Mehmet Bakır, Serdal Arslan","doi":"10.3103/s0891416823020118","DOIUrl":"https://doi.org/10.3103/s0891416823020118","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135143263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Mutations in gyrA Gene with Resistance to Fluoroquinolones in Clinical Isolates of Multidrug-Resistant Mycobacterium tuberculosis","authors":"Hanieh Bagherifard, Mitra Salehi, Mona Ghazi","doi":"10.3103/s0891416823020027","DOIUrl":"https://doi.org/10.3103/s0891416823020027","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135143253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LZTFL1 rs17713054 Polymorphism as an Indicator Allele for COVID-19 Severity","authors":"Mona Roozbehani, Hossein Keyvani, MohammadHossein Razizadeh, Parastoo Yousefi, Ali Gholami, Alireza Tabibzadeh, Leila Mousavizadeh","doi":"10.3103/s0891416823020088","DOIUrl":"https://doi.org/10.3103/s0891416823020088","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135142735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}