Nematologica最新文献_第6页

RFLP analysis in Canary Islands and north European populations of potato cyst nematodes (Globodera spp.): hybridization with cloned fragments. 加那利群岛和北欧马铃薯囊线虫种群的RFLP分析:与克隆片段杂交。

Nematologica Pub Date : 1997-01-01 DOI: 10.1163/004825997X00033

J. A. González, M. Phillips, D. Trudgill

{"title":"RFLP analysis in Canary Islands and north European populations of potato cyst nematodes (Globodera spp.): hybridization with cloned fragments.","authors":"J. A. González, M. Phillips, D. Trudgill","doi":"10.1163/004825997X00033","DOIUrl":"https://doi.org/10.1163/004825997X00033","url":null,"abstract":"Four random probes from a Globodera pallida DNA library and one rDNA repeat probe from flax were used to highlight specific fragments in total DNA restricted with the endonucleases Bam HI, Eco RI, Eco RV, and Hind III, from 26 potato cyst nematode (PCN) populations (G. rostochiensis and G. pallida). The percentage similarity between species was low (%sm = 45.6). Cluster and principal coordinate analysis allowed us to hypothesise about the epidemiology of these populations. In the G. rostochiensis group, the populations representing pathotypes Ro2, Ro3, Ro4 and Ro5 may have been derived from the same introduction. Canarian populations R1, R2, R6, R7, and R10 were distinct from all the controls but R4 and R5 were close to the British Ro1 population. In the G. pallida group, the Luffness population was distinct from all others suggesting that it may represent a distinct introduction of PCN into the British Isles. Canarian population P9 had ten markers which were absent from the European control populations and it is possible that this population represents a distinct introduction of G. pallida into the Island of Tenerife.","PeriodicalId":18988,"journal":{"name":"Nematologica","volume":"140 1","pages":"157-172"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87546086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Four New Nematode Species From the Bothnian Bay, Northern Baltic Sea, With a Redescription of Microlaimus Globiceps De Man, 1880 (Nematoda) 波罗的海北部波斯尼安湾线虫四新种，并附1880年Globiceps De Man线虫的再描述(线虫纲)

Nematologica Pub Date : 1997-01-01 DOI: 10.1163/004725997X00034

T. Turpeenniemi

{"title":"Four New Nematode Species From the Bothnian Bay, Northern Baltic Sea, With a Redescription of Microlaimus Globiceps De Man, 1880 (Nematoda)","authors":"T. Turpeenniemi","doi":"10.1163/004725997X00034","DOIUrl":"https://doi.org/10.1163/004725997X00034","url":null,"abstract":"Four free-living nematodes from the Bothnian Bay, the northern part of the Baltic Sea, are described as new to science. A key to the genus Eleutherolaimus Filipjev, 1922, is given. Eleutherolaimus schneideri sp. n. is characterized by unequal cephalic setae and subcephalic setae, subcephalic setae shorter than the head diameter, amphid position in junction of buccal cavity and oesophagus, long cardia and short spicules. Microlaimus limnophilus sp. n. belongs to the group of small microlaimids (shorter than 0.5 mm) and is characterized by very small teeth (dorsal tooth about 0.5 μm long) and wide body annules (2.5-3.0 μm in midbody). Prismatolaimus paraprimitivus sp. n. has a slender body (a = 56-65), outer labial setae and cephalic setae in separate circles, wide amphid (2/3 of corresponding body diameter), precloacal supplements confined to posterior body region and long tail. Halalaimus dimorphus sp. n. is recognized by long amphid, posteriorly narrow oesophagus, setose inner labial sense organs, well-separated head setae, swollen, blunt tail end. The reproductive system of Halalaimus dimorphus sp. n. is unique in showing both sperm and egg dimorphism; the spermatozoa have longitudinal striations. Microlaimus globiceps de Man, 1880 is redescribed.","PeriodicalId":18988,"journal":{"name":"Nematologica","volume":"6 1","pages":"31-58"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89763457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Serological identification and quantification of Heterodera avenae from processed soil samples 处理过的土壤样品中中华异线虫的血清学鉴定与定量

Nematologica Pub Date : 1997-01-01 DOI: 10.1163/004825997X00079

R. Curtis, M. Al-Hinai, A. E. R. Diggines, K. Evans

{"title":"Serological identification and quantification of Heterodera avenae from processed soil samples","authors":"R. Curtis, M. Al-Hinai, A. E. R. Diggines, K. Evans","doi":"10.1163/004825997X00079","DOIUrl":"https://doi.org/10.1163/004825997X00079","url":null,"abstract":"Monoclonal antibodies (MAbs) and polyclonal antibodies (PCs) were produced to antigens of an Australian population of cereal cyst nematode (CCN), Heterodera avenae. MAb IACR CCNj-49.2 recognised an antigen with a molecular weight of approximately 200 kDa, which was immunolocalised apparently in granules in the nematode gut. A procedure to extract CCN antigens from soil samples, under laboratory conditions was devised, and 50 g samples of soil containing 5 CCN cysts were processed by two sets of flotation techniques to recover the nematodes. Milling using Ballotini glass beads was then used to release the antigens from the CCN cysts recovered in the float. A double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) was the better ELISA format used to identify and quantify the CCN population from the processed soil samples containing up to 20% of organic matter. The threshold limit of detection was estimated by serial dilutions of the soil extracts. It was approximately equivalent to 0.5 eggs or 3.6 eggs per g of soil in a DAS-ELISA, when using respectively the PC or the MAb as the trapping antibody. The assay could be made more sensitive in soils with lower contents of organic matter.","PeriodicalId":18988,"journal":{"name":"Nematologica","volume":"36 1","pages":"199-213"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81216672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Report of the ectoparasitic nematode Noctuidonema (Acugutturidae) infesting Lepidoptera in the Fiji Islands.3) 斐济群岛鳞翅目外寄生线虫夜蛾(夜蛾科)的报告。3)

Nematologica Pub Date : 1997-01-01 DOI: 10.1163/005125997X00093

C. E. Rogers, O. G. Marti, J. Clayton

引用次数: 2

New Records and Descriptions of Plant Nematodes From Rain Forest Soil in French Guiana, With a First Description of Males of Mesocriconema Surinamense (De Grisse & Maas, 1970) Loof & De Grisse, 1989 (Tylenchida: Nemata) 法属圭亚那热带雨林土壤植物线虫的新记录与描述，包括苏里纳Mesocriconema suinamense (De Grisse & Maas, 1970)的首次雄性描述(Loof & De Grisse, 1989)

Nematologica Pub Date : 1997-01-01 DOI: 10.1163/005025997X00085

E. Berg, P. Quénéhervé, L. Tiedt

引用次数: 2

Comments on some known species of the genus Tylenchus and description of Tylenchus stachys sp. n. (Nematoda:Tylenchidae). 文章题目滇蝗属若干已知种的评述及滇蝗科滇蝗属描述。

Nematologica Pub Date : 1996-01-01 DOI: 10.1163/004525996X00019

M. Brzeski