Nahrung-foodPub Date : 1998-04-01DOI: 10.1002/(SICI)1521-3803(199804)42:02<68::AID-FOOD68>3.0.CO;2-#
Y. Shu, Soichiro Nakamura, A. Kato
{"title":"The role of polysaccharide-chain attachment to lysozyme in the excellent emulsifying properties of polymannosyl lysozyme","authors":"Y. Shu, Soichiro Nakamura, A. Kato","doi":"10.1002/(SICI)1521-3803(199804)42:02<68::AID-FOOD68>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1521-3803(199804)42:02<68::AID-FOOD68>3.0.CO;2-#","url":null,"abstract":"Polymannosyl lysozyme (G49N), which is polymannosylated at the position 49 by genetic modification, exhibited excellent emulsifying properties at low concentrations compared with a commercial emulsifier, Sunsoft SE-16. The polymannosyl chain was removed from G49N by the treatment with hydrazine anhydrous without any degradation of the chain. The removal of polymannosyl chain resulted in a dramatic decrease in the emulsifying properties, while the mixture of lysozyme and the polymannosyl chain did not exhibited good emulsifying properties. This suggests that the polysaccharide-chain attachment to lysozyme is critical for the excellent emulsifying properties of polymannosyl lysozyme.","PeriodicalId":18955,"journal":{"name":"Nahrung-food","volume":"45 1","pages":"68-70"},"PeriodicalIF":0.0,"publicationDate":"1998-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87723676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nahrung-foodPub Date : 1998-04-01DOI: 10.1002/(SICI)1521-3803(199804)42:02<61::AID-FOOD61>3.3.CO;2-W
M. Vorob'ev, I. A. Goncharova
{"title":"Computer simulation of proteolysis. Peptic hydrolysis of partially demasked β-Lactoglobulin","authors":"M. Vorob'ev, I. A. Goncharova","doi":"10.1002/(SICI)1521-3803(199804)42:02<61::AID-FOOD61>3.3.CO;2-W","DOIUrl":"https://doi.org/10.1002/(SICI)1521-3803(199804)42:02<61::AID-FOOD61>3.3.CO;2-W","url":null,"abstract":"Computer assistance was used for simulation of proteolysis of partially demasked β-Lactoglobulin by pepsin. Total proteolysis kinetics and kinetics for intermediate peptides were computed by PROTEOLYSIS program for different rates of demasking. The increase of demasking rate by a factor of 100 gives twofold increase in the total hydrolysis rate. The identification of cleavage sites on the basis of limited number of peptides was found to depend on the depth of hydrolysis. When the number of identified peptides was only 10, the computer simulation has allowed to predict a half of experimentally determined cleavage sites. The influence of the dimension of enzyme active site and of the secondary structure of substrate on the efficiency of prediction of cleavage sites was analysed.","PeriodicalId":18955,"journal":{"name":"Nahrung-food","volume":"18 1","pages":"61-67"},"PeriodicalIF":0.0,"publicationDate":"1998-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84351412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nahrung-foodPub Date : 1998-04-01DOI: 10.1002/(SICI)1521-3803(199804)42:02<59::AID-FOOD59>3.3.CO;2-N
I. Vaintraub
{"title":"Kinetics of the co‐operative proteolysis","authors":"I. Vaintraub","doi":"10.1002/(SICI)1521-3803(199804)42:02<59::AID-FOOD59>3.3.CO;2-N","DOIUrl":"https://doi.org/10.1002/(SICI)1521-3803(199804)42:02<59::AID-FOOD59>3.3.CO;2-N","url":null,"abstract":"Experiental data showing the kinetics of the co-operative proteolysis obeying to pseudo first-order reaction are compiled. An explanation of this regularity is proposed and its limitations are discussed.","PeriodicalId":18955,"journal":{"name":"Nahrung-food","volume":"235 1","pages":"59-60"},"PeriodicalIF":0.0,"publicationDate":"1998-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75909283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nahrung-foodPub Date : 1998-02-01DOI: 10.1002/(SICI)1521-3803(199802)42:01<16::AID-FOOD16>3.3.CO;2-F
O. Zorba, S. Özdemir, H. Gökalp
{"title":"Stability of model emulsions prepared using whey and muscle proteins","authors":"O. Zorba, S. Özdemir, H. Gökalp","doi":"10.1002/(SICI)1521-3803(199802)42:01<16::AID-FOOD16>3.3.CO;2-F","DOIUrl":"https://doi.org/10.1002/(SICI)1521-3803(199802)42:01<16::AID-FOOD16>3.3.CO;2-F","url":null,"abstract":"The amount of water (SW) and oil (SO) separated from model emulsions and emulsion stability (ES) of these emulsions prepared from corn oil and of fluid whey, total muscle protein (TMP), whey + TMP and sarcoplasmic protein (SP) were examined. The SW value of whey + TMP emulsions was lower (26,33%) than that of TMP (31,33%), SP (39,0%) practically the same as that of whey only. However, the SO value of whey emulsions was higher (9,40%) than that of muscle protein emulsions (0,0%). It was found that there was no oil separation in whey + TMP emulsions. Whey proteins had the lowest ES (64,6 ± 0,96) among the proteins studied. Nevertheless, whey + TMP emulsions had the highest ES (73,67 < 0,58).","PeriodicalId":18955,"journal":{"name":"Nahrung-food","volume":"3 1","pages":"16-18"},"PeriodicalIF":0.0,"publicationDate":"1998-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75903959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nahrung-foodPub Date : 1998-02-01DOI: 10.1002/(SICI)1521-3803(199802)42:01<7::AID-FOOD7>3.3.CO;2-9
M. Czarnecka, Z. Czarnecki, J. Nowak, H. Roszyk
{"title":"Effect of lactic fermentation and extrusion of bean and pea seeds on nutritional and functional properties","authors":"M. Czarnecka, Z. Czarnecki, J. Nowak, H. Roszyk","doi":"10.1002/(SICI)1521-3803(199802)42:01<7::AID-FOOD7>3.3.CO;2-9","DOIUrl":"https://doi.org/10.1002/(SICI)1521-3803(199802)42:01<7::AID-FOOD7>3.3.CO;2-9","url":null,"abstract":"Controlled lactic acid fermentation of „Bomba” bean and „Opal” pea was used to improved their nutritional and functional properties. The modified legumes were used as a source for extrusion process to obtain snack-type foods. Milled legume seeds were fermented by different population of Lactobacillus plantarum with different moisture content in plant material to estimate the influence of this two parameters on the dynamics of lactic fermentation. Quantitative and qualitative changes in carbohydrates and nitrogen compounds of modified seeds were analyzed (e.g . mono- and oligosaccharides, \"in vitro\" starch and protein digestibility). In the new snack-type products, functional properties: water solubility index (WSI), water absorption index (WAI), expansion index, bulk density and texture explained as a shearing stress were recorded. Results showed a distinct decrease in raffinose, verbascose and stachyose contents even up to 80 - 90% after fermentation and extrusion process. Lactic fermentation and extrusion of bean and pea seeds contributes to a significant (a ≤ 0,05) increase of \"in vitro\" protein and starch digestibility. Observations of changes in functional properties of extruderates (e. g., expansion, shearing stress, WSI, WAI) allowed to choose the most efficient model of fermentation pretreatment to obtain the best improvement of nutritional and functional features of the product.","PeriodicalId":18955,"journal":{"name":"Nahrung-food","volume":"173 4 1","pages":"7-11"},"PeriodicalIF":0.0,"publicationDate":"1998-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86717359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}