Genetics and molecular research : GMR最新文献_第6页

Genetic diversity of Cajazeira (Spondias mombin L.) in three geographic regions. 3个地理区域中华绒螯虾的遗传多样性。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16018946

B. M. Silva, A. Rossi, A. V. Tiago, K. Schmitt, J. F. E. Dardengo, Sérgio Alessandro Machado Souza

{"title":"Genetic diversity of Cajazeira (Spondias mombin L.) in three geographic regions.","authors":"B. M. Silva, A. Rossi, A. V. Tiago, K. Schmitt, J. F. E. Dardengo, Sérgio Alessandro Machado Souza","doi":"10.4238/gmr16018946","DOIUrl":"https://doi.org/10.4238/gmr16018946","url":null,"abstract":"Cajazeira (Spondias mombin L.), of the family Anacardiaceae, is a species of fruit tree found in the Amazon region with fruits that have excellent prospects for commercial use. We aimed to evaluate the genetic diversity within and among natural populations of S. mombin, with natural occurrence in northern Mato Grosso State, by using inter-simple sequence repeat (ISSR) markers. Overall, 126 individuals were evaluated from three populations located Alta Floresta (AFL) 42, Marcelândia (MAR) 41, and Nova Bandeirantes (NBA) 43. The individuals were genotyped with 14 ISSR primers, which amplified 99 fragments. All markers, with the exception of DiGA3'A, presented a polymorphic information content above 0.25, and thus, are recommended for diversity analyses in S. mombin. Genetic diversity of the AFL [Nei's diversity (H) = 0.2430 and Shannon index (I) = 0.3547] and MAR (H = 0.2062 and I = 0.2993) populations was higher when compared to the NBA population, which presented the lowest genetic diversity (H = 0.2002 and I = 0.2957). Analysis of molecular variance showed that 77.38% of the total genetic variation is found within populations while 22.62% is found among populations. AFL and NBA are genetically the most similar populations and also the closest \"Structure\" revealed genetic diversity among the genotypes of each population. As there is genetic variability in both populations, and there are no genetically identical individuals, both populations can be a source of genotypes for germplasm banks and for future commercial fruitful plantations S. mombin.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131801529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Analysis of specific serum markers of colon carcinoma using a Bhattacharyya-based support vector machine. 基于bhattacharya的支持向量机分析结肠癌特异性血清标志物。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019521

Wenyi Yang, G. Shi, Liping Wu, Shutang Wei, Y. Huang, Lixia Tan, R. Yang, Chunxiao Yan, E. Guo, Hangyu Wang, J. Tong, Y. Dong, Dazheng Han

引用次数: 2

Cytotoxicity evaluation of the whole protein extract from Bar-transgenic rice on Mus musculus lymphocytes. bar转基因水稻全蛋白提取物对小家鼠淋巴细胞的细胞毒性评价。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019331

X. B. Lu

{"title":"Cytotoxicity evaluation of the whole protein extract from Bar-transgenic rice on Mus musculus lymphocytes.","authors":"X. B. Lu","doi":"10.4238/gmr16019331","DOIUrl":"https://doi.org/10.4238/gmr16019331","url":null,"abstract":"With the expanding demand for genetically modified (GM) rice, its safety evaluation is of great significance. Therefore, this study was carried out to assess the acute cytotoxicity of the whole protein extract from GM rice Bar68-1 in Mus musculus lymphocytes in vitro. Cell viability was determined by Cell Counting Kit-8 (CCK-8) and Neutral Red Uptake (NRU) tests. CCK-8 tests was carried out according to the manufacturer's instructions. Cell dehydrogenase (catalytic redox enzymes) activity was spectrophotometrically determined at 450 nm. The tests result were recorded immediately. NRU tests were completed under yellow light in a dark room according to an improved protocol. Lysosomal uptake of neutral red was spectrophotometrically determined at 540 nm and the results were recorded immediately. The results showed that the survival rate of M. musculus lymphocytes in the positive control group was significantly less than in the blank control group (P < 0.05). Moreover, an exposure- time-effect relationship was observed in the positive control group with CCK-8 and NRU tests. There was no significant difference in survival rate between GM rice Bar68-1group and non-GM rice D68 group (P > 0.05). The GM rice Bar68-1 group also did not show a higher survival rate than non-GM rice D68 group (P > 0.05). These results suggested that the whole protein extract from Bar68-1 and D68 were equivalent in their cytotoxicity, and GM rice Bar68-1 had no acute cytotoxic effect on M. musculus lymphocytes in vitro.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123401802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correlation-based linear discriminant classification for gene expression data. 基于相关性的基因表达数据线性判别分类。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019357

M. Pan, J. Zhang

{"title":"Correlation-based linear discriminant classification for gene expression data.","authors":"M. Pan, J. Zhang","doi":"10.4238/gmr16019357","DOIUrl":"https://doi.org/10.4238/gmr16019357","url":null,"abstract":"Microarray gene expression technology provides a systematic approach to patient classification. However, microarray data pose a great computational challenge owing to their large dimensionality, small sample sizes, and potential correlations among genes. A recent study has shown that gene-gene correlations have a positive effect on the accuracy of classification models, in contrast to some previous results. In this study, a recently developed correlation-based classifier, the ensemble of random subspace (RS) Fisher linear discriminants (FLDs), was utilized. The impact of gene-gene correlations on the performance of this classifier and other classifiers was studied using simulated datasets and real datasets. A cross-validation framework was used to evaluate the performance of each classifier using the simulated datasets or real datasets, and misclassification rates (MRs) were computed. Using the simulated data, the average MRs of the correlation-based classifiers decreased as the correlations increased when there were more correlated genes. Using real data, the correlation-based classifiers outperformed the non-correlation-based classifiers, especially when the gene-gene correlations were high. The ensemble RS-FLD classifier is a potential state-of-the-art computational method. The correlation-based ensemble RS-FLD classifier was effective and benefited from gene-gene correlations, particularly when the correlations were high.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"153 1-4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120928572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

An assessment of the correlation between early postinfarction pyramidal tract Wallerian degeneration and nerve function recovery using diffusion tensor imaging. 弥散张量成像评价梗死后早期锥体束沃勒氏变性与神经功能恢复的相关性。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019035

A. Guo, F. Hao, L. Liu, B. Wang, X. Jiang

{"title":"An assessment of the correlation between early postinfarction pyramidal tract Wallerian degeneration and nerve function recovery using diffusion tensor imaging.","authors":"A. Guo, F. Hao, L. Liu, B. Wang, X. Jiang","doi":"10.4238/gmr16019035","DOIUrl":"https://doi.org/10.4238/gmr16019035","url":null,"abstract":"This study aimed to evaluate the clinical significance of diffusion tensor imaging (DTI) in the early diagnosis of pyramidal tract Wallerian degeneration (WD) and assessment of neurological recovery following cerebral infarction. This study included 23 patients with acute cerebral infarction and 10 healthy adult controls. All participants underwent both magnetic resonance imaging (MRI) and DTI scans. DTI images were analyzed using the Functional MRI of the Brain Software Library to determine the regions of interest (ROI) and obtain the mean diffusivity (MD) and fractional anisotropy (FA) value for each ROI. The correlation between FA or MD and postinfarction functional recovery of the nervous system was further analyzed to assess the feasibility of using a DTI scan in the evaluation of functional recovery of the nervous system in patients with cerebral infarction. DTI may be useful in detecting signals of early postinfarction pyramidal tract WD and is useful for the evaluation of postinfarction neurological recovery. Cerebral lesions were detected using MRI in all patients. It was found that in some patients, the FA value of the ipsilateral pyramidal tract on DTI was decreased as early as day 3 after the onset of infarction and in all patients by day 7. Subsequent correlation studies showed that the FA value of the ipsilateral pyramidal tract on day 13 was negatively correlated with the National Institutes of Health Stroke Scale score, but positively correlated with the Barthel Index, motricity index, and modified Rankin Scale scores.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131989996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Genetic diversity of Casearia sylvestris populations in remnants of the Atlantic Forest. 大西洋森林遗迹中西尔维斯特Casearia sylvestris种群的遗传多样性。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019105

F. L. Araujo, Marcos Vinicius B M Siqueira, C. Grando, João Paulo Gomes Viana, J. B. Pinheiro, A. Alves-Pereira, J. B. Campos, P. Brancalion, M. Zucchi

引用次数: 5

Protective role of +294 T/C (rs2016520) polymorphism of PPARD in Mexican patients with colorectal cancer. PPARD +294 T/C (rs2016520)多态性在墨西哥结直肠癌患者中的保护作用

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019324

M. A. Rosales-Reynoso, L. Wence-Chávez, A. R. Arredondo-Valdez, S. Dumois-Petersen, Patricio Barros-Núñez, M. Gallegos-Arreola, S. Flores-Martínez, José Sánchez-Corona

{"title":"Protective role of +294 T/C (rs2016520) polymorphism of PPARD in Mexican patients with colorectal cancer.","authors":"M. A. Rosales-Reynoso, L. Wence-Chávez, A. R. Arredondo-Valdez, S. Dumois-Petersen, Patricio Barros-Núñez, M. Gallegos-Arreola, S. Flores-Martínez, José Sánchez-Corona","doi":"10.4238/gmr16019324","DOIUrl":"https://doi.org/10.4238/gmr16019324","url":null,"abstract":"PPARD encodes for peroxisome proliferator-activated receptor delta, which plays a significant role in controlling lipid metabolism, atherosclerosis, inflammation, cancer growth, progression, and apoptosis. Accumulated evidence suggests that the polymorphism rs2016520 in PPARD is associated with lipid metabolism, obesity, metabolic syndrome, and type 2 diabetes mellitus. The aim of this study was to determine whether the single nucleotide polymorphism +294T/C (rs2016520) in PPARD is associated with colorectal cancer (CRC) in the Mexican population. Genomic DNA from 178 CRC patients and 97 healthy blood donors was analyzed. The polymorphism was identified by the polymerase chain reaction-restriction fragment length polymorphism method. Results demonstrated that patients with the T/C genotype for the +294T/C (rs2016520) polymorphism present a protective role against CRC [odds ratio (OR) = 0.39; 95% confidence interval (CI) = 0.22-0.69; P = 0.0008]. This association was also evident for the T/C genotype in the stratified analysis by tumor-node-metastasis stages I+II (OR = 0.26, P = 0.0332) and III+IV (OR = 0.44, P = 0.0067). However, in the stratified analysis by tumor location, we observed an increased risk of rectal cancer (OR = 7.57, P = 0.0403) vs colon cancer (OR = 4.87, P = 0.234) in patients carrying the C/C genotype and under the dominant and recessive models of inheritance. In conclusion, for the first time, the association between the +294T/C (rs2016520) polymorphism and colorectal cancer has been studied in Mexican patients. Our results reveal that variations in PPARD may play a significant role in genetic susceptibility to colorectal cancer.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128110977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca. 细胞周期中45S rDNA位点的动态:禾草和羊茅的脆性位点和染色体稳定性。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019156

L. Rocha, G. A. Silva, F. O. Bustamante, R. A. D. Silveira, A. Mittlemann, V. H. Techio

{"title":"Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca.","authors":"L. Rocha, G. A. Silva, F. O. Bustamante, R. A. D. Silveira, A. Mittlemann, V. H. Techio","doi":"10.4238/gmr16019156","DOIUrl":"https://doi.org/10.4238/gmr16019156","url":null,"abstract":"Analyses carried out with fluorescence in situ hybridization (FISH) in C-metaphases of the Lolium-Festuca complex have shown the occurrence of spontaneous fragile sites (FSs) in 45S rDNA regions. FSs are expressed as gaps but they do not result in breaks or chromosomal fragments in these species. These gaps have high DNA condensation observed as thin chromatin fibers that connect the apparent segments of the fragile chromosome, allowing for genomic stability. Assessing the behavior of these regions in the cell cycle of Lolium and Festuca species may lead to a better understanding of the dynamics that preserve stability during cell division. Furthermore, it is interesting to track the dynamics of chromosomes bearing 45S rDNA sites in the cell cycle as well as to observe the expression of FSs with no effect of the mitotic block. We observed variation in both the number and size of 45S FISH signals from the S/G2 phases of interphase and from prophase to anaphase where gaps in 45S rDNA sites also were observed. The change in the degree of condensation of the 45S site begins in the S/G2 phase and appears to be related to the transcriptional demand. Taking into account that the number of 45S rDNA sites tends to be re-established when cells reach telophase, we suggest that the chromatin fiber goes back to the normal condensation level to the anaphase (after segregation), allowing for the approximation of chromosome segments and ensuring dynamics that favor the genomic stability of these species.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129914768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Transfer and expression of the rabbit defensin NP-1 gene in lettuce (Lactuca sativa). 兔防御素NP-1基因在莴苣中的转移与表达。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019333

D. Song, X. Xiong, W. Tu, W. Yao, H. Liang, F. J. Chen, Z. He

引用次数: 2

Mitochondrial DNA insertions in the nuclear Capra hircus genome. 卡普拉细胞核基因组的线粒体DNA插入。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16018266

F. Ning, J. Fu, Zhiheng Du

引用次数: 6