NematologyPub Date : 2023-04-13DOI: 10.1163/15685411-bja10243
J. Gu, Xinxin Ma, Yiwu Fang
{"title":"Devibursaphelenchus wangi (Nematoda: Ektaphelenchinae) detected in Loblolly pine logs from the USA with morphological and molecular differences","authors":"J. Gu, Xinxin Ma, Yiwu Fang","doi":"10.1163/15685411-bja10243","DOIUrl":"https://doi.org/10.1163/15685411-bja10243","url":null,"abstract":"","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47102366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NematologyPub Date : 2023-04-13DOI: 10.1163/15685411-bja10237
Clemen J. Oliveira, E. van Santen, M. Marin, L. Schumacher, N. Peres, J. Desaeger
{"title":"Susceptibility of seven strawberry cultivars to Belonolaimus longicaudatus and interaction with Phytophthora cactorum","authors":"Clemen J. Oliveira, E. van Santen, M. Marin, L. Schumacher, N. Peres, J. Desaeger","doi":"10.1163/15685411-bja10237","DOIUrl":"https://doi.org/10.1163/15685411-bja10237","url":null,"abstract":"\u0000Belonolaimus longicaudatus and Phytophthora cactorum are pathogens threatening Florida strawberry production. The interaction among these and cultivar susceptibility were assessed in this study. Cultivars ‘Sensation® Florida127’, ‘Florida Brilliance’, ‘Florida Radiance’, ‘Winterstar™ FL 05-107’, ‘Florida Elyana’, ‘Strawberry Festival’ and ‘Florida Beauty’ were used to conduct one trial using plug transplants and a second trial using bare-root transplants, which was repeated twice. A factorial combination of P. cactorum (+/−) and B. longicaudatus (+/−) inoculation was applied to evaluate strawberry cultivar resistance to P. cactorum and B. longicaudatus as well as their interaction. The effect of P. cactorum on the canopy, root length, and root and shoot weight was dependent on cultivars. For the plug transplant trial, the overall average of plant mortality among all tested strawberry cultivars was 0.1%, suggesting higher tolerance against P. cactorum when vigorous transplants were used. Conversely, the repeated bare-root transplant trial demonstrated different levels of susceptibility in strawberry cultivars when transplants were inoculated with P. cactorum. ‘Sweet Sensation’ ‘Florida127’, ‘Florida Brilliance’, ‘Florida Radiance’ and ‘Winterstar FL 05-107’ were highly susceptible, whereas ‘Florida Elyana’ and ‘Strawberry Festival’ were resistant. Moreover, ‘Florida Beauty’ was moderately resistant to P. cactorum. Our findings demonstrated the importance of using vigorous transplants to reduce P. cactorum damage. Sting nematode reproduction was suppressed when strawberry transplants were inoculated with P. cactorum, suggesting an antagonistic interaction between the pathogens. None of the cultivars was tolerant to B. longicaudatus as this nematode reproduced from 13.4- to 23.8-fold and caused root damage under glasshouse conditions.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43482348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NematologyPub Date : 2023-04-12DOI: 10.1163/15685411-bja10241
Dang-Minh-Chanh Nguyen, Thi-Hoan Luong, X. Nguyen, W. Jung
{"title":"Nematicidal and antioxidant activities of the methanolic extract from Tithonia diversifolia grown in Vietnam","authors":"Dang-Minh-Chanh Nguyen, Thi-Hoan Luong, X. Nguyen, W. Jung","doi":"10.1163/15685411-bja10241","DOIUrl":"https://doi.org/10.1163/15685411-bja10241","url":null,"abstract":"\u0000This study aimed to evaluate the nematicidal activity of the Tithonia diversifolia leaf extract (TDE) against Meloidogyne incognita under in vitro and pot conditions. In addition, the antioxidant activity of TDE was evaluated using the 2,2-Diphenyl-2-picrylhydrazyl hydrate (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The results revealed that TDE possesses antioxidant potential. Nematicidal activity bioassays revealed that the TDE treatment resulted in 3.0, 16.3, 36.6, 52.4 and 64.2% second-stage juvenile (J2) hatch inhibition at MeOH control, 0.125, 0.25, 0.5 and 1.0 mg ml−1, respectively, for a 5-day treatment. The corresponding values for a 10-day treatment were 3.3, 16.0, 30.7, 56.0 and 69.3%, respectively. The TDE treatment resulted in 64.6, 86.4, 95.3 and 100% J2 movement inhibition at 0.125, 0.25, 0.5 and 1.0 mg ml−1, respectively, for a 48 h treatment. In pot experiments, at 60 days after treatment (DAT), the J2 populations in non-treated, 0.25, 0.5, 1.0 mg TDE (g soil)−1, and Tervigo 020SC 1.0 mg (g soil)−1 were 477.1, 277.3, 142.7, 23.9 and 19.7 per 5 g of root, respectively. The corresponding values in 100 g of soil were 33.2, 5.5, 3.6, 1.1 and 4.0, respectively. The relative galling formation on the Robusta coffee plants was 100, 55.3, 39.1, 33.5 and 80.8% in the non-treated, 0.25, 0.5, 1.0 mg TDE (g soil)−1, and Tervigo-treated plants, respectively. These results may provide crucial information for exploring the nematicidal metabolites of TDE against M. incognita. Therefore, the purified active compounds of TDE should be identified in future studies before the commercialisation of TDE for the biocontrol of M. incognita.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45547809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Description of Laimaphelenchus shanxiensis sp. n. (Rhabditida: Aphelenchoididae) from Shanxi province, P.R. China","authors":"Liyi Wang, Tian Yin, Yimeng Yang, Z. Zhao, Jianming Wang, Yumei Xu","doi":"10.1163/15685411-bja10239","DOIUrl":"https://doi.org/10.1163/15685411-bja10239","url":null,"abstract":"\u0000Laimaphelenchus shanxiensis sp. n. was recovered from the bark samples of Chinese pine tree (Pinus tabuliformis) in Shanxi province, China. The new species is characterised by having 888-1049 μm long females, the cephalic region with six labial sectors divided by ribs, stylet 11-13 μm long, the excretory pore located at the same level with, or slightly posterior to nerve ring, lateral field with four incisures, post-vulval uterine sac 54-83 μm long, vulva with a well-developed anterior flap, tail having a single stalk at terminus ending to a flat structure with 4-7 finger-like appendages in scanning electron microscopy; male spicules 20-22 μm long and two pairs of caudal papillae present. In addition, molecular phylogenetic trees inferred from the partial small subunit (SSU) and D2-D3 segments of large subunit (LSU) of rRNA genes, revealed that this nematode belongs to the Laimaphelenchus and is different from all currently sequenced species of Laimaphelenchus for their SSU and LSU markers.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49628953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NematologyPub Date : 2023-04-05DOI: 10.1163/15685411-bja10242
M. Asgari, Mahsa Mohammadlou, A. Eskandari, J. Abolafia, R. Peña‐Santiago
{"title":"Morphological and molecular characterisation of Talanema maculatum sp. n. (Dorylaimida, Qudsianematidae) from Iran","authors":"M. Asgari, Mahsa Mohammadlou, A. Eskandari, J. Abolafia, R. Peña‐Santiago","doi":"10.1163/15685411-bja10242","DOIUrl":"https://doi.org/10.1163/15685411-bja10242","url":null,"abstract":"\u0000A new species of the genus Talanema, T. maculatum sp. n., collected in a natural enclave of Iran, is characterised on the basis of morphological and molecular data. It is distinguishable by its 1.82-2.20 mm long body, lip region offset by constriction and 20-23 μm wide with perioral liplets, odontostyle 21.5-28 μm long, guiding ring double, neck 394-525 μm long, pharyngeal expansion occupying 44-49% of the total neck length, uterus tripartite and 308-441 μm long or 5.1-6.8 body diam., vulva transverse (V = 54-58), presence of abundant cuticle irregularities at the perivulval area, tail similar in both sexes, conical with a dorsal concavity (31-45 μm, c = 46-71, c′ = 0.9-1.1), spicules 70-75 μm long, and 14-18 shortly spaced ventromedian supplements ending at level of the anterior end of spicules, with hiatus. Molecular analysis of 28S-rDNA gene confirms the monophyly of Talanema and the difficulties in elucidating its external evolutionary relationships.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49072711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NematologyPub Date : 2023-03-31DOI: 10.1163/15685411-bja10236
N. Kanzaki, Ryusei Tanaka, R. Giblin-Davis
{"title":"Schistonchus pumilae n. sp. isolated from syconia of a creeper fig, Ficus pumila L. from the Japanese warm temperate zone","authors":"N. Kanzaki, Ryusei Tanaka, R. Giblin-Davis","doi":"10.1163/15685411-bja10236","DOIUrl":"https://doi.org/10.1163/15685411-bja10236","url":null,"abstract":"\u0000A Schistonchus species was isolated from the syconia of the creeping fig, Ficus pumila, collected from Miyazaki, Japan. The nematode was considered an undescribed species based on its typological characters, molecular profiles (near full length of small subunit and D3 expansion segment of large subunit of RNA) and phylogenetic status inferred from these two loci. The new species is characterised by the presence of flagellate sperm, spicule possessing small condylus, triangular rostrum with bluntly pointed tip and clear dorsal and ventral limbs, structure and arrangement of male caudal papillae, i.e., presence of papilliform P3 and P3a and small and gland opening-like P4 (glandpapillae), relatively short post-uterine sac and female tail forming elongate conoid. It is phylogenetically close to S. hirtus. However, the new species is distinguished from other nominal Schistonchus species by its female tail shape forming elongate conoid, the structure and arrangement of male caudal papillae and several other typological characters. In addition, the host fig of the nematode, F. pumila, is a creeper fig species belonging to subgenus Synoecia (section Rhizocladus; subsection Plagiostigma), from which no fig-associated nematode has been reported so far, i.e., the present study is the first report of Schistonchus (and other syconia-inhabiting nematodes) from the Ficus subgenus Synoecia.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45580533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NematologyPub Date : 2023-03-31DOI: 10.1163/15685411-bja10233
N. Kanzaki, H. Kosaka, Yuta Fujimori, M. Akiba
{"title":"On the phylogenetic status of Contortylenchus genitalicola Kosaka & Ogura, 1993","authors":"N. Kanzaki, H. Kosaka, Yuta Fujimori, M. Akiba","doi":"10.1163/15685411-bja10233","DOIUrl":"https://doi.org/10.1163/15685411-bja10233","url":null,"abstract":"\u0000Contortylenchus genitalicola is a unique species within the genus. It parasitises the ovary or testis of the longhorn beetle, Monochamus alternatus, and has a neotylenchid-like culturable mycetophagous phase within its life cycle, while the other species in the genus parasitise bark beetles and have a typical unculturable allantonematid life cycle. Because the species was described based solely on morphology, it was necessary to confirm the phylogenetic and taxonomic status of C. genitalicola. A molecular phylogenetic analysis of the species based on ribosomal RNA sequences revealed that C. genitalicola forms a well-supported clade with Contortylenchus spp. isolated from bark beetles, an unidentified allantonematid from Iran, and ‘Deladenus cf. laricis’ isolated from the metallic wood-boring beetle, Agrilus macer LeConte (Coleoptera: Buprestidae), in the USA. The generic status of C. genitalicola was supported by phylogenetic analysis, and the analysis also suggests that the diversity of the genus could be higher than currently expected.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45244761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NematologyPub Date : 2023-03-31DOI: 10.1163/15685411-bja10238
Mohammad Ali Poureskandarian, E. Pourjam, M. Pedram
{"title":"New evidence on cryptic speciation in soil-inhabiting nematodes, with morphological and molecular characterisation of Xiphinema sangesarense n. sp. (Dorylaimida: Longidoridae) from north-central mountains of Iran","authors":"Mohammad Ali Poureskandarian, E. Pourjam, M. Pedram","doi":"10.1163/15685411-bja10238","DOIUrl":"https://doi.org/10.1163/15685411-bja10238","url":null,"abstract":"\u0000A new species of the dagger nematode, genus Xiphinema, was recovered from the rhizospheric soil samples collected from rangelands in Semnan province, north-central Iran, and described based upon both morphological and molecular data. Xiphinema sangesarense n. sp. is characterised by 3.7-4.8 mm long females having 136-154 μm long odontostyle, 74-87 μm long odontophore, two equally developed genital tracts with crystalloid bodies in the tubular part of the uterus, short dorsally convex, ventrally slightly convex tail with a mucron at the end, four juvenile developmental stages and males present, functional. The new species most closely resembles X. azarbaijanense and is regarded as its tentative cryptic form, but was separated from it by reproductive mode, and morphological and molecular differences. Compared to X. afratakhtehense, the second similar species, it could be separated based on morphological differences, especially characteristics of juveniles and molecular data. Furthermore, the new species has close morphology with five know species, namely: X. aequum, X. horvatovicae, X. illyricum, X. macedonicum and X. vuittenezi, in terms of morphological characteristics (mainly structure of uterus and similar female tail), and their differences are discussed. In phylogenetic analyses of the new species using several sequences of two partial large subunit (LSU) D2-D3, and internal transcribed spacer (ITS) sequences, the new species formed a clade with Xiphinema sp. in the LSU tree, and in the ITS tree it formed a clade with X. afratakhtehense. In the cytochrome c oxidase I (COI) gene tree, sequences of the new species occupied a placement inside the clade of several sequences of X. afratakhtehense.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49449827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NematologyPub Date : 2023-03-31DOI: 10.1163/15685411-bja10240
Yongsan Zeng, L. A. Dant, J. Roberts
{"title":"Ditylenchus dactylonae n. sp. (Tylenchomorpha: Anguinidae), an associate of Cynodon dactylon (L.) Pers. in the USA","authors":"Yongsan Zeng, L. A. Dant, J. Roberts","doi":"10.1163/15685411-bja10240","DOIUrl":"https://doi.org/10.1163/15685411-bja10240","url":null,"abstract":"\u0000A new species of the genus Ditylenchus was recovered from rhizosphere soil of a ‘TifEagle’ hybrid bermudagrass (Cynodon dactylon (L.) Pers. × C. transvaalensis (Burtt-Davy)) putting green located at the Pee Dee Research & Education Center, Clemson University, Florence, SC, USA. It is described herein as Ditylenchus dactylonae n. sp. and is characterised by possessing the combined characters of a medium body size (682-815 μm in females; 618-718 μm in males), six incisures at the lateral fields, a delicate stylet (7.6-8.0 μm) with rounded knobs, a bottle-shaped basal pharyngeal bulb (BPB), an excretory pore (EP) near anterior end of BPB, a posteriorly located vulva (V = 78-83%), a reflexed ovary, a uterus with crustaformeria anteriorly and nearly spherical structure posteriorly, a post-vulval uterine sac (30-50% of the vulva-anus distance), an elongate conoid and ventrally bent tail, a tylenchoid spicule (17-18 μm), and a leptoderan bursa. Ditylenchus dactylonae n. sp. was differentiated from other sequenced species by the partial small subunit (SSU) rRNA gene, D2-D3 expansion segments of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) rRNA. Phylogenetic analysis revealed that D. dactylonae n. sp. belongs to the D. dipsaci-group, and is sister to D. medicaginis and/or D. ferepolitor based on SSU 18S, LSU D2-D3 and ITS sequences.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42750322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NematologyPub Date : 2023-03-08DOI: 10.1163/15685411-bja10235
Christopher Cammies, T. Prior, R. Lawson, P. Gilarte, R. Crichton
{"title":"An exploration of nematode assemblages in the hydroponic media beds of a commercial aquaponics system","authors":"Christopher Cammies, T. Prior, R. Lawson, P. Gilarte, R. Crichton","doi":"10.1163/15685411-bja10235","DOIUrl":"https://doi.org/10.1163/15685411-bja10235","url":null,"abstract":"\u0000Nematodes perform important ecosystem services in soils and are well studied in traditional farming, but their role in newer soil-less food production systems is unstudied. We determined the nematode assemblages from substrates within the hydroponic media beds of a commercial aquaponics system. Three substrates were studied: coir, compost and lightweight expanded clay aggregate (LECA), with the nematode assemblages ascertained via traditional morphological analysis. All substrates were suitable for nematodes but abundance was lower compared with traditional compost. Compost from within the hydroponic media beds had nematode assemblages with similar maturity indices (MI) to compost external to the system, and both composts housed nematodes with a high proportion of bacterivores, a high degree of enrichment and no structure. LECA that were regularly disturbed and had little organic build up resembled compost – highly enriched and lacking structure. LECA that had been left undisturbed for longer and allowed to accumulate more organic matter had highly structured assemblages and showed less enrichment than the other substrates. Nematode assemblages within the coir showed very little enrichment but had higher MI and were more structured than compost. Problematic plant-parasitic nematodes were not found in any of the samples. We present the first glimpse into the role of nematodes in aquaponics systems and encourage future studies to explore how nematodes in these systems affect yield and plant health.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42364642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}