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Nauchnye doklady vysshei shkoly. Biologicheskie nauki最新文献

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[The isolation of highly purified bacillary ribonucleases from recombinant strains of Escherichia coli]. [从大肠杆菌重组菌株中分离高纯度细菌核糖核酸酶]。
Nauchnye doklady vysshei shkoly. Biologicheskie nauki Pub Date : 1992-01-01
N P Balaban, M R Sharipova, V I Vershinina, L V Znamenskaia, E B Chernokal'skaia, E R Romakhina, T I Volkova, S V Lopatnev
{"title":"[The isolation of highly purified bacillary ribonucleases from recombinant strains of Escherichia coli].","authors":"N P Balaban,&nbsp;M R Sharipova,&nbsp;V I Vershinina,&nbsp;L V Znamenskaia,&nbsp;E B Chernokal'skaia,&nbsp;E R Romakhina,&nbsp;T I Volkova,&nbsp;S V Lopatnev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Active and inactive highly purified extracellular alkaline ribonucleases (barnase) from recombinant Escherichia coli have been prepared according to the industrial technology of Bacillus intermedius ribonuclease purification. Electrophoretic parameters and ultraviolet spectra characterize these preparations as homogeneous proteins. Immunochemical test system of identification of inactive RNAse for its purification has been worked out.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 4","pages":"68-74"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12477084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The heat denaturation of bacterial ribonuclease studied by infrared spectroscopy]. [用红外光谱研究细菌核糖核酸酶的热变性]。
Nauchnye doklady vysshei shkoly. Biologicheskie nauki Pub Date : 1992-01-01
E A Strupishina
{"title":"[The heat denaturation of bacterial ribonuclease studied by infrared spectroscopy].","authors":"E A Strupishina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The thermal denaturation of bacterial ribonuclease in the interval of pH 2.5-7.0 has been investigated by means of infra-red spectroscopy method. The protein melting for pH 2.5 begins at the temperature 25 degrees C and is accompanied by secondary protein structure reconstruction, partially destroying native beta-structure and leading to new denatured conformation appearance of different types of beta-turns. Spectral changes for pH 3.5 and 7.0 are significantly less in the same frequency areas. At the temperature more than 50 degrees C protein aggregation takes place with inter-molecule-beta-form formation.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 2","pages":"71-7"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12477225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The microbiological purification of industrial sulfur-containing sewage]. [工业含硫污水的微生物净化]。
Nauchnye doklady vysshei shkoly. Biologicheskie nauki Pub Date : 1992-01-01
Z A Arkad'eva, E V Semënova, F B Orshanskaia
{"title":"[The microbiological purification of industrial sulfur-containing sewage].","authors":"Z A Arkad'eva,&nbsp;E V Semënova,&nbsp;F B Orshanskaia","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 7","pages":"133-6"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12477648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The stimulation of the delayed hypersensitivity reaction by the venom of the blunt-nosed viper Vipera lebetina and by its liposomal form]. [钝鼻毒蛇(Vipera lebetina)的毒液及其脂质体形式对延迟超敏反应的刺激]。
Nauchnye doklady vysshei shkoly. Biologicheskie nauki Pub Date : 1992-01-01
E B Romanova, D B Gelashvili, O T Fedorov
{"title":"[The stimulation of the delayed hypersensitivity reaction by the venom of the blunt-nosed viper Vipera lebetina and by its liposomal form].","authors":"E B Romanova,&nbsp;D B Gelashvili,&nbsp;O T Fedorov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Native poison of middleasia viper Vipera lebetina has been included into liposomes in order to get an effective preparation for immunotherapy. The preparation reduces the risk of complications taking place during the use of traditional methods. The immunomodulating action of the liposomal form of viper poison has been investigated on the model of reaction of hypersensitivity of delayed type. Together with the stimulation of functional activity T-cells, the absence of suppressive and toxic actions while using the liposomal preparation of poison has been discovered. It is suggested that the observed stimulating effect in connection with immune reactions is due to synergism of action of components of poison liposomal form and activation of T-cells and macrophage, taking part in immune response to the antigenic stimulus.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 7","pages":"81-6"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12477649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of the acute and chronic administration of the opiate receptor antagonist naloxone on the development of fatigue during physical loading in rats]. [急性和慢性给药阿片受体拮抗剂纳洛酮对大鼠体力负荷疲劳发展的影响]。
Nauchnye doklady vysshei shkoly. Biologicheskie nauki Pub Date : 1992-01-01
N A Sokolova, L D Kuligina, I P Ashmarin
{"title":"[The effect of the acute and chronic administration of the opiate receptor antagonist naloxone on the development of fatigue during physical loading in rats].","authors":"N A Sokolova,&nbsp;L D Kuligina,&nbsp;I P Ashmarin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In chronic experiments with rats during the exercises on a treadmill the ECG was registered and duration of the development of exhaustion was determined under control and under acute (1 mg/kg intraperitoneally 1 hour before the experiment) or chronic (1 mg/kg intraperitoneally, twice a day during 5 days) treatment with the opioid receptor antagonist naloxone. Chronic but not acute naloxone action resulted in increase of the fatiguability: the time of achievement of exhaustion decreased by 55.3% (P < 0.05). In this case the exhaustion developed at lower degree of heart rate than in control. Comparison of data obtained with the results of chronic treatment with the opioid antagonist permits to conclude that the chronic blockade increases the fatiguability to a great extent than chronic activation of opioid system. Possible mechanisms causing this difference are under discussion.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 5","pages":"134-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12511762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of polymeric modification on the biological activity of pancreatic ribonuclease]. 聚合修饰对胰腺核糖核酸酶生物活性的影响。
Nauchnye doklady vysshei shkoly. Biologicheskie nauki Pub Date : 1992-01-01
N V Kalacheva, E G Kupriianov-Ashin, B M Kurpinenko
{"title":"[The effect of polymeric modification on the biological activity of pancreatic ribonuclease].","authors":"N V Kalacheva,&nbsp;E G Kupriianov-Ashin,&nbsp;B M Kurpinenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of modification of dextran on pharmacokinetic properties of pancreatic RNAse and on its ability to suppress the proliferation of cells has been studied. It has been shown that the basic contribution to biological activity of polymer form of RNAse is making by azo-bonds which are forming in the process of chemical bonding of the protein with dextran support.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 2","pages":"97-102"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12458576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Extracellular ribonuclease from Bacillus pumilus]. [细粒芽孢杆菌胞外核糖核酸酶]。
Nauchnye doklady vysshei shkoly. Biologicheskie nauki Pub Date : 1992-01-01
N K Struminskaia, V L Ivaĭlovskiĭ, A A Dement'ev, G P Moiseev, Iu A Fedosov, G I Iakovlev
{"title":"[Extracellular ribonuclease from Bacillus pumilus].","authors":"N K Struminskaia,&nbsp;V L Ivaĭlovskiĭ,&nbsp;A A Dement'ev,&nbsp;G P Moiseev,&nbsp;Iu A Fedosov,&nbsp;G I Iakovlev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The extracellular ribonuclease (RNAse Bp) was isolated from the cultural medium filtrate of Bacillus pumilus by ammonium sulfate precipitation and two stages of ion-exchange chromatography on carboxymethyl- and phospho-cellulose columns. The amino acid composition and N-terminal amino acid residue have been determined. The kinetic parameters of cleavage reaction of synthetic polynucleotides have been measured. According to their structural homology RNAse Bp has been shown to be similar to RNAses Ba and Bi. Catalytic properties of the enzyme are very close to RNAse Bi.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 2","pages":"41-4"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12477221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The role of the hemopoiesis-inducing microenvironment in the mechanisms of the regeneration of hemopoiesis after cytostatic exposure]. [造血诱导微环境在细胞抑制剂暴露后造血再生机制中的作用]。
Nauchnye doklady vysshei shkoly. Biologicheskie nauki Pub Date : 1992-01-01
A M Dygaĭ, V V Zhadanov, I V Bogdashin, V E Gol'dberg
{"title":"[The role of the hemopoiesis-inducing microenvironment in the mechanisms of the regeneration of hemopoiesis after cytostatic exposure].","authors":"A M Dygaĭ,&nbsp;V V Zhadanov,&nbsp;I V Bogdashin,&nbsp;V E Gol'dberg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>On the model of adriamycine-induced hemopoiesis hypoplasia it has been shown that the accelerated differentiation of hemopoietic precursors regeneration is of great importance in regeneration of hemopoiesis. This differentiation is provided by preceded recovery of bone marrow structural-functional organization. The important role in regeneration of hemopoiesis belongs to hemopoietic microenvironmental elements which express its induced influence by the increase of colony-stimulating and erythropoietic activities, interleukin-1 and interleukin-3 production.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 9","pages":"109-16"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12477754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The clinico-biological aspects of transsexualism]. [变性欲症的临床生物学方面]。
Nauchnye doklady vysshei shkoly. Biologicheskie nauki Pub Date : 1992-01-01
L V Bets, A O Bukhanovskiĭ, I V Golubeva, A S Andreev, L M Baburin, N K Shul'gina
{"title":"[The clinico-biological aspects of transsexualism].","authors":"L V Bets,&nbsp;A O Bukhanovskiĭ,&nbsp;I V Golubeva,&nbsp;A S Andreev,&nbsp;L M Baburin,&nbsp;N K Shul'gina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A complex constitutional-endocrinological investigation of persons of both sexes with clinical diagnosis of transsexualism (TS) has been held. On the basis of original data some peculiarities of somato- and cerebrotypes of transsexuals have been revealed, the formation of these peculiarities is significantly connected with the action of sexual hormones. A disturbance in ratio of male and female sexual hormones in the direction of predominance of the hormones corresponding to the patient's sexual autoidentification despite their biological sex has been marked. It gives possibility to consider that TS pathogenesis is undoubtedly connected with inborn pathology of hormonal homeostasis. The results of multi-disciplinary approach to study of TS are being discussed. An assumption about the role of disturbance in the system of \"hormones-brain\" in the TS origin is given.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 9","pages":"54-65"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12477756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[An evaluation of the genotoxicity of nonionogenic surface-active substances]. [非离子源性表面活性物质的遗传毒性评价]。
Nauchnye doklady vysshei shkoly. Biologicheskie nauki Pub Date : 1992-01-01
O B Ivanchenko, O N Il'inskaia, A N Fattakhova, I M Skipina, I E Cherepneva
{"title":"[An evaluation of the genotoxicity of nonionogenic surface-active substances].","authors":"O B Ivanchenko,&nbsp;O N Il'inskaia,&nbsp;A N Fattakhova,&nbsp;I M Skipina,&nbsp;I E Cherepneva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mutagenic activity of 16 industrial nonionic surfactant samples has been investigated in Ames-test without metabolic activation and with the use of mouse liver microsomal fraction in vitro. The genotoxical estimation of their possible biodegradation products is given. The mutagenicity of a number of high molecular polyethylene glycoles related to nonionic surfactants is shown.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 1","pages":"75-80"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12474203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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