Molecular Membrane Biology最新文献_第8页

Significance of the anti-aging protein Klotho. 抗衰老蛋白Klotho的意义。

Molecular Membrane Biology Pub Date : 2013-12-01 Epub Date: 2013-10-14 DOI: 10.3109/09687688.2013.837518

Miribane Dërmaku-Sopjani, Selim Kolgeci, Sokol Abazi, Mentor Sopjani

引用次数: 45

Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties. 人成骨细胞表面存在偏硅酸钠受体的证据空间定位和绑定属性。

Molecular Membrane Biology Pub Date : 2013-12-01 Epub Date: 2013-10-23 DOI: 10.3109/09687688.2013.843031

Kelly-Ann Vere, Joanna L Richens, Jordan S Lane, Helen J Harris, James Duggan, Paul O'Shea

引用次数: 0

Location and membrane sources for autophagosome formation - from ER-mitochondria contact sites to Golgi-endosome-derived carriers. 自噬体形成的位置和膜源——从er -线粒体接触位点到高尔基核内体衍生载体。

Molecular Membrane Biology Pub Date : 2013-12-01 Epub Date: 2013-11-01 DOI: 10.3109/09687688.2013.850178

Shu Ning Chan, Bor Luen Tang

{"title":"Location and membrane sources for autophagosome formation - from ER-mitochondria contact sites to Golgi-endosome-derived carriers.","authors":"Shu Ning Chan, Bor Luen Tang","doi":"10.3109/09687688.2013.850178","DOIUrl":"https://doi.org/10.3109/09687688.2013.850178","url":null,"abstract":"Recent advances have revealed much about the signaling events and molecular components associated with autophagy. Although it is clear that there are multiple points of intersection and connection between autophagy and known vesicular membrane transport processes between membrane compartments, autophagy is modulated by a distinct set of molecular components, and the autophagosome has a unique membrane composition. A key question that has yet to be resolved with regards to autophagosome formation is its membrane source. Various evidences have indicated that membranes from the endoplasmic reticulum (ER), mitochondria, Golgi, endosomes and the plasma membrane could all potentially act as a source of autophagosomal membrane in non-specialized macroautophagy. Recent investigations have generated advances in terms of the mitochondria's involvement in starvation-induced autophagy, and refined localization of autophagosome formation to ER-mitochondria contact sites. On the other hand, data accumulates on the delivery of membrane sources to the pre-autophagosome structure by Atg9-containing mobile carriers, which likely originated from the Golgi-endosome system. The answer to the question on the origin of membrane materials for autophagosome biogenesis awaits further reconciliation of these different findings.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":" ","pages":"394-402"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2013.850178","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40280124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42

Impact of histidine residues on the transmembrane helices of viroporins. 组氨酸残基对病毒孔蛋白跨膜螺旋的影响。

Molecular Membrane Biology Pub Date : 2013-11-01 Epub Date: 2013-10-09 DOI: 10.3109/09687688.2013.842657

Yan Wang, Sang Ho Park, Ye Tian, Stanley J Opella

{"title":"Impact of histidine residues on the transmembrane helices of viroporins.","authors":"Yan Wang, Sang Ho Park, Ye Tian, Stanley J Opella","doi":"10.3109/09687688.2013.842657","DOIUrl":"https://doi.org/10.3109/09687688.2013.842657","url":null,"abstract":"Abstract The role of histidine in channel-forming transmembrane (TM) helices was investigated by comparing the TM helices from Virus protein 'u' (Vpu) and the M2 proton channel. Both proteins are members of the viroporin family of small membrane proteins that exhibit ion channel activity, and have a single TM helix that is capable of forming oligomers. The TM helices from both proteins have a conserved tryptophan towards the C-terminus. Previously, alanine 18 of Vpu was mutated to histidine in order to artificially introduce the same HXXXW motif that is central to the proton channel activity of M2. Interestingly, the mutated Vpu TM resulted in an increase in helix tilt angle of 11° in lipid bilayers compared to the wild-type Vpu TM. Here, we find the reverse, when histidine 37 of the HXXXW motif in M2 was mutated to alanine, it decreased the helix tilt by 10° from that of wild-type M2. The tilt change is independent of both the helix length and the presence of tryptophan. In addition, compared to wild-type M2, the H37A mutant displayed lowered sensitivity to proton concentration. We also found that the solvent accessibility of histidine-containing M2 is greater than without histidine. This suggests that the TM helix may increase the solvent exposure by changing its tilt angle in order to accommodate a polar/charged residue within the hydrophobic membrane region. The comparative results of M2, Vpu and their mutants demonstrated the significance of histidine in a transmembrane helix and the remarkable plasticity of the function and structure of ion channels stemming from changes at a single amino acid site. ","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"30 7","pages":"360-9"},"PeriodicalIF":0.0,"publicationDate":"2013-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2013.842657","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31787242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Lipase activity in lipidomics - a hidden problem? 脂质组学中的脂肪酶活性——一个隐藏的问题?

Molecular Membrane Biology Pub Date : 2013-11-01 Epub Date: 2013-09-09 DOI: 10.3109/09687688.2013.835125

Samuel Furse, J Antoinette Killian

引用次数: 4

Getting into the cilia: nature of the barrier(s). 进入纤毛:屏障的性质。

Molecular Membrane Biology Pub Date : 2013-11-01 Epub Date: 2013-10-08 DOI: 10.3109/09687688.2013.842003

Yi Shan Lim, Bor Luen Tang

引用次数: 7

How do transporters couple solute movements? 转运体是如何结合溶质运动的?

Molecular Membrane Biology Pub Date : 2013-11-01 DOI: 10.3109/09687688.2013.842658

Gary Rudnick

引用次数: 16

Efficiency of the monofunctionalized C60 fullerenes as membrane targeting agents studied by all-atom molecular dynamics simulations. 用全原子分子动力学模拟研究了单官能化C60富勒烯作为膜靶向剂的效率。

Molecular Membrane Biology Pub Date : 2013-08-01 DOI: 10.3109/09687688.2013.828856

Semen O Yesylevskyy, Sebastian Kraszewski, Fabien Picaud, Christophe Ramseyer

引用次数: 5

Expression, characterization and ligand specificity of lipocalin-1 interacting membrane receptor (LIMR). 脂钙素-1相互作用膜受体(LIMR)的表达、表征及配体特异性。

Molecular Membrane Biology Pub Date : 2013-08-01 DOI: 10.3109/09687688.2013.823018

Renske W Hesselink, John B C Findlay

{"title":"Expression, characterization and ligand specificity of lipocalin-1 interacting membrane receptor (LIMR).","authors":"Renske W Hesselink, John B C Findlay","doi":"10.3109/09687688.2013.823018","DOIUrl":"https://doi.org/10.3109/09687688.2013.823018","url":null,"abstract":"Human lipocalin-1 interacting membrane receptor (LIMR) was the first lipocalin receptor to be identified, as a specific receptor for lipocalin-1 (Lcn1). Subsequently LIMR has been reported to interact with other ligands as well, notably with the bovine lipocalin β-lactoglobulin (BLG) and with the unrelated secretoglobin uteroglobin (UG). To study the ligand-binding behaviour of this prototypic lipocalin receptor in more detail, a system was developed for the recombinant expression of LIMR in Drosophila Schneider 2 (S2) cells, and for the subsequent solubilization and purification of the protein. The receptor forms dimers or larger oligomers when solubilized in n-dodecyl β-D-maltoside (DDM). The full-length, functional receptor was captured onto a surface plasmon resonance (SPR) chip via an α-V5 antibody, and the binding of various potential ligands was followed in time. In this way, LIMR was shown to be highly specific for Lcn1, binding the lipocalin with low micromolar to high nanomolar affinity. No interactions with any of the other putative ligands could be detected, raising doubts about the physiological relevance of the reported binding of BLG and UG to the receptor.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"30 5-6","pages":"327-37"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2013.823018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31673487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Lysophosphatidylcholine-induced cytotoxicity in osteoblast-like MG-63 cells: involvement of transient receptor potential vanilloid 2 (TRPV2) channels. 溶血磷脂酰胆碱诱导成骨细胞样MG-63细胞的细胞毒性:瞬时受体电位香兰素2 (TRPV2)通道的参与

Molecular Membrane Biology Pub Date : 2013-08-01 DOI: 10.3109/09687688.2013.828855

Abdallah Fallah, Rachel Pierre, Elie Abed, Robert Moreau

{"title":"Lysophosphatidylcholine-induced cytotoxicity in osteoblast-like MG-63 cells: involvement of transient receptor potential vanilloid 2 (TRPV2) channels.","authors":"Abdallah Fallah, Rachel Pierre, Elie Abed, Robert Moreau","doi":"10.3109/09687688.2013.828855","DOIUrl":"https://doi.org/10.3109/09687688.2013.828855","url":null,"abstract":"Epidemiological studies indicate that patients suffering from atherosclerosis are predisposed to develop osteoporosis. Accordingly, atherogenic determinants such as oxidized low density lipoprotein (OxLDL) particles have been shown to alter bone cell functions. In this work, we investigated the cytotoxicity of lysophosphatidylcholine (lysoPC), a major phospholipid component generated upon LDL oxidation, on bone-forming MG-63 osteoblast-like cells. Cell viability was reduced by lysoPC in a concentration-dependent manner with a LC50 of 18.7±0.7 μM. LysoPC-induced cell death was attributed to induction of both apoptosis and necrosis. Since impairment of intracellular calcium homeostasis is often involved in mechanism of cell death, we determined the involvement of calcium in lysoPC-induced cytotoxicity. LysoPC promoted a rapid and transient increase in intracellular calcium attributed to mobilization from calcium stores, followed by a sustained influx. Intracellular calcium mobilization was associated to phospholipase C (PLC)-dependent mobilization of calcium from the endoplasmic reticulum since inhibition of PLC or calcium depletion of reticulum endoplasmic with thapsigargin prevented the calcium mobilization. The calcium influx induced by lysoPC was abolished by inhibition of transient receptor potential vanilloid (TRPV) channels with ruthenium red whereas gadolinium, which inhibits canonical TRP (TRPC) channels, was without effect. Accordingly, expression of TRPV2 and TRPV4 were shown in MG-63 cells. The addition of TRPV2 inhibitor Tranilast in the incubation medium prevent the calcium influx triggered by lysoPC and reduced lysoPC-induced cytotoxicity whereas TRPV4 inhibitor RN 1734 was without effect, which confirms the involvement of TRPV2 activation in lysoPC-induced cell death.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"30 5-6","pages":"315-26"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2013.828855","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31673488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12