Molecular biotherapy最新文献_第8页

Distribution of human recombinant interferon-alpha 2 in rat plasma, liver, and experimental liver metastases. 人重组干扰素- α 2在大鼠血浆、肝脏和实验性肝转移中的分布。

Molecular biotherapy Pub Date : 1990-12-01

V Bocci, F Carraro, A Naldini, P P Cagol, E M Pasqual, C Prevaldi, D Casara

引用次数: 0

Treatment of rotavirus infection in neonate and weanling pigs using natural human interferon alpha. 天然人α干扰素治疗新生儿和断奶仔猪轮状病毒感染。

Molecular biotherapy Pub Date : 1990-12-01

J G Lecce, J M Cummins, A B Richards

{"title":"Treatment of rotavirus infection in neonate and weanling pigs using natural human interferon alpha.","authors":"J G Lecce, J M Cummins, A B Richards","doi":"","DOIUrl":"","url":null,"abstract":"Colostrum-deprived neonate piglets challenged with rotavirus and 3-week-old newly weaned piglets naturally exposed to rotavirus were treated with low doses of natural human interferon alpha (nHuIFN alpha) administered into the oral cavity or included in the liquid diet. The colostrum-deprived piglets given the highest dosage of nHuIFN alpha (50 IU/kg body weight) had lower viral excretion scores at 3 (p less than 0.11) and 4 days (p less than 0.001) after virus inoculation. Average group weights and weight gains were consistently greater for all nHuIFN alpha-treated neonate groups; however, these differences were not significant. Mortality rates were lower in neonates for the three highest nHuIFN alpha treatment groups (20%, 30%, and 20%) than in the lowest treatment group and controls (60% and 45%, respectively). Three-week-old weanling piglets did not have significant differences in the total average diarrhea or rotavirus excretion scores. After 10 days, the group receiving the highest dosage of nHuIFN alpha had significantly greater average weight gain than the control group (p less than 0.05). There was a significant (p less than 0.01) dose-dependent effect between the logarithm10 of the nHuIFN alpha dosage and weight gain in weanling piglets. There were no death losses in the 3-week-old weanling piglets from natural rotavirus exposure.","PeriodicalId":18809,"journal":{"name":"Molecular biotherapy","volume":"2 4","pages":"211-6"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13121191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential therapeutic applications of PHA-L4, the mitogenic isolectin of phytohemagglutinin. 植物血凝素有丝分裂分离素PHA-L4的潜在治疗应用。

Molecular biotherapy Pub Date : 1990-12-01

B M Wimer

引用次数: 0

Prostaglandin E2-mediated suppression of murine lymphokine-activated killer cell activity generated from tumor-bearing hosts by interferon-gamma. 前列腺素e2介导的干扰素抑制荷瘤宿主产生的小鼠淋巴因子激活杀伤细胞活性。

Molecular biotherapy Pub Date : 1990-12-01

I Nakajima, T M Chu

{"title":"Prostaglandin E2-mediated suppression of murine lymphokine-activated killer cell activity generated from tumor-bearing hosts by interferon-gamma.","authors":"I Nakajima, T M Chu","doi":"","DOIUrl":"","url":null,"abstract":"The effect of mouse recombinant interferon-gamma (IFN-gamma) on murine lymphokine-activated killer (LAK) cell activity was investigated using a natural killer-resistant, LAK-sensitive, spontaneously developed, weakly immunogenic, syngeneic murine mammary adenocarcinoma, a tumor model mimicking that of human disease. When all of the splenocytes prepared from tumor-bearing mice were cultured with recombinant interleukin-2 (IL-2) and IFN-gamma, LAK cell activity was suppressed in an IFN-gamma dose-dependent manner. An increase in the prostaglandin E2 (PGE2) content in the corresponding culture media was detected, as was IFN-gamma dose dependent. The suppression of generation of LAK cell activity by IFN-gamma was abrogated, accompanied by the elimination of the increase in PGE2 content, when plastic dish and nylon wool-treated nonadherent macrophage-depleted splenocytes were used. These results indicated that IL-2-induced LAK cell activity generated from the splenocytes of tumor-bearing mice was suppressed by IFN-gamma, and that PGE2 secreted from the macrophages of the splenocyte cultures served as the mediator in this IFN-gamma dose-dependent suppression of IL-2-induced LAK cell activity.","PeriodicalId":18809,"journal":{"name":"Molecular biotherapy","volume":"2 4","pages":"228-32"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13282213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential treatment of melanoma patients who progressed on interleukin-2 and dacarbazine by alpha-interferon and dacarbazine--a preliminary report. 初步报告-干扰素和达卡巴嗪序次治疗进展的白介素-2和达卡巴嗪黑色素瘤患者

Molecular biotherapy Pub Date : 1990-12-01

O Merimsky, M Inbar, E Shiloni, I Ron, S Chaitchik

引用次数: 0

Chronic major aphthous stomatitis: oral treatment with low-dose alpha-interferon. 慢性重度口腔炎:低剂量干扰素口服治疗。

Molecular biotherapy Pub Date : 1990-12-01

V A Hutchinson, W L Mok, J L Angenend, J M Cummins, A B Richards

引用次数: 0

Detection of cellular platinum using the monoclonal antibody 1C1. 单克隆抗体1C1检测细胞铂。

Molecular biotherapy Pub Date : 1990-12-01

K Nishikawa, R A Newman, L Murray, A R Khokhar, M G Rosenblum

{"title":"Detection of cellular platinum using the monoclonal antibody 1C1.","authors":"K Nishikawa, R A Newman, L Murray, A R Khokhar, M G Rosenblum","doi":"","DOIUrl":"","url":null,"abstract":"A monoclonal antibody, 1C1, developed using a trans-R,R-1,2-diamminocyclohexane (DACH) modified platinum analog (DACH-Pt-SO4) complexed with DNA was shown, using the enzyme-linked immunosorbent assay (ELISA), to have the ability to bind to both free drug DACH-Pt-SO4 and to the drug-DNA complex. Using competitive ELISA, 1C1 was found to recognize non-DACH-containing platinum compounds, such as cis-dichlorodiammine platinum (II) (CDDP). 1C1 exhibited strong binding to slot-blotted, DACH-Pt-SO4-treated DNA and moderate binding to the CDDP-DNA complex, but was unable to detect DACH containing methyliminodiacetato-trans-R,R-1,2-diamminocyclohexane platinum (II) (MIDP)-modified DNA. Immunocytochemical staining studies using 1C1 antibody on CDDP-treated BRO melanoma cells showed preferential staining of the cytosol compared with the nucleus. Although the extent of staining was dose dependent, a heterogeneous staining pattern was observed. Multicellular spheroids of MDA886LN squamous carcinoma cells treated with CDDP showed intense staining on the growing periphery compared with weak but homogeneous staining within the spheroid. Cell cycle-dependent uptake of CDDP in synchronized BRO cells may partly explain the observed heterogeneity of platinum distribution.","PeriodicalId":18809,"journal":{"name":"Molecular biotherapy","volume":"2 4","pages":"235-41"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13439124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Set my factors free. 让我的因素自由。

Molecular biotherapy Pub Date : 1990-12-01

R K Oldham

引用次数: 0

Modification of lymphokine-activated killer cell accumulation into tumor sites by chemotherapy, local irradiation, or splenectomy. 化疗、局部照射或脾切除术对淋巴因子激活的杀伤细胞在肿瘤部位积聚的影响。

Molecular biotherapy Pub Date : 1990-12-01

A Kawata, M Hosokawa, Y Sawamura, K Ito, Y Une, T Shibata, J Uchino, H Kobayashi

{"title":"Modification of lymphokine-activated killer cell accumulation into tumor sites by chemotherapy, local irradiation, or splenectomy.","authors":"A Kawata, M Hosokawa, Y Sawamura, K Ito, Y Une, T Shibata, J Uchino, H Kobayashi","doi":"","DOIUrl":"","url":null,"abstract":"The effects of chemotherapy or local irradiation on lymphokine-activated killer (LAK) cell accumulation into tumor sites were investigated. Lymphokine-activated killer cells labeled with 111In-oxine were injected into the caudal vein of C57BL/6 mice that had been previously transplanted with 3LL cancer. An adoptive transfer of LAK cells was carried out 4 days after treatments. Twenty-four hours after the transfer, tumor tissues were excised, and the accumulation of labeled LAK cells in the tumor was measured. In two different experiments, LAK cell accumulation in tumor in the nontreated group was 2.15% and 1.58% of the administered dose per gram of tissue. The accumulation in the groups of mice treated with cyclophosphamide, nimustine hydrochloride, or Adriamycin increased fourfold (7.38% dose/g, 6.61% dose/g), threefold (6.47% dose/g) and twofold (4.46% dose/g), respectively, as compared with the nontreated group. These agents induced significant tumor regression. In the group treated with bleomycin, which showed no significant effect on tumor growth, LAK cell accumulation in tumor remained unaltered (1.57% dose/g). However, the group treated with local irradiation, which induced significant tumor reduction, showed no increase in LAK cell accumulation into tumors. These results suggest that some antitumor drugs enhance LAK cell accumulation into tumor sites and that this increase is due to tumor modification by antitumor drugs.","PeriodicalId":18809,"journal":{"name":"Molecular biotherapy","volume":"2 4","pages":"221-7"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13438539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The phylogeny of oncology. 肿瘤学的系统发育。

Molecular biotherapy Pub Date : 1990-09-01

M R Lentz

引用次数: 0