Microcirculation, endothelium, and lymphatics最新文献_第7页

Immunocytochemical localization of glyceraldehyde-3-phosphate dehydrogenase in cultured endothelial and smooth muscle cells. 内皮细胞和平滑肌细胞中甘油醛-3-磷酸脱氢酶的免疫细胞化学定位。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-12-01

P Hodor, C Heltianu

引用次数: 0

Concentration dependent effects of calcium blockade on vessel diameter and microvascular macromolecular clearance. 钙阻断剂对血管直径和微血管大分子清除的浓度依赖性影响。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-12-01

J Paul, W N Durán

引用次数: 0

Effect of carbon dioxide on the utilization of brain capillary reserve and flow. 二氧化碳对脑毛细血管储备和流量利用的影响。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-12-01

H Frankel, J Dribben, I Kissen, T Gerlock, H R Weiss

{"title":"Effect of carbon dioxide on the utilization of brain capillary reserve and flow.","authors":"H Frankel, J Dribben, I Kissen, T Gerlock, H R Weiss","doi":"","DOIUrl":"","url":null,"abstract":"This study investigated effects of increased arterial carbon dioxide on the brain capillary perfusion pattern. Conscious rats were exposed to a 0%, 8% or 12% CO2 in air gas mixture. Arterial blood pressure, heart rate, arterial blood gases and pH were recorded, and either regional cerebral blood flow or the percent of capillary volume/mm3 or number/mm2 perfused were determined in cortical, hypothalamic, pontine or medullary regions of the brain. Arterial PCO2 increased from 37 +/- 1 in control to 74 +/- 1 torr in the high CO2 group. A position linear relationship was found between cerebral blood flow and arterial PCO2 in all examined regions. Approximately half of the capillaries in the examined regions were perfused under normocapnic conditions. Increasing arterial PCO2 had no effect on the percent of the capillary bed perfused in the cortex or hypothalamus. However, there was a significant linear relationship between carbon dioxide tension and the percent of the microvasculature perfused in the hindbrain. The percent of capillaries/mm2 perfused increased significantly in the medulla (to 60 +/- 5%) and pons (70 +/- 4%) with 12% CO2 in air. These data suggest that carbon dioxide may have differential effects on diffusion distances affecting the hindbrain to a greater extent than the forebrain.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"5 6","pages":"391-415"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13661517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flow velocity-dependent regulation of microvascular resistance in vivo. 体内微血管阻力的流速依赖性调节。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-12-01

A Koller, G Kaley

引用次数: 0

Microvascular perfusion during atrial pacing in renal hypertension induced cardiac hypertrophy. 肾性高血压患者心房起搏期间微血管灌注引起的心脏肥厚。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-12-01

C M Cimini, H R Weiss

{"title":"Microvascular perfusion during atrial pacing in renal hypertension induced cardiac hypertrophy.","authors":"C M Cimini, H R Weiss","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of this study was to determine if microvascular reserve was recruited during atrial pacing in the hypertrophied myocardium. Hypertrophy was induced by one-kidney, one-clip (1K1C) renal hypertension and compared to uninephrectomized control rabbits 30 days after surgery. Coronary flow was determined by radioactive microspheres. Microvascular perfusion was determined by comparison of fluorescein isothiocyanate-dextran labeled vessels with an alkaline phosphatase stain. Heart rates were not different between groups and all animals were paced 35% above baseline. Baseline coronary flow (176 +/- 44 and 207 +/- 61 ml/min/100 g, control and 1K1C animals) was not altered by pacing in either group. The baseline percent of capillaries perfused was not different between groups (56 +/- 2 and 61 +/- 3%, sham and 1K1C) and the percent perfused increased significantly during pacing for both the non-hypertrophied and 1K1C myocardium (76 +/- 6 and 78 +/- 10%). The baseline percent of the arteriolar bed perfused, was higher in the 1K1C (86 +/- 5%) compared to non-hypertrophied (63 +/- 6%) myocardium. During pacing, the percent of the arteriolar bed perfused increased in non-hypertrophied (89 +/- 14%) but not in the 1K1C myocardium. Although the percent of arterioles perfused did not increase with pacing in the 1K1C myocardium, the capillary reserve was recruited, facilitating the transport of O2 in both the hypertrophied and non-hypertrophied myocardium.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"5 6","pages":"457-84"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13679519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of ischemia-reperfusion derived oxygen free radicals on skeletal muscle calcium metabolism. 缺血再灌注源性氧自由基对骨骼肌钙代谢的影响。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-06-01

J L Cronenwett, K R Lee, M Shlafer, G B Zelenock

{"title":"The effect of ischemia-reperfusion derived oxygen free radicals on skeletal muscle calcium metabolism.","authors":"J L Cronenwett, K R Lee, M Shlafer, G B Zelenock","doi":"","DOIUrl":"","url":null,"abstract":"This study investigated the contribution of cytotoxic oxygen-derived free radicals to the skeletal muscle injury seen in a rat hindlimb tourniquet model after ischemia and reperfusion. The free radical scavengers superoxide dismutase (SOD) and catalase (CAT) were used as biologic probes to detect free radical activity, while Ca2+ uptake by sarcoplasmic reticulum (SR) was used to measure subcellular muscle function. Anesthetized rats received SOD (2 mg/kg IV) plus CAT (3.5 mg/kg IV, n = 6 treated group) or saline alone (4 ml/kg, n = 6 control group) 5 min before unilateral hindlimb tourniquet ischemia of 3 hr duration. SOD and CAT were conjugated to polyethylene glycol to increase their plasma half-life. After 19 hr reperfusion, muscle from ischemic and non-ischemic lower legs of each rat was excised and homogenized. Skeletal muscle SR was isolated by differential centrifugation and ATP-dependent Ca2+ uptake by SR was then measured with dual wavelength spectrophotometry and a calcium-sensitive dye. In control rats, Ca2+ uptake velocity by SR from ischemic muscle was reduced by 48% compared with contralateral non-ischemic muscle (p less than .001). Rats pretreated with SOD + CAT showed a less severe (27%) reduction in Ca2+ uptake velocity by SR from ischemic muscle. Thus, SOD + CAT significantly (p less than .01) reduced the dysfunction of SR Ca2+ transport seen in this tourniquet ischemia model. These results strongly implicate the involvement of oxygen-derived free radicals in abnormal Ca2+ transport observed in skeletal muscle after ischemia and reperfusion.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"5 3-5","pages":"171-87"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13778672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of heparin in reducing skeletal muscle infarction in ischemia-reperfusion. 肝素在减少缺血再灌注骨骼肌梗死中的作用。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-06-01

R W Hobson, R Neville, B Watanabe, J Canady, J G Wright, M Belkin

{"title":"Role of heparin in reducing skeletal muscle infarction in ischemia-reperfusion.","authors":"R W Hobson, R Neville, B Watanabe, J Canady, J G Wright, M Belkin","doi":"","DOIUrl":"","url":null,"abstract":"Heparin continues to be recommended in the clinical management of limb ischemia to prevent extension of distal vascular thrombosis and increased rates of limb loss. However, heparin may also be responsible for reduced skeletal muscle injury. Although its mechanism of action has not been fully evaluated, we have investigated the ability of heparin to minimize skeletal muscle injury associated with the ischemia-reperfusion syndrome in an in vivo canine gracilis muscle model. Our findings demonstrated a significant reduction in the amount of skeletal muscle infarction, microvascular permeability, and H+ ion accumulation cumulation after preischemic heparinization. Diffuse intravascular coagulation also has been observed in observed in this model which may be prevented or reduced by the anticoagulant properties of heparin when administered prior to ischemia. However, heparin's protective effect may be independent of its anticoagulant activity. Heparin is a polycomponent drug with non-anticoagulant properties which may serve to reduce cellular injury during ischemia and reperfusion in several different ways. Microvascular injury is decreased by the restoration of normal intimal negative charge and through the binding and resultant inactivation of histamine, bradykinin and other vasoactive amines. Heparin inhibits the complement cascade which is known to determine ischemic infarct size. Other factors of importance in determining the extent of skeletal injury include neutrophil activation, chemotaxis, enzyme release, and free oxygen radical generation, all of which are decreased or modulated by heparin. Heparin is a complex substance and much more remains to be learned about its anticoagulant and nonanticoagulant properties as well as its protective effects on skeletal muscle injury in ischemia-reperfusion syndrome.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"5 3-5","pages":"259-76"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13778676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Symposium on ischemia-reperfusion in skeletal muscle. Toronto, September 10, 1989. Proceedings. 骨骼肌缺血再灌注专题讨论会。多伦多，1989年9月10日。程序。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-06-01

引用次数: 0

Mechanisms of cellular injury: potential sources of oxygen free radicals in ischemia/reperfusion. 细胞损伤机制:缺血/再灌注中氧自由基的潜在来源。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-06-01

W Inauen, M Suzuki, D N Granger

引用次数: 0

The role of fibrinolysis during reperfusion of ischemic skeletal muscle. 纤维蛋白溶解在缺血骨骼肌再灌注中的作用。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-06-01

W J Quiñones-Baldrich

{"title":"The role of fibrinolysis during reperfusion of ischemic skeletal muscle.","authors":"W J Quiñones-Baldrich","doi":"","DOIUrl":"","url":null,"abstract":"Skeletal muscle is unique in its ability to tolerate relatively long periods of ischemia without demonstrable damage following reperfusion. Prolonged ischemia, however, has been associated with muscle necrosis and poor recovery of function. Using a rabbit model of hind limb ischemia, periods of ischemia of 1, 2, 3, and 5 hours were studied. Whereas almost complete recovery was seen after 1 or 2 hours of ischemia, a progressive loss of function is seen with increasing ischemic interval. In addition, within the 5 hour group, up to 40% of preparations did not recover function during reperfusion, with no Doppler signals audible over the pedicle. In these, microscopic thrombi was demonstrated histologically. Thus it appears that the \"no reflow\" phenomenon plays a major role after prolonged (greater than 4 hrs) ischemia. In order to evaluate the effect of fibrinolytic drugs on the \"no reflow\" phenomenon, urokinase was infused prior to reperfusion, and after 5 hours of ischemia, in a separate group of animals. All of these reperfused without any evidence of \"no reflow\". We conclude that reperfusion injury may have two major components: the \"no reflow\" phenomenon secondary to poor reperfusion, and cellular injury resulting from reperfusion itself. Infusion of fibrinolytic agents during the initial phases of reperfusion may have a salutory effect in preventing the \"no reflow\" phenomenon. It is likely, however, that attempts at effective and safe retrieval of ischemic tissue will necessarily have to address both mechanisms.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"5 3-5","pages":"299-314"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13778677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0