Microcirculation, endothelium, and lymphatics最新文献_第6页

Morphometric changes in pericyte-capillary endothelial cell associations correlated with vasoactive stimulus. 周细胞-毛细血管内皮细胞的形态变化与血管活性刺激相关。

Microcirculation, endothelium, and lymphatics Pub Date : 1990-04-01

R A Buchanan, R C Wagner

引用次数: 0

Evaluation of membrane fluidity in cerebral microvasculature. 脑微血管膜流动性的评价。

Microcirculation, endothelium, and lymphatics Pub Date : 1990-02-01

A Villacara, G Zanchin, M Spatz

引用次数: 0

Atrial natriuretic peptide increases microvascular blood flow and macromolecular escape during renin infusion in the hamster. 心房利钠肽增加仓鼠肾素输注时微血管血流和大分子逃逸。

Microcirculation, endothelium, and lymphatics Pub Date : 1990-02-01

M P Borić, R Albertini

{"title":"Atrial natriuretic peptide increases microvascular blood flow and macromolecular escape during renin infusion in the hamster.","authors":"M P Borić, R Albertini","doi":"","DOIUrl":"","url":null,"abstract":"The effects of Atrial Natriuretic Peptide (ANP) on microvascular hemodynamics and macromolecular permselectivity were studied in the hamster cheek pouch under resting conditions and during intravenous renin infusion. Fluorescent intravital microscopy was used to observe arteriolar diameters and to detect escape of fluorescent dextran of 150 K-Daltons (FITC-Dx-150). Microvascular plasma flow was estimated by clearance of 51Cr-EDTA and net macromolecular transport by clearance of FITC-Dx-150. At rest, topical ANP (2-250 ng/ml) had no effect on arteriolar diameter, 51Cr-EDTA clearance, relative vascular conductance (RVC) or FITC-Dx-150 clearance. Infusion of renin (10 mU/Kg/Hr, iv) elevated systemic arterial pressure by 30% and reduced cheek pouch RVC by 26%. During renin infusion, topical ANP (50 ng/ml) produced transient arteriolar vasodilation, and increased 51Cr-EDTA clearance (+35%), RVC (+58%) and FITC-Dx-150 clearance (+54%), without affecting systemic pressure. ANP did not induce venular leakage sites under any condition, but changes in FITC-Dx-150 clearance were highly correlated with changes in 51Cr-EDTA clearance, suggesting that the larger macromolecular escape was due to increases in microvascular blood flow and capillary/post-capillary hydrostatic pressure.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"6 1","pages":"67-88"},"PeriodicalIF":0.0,"publicationDate":"1990-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12858597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between blood flow, development of edema and leukocyte accumulation in post-ischemic rat skeletal muscle. 缺血后大鼠骨骼肌血流、水肿发展与白细胞积累的关系。

Microcirculation, endothelium, and lymphatics Pub Date : 1990-02-01

A Sirsjö, P Söderkvist, U Gustafsson, D H Lewis, G Nylander

{"title":"The relationship between blood flow, development of edema and leukocyte accumulation in post-ischemic rat skeletal muscle.","authors":"A Sirsjö, P Söderkvist, U Gustafsson, D H Lewis, G Nylander","doi":"","DOIUrl":"","url":null,"abstract":"The relationship between blood flow (xenon washout method), edema formation (percent total water content), and the number of polymorphonuclear leukocytes (PMNLs), as measured by the level of the enzyme myeloperoxidase, has been investigated in post-ischemic skeletal muscle of rats. A tourniquet model of temporary, complete ischemia of one hindlimb for 3 or 4 hours was used. Biopsies were taken after 0.5, 5 and 12 hours of reperfusion (6 experimental groups) and from a control group that had received only anesthesia. After 4 hours, but not 3 hours of ischemia there was a restricted blood flow during the early reperfusion phase, the \"no-reflow\" phenomenon, indicating severe ischemia. There was no significant accumulation of PMNLs in the skeletal muscle nor was there a correlation between the number of PMNLs in the post-ischemic muscle and the restricted bloodflow. With 4 hours of ischemia and 0.5 hours of reperfusion there was a statistically significant, positive correlation between the number of PMNLs and the amount of edema; no such correlation was evident in either of the other groups. These results suggest that PMNLs are not the major cause of reduced bloodflow or of edema in the early reperfusion phase after total ischemia.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"6 1","pages":"21-34"},"PeriodicalIF":0.0,"publicationDate":"1990-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13317052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total red cell Ca2+ content and rheological determinants in a group of subjects with vascular atherosclerotic disease. 血管粥样硬化性疾病患者的红细胞总Ca2+含量和流变学决定因素

Microcirculation, endothelium, and lymphatics Pub Date : 1990-02-01

G Caimi, R Lo Presti, A Serra, G Grifò, A Romano, A Catania, A Sarno

引用次数: 0

Stereospecificity of the anti-inflammatory actions of terbutaline. 特布他林抗炎作用的立体特异性。

Microcirculation, endothelium, and lymphatics Pub Date : 1990-02-01

D E Dobbins, M J Buehn, J M Dabney

{"title":"Stereospecificity of the anti-inflammatory actions of terbutaline.","authors":"D E Dobbins, M J Buehn, J M Dabney","doi":"","DOIUrl":"","url":null,"abstract":"Intra-arterial infusion of a racemic mixture of the beta 2-agonist terbutaline blocks histamine-mediated increases in lymph flow and protein concentration in the canine forelimb. In the current study we have assessed the relative anti-inflammatory potencies of the purified stereoisomers of terbutaline. Infusion of histamine (4 micrograms base/min) increased lymph flow, protein concentration and protein transport. The intra-arterial infusion of 1-terbutaline (1 microgram/min) significantly decreased forelimb arterial pressures and prevented any changes in lymph parameters due to subsequent histamine infusion. Intra-arterial infusion of d-terbutaline (1 microgram/min) did not significantly affect forelimb vascular pressures but subsequent to histamine administration, lymph parameters increased similar to that seen with histamine alone. Infusion of a high dose of d-terbutaline (100 micrograms/min) slightly decreased forelimb arterial pressures but failed to inhibit histamine-mediated increases in lymph parameters. Infusion of 1-terbutaline alone (1 microgram/min) significantly decreased forelimb arterial pressures, lymph flow and protein transport and slightly but significantly increased lymph protein concentration. These data indicate that the beta 2-agonistic and anti-inflammatory properties of terbutaline are confined solely to the levorotatory enantiomer.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"6 1","pages":"3-20"},"PeriodicalIF":0.0,"publicationDate":"1990-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13504675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A study of platelet fibronectin immunofluorescence in peripheral vascular disease and diabetes mellitus. 血小板纤维连接蛋白免疫荧光在外周血管疾病和糖尿病中的研究。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-12-01

O S Ejim, M A Barradas, D P Mikhailidis, L W Poulter, A Coumar, P Dandona

{"title":"A study of platelet fibronectin immunofluorescence in peripheral vascular disease and diabetes mellitus.","authors":"O S Ejim, M A Barradas, D P Mikhailidis, L W Poulter, A Coumar, P Dandona","doi":"","DOIUrl":"","url":null,"abstract":"Platelets hyperaggregability and hypersecretion fibronectin (Fn) are known to occur in peripheral vascular disease (PVD) and diabetes mellitus (DM) with microangiopathy. To determine whether an increase in platelet membrane bound Fn constitutes to hyperaggregability of platelets, washed platelets from normal subjects and from patients with peripheral vascular disease and patients with diabetes mellitus were examined for the presence of fibronectin (Fn) by means of fluorescein linked antibody to Fn. Platelets from peripheral vascular disease and diabetes mellitus patients tended to aggregate during preparation and apparently exhibited greater fluorescence in platelet \"smears\" than was observed in smears from controls. In contrast, when washed platelet \"smears\" were prepared from platelet preparations containing iloprost, an analogue of prostacyclin, platelet aggregates did not form and the 'excess' of fluorescence disappeared from all the three groups. When platelets were stained for Fn fluorescence in suspensions, no fluorescence was observed on the surface of platelets from peripheral vascular disease and diabetes mellitus patients or controls. On stimulation with thrombin washed platelet suspension showed fluorescence for Fn. Platelet activation leads to Fn appearing on platelet surface but this effect cannot be quantified by optical fluorescence microscopy.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"5 6","pages":"373-90"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13628995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Separate effects of gravity and venous pressure on regional and capillary blood flows in the human finger. 重力和静脉压对手指局部和毛细血管血流的单独影响。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-12-01

D R Richardson, S Shepherd

{"title":"Separate effects of gravity and venous pressure on regional and capillary blood flows in the human finger.","authors":"D R Richardson, S Shepherd","doi":"","DOIUrl":"","url":null,"abstract":"The objective of this study was to determine if and to what extent a veno-arteriolar reflex is responsible for the adjustments in regional and capillary blood flows that occur in response to gravitational stress in the human finger. Nine male subjects 20 to 40 years of age consented to have regional cutaneous blood flow measured in the index finger via a laser Doppler flowmeter (LDF) and blood cell velocity (CBV) measured in individual nailfold capillaries via video microscopy while placing the hand 20 then 40 cm below the heart and while a pneumatic cuff placed around the wrist was inflated to pressures of 20, 40 than 60 mm Hg. Both lowering the hand and selective elevation of venous pressure elicited significant decreases in LDF and CBV (P less than 0.03). The flow reductions that occurred with lowering the hand 20 cm below the heart were significantly greater (P less than 0.05) for both LDF and CBV when compared to cuff pressure elevation of 27 cm H2O (20 mm Hg), and significantly greater (P less than 0.095) for LDF in matching the 40 cm below the heart position to data obtained at a cuff pressure of 57 cm H2O (40 mm Hg). Analyzing the flow responses relative to precapillary perfusion pressure (arterial pressure - estimated capillary pressure) indicated the reductions in LDF and CBV that occurred in response to cuff pressure elevation were a passive effect of the increase in venous pressure itself. These results indicate that the reductions in regional and capillary blood flow that occur in response to gravitational stress in the fingers are due to myogenic vasoconstriction of arterioles secondary to a rise in arterial pressure and that a veno-arteriolar reflex mechanism is not operative in this region of the cutaneous circulation.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"5 6","pages":"417-33"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13775820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chicago sky blue and a helium neon laser abolish endothelium dependent relaxation in vivo in the microcirculation. 芝加哥天蓝和氦氖激光消除体内微循环中内皮依赖性松弛。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-12-01

H Nishimura, G H Nelson, W I Rosenblum

引用次数: 0

Microvascular effects of endothelin-1. 内皮素-1的微血管作用。

Microcirculation, endothelium, and lymphatics Pub Date : 1989-12-01

E J Messina, J Rodenburg, G Kaley

{"title":"Microvascular effects of endothelin-1.","authors":"E J Messina, J Rodenburg, G Kaley","doi":"","DOIUrl":"","url":null,"abstract":"In the present study we examined the microvascular actions of endothelin (ET-1) in rat cremaster arterioles. Pentobarbital (35 mg/kg) anesthetized, five to six week old rats were prepared for in vivo observation and measurement of microvascular control dimensions and changes in diameter, in response to the topical administration of ET-1 (1 x 10(-11) to 5 x 10(-10) M). The effects of ET-1 were determined before and after the administration, by suffusion upon the cremaster muscle, of either indomethacin (IND, 10 micrograms/ml) or methylene blue (MB, 5 microM). Third order arterioles; 13-25 microns in diameter, were selected for study. The administration of ET-1 evoked a biphasic response of cremaster arterioles, an initial small, fleeting increase in diameter (14 to 26%) followed by a dose-dependent, longer lasting decrease in diameter (20 to 77%). The administration of either MB or IND had no effect on the arteriolar dilator and constrictor responses to ET-1. These results suggest that ET-1 is more potent a constrictor than dilator agent in this vascular bed and that the dilator component of the response is not mediated by either prostaglandins or EDRF. By virtue of its actions and potency, we conclude that ET may be an important factor in the regulation of vascular tone and local blood flow.","PeriodicalId":18718,"journal":{"name":"Microcirculation, endothelium, and lymphatics","volume":"5 6","pages":"505-18"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13839630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0