Microbial EcologyPub Date : 2025-09-25DOI: 10.1007/s00248-025-02596-z
Shengjie Sun, Zhiyi Qiao, Denis V Tikhonenkov, Yingchun Gong, Hua Li, Renhui Li, Kexin Sun, Da Huo
{"title":"Temporal Dynamics and Adaptive Mechanisms of Microbial Communities: Divergent Responses and Network Interactions.","authors":"Shengjie Sun, Zhiyi Qiao, Denis V Tikhonenkov, Yingchun Gong, Hua Li, Renhui Li, Kexin Sun, Da Huo","doi":"10.1007/s00248-025-02596-z","DOIUrl":"10.1007/s00248-025-02596-z","url":null,"abstract":"<p><p>Microbial communities are vital to aquatic ecosystems, driving biogeochemical cycles, nutrient recycling, and overall ecosystem functioning. However, their instant feedback, particularly in response to environmental fluctuations, remain insufficiently understood. In this study, we investigated the interaction of prokaryotic and eukaryotic microbial communities in riverine ecosystems under temporal dynamics using high-throughput sequencing and co-occurrence network analysis. We observed distinct patterns, with eukaryotic communities showing a consistent increase in alpha diversity, while prokaryotic communities exhibited more variable and directional shifts over time. Two key phases were identified: a dynamic phase characterized by rapid changes in both alpha and beta diversity and a stabilization phase where community composition became more stable, with increased evenness. Co-occurrence network analysis revealed a transition from a modular structure in the dynamic phase to a more centralized and highly connected network in the stabilization phase. While modularity can enhance stability by localizing perturbations within distinct subnetworks, increased centralization and connectivity may weaken this stabilizing effect, potentially making the network less resilient to environmental fluctuations. Our findings provide new insights into the adaptive mechanisms that sustain microbial community stability and resilience in dynamic aquatic ecosystems, emphasizing the importance of diversity and network structure in maintaining ecological stability.</p>","PeriodicalId":18708,"journal":{"name":"Microbial Ecology","volume":"88 1","pages":"94"},"PeriodicalIF":4.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial EcologyPub Date : 2025-09-02DOI: 10.1007/s00248-025-02593-2
D Alex R Gordon, David J Burke, Sarah R Carrino-Kyker, Claudia Bashian-Victoroff, Adam I Mabrouk, John T Van Stan
{"title":"Community Composition of Microbial Eukaryotes Transported by Stemflow from Fagus grandifolia Ehrh. (American Beech) Trees in Northeastern Ohio (USA).","authors":"D Alex R Gordon, David J Burke, Sarah R Carrino-Kyker, Claudia Bashian-Victoroff, Adam I Mabrouk, John T Van Stan","doi":"10.1007/s00248-025-02593-2","DOIUrl":"10.1007/s00248-025-02593-2","url":null,"abstract":"<p><p>Stemflow, the concentrated fraction of rainfall that drains down tree trunks, can translocate canopy biota to the forest floor, but its eukaryotic composition remains uncharacterized via eDNA methods. We collected stemflow from 18 Fagus grandifolia (American beech) trees during ten storms in northeastern Ohio (USA) and analyzed 18S rRNA eDNA to resolve transported microbial-eukaryote communities. Over 12 million reads (83 samples) revealed 920 zero-radius OTUs spanning fungi, algae, protists, and metazoans. Community composition differed significantly among storm events (PERMANOVA F = 3.6, r<sup>2</sup> = 0.31, p < 0.001) and among NOAA HYSPLIT modeled air-mass back-trajectories (F = 8.9, r<sup>2</sup> = 0.36, p < 0.001). Summer storms were dominated by fungal taxa (Entomophthoromycota, Basidiomycota, and Ascomycota comprised up to 90% of reads), whereas late-autumn and winter storms carried mainly algal stramenopiles (Ochrophyta). Large storms (> 60 mm event<sup>-1</sup>) mobilized conspicuously higher relative abundances of larger metazoans (tardigrades and arthropods). We infer from stemflow eDNA that (i) seasonal resource shifts in tree canopies favor parasitic fungi in summer and saprotrophic fungi in autumn; (ii) northerly winter storms entrain Great Lakes aerosol algae that deposit onto canopies; (iii) rainfall intensity and duration jointly control the detachment of well-attached canopy eukaryotes. Together, our results establish stemflow eDNA as a non-invasive window into storm-mediated linkages between above- and below-ground biodiversity, offering new scope for monitoring canopy microbiomes under intensifying hydro-climatic regimes.</p>","PeriodicalId":18708,"journal":{"name":"Microbial Ecology","volume":"88 1","pages":"93"},"PeriodicalIF":4.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial EcologyPub Date : 2025-08-29DOI: 10.1007/s00248-025-02592-3
Xinli Sun, Riyan Xia, Jiyu Xie, Kun Duan, Weibing Xun, Nan Zhang, Guidong Huang, Ruifu Zhang, Qirong Shen, Kai Wu, Zhihui Xu
{"title":"Cooperative Interactions Between Bacillus and Lysobacter Enhance Consortium Stability and Fusarium Wilt Suppression in Cucumber.","authors":"Xinli Sun, Riyan Xia, Jiyu Xie, Kun Duan, Weibing Xun, Nan Zhang, Guidong Huang, Ruifu Zhang, Qirong Shen, Kai Wu, Zhihui Xu","doi":"10.1007/s00248-025-02592-3","DOIUrl":"https://doi.org/10.1007/s00248-025-02592-3","url":null,"abstract":"<p><p>The rhizosphere microbiome plays a pivotal role in plant health by mediating interactions between hosts, beneficial microbes, and pathogens. However, the ecological mechanisms underlying microbial consortia that suppress soil-borne diseases remain largely unexplored. In this study, we investigated how the biocontrol bacterium Bacillus velezensis SQR9 influences the assembly of the cucumber rhizosphere bacterial community in the presence of the pathogenic fungus Fusarium oxysporum f. sp. cucumerinum (FOC). Inoculation with B. velezensis SQR9 significantly enriched the genus Lysobacter, a known biocontrol taxon. Meta-analysis revealed a positive correlation between Bacillus and Lysobacter abundances in healthy plant rhizospheres-a relationship absent in Fusarium wilt-diseased soils-suggesting a conserved ecological association linked to disease suppression. Mechanistic assays demonstrated that Lysobacter enzymogenes XL8, an antifungal bacterium isolated from the cucumber rhizosphere, formed synergistic biofilms with B. velezensis SQR9. Spent medium growth assays indicated that strain SQR9 facilitated the growth of L. enzymogenes XL8 through metabolic interactions. Targeted RT-qPCR and UHPLC-MS/MS analyses confirmed that treatment with spent medium of the partner strain enhanced the expression and production of antifungal metabolites bacillomycin D and heat-stable antifungal factor (HSAF), both antagonistic to F. oxysporum. Greenhouse trials confirmed that this dual-species consortium more effectively suppressed Fusarium wilt than single-species inoculations, as evidenced by reduced pathogen abundance and enhanced plant growth. Together, our findings underscore the importance of microbial metabolic cooperation and biofilm-mediated coexistence in shaping rhizosphere community assembly and function, providing ecological insights for the development of synthetic microbial consortia aimed at sustainable plant disease management.</p>","PeriodicalId":18708,"journal":{"name":"Microbial Ecology","volume":"88 1","pages":"92"},"PeriodicalIF":4.0,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12397161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial EcologyPub Date : 2025-08-28DOI: 10.1007/s00248-025-02588-z
Qianwen Shao, Qingchuan Yang, Yuankai Xu, Ling Zhang, Ming Ding, Fan Li, Congying He
{"title":"The Impact of Artificial Water Diversion-induced Algal Blooms on Carbon Balance in a Semi-enclosed Bay.","authors":"Qianwen Shao, Qingchuan Yang, Yuankai Xu, Ling Zhang, Ming Ding, Fan Li, Congying He","doi":"10.1007/s00248-025-02588-z","DOIUrl":"https://doi.org/10.1007/s00248-025-02588-z","url":null,"abstract":"<p><p>Artificial water diversion is widely used to address water security; yet, its impacts on phytoplankton communities and coastal carbon balance remain poorly understood. Using a seasonal diversion project in a semi-enclosed bay as a case study, we analyzed phytoplankton composition via morphological methods and assessed carbon balance through simultaneous measurements of primary production (P), ecosystem respiration rate (R), and production-to-respiration (PP/R) ratio. Our results showed that artificial water diversion activities during the wet month enhanced hydrological connectivity and phytoplankton homogeneity, triggering a mixed diatom-dinoflagellate bloom. Phytoplankton abundance during the wet month increased by sevenfold (surface layer) and 26.5-fold (bottom layer) compared to dry month values. This simultaneously resulted in the PP value of the wet month being more than twice that of the dry month. Although R rose with increasing phytoplankton abundance, no significant correlation was observed between them. Instead, dry-month R was primarily driven by pH and dissolved organic carbon, whereas wet-month R showed minimal environmental linkages. PP/R ratios of surface and bottom layers were always less than 1, implying Meishan bay was a net heterotrophic ecosystem, despite significant changes in phytoplankton community structure induced by artificial water diversion and associated algal bloom. Furthermore, our results strongly suggest that changes in PP, but not in R, control the PP/R ratio of Meishan bay. This study offers valuable guidance for the ecological management of artificial water diversions and can serve as a reference for similar water diversion projects in other semi-enclosed bays.</p>","PeriodicalId":18708,"journal":{"name":"Microbial Ecology","volume":"88 1","pages":"91"},"PeriodicalIF":4.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial EcologyPub Date : 2025-08-26DOI: 10.1007/s00248-025-02589-y
Ekaterina Pushkareva, Leonie Keilholz, Justin Böse, Karl-Heinz Linne von Berg
{"title":"Genetic Diversity and Potential of Cyanobacteria and Fungi Living on Arctic Liverworts.","authors":"Ekaterina Pushkareva, Leonie Keilholz, Justin Böse, Karl-Heinz Linne von Berg","doi":"10.1007/s00248-025-02589-y","DOIUrl":"https://doi.org/10.1007/s00248-025-02589-y","url":null,"abstract":"<p><p>Liverworts often form symbiotic associations with fungi and cyanobacteria, yet the distribution and specificity of these relationships remain largely unexplored, particularly in Arctic environments. This study used metagenomic sequencing to investigate fungal and cyanobacterial communities associated with Arctic liverworts, analyzing photosynthetic parts of gametophytes and their rhizoids with attached soil separately. The results revealed that Ascomycota dominated the fungal community. The cyanobacterial community was primarily composed of heterocytous Nostoc and non-heterocytous filamentous Leptolyngbya, with Nostoc showing evidence of nitrogen fixation, especially in gametophytes, suggesting a potential role in enhancing nitrogen availability for the host. These findings underscore the ecological significance of liverwort-associated microorganisms in Arctic ecosystems, with microbial composition differing between upper and lower parts of plants, as well as between leafy and thalloid liverworts, indicating possible functional specialization.</p>","PeriodicalId":18708,"journal":{"name":"Microbial Ecology","volume":"88 1","pages":"90"},"PeriodicalIF":4.0,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacillus subtilis ED24 Controls Fusarium culmorum in Wheat Through Bioactive Metabolite Secretion and Modulation of Rhizosphere Microbiome.","authors":"Fatima Ezzahra Oulkhir, Abdelmounaaim Allaoui, Ayoub Idbella, Mohammed Danouche, Adnane Bargaz, Latefa Biskri, Mohamed Idbella","doi":"10.1007/s00248-025-02590-5","DOIUrl":"10.1007/s00248-025-02590-5","url":null,"abstract":"<p><p>Fusarium culmorum is a soil-borne fungal pathogen causing root and stem rot, seedling blight, and significant yield losses in small grain cereals, including wheat. This study aimed to evaluate the antifungal potential of Bacillus subtilis ED24, an endophytic strain isolated from Ziziphus lotus (L.) roots, and its effects on wheat growth and yield under controlled conditions. In vitro assays demonstrated that B. subtilis ED24 inhibited F. culmorum mycelial growth by up to 87%, associated with the secretion of 37 distinct secondary metabolites, predominantly involved in carbon cycling. In pot experiments, B. subtilis ED24 significantly enhanced wheat germination (85%) and growth compared to infected plants treated with the chemical fungicide tebuconazole. Although nutrient analysis showed significantly higher shoot nitrogen (32.34 mg/pot) and phosphorus (2.41 mg/pot) contents in the B. subtilis ED24 treatment compared to tebuconazole (8.11 and 0.18 mg/pot, respectively), no significant differences were observed when compared to the infected control (C-). Similarly, B. subtilis ED24 led to improved thousand grain weight (40.4 g), protein content (19.98%), and ash content (1.95%) relative to tebuconazole (29.1 g, 18.31%, and 1.74%, respectively), yet these values did not differ significantly from the infected control (C-). Notably, the number of seeds per pot was significantly increased by B. subtilis ED24 compared to the infected control (C-) (113.8 seeds/pot vs. 54.2 seeds/pot). Additionally, B. subtilis ED24 modulated the wheat rhizosphere microbiome, enriching beneficial taxa such as Eurotiomycetes fungal class and the bacterial genus Paramesorhizobium. These findings suggest that the antifungal activity and growth-promoting effects of B. subtilis ED24 are likely mediated through the synthesis of unique bioactive metabolites and microbiome modulation, offering a promising sustainable alternative to chemical fungicides in wheat production.</p>","PeriodicalId":18708,"journal":{"name":"Microbial Ecology","volume":"88 1","pages":"89"},"PeriodicalIF":4.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12364970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Responses of the Coral Symbiont Cladocopium goreaui to Extreme Temperature Stress in Relatively High-Latitude Reefs, South China Sea.","authors":"Lifei Wei, Shuchang Chen, Zhenjun Qin, Nengbin Pan, Mengling Lan, Tingchao Zhang, Ran He, Hongye Liang, Wenzhi Deng, Changhao Mo, Kefu Yu","doi":"10.1007/s00248-025-02587-0","DOIUrl":"10.1007/s00248-025-02587-0","url":null,"abstract":"<p><p>Global climate change has led to frequent extreme temperature events in oceans. Corals are susceptible to extreme high-temperature stress in summer and extreme low-temperature stress in winter in the relatively high-latitude reef areas of the South China Sea (SCS). The most abundant symbiotic coral Symbiodiniaceae in the higher-latitude reefs of the SCS is Cladocopium goreaui, predominantly associating with dominant coral hosts such as Acropora and Porites. However, to date, relatively few studies have focused on the response and mechanism of C. goreaui to the extreme high- and low-temperature stress. In this study, the responses and regulatory mechanisms of the dominant C. goreaui to extreme high- and low-temperature stress were investigated based on physiological indexes, transmission electron microscopy (TEM), and transcriptome analysis. The results showed that (1) under 34 °C heat stress, the disintegration of thylakoids triggered photosynthetic collapse in C. goreaui; survival is enabled through metabolic reprogramming that upregulates five protective pathways and redirects energy via pentose/glucuronate shunting to sustain ATP homeostasis, revealing a trade-off between damage containment and precision energy governance under thermal extremes. (2) Low temperature exposure induced suppression of maximum quantum yield (F<sub>v</sub>/F<sub>m</sub>), compounded by glutathione pathway inhibition, crippling ROS scavenging. The transcriptome results revealed that C. goreaui prioritizes gene fidelity maintenance under low temperature stress. These findings reveal that energy allocation trade-offs constitute the core strategy of C. goreaui temperature response: prioritizing energy maintenance under high-temperature stress, while safeguarding genetic fidelity at the expense of antioxidant defense under low-temperature stress.</p>","PeriodicalId":18708,"journal":{"name":"Microbial Ecology","volume":"88 1","pages":"88"},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12343640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial EcologyPub Date : 2025-08-08DOI: 10.1007/s00248-025-02586-1
Jiyeon Sung, Dong Han Choi, Yeonjung Lee, Joo-Hwan Kim, Hyeon Ho Shin, Young-Eun Kim, Jae Ho Choi, Jae Hoon Noh, Christopher J Gobler, Bum Soo Park
{"title":"Temperature-Driven Intraspecific Diversity in Paralytic Shellfish Toxin Profiles of the Dinoflagellate Alexandrium pacificum and Intragenic Variation in the Saxitoxin Biosynthetic Gene, sxtA4.","authors":"Jiyeon Sung, Dong Han Choi, Yeonjung Lee, Joo-Hwan Kim, Hyeon Ho Shin, Young-Eun Kim, Jae Ho Choi, Jae Hoon Noh, Christopher J Gobler, Bum Soo Park","doi":"10.1007/s00248-025-02586-1","DOIUrl":"10.1007/s00248-025-02586-1","url":null,"abstract":"<p><p>Alexandrium pacificum, a globally distributed dinoflagellate, is well-known for causing harmful algal blooms and producing Paralytic Shellfish Toxins (PSTs), a threat to marine life and human health. The frequency and intensity of Alexandrium blooms have increased in recent decades, driven, in some cases, by increasing temperatures. Here, we investigated the temperature-dependent (15 °C, 20 °C, 25 °C, and 30 °C) growth rates and paralytic shellfish toxin profiles of eight A. pacificum strains while concurrently examining differences in sequences of the saxitoxin biosynthetic gene, sxtA4. While maximum cell densities were lowest at 30 °C, toxin production per cell was highest at higher temperatures that inhibited growth, with greater diversity of toxin analogs peaking at 30 °C, as confirmed by the higher Shannon's diversity index obtained for the toxin profiles with the increasing temperatures. Furthermore, genetic analysis of the sxtA4 gene showed that greater genetic diversity-quantified by nucleotide diversity (π) ranging from 9.91 to 30.21 across strains-was positively correlated with this wider array of toxin analogs (Shannon's diversity index; p < 0.0001). Conserved regions within the gene were identified, suggesting that these regions may play important structural or functional roles in the saxitoxin biosynthetic pathway. These findings highlight the role of temperature, genetic diversity, and sxtA4 conserved regions in influencing toxin production and profiles in Alexandrium. Further research into the genetic mechanisms underlying saxitoxin biosynthesis will improve our understanding of Alexandrium's adaptability to changing temperatures. Such insights are essential for effective ecosystem management and safeguarding public health.</p>","PeriodicalId":18708,"journal":{"name":"Microbial Ecology","volume":"88 1","pages":"87"},"PeriodicalIF":4.0,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial EcologyPub Date : 2025-08-06DOI: 10.1007/s00248-025-02573-6
Alison E Fowler, Quinn S McFrederick, Lynn S Adler
{"title":"Correction to: Pollen Diet Diversity does not Affect Gut Bacterial Communities or Melanization in a Social and Solitary Bee Species.","authors":"Alison E Fowler, Quinn S McFrederick, Lynn S Adler","doi":"10.1007/s00248-025-02573-6","DOIUrl":"10.1007/s00248-025-02573-6","url":null,"abstract":"","PeriodicalId":18708,"journal":{"name":"Microbial Ecology","volume":"88 1","pages":"86"},"PeriodicalIF":4.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12328505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial EcologyPub Date : 2025-08-02DOI: 10.1007/s00248-025-02580-7
R Cruz-Cano, L Bretón-Deval, M Martínez-García, P Díaz-Jaimes, M Kolb
{"title":"Changes in Microbial Community Assemblages Due To Urban Pollution, Detected via rRNA Gene Amplicon Sequencing in the Magdalena River, Mexico City.","authors":"R Cruz-Cano, L Bretón-Deval, M Martínez-García, P Díaz-Jaimes, M Kolb","doi":"10.1007/s00248-025-02580-7","DOIUrl":"10.1007/s00248-025-02580-7","url":null,"abstract":"<p><p>As freshwater sources become increasingly polluted and depleted, the delicate balance of aquatic environments is disrupted, leading to cascading effects throughout entire ecosystems. This disruption manifests in various ways, including changes in water chemistry, temperature fluctuations, and the introduction of contaminants, all of which contribute to alterations in microbial communities. We applied eDNA metabarcoding to characterize microbial communities along an anthropogenic pollution gradient in the Magdalena River, a tropical river in Mexico City. Sampling was conducted at four sites representing different levels of human influence. Results revealed differences in both bacterial and microeukaryotic community compositions between sites. In areas with low to moderate disturbance, bacterial genera associated with nitrogen cycling and plant-microbe interactions (e.g., Rhizobacter, Rhodoferax, and Flavobacterium) were predominant, whereas in more heavily impacted sites, genera linked to enteric, nosocomial, or fecal sources (e.g., Arcobacter, Acinetobacter, and Aeromonas) dominated. Peri-urban sites exhibited higher alpha diversity at the phylum level for bacteria, and microeukaryotic communities; two phyla account for over 75% of the relative abundance throughout the year (Ciliophora & Chlorophyta). Statistical analysis showed that water quality influences microbial composition in the sites. These findings demonstrate that urban influence alter microbial community composition, showing similar patterns to other studies. Our study, however, also discovered certain taxa that had not been previously recorded in tropical urban rivers, thereby broadening the existing knowledge, which has primarily been based on temperate systems. This research offers one of the initial thorough evaluations of microbial communities in urban rivers in Mexico and highlights the potential of eDNA metabarcoding as a valuable tool for environmental monitoring.</p>","PeriodicalId":18708,"journal":{"name":"Microbial Ecology","volume":"88 1","pages":"85"},"PeriodicalIF":4.0,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12317890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}