Molecular & general genetics : MGG最新文献_第8页

Pea Ty1-copia group retrotransposons: transpositional activity and use as markers to study genetic diversity in Pisum. 豌豆 Ty1-copia 组逆转录转座子：转座活动和用作研究豌豆遗传多样性的标记。

Molecular & general genetics : MGG Pub Date : 2000-07-01 DOI: 10.1007/s004380000257

S R Pearce, M Knox, T H Ellis, A J Flavell, A Kumar

引用次数: 130

Both sense and antisense RNAs are targets for the sense transgene-induced posttranscriptional silencing mechanism. 有义和反义 RNA 都是有义转基因诱导的转录后沉默机制的目标。

Molecular & general genetics : MGG Pub Date : 2000-07-01 DOI: 10.1007/pl00008700

H Van Houdt, M Van Montagu, A Depicker

引用次数: 29

Analysis of the expression of the Rhodobacter sphaeroides lexA gene. 球形红杆菌lexA基因的表达分析。

Molecular & general genetics : MGG Pub Date : 2000-07-01 DOI: 10.1007/pl00008696

A Tapias, S Campoy, J Barbé

{"title":"Analysis of the expression of the Rhodobacter sphaeroides lexA gene.","authors":"A Tapias, S Campoy, J Barbé","doi":"10.1007/pl00008696","DOIUrl":"https://doi.org/10.1007/pl00008696","url":null,"abstract":"The regulation of the Rhodobacter sphaeroides lexA gene has been analyzed using both gel-mobility experiments and lacZ gene fusions. PCR-mediated mutagenesis demonstrated that the second GAAC motif in the sequence GAACN7GAACN7GAAC located upstream of the R. sphaeroides lexA gene is absolutely necessary for its DNA damage-mediated induction. Moreover, mutagenesis of either the first or the third GAAC motif in this sequence reduced, but did not abolish, the inducibility of the R. sphaeroides lexA gene. A R. sphaeroides lexA-defective (Def) mutant has also been constructed by replacing the active lexA gene with an inactivated gene copy constructed in vitro. Crude extracts of the R. sphaeroides lexA(Def) strain are unable to form any protein-DNA complex when added to the wild-type lexA promoter of R. sphaeroides. Likewise, the R. sphaeroides lexA(Def) cells constitutively express the recA and lexA genes. All these data clearly indicate that the lexA gene product is the negative regulator of the R. sphaeroides SOS response. Furthermore, the morphology, growth and viability of R. sphaeroides lexA(Def) cultures do not show any significant change relative to those of the wild-type strain. Hence, R. sphaeroides is so far the only bacterial species whose viability is known not to be affected by the presence of a lexA(Def) mutation.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"263 6","pages":"957-65"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/pl00008696","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21791643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Identification and characterization of the mre gene region of Streptomyces coelicolor A3(2). colcolcolor链霉菌A3多基因区域的鉴定与鉴定(2)。

Molecular & general genetics : MGG Pub Date : 2000-07-01 DOI: 10.1007/s004380050034

A Burger, K Sichler, G Kelemen, M Buttner, W Wohlleben

{"title":"Identification and characterization of the mre gene region of Streptomyces coelicolor A3(2).","authors":"A Burger, K Sichler, G Kelemen, M Buttner, W Wohlleben","doi":"10.1007/s004380050034","DOIUrl":"https://doi.org/10.1007/s004380050034","url":null,"abstract":"During a search for new differentiation factors in Streptomyces coelicolor A3(2), a locus at 11 o'clock on the S. coelicolor map was identified which harbours several genes that show extensive similarity to cell division and differentiation genes from Escherichia coli and Bacillus subtilis. From the sequence data it was concluded that the region contains the genes mireB, mreC, mreD (murein formation gene cluster E), pbp83 (high-molecular-weight penicillin-binding protein) and sfr (member of the spoVE/ftsW/rodA family). Mre gene products are reported to be responsible for determining cell shape in E. coli and Bacillus. The S. coelicolor mreC gene was inactivated by gene disruption, resulting in mutants which showed significant growth retardation in comparison to the wild type. Inactivation of the mreB gene was incompatible with viability, and thus mreB represents a Streptomyces cell division gene that is essential for survival. Promoter-probe experiments led to the identification of an operon structure, with promoters located upstream of mreB, pbp83 and sfr. Detailed studies of mreB transcription revealed the existence of three promoters; two of them are constitutively transcribed, whereas the third is developmentally regulated.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"263 6","pages":"1053-60"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s004380050034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21790823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Disruption of plastid-encoded RNA polymerase genes in tobacco: expression of only a distinct set of genes is not based on selective transcription of the plastid chromosome. 烟草质体编码的 RNA 聚合酶基因的中断：只有一组不同基因的表达不是基于质体染色体的选择性转录。

Molecular & general genetics : MGG Pub Date : 2000-07-01 DOI: 10.1007/pl00008690

K Krause, R M Maier, W Kofer, K Krupinska, R G Herrmann

{"title":"Disruption of plastid-encoded RNA polymerase genes in tobacco: expression of only a distinct set of genes is not based on selective transcription of the plastid chromosome.","authors":"K Krause, R M Maier, W Kofer, K Krupinska, R G Herrmann","doi":"10.1007/pl00008690","DOIUrl":"10.1007/pl00008690","url":null,"abstract":"Plastids of higher plants operate with at least two distinct DNA-dependent RNA polymerases, which are encoded in the organelle (PEP) and in the nucleus (NEP), respectively. Plastid run-on assays and Northern analyses were employed to analyse gene expression in tobacco mutant plastids lacking the PEP genes rpoA, rpoB or rpoC1. Hybridisation of run-on transcripts to restriction fragments representing the entire tobacco plastid chromosome, as well as to selected plastid gene-specific probes, shows that all parts of the plastid DNA are transcribed in rpo-deficient plastids. In comparison to wild-type chloroplasts, which are characterized by preferential transcription of photosynthesis-related genes in the light, mutant plastids exhibit a different transcription pattern with less pronounced differences in the hybridisation intensities between the individual genes. The analysis of steady-state transcript patterns and transcription rates of selected genes in both types of plastids demonstrates that differences in transcription rates are not necessarily paralleled by corresponding changes in transcript levels. The accumulation of large transcripts in the mutant plastids indicates that processing of primary transcripts may be impaired in the absence of PEP. These data suggest that, contrary to the prevailing view, much of the regulation of NEP-driven plastid gene expression in the rpo-deficient mutants is not based on differential promoter usage but is exerted at post-transcriptional levels.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"263 6","pages":"1022-30"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/pl00008690","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21790919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 109

Members of the amylovora group of Erwinia are cellulolytic and possess genes homologous to the type II secretion pathway. 欧文氏菌淀粉菌群的成员具有纤维素分解能力，并具有与II型分泌途径同源的基因。

Molecular & general genetics : MGG Pub Date : 2000-07-01 DOI: 10.1007/pl00008691

R Riekki, T Palomäki, O Virtaharju, H Kokko, M Romantschuk, H T Saarilahti

{"title":"Members of the amylovora group of Erwinia are cellulolytic and possess genes homologous to the type II secretion pathway.","authors":"R Riekki, T Palomäki, O Virtaharju, H Kokko, M Romantschuk, H T Saarilahti","doi":"10.1007/pl00008691","DOIUrl":"https://doi.org/10.1007/pl00008691","url":null,"abstract":"A cellulase-producing clone was isolated from a genomic library of the Erwinia rhapontici (Millard) Burkholder strain NCPPB2989. The corresponding gene, named celA, encodes an endoglucanase (EC 3.2.1.4) with the extremely low pH optimum of 3.4 and a temperature optimum between 40 and 50 degrees C. A single ORF of 999 nt was found to be responsible for the Cel activity. The corresponding protein, named CelA, showed 67% identity to the endoglucanase Y of E. chrysanthemi and 51.5% identity to the endoglucanase of Cellulomonas uda, and thus belongs to the glycosyl hydrolase family 8. The celA gene, or its homologue, was found to be present in all E. rhapontici isolates analysed, in E. chrysanthemi, and in E. amylovora. The presence of plant cell wall-degrading enzymes in the amylovora group of Erwinia spp. had not previously been established. Furthermore, the DNA of both E. rhapontici and E. amylovora was found to exhibit homology to genes encoding the type II (GSP) secretion pathway, which is known to be responsible for extracellular targeting of cellulases and pectinases in Erwinia spp. that cause soft rotting, such as E. carotovora and E. chrysanthemi. Secretion of the CelA protein by E. rhapontici could not be verified. However, the CelA protein itself was found to include the information necessary for heterologous secretion by E. chrysanthemi.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"263 6","pages":"1031-7"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/pl00008691","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21790920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Molecular characterisation of a new mutant allele of the plastid phosphoglucomutase in Arabidopsis, and complementation of the mutant with the wild-type cDNA. 拟南芥质体磷酸葡萄糖糖化酶一个新突变等位基因的分子特征及其与野生型cDNA的互补。

Molecular & general genetics : MGG Pub Date : 2000-07-01 DOI: 10.1007/pl00008698

H Kofler, R E Häusler, B Schulz, F Gröner, U I Flügge, A Weber

{"title":"Molecular characterisation of a new mutant allele of the plastid phosphoglucomutase in Arabidopsis, and complementation of the mutant with the wild-type cDNA.","authors":"H Kofler, R E Häusler, B Schulz, F Gröner, U I Flügge, A Weber","doi":"10.1007/pl00008698","DOIUrl":"https://doi.org/10.1007/pl00008698","url":null,"abstract":"Screening of transposon-associated mutants of Arabidopsis thaliana for altered starch metabolism resulted in the isolation of a mutant that did not accumulate starch in any tissue or at any developmental stage (starch-free mutant, stf1). Allelism tests with known mutants showed that stf1 represents a new mutant allele of the plastid isoform of the enzyme phosphoglucomutase (PGMp). The mutation was mapped to chromosome 5. An Arabidopsis EST that showed significant homology to the cytosolic isoform of phosphoglucomutase (PGM) from maize was able to complement the mutant phenotype. The Arabidopsis EST was transcribed and translated in vitro and the protein product was efficiently imported into isolated chloroplasts and processed to its mature form. The lack of starch biosynthesis in stf1 is accompanied by the accumulation of soluble sugars. The rate of CO2 assimilation measured in individual leaves was substantially diminished only under conditions of high CO2 and low O2. Remarkably, stf1 exhibits an increase rather than a decrease in total leaf PGM activity, suggesting an induction of the cytosolic isoform(s) in the mutant. The substrate for PGM, glucose 6-phosphate, accumulated in stf1 during the day, resulting in 10-fold higher content than in the wild type at the end of the photoperiod.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"263 6","pages":"978-86"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/pl00008698","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21790914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 73

Distinct requirements for the AP-3 adaptor complex in pigment granule and synaptic vesicle biogenesis in Drosophila melanogaster. 黑胃果蝇色素颗粒和突触囊泡生物形成对AP-3接头复合物的不同要求。

Molecular & general genetics : MGG Pub Date : 2000-07-01 DOI: 10.1007/pl00008688

C Mullins, L M Hartnell, J S Bonifacino

{"title":"Distinct requirements for the AP-3 adaptor complex in pigment granule and synaptic vesicle biogenesis in Drosophila melanogaster.","authors":"C Mullins, L M Hartnell, J S Bonifacino","doi":"10.1007/pl00008688","DOIUrl":"https://doi.org/10.1007/pl00008688","url":null,"abstract":"The AP-3 adaptor protein complex has been implicated in the biogenesis of lysosome-related organelles, such as pigment granules/melanosomes, and synaptic vesicles. Here we compare the relative importance of AP-3 in the biogenesis of these organelles in Drosophila melanogaster. We report that the Drosophila pigmentation mutants orange and ruby carry genetic lesions in the sigma3 and beta3-adaptin subunits of the AP-3 complex, respectively. Electron microscopy reveals dramatic reductions in the numbers of electron-dense pigment granules in the eyes of these AP-3 mutants. Mutant flies also display greatly reduced levels of pigments housed in these granules. In contrast, electron microscopy of retinula cells reveals numerous synaptic vesicles in both AP-3 mutant and wild-type flies, while behavioral assays show apparently normal locomotor ability of AP-3 mutant larvae. Together, these results demonstrate that Drosophila AP-3 is critical for the biogenesis of pigment granules, but is apparently not essential for formation of a major population of synaptic vesicles in vivo.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"263 6","pages":"1003-14"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/pl00008688","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21790917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 61

Identification of a hybrid-specific expressed gene encoding novel RNA-binding protein in wheat seedling leaves using differential display of mRNA. 利用mRNA的差异显示鉴定小麦幼苗叶片中一个新的rna结合蛋白的杂交特异性表达基因。

Molecular & general genetics : MGG Pub Date : 2000-07-01 DOI: 10.1007/pl00008693

Z Ni, Q Sun, Z Liu, L Wu, X Wang

{"title":"Identification of a hybrid-specific expressed gene encoding novel RNA-binding protein in wheat seedling leaves using differential display of mRNA.","authors":"Z Ni, Q Sun, Z Liu, L Wu, X Wang","doi":"10.1007/pl00008693","DOIUrl":"https://doi.org/10.1007/pl00008693","url":null,"abstract":"A hybrid-specific expressed cDNA fragment, designated as AG5, has been identified in wheat seedling leaves using differential mRNA display. AG5 contains an open reading frame (ORF) encoding 183 amino acid residues. Comparison with amino acid sequences in GenBank revealed that the AG5 protein is homologous to a group of Gly-rich proteins with consensus sequence-type RNA-binding domains (CS-RBD). Structural analysis showed that AG5 protein contains five motifs, including a consensus sequence-type RNA-binding domain near its N-terminus, arginine/aspartic acid repeats and a Gly-rich region in its center, a Cys-X2-Cys-X4-His-X4-Cys (CCHC) zinc finger motif in the Gly-rich region, and TrySer2ArgAsp2Arg repeats towards its C-terminus. Of all previously described RNA-binding proteins, only RZ-1 from tobacco has a similar structure to the AG5 protein, but RZ-1 lacks a TrySer2ArgAsp2Arg repeat motif, indicating that the two proteins may belong to a family of closely related proteins in plants. The possible role of AG5 and its relation to wheat heterosis are discussed.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"263 6","pages":"934-8"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/pl00008693","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21791640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Genetic analysis of the signal-sensing region of the histidine protein kinase VirA of Agrobacterium tumefaciens. 肿瘤农杆菌组氨酸蛋白激酶 VirA 信号感应区的遗传分析。

Molecular & general genetics : MGG Pub Date : 2000-07-01 DOI: 10.1007/pl00008694

A Toyoda-Yamamoto, N Shimoda, Y Machida

{"title":"Genetic analysis of the signal-sensing region of the histidine protein kinase VirA of Agrobacterium tumefaciens.","authors":"A Toyoda-Yamamoto, N Shimoda, Y Machida","doi":"10.1007/pl00008694","DOIUrl":"10.1007/pl00008694","url":null,"abstract":"The membrane-bound sensor protein kinase VirA of Agrobacterium tumefaciens detects plant phenolic substances, which induce expression of vir genes that are essential for the formation of the crown gall tumor. VirA also responds to specific monosaccharides, which enhance vir expression. These sugars are sensed by the periplasmic domain of VirA that includes the region homologous to the chemoreceptor Trg, and the phenolics are thought to be detected by a part of the cytoplasmic linker domain, while the second transmembrane domain (TM2) is reported to be nonessential. To define regions of VirA that are essential for signal sensing, we introduced base-substitution and deletion mutations into coding regions that are conserved among the respective domains of VirA proteins from various Agrobacterium strains, and examined the effects of these mutations on vir induction and tumorigenicity. The results show that the Trg-homologous region in the periplasmic domain is not essential for the enhancement of vir gene expression by sugars. Most mutations in the TM2 domain also failed to influence enhancement by sugars and reduced the level of vir induction, but a mutation in the TM2 region adjacent to the cytoplasmic linker abolished induction of the vir genes. In the linker domain, sites essential for vir induction by phenolics were scattered over the entire region. We propose that a topological feature formed by the linker domain and at least part of the TM2 may be crucial for activation of a membrane-anchored VirA protein. Complementation analysis with two different VirA mutants suggested that intermolecular phosphorylation between VirA molecules occurs in vivo, and that two intact periplasmic regions in a VirA dimer are required for the enhancement of vir induction by sugars.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"263 6","pages":"939-47"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/pl00008694","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21791641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26