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Flow cytometric analysis of a marine LAS-degrading consortia. 海洋las降解菌群的流式细胞分析。
Microbios Pub Date : 2000-01-01
R López-Amorós, J Comas, M T Garcia, J Vives-Rego
{"title":"Flow cytometric analysis of a marine LAS-degrading consortia.","authors":"R López-Amorós,&nbsp;J Comas,&nbsp;M T Garcia,&nbsp;J Vives-Rego","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The specific nucleic acid fluorochrome SYTO-13 was used in flow cytometric analysis to assess changes in the density and heterogeneity of marine bacterial populations which biodegrade linear alkylbenzene sulphonate (LAS). Seawater samples with LAS and incubated in the laboratory (20 degrees C, 100 rpm, 30 days) were used to monitor LAS-degrading consortia. Flow cytometric studies and culture methods were used to characterize the LAS degrading bacterioplankton consortia. Fluorescence and scatter signals enabled us to define three regions (R1, R2 and R3) in the dual parameter cytograms. The distribution of the bacterial counts in these regions allowed us to monitor the formation and evolution of the consortia.</p>","PeriodicalId":18494,"journal":{"name":"Microbios","volume":"101 398","pages":"23-36"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21531874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of media composition of delta-endotoxin production and morphology of Bacillus thuringiensis in wild types and spontaneously mutated strains. 苏云金芽孢杆菌野生型和自发突变菌株培养基组成对δ内毒素产生和形态的影响。
Microbios Pub Date : 2000-01-01
M Perani, A H Bishop
{"title":"Effects of media composition of delta-endotoxin production and morphology of Bacillus thuringiensis in wild types and spontaneously mutated strains.","authors":"M Perani,&nbsp;A H Bishop","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bacillus thuringiensis strains HD-73 and 4412, and two spontaneous mutants termed 4412aa-ind and 4412sph-cry were studied for the ability to produce crystals of different size and shape when grown in a rich medium and in an appropriate minimal medium defined during this study. Strain 4412aa-ind showed medium-dependent variation in the crystal phenotype. Scanning electron microscopy was utilized in order to show crystal variations in size and shape. B. thuringiensis strains 4412aa-ind and 4412sph-cry grown in rich and in minimal media produced differences in crystal morphology, and SDS-PAGE gel indicated that crystal variation was only at the morphological level and not in composition of the amino acids. A further nineteen B. thuringiensis strains were tested for the ability to sporulate in two simple defined media. Of these strains thirteen were able to complete sporulation with crystal production in one or both media. All strains grew and sporulated in a medium containing the usual twenty amino acids, and no vitamins or other growth factors were required.</p>","PeriodicalId":18494,"journal":{"name":"Microbios","volume":"101 398","pages":"47-66"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21531876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new factor from Bacillus mesentericus which promotes the growth of Bifidobacterium. 从肠系膜芽孢杆菌中提取的一种促进双歧杆菌生长的新因子。
Microbios Pub Date : 2000-01-01
G Seo, Y Akimoto, H Hamashima, K Masuda, K Shiojima, C Sakuma, M Sasatsu, T Arai
{"title":"A new factor from Bacillus mesentericus which promotes the growth of Bifidobacterium.","authors":"G Seo,&nbsp;Y Akimoto,&nbsp;H Hamashima,&nbsp;K Masuda,&nbsp;K Shiojima,&nbsp;C Sakuma,&nbsp;M Sasatsu,&nbsp;T Arai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It was reported previously that supernatants of cultures of Bacillus mesentericus TO-A promote the growth of Bifidobacterium species. In this study, a new growth-promoting factor, BM-1, was purified from the supernatant of such a culture and its chemical structure was determined. BM-1 was identified as 3,3-dihydroxyazetidine, and it promoted the growth of several strains of Bifidobacterium.</p>","PeriodicalId":18494,"journal":{"name":"Microbios","volume":"101 399","pages":"105-14"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21589506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Fusobacterium necrophorum subspecies necrophorum on extracellular matrix of tissue-cultured bovine kidney cells. 坏死性梭杆菌亚种坏死性梭杆菌对组织培养牛肾细胞细胞外基质的影响。
Microbios Pub Date : 2000-01-01
Y Okada, M Kanoe, K Okamoto, K Sakamoto, Y Yaguchi, T Watanabe
{"title":"Effects of Fusobacterium necrophorum subspecies necrophorum on extracellular matrix of tissue-cultured bovine kidney cells.","authors":"Y Okada,&nbsp;M Kanoe,&nbsp;K Okamoto,&nbsp;K Sakamoto,&nbsp;Y Yaguchi,&nbsp;T Watanabe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effects of Fusobacterium necrophorum subsp. necrophorum on the extracellular matrix were investigated. The toxic preparation from the culture induced reduction in the number of tissue-cultured bovine kidney cells. The exposed cells often manifested partial loss of cytoplasm and were morphologically irregular. Scanning electron microscopy demonstrated partial loss of the microvilli on the exposed cells and roughness of the cell surfaces. Finally, sodium dodecyl sulphate polyacrylamide gel electrophoresis profiles revealed complete degradation of bovine collagen type 1 after treatment with the toxic preparation. This degradation was inhibited by the addition of homologous antiserum. These findings indicate that the degradation may contribute to the establishment of the infection caused by F.n. subsp. necrophorum.</p>","PeriodicalId":18494,"journal":{"name":"Microbios","volume":"101 400","pages":"147-56"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21606914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The spectrum of pathogenic bacteria in positive blood cultures. 阳性血培养中致病菌的光谱。
Microbios Pub Date : 2000-01-01
M A Bahar, R T Kilani, A Ghahary
{"title":"The spectrum of pathogenic bacteria in positive blood cultures.","authors":"M A Bahar,&nbsp;R T Kilani,&nbsp;A Ghahary","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>There is compelling evidence to suggest that the profiles of pathogenic bacteria which cause septicaemia shock vary from one region to another due to differences in the source of contamination. Blood cultures were prepared from 3,481 patients with symptoms of systemic bacterial contamination. The blood cultures of 558 (16.02%) patients showed at least one kind of bacterial infection. This rate was markedly higher than that reported in Germany (12.8%) and Japan (12.3%). Systemic bacterial infection was significantly higher in males than in females (82% versus 18%). Most of the patients surveyed (62%) were adults and the rest were either infants (19%) or neonates (19%). When blood samples of these patients were cultured, and isolated bacteria were characterized by a variety of diagnostic tests, over twenty different strains of bacteria were identified and characterized. More than 29% of positive cultures were Enterobacter spp. while Staphylococcus aureus (20%) and Brucella spp. (8%) ranked second and third highest among the infections. The results suggest that agents which cause infections vary with respect to region and that knowledge of local risk factors may aid in patient diagnosis and treatment.</p>","PeriodicalId":18494,"journal":{"name":"Microbios","volume":"103 405","pages":"107-17"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21917468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hepatitis C virus and Epstein-Barr virus: dual infection or atypical antibody response. 丙型肝炎病毒和eb病毒:双重感染或非典型抗体反应。
Microbios Pub Date : 2000-01-01
J Gutiérrez, M Rodríguez-Iglesias
{"title":"Hepatitis C virus and Epstein-Barr virus: dual infection or atypical antibody response.","authors":"J Gutiérrez,&nbsp;M Rodríguez-Iglesias","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Positive serological reactions against hepatitis C virus (HCV) appeared in the course of Epstein-Barr virus (EBV) infectious mononucleosis. In 429 consecutive patients with high levels of transaminases, 28 patients with EBV primary infection were found. The presence of anti-HCV antibodies and HCV RNA was studied in these subjects. In seven patients anti-HCV antibodies (C33 and C22c RIBA bands) were detected, but all were polymerase chain reaction (PCR) negative. These results may have been due solely to a HCV infection or were an atypical response to HCV in the course of infectious mononucleosis.</p>","PeriodicalId":18494,"journal":{"name":"Microbios","volume":"103 404","pages":"65-6"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21864678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of HCV and GBV-CHGV RNA in peripheral blood mononuclear cells of patients with chronic type C hepatitis. 慢性丙型肝炎患者外周血单个核细胞HCV及GBV-CHGV RNA的检测
Microbios Pub Date : 2000-01-01
F García, F García, C Roldán, I López, N M Martínez, M Alvarez, M C Bernal, J Hernández, M C Maroto
{"title":"Detection of HCV and GBV-CHGV RNA in peripheral blood mononuclear cells of patients with chronic type C hepatitis.","authors":"F García,&nbsp;F García,&nbsp;C Roldán,&nbsp;I López,&nbsp;N M Martínez,&nbsp;M Alvarez,&nbsp;M C Bernal,&nbsp;J Hernández,&nbsp;M C Maroto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hepatitis C virus (HCV) and hepatitis G virus (HGV) viraemia were investigated by RT-PCR protocols in peripheral blood mononuclear cells (PBMC) of 22 patients with chronic type C hepatitis. Samplings were at basal and 4-8 months after a 12 month period of treatment with interferon-alpha. A plus strand of HCV in PBMC was detected in 8 of 21 patients (38%) (p <0.05; chi2 test) with a lack of response to therapy; a minus strand was detected in 10% of chronic type C hepatitis and 25% of the patients harboured HCV RNA in PBMC. The association with a response was nearly significant (p <0.1; chi2 test). GBV-C/HGV RNA was detected in the serum of 9 of 21 (43%) patients and in PBMC of 20% of the patients viraemic. Genomic sequences of GBVC/HGV in PBMC were found, but further investigation is needed to assess the findings reported for HCV.</p>","PeriodicalId":18494,"journal":{"name":"Microbios","volume":"103 404","pages":"7-15"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21864775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plasmid-mediated catabolism of dicamba by Pseudomonas species strain PXM. PXM假单胞菌质粒介导麦草畏的分解代谢。
Microbios Pub Date : 2000-01-01
A Khalil, D J Cork
{"title":"Plasmid-mediated catabolism of dicamba by Pseudomonas species strain PXM.","authors":"A Khalil,&nbsp;D J Cork","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pseudomonas species strain PXM, which is able to use dicamba (3,6-dichloro-2-methoxybenzoic acid; CAS 1918-00-9, Banvel) as its sole carbon source for growth, has been isolated. The catabolism of dicamba and some of its putative metabolic descendants correlates with the presence of a large and unstable plasmid.</p>","PeriodicalId":18494,"journal":{"name":"Microbios","volume":"102 403","pages":"183-91"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21792833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Distribution of diaminopropane, putrescine and cadaverine in Haemophilus and Actinobacillus. 二氨基丙烷、腐胺和尸胺在嗜血杆菌和放线菌中的分布。
Microbios Pub Date : 2000-01-01
K Hamana, K Nakata
{"title":"Distribution of diaminopropane, putrescine and cadaverine in Haemophilus and Actinobacillus.","authors":"K Hamana,&nbsp;K Nakata","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cellular levels of diaminopropane, putrescine and cadaverine, and decarboxylase activities to produce these diamines in six species (16 strains) of Haemophilus and four species (5 strains) of Actinobacillus belonging to the family Pasteurellaceae of the gamma subclass of the class Proteobacteria, were determined by high performance liquid chromatography (HPLC). Diaminopropane was ubiquitously distributed within all Haemophilus and Actinobacillus species, and L-2,4-diaminobutyric acid decarboxylase activity was detected in them. Putrescine and ornithine decarboxylase activity were found in H. aphrophilus, H. parainfluenzae and H. influenzae (type a, b, d, e and f except for type c) but not detected in H. aegyptius, H. parahaemolyticus, H. ducreyi and Actinobacillus species. Cadaverine occurred in H. aphrophilus, H. aegyptius, H. influenzae, H. parainfluenzae, A. actinomycetemcomitans, A. equuli and A. lignieresii, whereas their lysine decarboxylase activity was scarcely detected. Cadaverine was not found in H. parahaemolyticus, H. ducreyi and A. suis. The diamine profile serves as a phenotypic marker for the chemotaxonomic classification of the family Pasteurellaceae.</p>","PeriodicalId":18494,"journal":{"name":"Microbios","volume":"103 404","pages":"43-51"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21864675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Homogeneity of Trypanosoma evansi isolates from domestic and sylvatic mammals from the Pantanal of Mato Grosso. 马托格罗索州潘塔纳尔地区家养和森林哺乳动物伊瓦西锥虫分离株的同质性。
Microbios Pub Date : 2000-01-01
A O Quieroz, N S Nehme-Russell, A Brandão, A M Jansen
{"title":"Homogeneity of Trypanosoma evansi isolates from domestic and sylvatic mammals from the Pantanal of Mato Grosso.","authors":"A O Quieroz,&nbsp;N S Nehme-Russell,&nbsp;A Brandão,&nbsp;A M Jansen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>'Mal de Cadeiras' is a disease which causes great mortality in horses in the Pantanal Matogrossense region, Brazil. The agent of this disease is Trypanosoma evansi, a kinetoplastid flagellate which belongs to the Trypanosomatidae family, classified into the Salivarian section. Transmission occurs mechanically by haematophagous Diptera, mainly by Stomoxys sp. and Tabanus sp. and vampire bats. Outbreaks of Mal de Cadeiras in horses result in economic losses, thus limiting their use in cattle raising. Ten isolates of T. evansi recently derived from coati (Nasua nasua, Carnivora, Procyonidae), horses and dogs were compared, using schizodeme analyses from DNA digested by the restriction enzyme Hin fl. The results showed similar electrophoretic profiles for all isolates from wherever the host came. Homogeneity of isolates from domestic and sylvatic animals suggested two hypotheses: (1) the parasites circulated in only one transmission cycle;, and (2) independent cycles were not established in sufficient time to modify the molecular profiles of the isolates.</p>","PeriodicalId":18494,"journal":{"name":"Microbios","volume":"103 404","pages":"27-30"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21864777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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