Medical Science Monitor Basic Research最新文献_第9页

Spalt-Like Transcription Factor 1 (SALL1) Gene Expression Inhibits Cell Proliferation and Cell Migration of Human Glioma Cells Through the Wnt/β-Catenin Signaling Pathway. Spalt-Like转录因子1 (SALL1)基因表达通过Wnt/β-Catenin信号通路抑制胶质瘤细胞增殖和细胞迁移

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2019-05-01 DOI: 10.12659/MSMBR.915067

Dapeng Chi, Wei Zhang, Yulong Jia, Damin Cong, Shaoshan Hu

{"title":"Spalt-Like Transcription Factor 1 (SALL1) Gene Expression Inhibits Cell Proliferation and Cell Migration of Human Glioma Cells Through the Wnt/β-Catenin Signaling Pathway.","authors":"Dapeng Chi, Wei Zhang, Yulong Jia, Damin Cong, Shaoshan Hu","doi":"10.12659/MSMBR.915067","DOIUrl":"https://doi.org/10.12659/MSMBR.915067","url":null,"abstract":"BACKGROUND The spalt-like transcription factor 1 (SALL1) gene is a member of the Krüppel-associated box-containing zinc finger proteins (KRAB-ZFPs) and has been shown to modulate the onset and progression of human tumors. This study aimed to investigate the regulatory effects and mechanisms of SALL1 gene expression in human glioblastoma and glioma cells and tissue samples from patients with cerebral glioma. MATERIAL AND METHODS The human glioblastoma cell lines, LN229, U87-MG, U-251, U343, and the Hs683 glioma cell line were studied. The cell counting kit-8 (CCK-8) assay, cell cycle assay, wound-healing assay, transwell assay, Western blot, and quantitative real-time polymerase chain reaction (qRT-PCR) were used to evaluate cell proliferation, cell migration, and the cell cycle and expression of SALL1. Expression of SALL1 mRNA in 120 samples of cerebral glioma and 20 samples of normal brain were studied. Overall survival data from patients with cerebral glioma were analyzed. RESULTS SALL1 expression was down-regulated in human glioblastoma and glioma cells and in cerebral glioma tissues. Down-regulation of SALL1 expression was associated with reduced overall survival. Overexpression of SALL1 was associated with inhibition of cell proliferation associated with cell cycle arrest at the G0/G1 phase. SALL1 inhibited cell migration by preventing epithelial-mesenchymal transition (EMT) and down-regulating the expression of stem cell markers. Reduced levels of ß-catenin and downregulation of c-Myc and cyclin D1 and upregulation of p21and p27 expression were associated with SALL1 expression. CONCLUSIONS In human glioblastoma cells and cerebral glioma tissues, SALL1 acted as a tumor suppressor gene by inhibiting Wnt/ß-catenin signaling.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/da/5c/medscimonitbasicres-25-128.PMC6511114.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37197592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Changes in Shape and Size Discrimination and State Anxiety After Alternate-Nostril Yoga Breathing and Breath Awareness in One Session Each. 交替鼻孔瑜伽呼吸和呼吸意识后的形状和大小辨别和状态焦虑的变化。

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2019-04-22 DOI: 10.12659/MSMBR.914956

Shirley Telles, Babita Vishwakarma, Ram Kumar Gupta, Acharya Balkrishna

{"title":"Changes in Shape and Size Discrimination and State Anxiety After Alternate-Nostril Yoga Breathing and Breath Awareness in One Session Each.","authors":"Shirley Telles, Babita Vishwakarma, Ram Kumar Gupta, Acharya Balkrishna","doi":"10.12659/MSMBR.914956","DOIUrl":"https://doi.org/10.12659/MSMBR.914956","url":null,"abstract":"BACKGROUND Yoga breathing techniques like high-frequency yoga breathing (HFYB) and breath awareness (BAW) have been associated with improved performance in the shape and size discrimination task. A PubMed search of the literature revealed that alternate-nostril breathing has been shown to improve performance in attention tasks, but the effect on tactile perception has not been studied. Hence, the present study was designed to assess the immediate effects of alternate-nostril yoga breathing (ANYB) compared to breath awareness on shape and size discrimination and state anxiety. MATERIAL AND METHODS Fifty healthy male volunteers ages 20-50 years (group mean ±S.D., 28.4±8.2 years) were recruited. Each participant was assessed in 3 sessions conducted on 3 separate days at the same time of day. The 3 sessions were (i) alternate-nostril yoga breathing (ANYB), (ii) breath awareness (BAW), and (iii) quiet sitting (QS), and the sequence of the sessions was randomly allocated. The shape and size discrimination task and state anxiety were assessed before and after all 3 sessions. Repeated measures analysis of variance (RM-ANOVA) followed by post hoc tests for multiple comparisons, which were Bonferroni-adjusted, were performed to compare data before and after all 3 sessions using SPSS version 18.0. RESULTS The errors scores in the shape and size discrimination task showed a significant reduction after the ANYB session (p<0.001). A significant reduction was found in the level of state anxiety after breath awareness (p<0.05) and quiet sitting sessions (p<0.001). CONCLUSIONS The present results suggest that ANYB: (i) improves performance in a task which requires perceptual sensitivity and focused attention, but (ii) does not reduce state anxiety following this task.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2019-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/27/73/medscimonitbasicres-25-121.PMC6496972.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37334232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Polyethylenimine (PEI)-Mediated E1A Increases the Sensitivity of Hepatocellular Carcinoma Cells to Chemotherapy. 聚乙烯亚胺(PEI)介导的E1A增加肝癌细胞对化疗的敏感性

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2019-04-08 DOI: 10.12659/MSMBR.914811

Zhanfeng Li, Zhifeng Yao, Yiwen Zhang, Jianxin Yao, Zhiyao Pan, Jinfei Chen

{"title":"Polyethylenimine (PEI)-Mediated E1A Increases the Sensitivity of Hepatocellular Carcinoma Cells to Chemotherapy.","authors":"Zhanfeng Li, Zhifeng Yao, Yiwen Zhang, Jianxin Yao, Zhiyao Pan, Jinfei Chen","doi":"10.12659/MSMBR.914811","DOIUrl":"https://doi.org/10.12659/MSMBR.914811","url":null,"abstract":"BACKGROUND The aim of this study was to assess the ability of polyethylenimine (PEI) as an E1A plasmid vector to transfect hepatocellular carcinoma SMMC-7721 cells and to analyze the sensitization effect of E1A on various anti-tumor drugs. MATERIAL AND METHODS PEI-mediated recombinant plasmid psv-E1A with high expression of the E1A gene was introduced into hepatocellular carcinoma SMMC-7721 cells, and the effective transfection of E1A gene was determined by RT-PCR and Western blot analysis. The CCK8 method was used to detect the proliferation inhibition of docetaxel, epirubicin, gemcitabine, and 5-fluorouracil on SMMC-7721 cells before and after the transfection of the E1A gene. RESULTS RT-PCR and Western blot analysis showed that PEI could transfect plasmid psv-E1A with stable expression. After the transfection of E1A gene, the sensitivity of SMMC-7721 cells to docetaxel, epirubicin, gemcitabine, and 5-fluorouracil was increased (P<0.05), and the sensitivity to docetaxel was significantly improved (P<0.01). CONCLUSIONS PEI can transfect plasmid psv-E1A. The E1A gene can increase the sensitivity of hepatocellular carcinoma cells to chemotherapeutic drugs. The mechanism may be related to the increased ability of the E1A gene to inhibit proliferation of hepatocellular carcinoma cells and altering the cell cycle of hepatocellular carcinoma cells.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2019-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/15/5a/medscimonitbasicres-25-113.PMC6475126.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37127128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VEGFR-2 Is in a State of Activation in Hair Follicles, Sebaceous Glands, Eccrine Sweat Glands, and Epidermis from Human Scalp: An In Situ Immunohistochemistry Study of Phosphorylated VEGFR-2. 人体头皮的毛囊、皮脂腺、汗腺和表皮中VEGFR-2处于激活状态:磷酸化VEGFR-2的原位免疫组织化学研究

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2019-03-28 DOI: 10.12659/MSMBR.914570

Xian-Jie Wu, Jing Jing, Zhong-Fa Lu, Min Zheng

{"title":"VEGFR-2 Is in a State of Activation in Hair Follicles, Sebaceous Glands, Eccrine Sweat Glands, and Epidermis from Human Scalp: An In Situ Immunohistochemistry Study of Phosphorylated VEGFR-2.","authors":"Xian-Jie Wu, Jing Jing, Zhong-Fa Lu, Min Zheng","doi":"10.12659/MSMBR.914570","DOIUrl":"https://doi.org/10.12659/MSMBR.914570","url":null,"abstract":"BACKGROUND Recent research reports that VEGFR-2 is expressed in the whole hair follicle, sebaceous glands, eccrine sweat glands, and epidermis. However, phosphorylated VEGFR-2 was not found, and it could not be ascertained whether the activated form of VEGFR-2 actually participates in the biological control of epidermal appendages. In this study we aimed to determine whether the VEGFR-2 pathway is directly involved in the daily regulation of epidermal appendages biology. MATERIAL AND METHODS In this study, we investigated the expression of phosphorylation of VEGFR-2 by immunohistochemical analysis in the epidermis and epidermal appendages in normal human scalp skin. RESULTS Immunohistochemical analysis revealed phosphorylation of VEGFR-2 in a whole hair follicle, mainly in the infundibulum basal layer, hair cortex, and medulla in the isthmus, and matrix in the hair bulb. Phosphorylated VEGFR-2 also was found in the sebaceous glands, eccrine sweat glands, and epidermis. CONCLUSIONS Therefore, we suggest that VEGFR-2 activation is involved in routine regulation of human epidermal appendages.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2019-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.12659/MSMBR.914570","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37096335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effect of Serum Deprivation Stress on Signal Induction Regulatory Protein-Alpha (SIRP-Alpha)-Mediated Erythrophagocytosis by Macrophages. 血清剥夺应激对信号诱导调节蛋白α（SIRPα）介导的巨噬细胞红吞噬作用的影响。

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2019-03-21 DOI: 10.12659/MSMBR.912946

Zakaria Hindi, AbdAllah Gad, Courtney Jarvis, Talal Zahoor, Craig Spellman, Stephanie Filleur

{"title":"Effect of Serum Deprivation Stress on Signal Induction Regulatory Protein-Alpha (SIRP-Alpha)-Mediated Erythrophagocytosis by Macrophages.","authors":"Zakaria Hindi, AbdAllah Gad, Courtney Jarvis, Talal Zahoor, Craig Spellman, Stephanie Filleur","doi":"10.12659/MSMBR.912946","DOIUrl":"10.12659/MSMBR.912946","url":null,"abstract":"BACKGROUND Hemophagocytic lymphohistiocytosis (HLH) is a rare syndrome that involves loss of macrophages' self-cells recognition resulting in auto-phagocytosis of erythrocytes, leukocytes, and platelets and leading to multi-system effects. The pathogenesis of HLH is unclear but can be explained by malfunction of the physiologic inhibitory pathway through interaction between macrophage SIRP-alpha and erythrocyte CD 47. The goal of the present study was to evaluate if erythrocytes phagocytosis occurs as a result of altered macrophage SIRP-alpha expression during inflammatory/stressful conditions as seen in HLH. MATERIAL AND METHODS RAW264.7 macrophages were cultured in serum-free media (SFM) and complete media (CM) to simulate stressful and physiologic conditions, respectively. CD47+ mouse erythrocytes were used to test interactions with macrophages at different stages. SIRP-alpha expressions and phagocytosis assays were measured and analyzed at different steps. The study was in vitro and used murine cells to simulate in vivo human interactions. RESULTS SIRP-alpha expressions and phagocytosis rates were higher in SFM compared to CM. Interestingly, after adding SIRP-alpha blocking antibodies (Ab), phagocytosis rates significantly decreased. CONCLUSIONS Serum deprivation and LPS/INF-Gamma induction resulted in increased SIRP-alpha expression and erythrophagocytosis. Using SIRP-alpha Ab during this condition decreased the rate of erythrophagocytosis, which indicates that SIRP-alpha receptor can have pro-phagocytic activity.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2019-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/af/bf/medscimonitbasicres-25-100.PMC6441304.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37075866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Composition and Biological Activities of Lebanese Pentapera Plant. 黎巴嫩五芒菜属植物的化学成分及生物活性。

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2019-03-14 DOI: 10.12659/MSMBR.914741

Hany Kallassy, Hussein Fayyad-Kazan, Rawan Makki, Monya Kaeen, Amer Sakr, Osama Alyamani, Rim El Dirani, Eva Hamade, Mohammad Fayyad-Kazan, Bassam Badran

{"title":"Chemical Composition and Biological Activities of Lebanese Pentapera Plant.","authors":"Hany Kallassy, Hussein Fayyad-Kazan, Rawan Makki, Monya Kaeen, Amer Sakr, Osama Alyamani, Rim El Dirani, Eva Hamade, Mohammad Fayyad-Kazan, Bassam Badran","doi":"10.12659/MSMBR.914741","DOIUrl":"https://doi.org/10.12659/MSMBR.914741","url":null,"abstract":"BACKGROUND Due to their chemical constituents and biological properties, plants have long been used to control life-threatening diseases. The flora of Lebanon includes many plants that have already been demonstrated to have medicinal value, and other species, such as Pentapera sicula libanotica, that are yet to be characterized. The present study characterized the chemical composition, anti-oxidant, anti-inflammatory, and anti-proliferative potential of aqueous, ethanol, and methanol extracts derived from the leaves of the Lebanese Pentapera plant. MATERIAL AND METHODS High-performance liquid chromatography (HPLC) was used to determine the chemical composition. Gas chromatography (GC) coupled with mass spectrometry (MS) was applied to determine the content of essential oil. DPPH radical scavenging assay was performed to evaluate the anti-oxidant potential. The anti-inflammatory potential was assessed using quantitative real-time PCR (qRT-PCR) by measuring TNF-alpha, IL-6, and CCL4 mRNA levels, and we assessed Cox-2 and iNOS proteins levels using Western blot (WB) analysis. MTT assay was carried out to determine the anti-proliferative potential. RESULTS We identified, mainly in the alcoholic (methanol and ethanol) extracts, distinct bioactive compounds with pharmacological relevance. In parallel, with their phytochemical content, these 2 extracts showed significant anti-oxidant, anti-inflammatory and anti-proliferative capacities. CONCLUSIONS Pentapera sicula libanotica appears to be a promising pharmacological tool.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2019-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.12659/MSMBR.914741","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37228105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

New Insights of Uterine Leiomyoma Pathogenesis: Endocannabinoid System. 子宫平滑肌瘤发病机制的新认识:内源性大麻素系统。

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2019-03-07 DOI: 10.12659/MSMBR.914019

Thangesweran Ayakannu, Anthony H Taylor, Timothy H Marczylo, Justin C Konje

{"title":"New Insights of Uterine Leiomyoma Pathogenesis: Endocannabinoid System.","authors":"Thangesweran Ayakannu, Anthony H Taylor, Timothy H Marczylo, Justin C Konje","doi":"10.12659/MSMBR.914019","DOIUrl":"https://doi.org/10.12659/MSMBR.914019","url":null,"abstract":"BACKGROUND The aim of this study was to determine if components of the endocannabinoid system are modulated in uterine leiomyomas (fibroids). Components studied included cannabinoid receptors 1 (CB1) and 2 (CB2); the G protein-coupled receptor GPR55; transient potential vanilloid receptor 1 (TRPV1) and the endocannabinoid modulating enzymes N-acylphosphatidylethanolamine-specific phospholipase D (NAPE-PLD) and fatty acid amide hydrolase (FAAH), and their N-acylethanolamine (NAE) ligands: N-arachidonylethanolamine (AEA), N-oleoylethanolamine (OEA), and N-palmityolethanaolamine (PEA). MATERIAL AND METHODS Transcript levels of CB1, CB2, TRPV1, GPR55, NAPE-PLD, and FAAH were measured using RT-PCR and correlated with the tissue levels of the 3 NAEs in myometrial tissues. The tissues studied were: 1) fibroids, 2) myometrium adjacent/juxtaposed to the fibroid lesions, and 3) normal myometrium. Thirty-seven samples were processed for NAE measurements and 28 samples were used for RT-PCR analyses. RESULTS FAAH expression was significantly lower in fibroids, resulting in a NAPE-PLD: FAAH ratio that favors higher AEA levels in pre-menopausal tissues, whilst PEA levels were significantly lower, particularly in post-menopausal women, suggesting PEA protects against fibroid pathogenesis. The CB1: CB2 ratio was lower in fibroids, suggesting that loss of CB1 expression affects the fibroid cell phenotype. Significant correlations between reduced FAAH, CB1, and GPR55 expression and PEA in fibroids indicate that the loss of these endocannabinoid system components are biomarkers of leiomyomata. CONCLUSIONS Loss of expression of CB1, FAAH, GPR55, and PEA production are linked to the pathogenesis of uterine fibroids and further understanding of this might eventually lead to better disease indicators or the development of therapeutic potentials that might eventually be used in the management of uterine fibroids.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2019-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.12659/MSMBR.914019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37031450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Rosmarinic Acid Analogue-11 Induces Apoptosis of Human Gastric Cancer SGC-7901 Cells via the Epidermal Growth Factor Receptor (EGFR)/Akt/Nuclear Factor kappa B (NF-κB) Pathway. 迷迭香酸类似物-11 通过表皮生长因子受体 (EGFR)/Akt/Nuclear Factor kappa B (NF-κB) 通路诱导人胃癌 SGC-7901 细胞凋亡

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2019-02-25 DOI: 10.12659/MSMBR.913331

Wanting Li, Qing Li, Liqun Wei, Xiaohang Pan, Daohang Huang, Jialiang Gan, Shuangyi Tang

{"title":"Rosmarinic Acid Analogue-11 Induces Apoptosis of Human Gastric Cancer SGC-7901 Cells via the Epidermal Growth Factor Receptor (EGFR)/Akt/Nuclear Factor kappa B (NF-κB) Pathway.","authors":"Wanting Li, Qing Li, Liqun Wei, Xiaohang Pan, Daohang Huang, Jialiang Gan, Shuangyi Tang","doi":"10.12659/MSMBR.913331","DOIUrl":"10.12659/MSMBR.913331","url":null,"abstract":"BACKGROUND According to the latest statistics from the American Cancer Society, there will be 1.73 million cancer cases and more than 600 000 cancer deaths in the United States in 2018, among which there will be 26 240 new cases of gastric cancer and around 10 800 deaths arising from gastric cancer. The objective of this study was to use RAA-11 to intervene in SGC-7901 cells to understand its effects on cell proliferation and apoptosis, and to explore the apoptosis mechanism. MATERIAL AND METHODS MTT assay was used to detect the survival of human gastric mucosal epithelial GES-1 cells and human gastric cancer SGC-7901 cells. Colony formation assay was used to observe the colony forming ability in SGC-7901 cells. The apoptotic rate of SGC-7901 cells was evaluated by Hoechst33258 staining and flow cytometry. qRT-PCR was used to analyze the epidermal growth factor receptor (EGFR) mRNA expression level in SGC-7901 cells. Western blot was used to examine the expression levels of caspase-3, Bcl-2, BAX, EGFR, Akt, p-Akt, and NF-κB in SGC-7901 cells. RESULTS RAA-11 is capable of inhibiting the proliferation and inducing the apoptosis of SGC-7901 cells in a time- and dose-dependent manner. Western blot showed that the expression levels of caspase-3 and BAX were upregulated, while the expression levels of Bcl-2, EGFR, Akt, p-Akt, and NF-κB in the SGC-7901 cells were downregulated. CONCLUSIONS Apoptosis can be induced in SGC-7901 cells by RAA-11, potentially via the EGFR/Akt/NF-κB pathway, indicating that RAA-11 might be a potent agent for cancer treatment.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2019-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.12659/MSMBR.913331","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37155021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

miR-193a-3p Promotes Radio-Resistance of Nasopharyngeal Cancer Cells by Targeting SRSF2 Gene and Hypoxia Signaling Pathway. miR-193a-3p通过靶向SRSF2基因和缺氧信号通路促进鼻咽癌细胞的放射耐药

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2019-02-18 DOI: 10.12659/MSMBR.914572

Lingsuo Kong, Qing Wei, Xianwen Hu, Lanren Chen, Juan Li

{"title":"miR-193a-3p Promotes Radio-Resistance of Nasopharyngeal Cancer Cells by Targeting SRSF2 Gene and Hypoxia Signaling Pathway.","authors":"Lingsuo Kong, Qing Wei, Xianwen Hu, Lanren Chen, Juan Li","doi":"10.12659/MSMBR.914572","DOIUrl":"https://doi.org/10.12659/MSMBR.914572","url":null,"abstract":"BACKGROUND Radio-resistance is an important barrier in nasopharyngeal carcinoma treatment. MicroRNAs are gene expression core regulators in various biological procedures containing cancer radio-resistance. Nevertheless, the clinical association between nasopharyngeal carcinoma and miR-193a-3p/SRSF2 remains unclear. MATERIAL AND METHODS We examined the miR-193a-3p level in radio-sensitive CNE-2 and radio-resistant CNE-1 NPC cell lines, and, based on a literature review, predicted SRSF2 to be the target gene of miR-193a-3p. We explored the expression of SRSF2 at protein and mRNA levels by transfecting either miR-193a-3p-mimic or antagomiR. Finally, we performed signaling pathway analysis to assess the possible role of miR-193a-3p/SRSF2 in signaling pathways. RESULTS miR-193a-3p promotes NPC radio-resistance, and the SRSF2 gene is the direct target for miR-193a-3p in NPC, and thus is negatively correlated with NPC radio-resistance. The hypoxia signaling pathway activity is strongly affected, and it is possible to use the downstream activity of the SRSF2 gene to show the effect of miR-193a-3p on radio-resistance in NPC cells. CONCLUSIONS miR-193a-3p mediates promotion of NPC radio-resistance.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2019-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3e/08/medscimonitbasicres-25-53.PMC6394146.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36963835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Escherichia Coli Outer Membrane Vesicles Induced DNA Double-Strand Breaks in Intestinal Epithelial Caco-2 Cells. 大肠杆菌外膜囊泡诱导肠上皮Caco-2细胞DNA双链断裂。

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2019-02-11 DOI: 10.12659/MSMBR.913756

Zhou Ling, Chen Dayong, Yu Denggao, Wang Yiting, Fang Liaoqiong, Wang Zhibiao

{"title":"Escherichia Coli Outer Membrane Vesicles Induced DNA Double-Strand Breaks in Intestinal Epithelial Caco-2 Cells.","authors":"Zhou Ling, Chen Dayong, Yu Denggao, Wang Yiting, Fang Liaoqiong, Wang Zhibiao","doi":"10.12659/MSMBR.913756","DOIUrl":"https://doi.org/10.12659/MSMBR.913756","url":null,"abstract":"BACKGROUND Recent studies have shown that Escherichia coli induced digestive tract diseases may be related to outer membrane vesicles (OMVs) induced intestinal double-strand breaks (DSBs) in intestinal epithelial cells. This study aimed to compare the impact of OMVs forces on DSBs in intestinal epithelial Caco-2 cells, and provide a new treatment for digestive diseases caused by E. coli. MATERIAL AND METHODS E.coli OMVs were prepared and co-cultured with Caco-2 cells. The uptake of OMVs by Caco-2 cells was observed by confocal microscopy. The γ-H2AX protein was detected by western-blots. The DSBs caused by OMVs was detected by single cell gel electrophoresis. RESULTS The particle size analyzer showed that the average diameters of OMVs centrifuged at 20 000×g and 50 000×g were 217.5±7.29 nm and 186.3±6.59 nm (P<0.05), respectively. Transmission electron microscopy of the OMVs revealed a lipid bilayer structure with a variety of different sizes. Confocal fluorescence microscopy revealed that OMVs almost completely entered Caco-2 cells after 24 hours. The ratio of γ-H2AX protein band gray value normalized data in the OMVs centrifuged at 20 000×g and 50 000×g, and the control group (without OMVs) were 2.23±0.18, 1.58±0.20, 1±0.30 (P<0.05), respectively, while DNA levels of the comet tail (TailDNA%, TDNA%) were 72.21±14.61%, 23.11±4.98%, and 1.02±1.41% (P<0.05), respectively. The corresponding DNA damage was categorized as high (grade 3), moderate (grade 2), and no damage (grade 0). CONCLUSIONS Different sizes of OMVs induced different degrees of DNA damage in intestinal epithelial Caco-2 cells.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2019-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c4/76/medscimonitbasicres-25-45.PMC6381811.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36948424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6