Methods in molecular medicine最新文献_第2页

Studies of enzymes that cause resistance to aminoglycosides antibiotics. 对氨基糖苷类抗生素产生耐药性的酶的研究。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_20

Engin H Serpersu, Can Ozen, Edward Wright

引用次数: 16

eConsulting. eConsulting.

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-60327-148-6_19

Siaw-Teng Liaw, Peter Schattner

引用次数: 0

Evaluating DNA sequence variants of unknown biological significance. 评估未知生物学意义的DNA序列变异。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-60327-148-6_11

Scott A Grist, Andrew Dubowsky, Graeme Suthers

{"title":"Evaluating DNA sequence variants of unknown biological significance.","authors":"Scott A Grist, Andrew Dubowsky, Graeme Suthers","doi":"10.1007/978-1-60327-148-6_11","DOIUrl":"https://doi.org/10.1007/978-1-60327-148-6_11","url":null,"abstract":"Increasingly, the molecular genetics laboratory has to assess the biological significance of changes (variants) in a DNA sequence. Using the large genes BRCA1 and BRCA2 as examples, some approaches used to determine the biological significance of DNA variants are described. These include the characterization of the variant through a review of the literature and the various databases to assess if it has previously been described. Potential difficulties with the various databases that are available are described. Other considerations include the co-inheritance of the variant with other DNA changes, and its evolutionary conservation. Determining the possible effect of the variant on protein function is described in terms of the Grantham assessment as well as identifying functional domains. Studies looking at the distribution of the variant in both the population and the family can also help in assessing its significance. Loss of the variant in a tumor sample would imply that it is not deleterious. Ultimately, it is not any single parameter that helps determine the DNA variants biological significance. Usually this requires multiple lines of evidence.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-60327-148-6_11","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27418341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Developing a DNA variant database. 开发DNA变异数据库。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-60327-148-6_12

David C Y Fung

{"title":"Developing a DNA variant database.","authors":"David C Y Fung","doi":"10.1007/978-1-60327-148-6_12","DOIUrl":"https://doi.org/10.1007/978-1-60327-148-6_12","url":null,"abstract":"Disease- and locus-specific variant databases have been a valuable resource to clinical and research geneticists. With the recent rapid developments in technologies, the number of DNA variants detected in a typical molecular genetics laboratory easily exceeds 1,000. To keep track of the growing inventory of DNA variants, many laboratories employ information technology to store the data as well as distributing the data and its associated information to clinicians and researchers via the Web. While it is a valuable resource, the hosting of a web-accessible database requires collaboration between bioinformaticians and biologists and careful planning to ensure its usability and availability. In this chapter, a series of tutorials on building a local DNA variant database out of a sample dataset will be provided. However, this tutorial will not include programming details on building a web interface and on constructing the web application necessary for web hosting. Instead, an introduction to the two commonly used methods for hosting web-accessible variant databases will be described. Apart from the tutorials, this chapter will also consider the resources and planning required for making a variant database project successful.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-60327-148-6_12","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27418342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Collection of air samples to quantify exposure to airborne allergens. 收集空气样本以量化空气中过敏原的暴露。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_17

Susan Gordon

引用次数: 3

Determining MHC restriction of T-cell responses. 测定MHC对t细胞反应的限制。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_6

Mark Larché

{"title":"Determining MHC restriction of T-cell responses.","authors":"Mark Larché","doi":"10.1007/978-1-59745-366-0_6","DOIUrl":"https://doi.org/10.1007/978-1-59745-366-0_6","url":null,"abstract":"T-cell receptors (TcR) recognize short linear peptides (9-15 amino acid long), which have been processed by an 'antigen-presenting cell' and complexed to products of the major histocompatibility complex (MHC). Peptides of the appropriate shape and charge are able to bind within the groove of the MHC molecule and it is this complex which is recognized by the TcR. The MHC molecules are highly polymorphic, but each individual will only express one maternal and one paternal allele of HLA Class 1 molecule A, B, and C and HLA Class II DR, DP, and DQ. As a result of TcR specificity and MHC restriction, a clone of T cells will bear a specific receptor which has a very limited repertoire of targets (peptide-MHC complexes). When sufficient numbers of TcR are engaged on a T-cell surface, a cascade of signal transduction events is initiated which results in cellular activation. When investigating the immune response, it may be advantageous to be able to identify which MHC molecules bind a particular peptide well and give rise to a vigorous T-cell response. Such information may be useful in generating effective vaccines. In this chapter, we provide examples of Epstein-Barr virus-transformed lymphoid cell lines and fibroblast cell lines to determine MHC restriction elements.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-59745-366-0_6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27524492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Microarrays--planning your experiment. 微阵列——规划你的实验。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-60327-148-6_5

J. Yang

引用次数: 1

High-throughput screening of peptide deformylase inhibitors. 肽去甲酰基酶抑制剂的高通量筛选。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_10

Kiet T Nguyen, Dehua Pei

引用次数: 4

Methods for assessing the structure and function of cationic antimicrobial peptides. 评价阳离子抗菌肽结构和功能的方法。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_13

Michelle Pate, Jack Blazyk

{"title":"Methods for assessing the structure and function of cationic antimicrobial peptides.","authors":"Michelle Pate, Jack Blazyk","doi":"10.1007/978-1-59745-246-5_13","DOIUrl":"https://doi.org/10.1007/978-1-59745-246-5_13","url":null,"abstract":"Widespread resistance to antibiotics in current clinical use is increasing at an alarming rate. Novel approaches in antimicrobial therapy will be required in the near future to maintain control of infectious diseases. An enormous array of small cationic peptides exists in nature as part of the innate defense systems of organisms ranging from bacteria to humans. For most naturally occurring linear peptides, such as magainins and cecropins, a common feature is their capacity to form an amphipathic alpha-helix (with polar and nonpolar groups on opposite faces of the helix), a structural feature believed to be important in their antimicrobial function as membrane-lytic agents. A massive effort over the past two decades has resulted in a better understanding of the molecular mechanism of antimicrobial peptides and the production of more potent analogues. To date, however, few of these peptides have been shown to have clinical efficacy, especially for systemic use, in large part due to insufficient selectivity between target and host cells. Recently, we developed a new strategy in the design of antimicrobial peptides. These linear cationic peptides, which form amphipathic beta-sheets rather than alpha-helices, demonstrated superior selectivity in binding to the lipids contained in bacterial vs. mammalian plasma membranes. Here we describe methods to evaluate the structure and function of cationic antimicrobial peptides.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-59745-246-5_13","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27404786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Screening for compounds that affect the interaction between bacterial two-component signal transduction response regulator protein and cognate promoter DNA. 影响细菌双组分信号转导反应调节蛋白与同源启动子DNA相互作用的化合物筛选。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_17

Matthew G Erickson, Andrew T Ulijasz, Bernard Weisblum

引用次数: 1