Microbiology Research Journal International最新文献

{"title":"A Rapid Method to Screen for Multi Drug Resistance (MDR) Trait in Clinical Isolates of Acinetobacter baumannii-Acyl Homoserine Lactone (AHL) Screening in CRAB and CSAB Isolates","authors":"Saipriya Kamaraju, V. Sritharan","doi":"10.9734/mrji/2023/v33i31370","DOIUrl":"https://doi.org/10.9734/mrji/2023/v33i31370","url":null,"abstract":"Aims: Our proposal aimed to evaluate Acyl Homoserine Lactones (AHL) as a functional marker for Multi drug resistant (MDR) potential in clinical isolates of Acinetobacter baumannii. We investigated the AHL production potential of clinical isolates using a biosensor assay directly on a commonly used agar media. \u0000Place and Duration of Study: Department of Molecular Diagnostics and Biomarkers, Gleneagles Global Hospitals, Lakdikapul, Hyderabad-500004. \u0000Methodology: Antimicrobial drug sensitivity testing (AST) was performed on 72 clinical isolates of A. baumannii against two front-line antibiotics, Imipenem (10µg) and Meropenem (10µg), by Kirby-Bauer disk diffusion method. Production of long chain Acyl Homoserine lactone (AHLs) in the clinical isolates of A. baumannii was tested by cross streaking with the biosensor Chromobacterium violaceum mutant strain CV026 and Agrobacterium tumefaciens (NTL4pZLR4) by agar plate diffusion assay. Screening and identification of the quorum sensing mediator gene abaI was done by PCR to confirm its presence in all the 72 clinical isolates. \u0000Results: Out of the 72 clinical isolates, 58 were Carbapenem resistant Acinetobacter baumannii (CRAB) and 14 were Carbapenem sensitive Acinetobacter baumannii (CSAB) for AST by agar disc diffusion method. None of our isolates produced short chain AHLs whereas all the isolates could produce varying amounts of long chain AHLs. Genotypic confirmation of AHL gene was obtained by abaI gene PCR. \u0000Conclusion: Carbapenems are the front-line antibiotics used to treat gram negative bacterial infections in emergencies and in the critical care units of hospitals. Clinical isolates A. baumannii has innate resistance to several antibiotics due to various mechanisms, biofilms forming the first line of defense against antibiotics for the bacterium. Our study used AST to carbapenem as the leading marker for MDR, assuming the innate resistance of A. baumannii to other beta lactam antibiotics. Our study brought out certain important observations namely: a) All clinical isolates of A. baumannii produced Quorum Sensing signal molecules, the AHLs b) the clinical isolates of A. baumannii did not produce any short chain AHLs b) All the clinical isolates of A. baumannii produced long chain AHLs c) AHL production is not specific to carbapenem drug resistance because even CSAB isolates produced AHL d) AHL production is inherent to all clinical isolates of A. baumannii and it apparently indicates an underlying biofilm potential and MDR trait in these A. baumannii isolates. e) AHLs could be a universal marker for revealing MDR trait and biofilm potential in clinical microbiology AST profiling protocols. ","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85334856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peste Des Petits Ruminants: An Update","authors":"O. Z. Tenuche, B. O. Emikpe, E. Godwin, S. I. Enem, G. O. Egwu","doi":"10.9734/mrji/2023/v33i31369","DOIUrl":"https://doi.org/10.9734/mrji/2023/v33i31369","url":null,"abstract":"Peste des petits ruminants (PPR) is a notifiable and reportable disease recognised by the Office Internationale Epizooties (OIE) and penned for eradication by 2030. It is an endemic and devastating disease of small ruminants in some countries of Africa, Asia, and the Middle East. The disease militates against small ruminant production and increased flock numbers in the region.  Information and literature on the current review was especially obtained and compiled from CABI abstract data base, PubMed, Scopus, Elibrary USA and other online publications. This review highlights the viral structure, epidemiology, predisposing factors, clinical signs, pathology, immunoprophylaxis, diagnostic techniques and therapeutics of the disease with a view to generating increased scientific research interests on PPR. It is hoped that more robust scientific research breakthroughs could emerge particularly in the area of immunoprophylaxis, rapid penside diagnosis, and alternative therapeutics such as the use of probiotics. This will in no small measure boost small ruminant production, alleviate poverty and increase food security in countries endemic with the disease.","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76197444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological Assessment of Critical Areas in Three Selected Units in Jodhpur Dental College General Hospital, Jodhpur Rajasthan, India","authors":"H. S. Murjanatu, I. Maryam, A. Y. Ahmed, S. Saeed, P. R. Anam, L. D. Rogo","doi":"10.9734/mrji/2023/v33i31368","DOIUrl":"https://doi.org/10.9734/mrji/2023/v33i31368","url":null,"abstract":"The hospital environment plays a crucial role in the chain of infection spread. Thus, there is need to attack the chain of infection at its weakest link as the most effective way in combating and/or preventing nosocomial infections. The study aims to assess the common aerobic pathogenic bacteria in the different departments of Jodhpur National University Dental Hospital and to establish the best disinfectant as well as disinfection procedure. A cross sectional descriptive study was conducted.  Air and surface samples were taken before and after dental procedures from all the three departments within durations of 14 days. Different antiseptics were used so as to compare their effectiveness. Swabs taken from different places were streaked on blood agar plates and incubated at 37oC under aerobic conditions for 24 hours. After incubation, isolates obtained were appropriately identified. After aerobic incubation of the settle plates at 370C for 24hours, the colonies on each plate were counted and recorded as the number of bacteria carrying particles settling over the area of the plate in a given period of time. The level of bacterial contamination of air is usually expressed as the number of bacteria carrying particles per cubic millimeter. A total of 274 surface samples and 97 air samples were collected. Bacteria were isolated in all air samples while only 255 surface samples had growth. The predominant species in all services was Bacillus spp, followed by coagulase negative Staphylococci, Micrococcus luteus, aerobic spore formers and least was Pseudomonas aeruginosa.  The bacteria isolated in the air were similar to those isolated from surfaces. In conclusion, lack of a universal procedure for surveillance of nosocomial infection, presence of pathogenic bacteria, poor hand hygiene and heavy contamination of some important surfaces are the most important problems in our hospitals.","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89560775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiochemistry and Microbiological Quality of Surface Water Body around Port Terminals in Southern Nigeria","authors":"D. Ogbonna, E. Anthony, L. Kpormon","doi":"10.9734/mrji/2023/v33i21367","DOIUrl":"https://doi.org/10.9734/mrji/2023/v33i21367","url":null,"abstract":"Anthropogenic activities around the sea ports are capable of causing changes on the physicochemical and microbiological quality of water bodies along the port terminals. Such activities can cause an ecological imbalance in the water quality /ecosystem resulting in extinction of aquatic resources. The aim of this study therefore was to investigate the physicochemical and microbiological quality of surface water along the busy port terminals. Surface water samples were collected from Onne port terminal using sterile containers. The samples were collected during the wet and dry seasons between January to June 2021. The sterile bottles were filled with surface water samples and transported in an ice packed container to the Department of Microbiology Laboratory of the Rivers State University for analyses using standard analytical methods. Statistical analyses were carried out using ANOVA and All pairs tukey-kramer. Results of the physicochemical parameters showed that temperature, total dissolved solids, total suspended solids, nitrate and heavy metals were significantly higher during the dry season than the wet season at P ≥ 0.05 levels of significance. Seasonal variation with respect to microbial counts shows that Total Heterotrophic Bacteria, Total Heterotrophic Fungi, Total coliforms and Faecal coliforms had  a mean value of 3.9±1.77 x 106; 0.8 ±0.05 x 104 ; 7.4 ±1.3 x 104 and 3.6 ±0.17 x 104 colony forming unit per millilitre  respectively for wet season while the dry season had 1.6±0.77 x 106 , 0.5 ±0.01 x 104 , 4.6 ±0.17 x 104   and 2.7 ±1.03 x 104  cfu/ml respectively. In this study, the predominant bacterial isolates belonged to the genera of Vibrio, Pseudomonas, Klebsiella, Bacillus, Shigella, Staphylococcus, Salmonella, Proteus, Bacillus and Escherichia. coli. The results of physicochemical and microbiological characteristics including the heavy metals, were detected at concentrations on or below detection limits.. It is therefore suggested that relevant environmental regulatory bodies should maintain regular check to ensure that appropriate standards are maintained around seaports due to beehive of activities.","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83308527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo Antisalmonellal and Antioxidant Activity of Hydroethanolic Extract of Bauhinia rufescens Leaves in Wistar Albino Rats Infected with Salmonella Typhi","authors":"J. Djenguemtar, M. Goka, M. Noubom, E. Konack, G. T. Kamsu, J. B. Sokoudjou, H. B. L. Feudjio, N. Kodjio, D. Gatsing","doi":"10.9734/mrji/2023/v33i21366","DOIUrl":"https://doi.org/10.9734/mrji/2023/v33i21366","url":null,"abstract":"Infectious diseases such as typhoid fever lead to the formation of free radicals which can have a detrimental effect on the body. Typhoid fever is caused by poor sanitation, lack of clean water and resistance of germs to antibiotics and reactive oxygen species (ROS). Hence an urgent needs to find alternative treatments with little or no toxicity for the treatment of this disease.\u0000Objective: This work aims to evaluate the in vivo antisalmonellal and antioxidant activity of the 95% hydroethanolic extract of the leaves of Bauhinia rufescens (Fabaceae) in rats experimentally infected with Salmonella Typhi ATCC6539, as an alternative therapy.\u0000Methods: The rats were randomly divided into twelve groups (six per sex) of animals. Thus 3 control groups: (T0) uninfected and untreated; (T-) infected and untreated; (T+) infected and treated with ciprofloxacin (14 mg/kg) and 3 test groups: T1, T2 and T3 infected and treated with different doses of the extract (40, 80 and 117.71 mg/kg respectively). The evolution of the infection and the effectiveness of the treatment were monitored by blood culture, food consumption and weight growth were assessed during the trial; at the end of which the animals were sacrificed and the different parameters were evaluated.\u0000Results: Infected animals treated with different doses of the extract showed zero bacterial loads from the twelfth day post infection in both sexes. Regardless of sex, animals treated with the extract at the dose of 117.71mg/Kg were cured by the seventh day after the start of treatment while those treated with the doses of 40 mg/Kg and 80 mg/kg were cured by the ninth day after the start of treatment. Infection induced a significant (p < 0.05) decrease in food consumption and weight growth, while treatment induced, at all doses, an increase in food consumption and weight growth. Infection also caused a significant (p < 0.05) increase in NO and MDA levels, as well as a significant decrease in catalase and peroxidase activities in animal tissue homogenates. However, treatment resulted in a significant decrease in NO and MDA levels, and a significant (p < 0.05) increase in catalase and peroxidase activities.\u0000Conclusion: These results showed that the 95% hydroethanolic extract of Bauhinia rufescens leaves has mixed antisalmonellal and antioxidant activity in vivo and could be developed for the treatment of typhoid fever.","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81757748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Nickel in Selected Surface Waters of the Bonaberi Industrial Zone, Douala IV Council, Littoral Cameroon","authors":"Nkwenti Peter, Etame Loe Gisele Marie, Ndzobo Ndzana Emmanuel Joel, N. Nga, Bih Larrisse Ntum","doi":"10.9734/mrji/2023/v33i21365","DOIUrl":"https://doi.org/10.9734/mrji/2023/v33i21365","url":null,"abstract":"Aims: To assess the source and level of Nickel in selected surface waters and its impact on the community of the Bonaberi industrial zone. \u0000Study Design: Cross-sectional study design that involved semi-quantitative and qualitative data collection method. \u0000Place and Duration of Study: This study was carried out in the area of Bonaberi industrial zone, Douala IV district between February 2019 to August 2020. \u0000Methodology: 25 Different surface water samples were collected from 5 sampling sites. Water samples were collected in polyethylene bottles previously washed with deionized water and rinsed with sample to be collected from different sites and acidified with 5 ml concentrated nitric acid. The contents of the metal were analyzed using Colorimetric merck microquant procedure. Two way ANOVA test and a P-value (<0.05) was considered significant. \u0000Results: Spring Water had a significant concentration of nickel in the various sites (0.06 mg/L), industrial waste (0.05 mg/L), River (0.04 mg/L), Well (0.03 mg/L), Tap waters (0.02 mg/L). \u0000Conclusion: Nickel is one of the most toxic naturally occurring metal(s) that is very dangerous to environmental sustainability when present in high concentrations. The study justifies the need to ascertain the long-term effects of Nickel contaminant(s) at waste dumping sites. Strengthening waste management systems and water quality monitoring should be implemented in the watersheds to minimize the health effects and deterioration of the aquatic ecosystem. Adequate measures should be taken to educate the community on heavy metals pollution on surface water and their effects to health and environment.","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"125 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89173430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Knowledge Based on Aflatoxin Poisoning among Broiler Farmers in Nairobi City County, Kenya","authors":"Kirinyet Ruth Chepkosgei, Warutere Peterson Njogu, Nguhiu Purity, Ojola Patroba, Kirinyet Joel, Koskei Peter","doi":"10.9734/mrji/2023/v33i21364","DOIUrl":"https://doi.org/10.9734/mrji/2023/v33i21364","url":null,"abstract":"Background: There is a scarcity of information concerning knowledge of aflatoxin contamination of feeds among farmers even in aflatoxin-prone regions in Kenya. Thus, knowledge of aflatoxins in feeds among poultry farmers is of paramount importance in designing plans to minimize risks of aflatoxin exposure. Therefore, this study sought to assess the Determinants of Knowledge on Aflatoxin Among Broiler farmers in Nairobi City County, Kenya. \u0000Methodology: The study utilized an analytical cross-sectional study design. A total of 240 farmers were sampled from a population of 600 farmers within Nairobi City County. A structured questionnaire was administered to farmers within Nairobi City County. SPSS version 26 was used to analyze the data descriptively. Results were presented in tables and figures. Ethical approval was sought from relevant authorities and parties before the commencement of the study. \u0000Results: Results from the study show that the majority of the farmers (58.2%) had knowledge of aflatoxin. There was a significant association (p<0.05) between the socio-demographic characteristics of farmers and knowledge of aflatoxin. \u0000Conclusion: The study concludes that the farmers had adequate knowledge of aflatoxin occurrence in feeds and methods to reduce the contamination. There is a need for continuous sensitization of farmers on aflatoxin, particularly on feed management practices by the Ministry of Agriculture and Ministry of Health Division of Public Health in Kenya.","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89082062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Candida Bloodstream Infection and Antifungal Susceptibility Over Three Years in a Single Center from Medinah, Saudi Arabia","authors":"Z. Alahmadey, Sultan S. Al-ahmadi, Eid D. Aljohani, Nader H. Al-Rashidi","doi":"10.9734/mrji/2023/v33i21363","DOIUrl":"https://doi.org/10.9734/mrji/2023/v33i21363","url":null,"abstract":"In the healthcare setting, Candida bloodstream infections significantly increase morbidity and mortality. There is little proof that invasive infections in Saudi Arabia are brought on by Candida spp. To identify Candida species that cause bloodstream infections and ascertain the clinical outcome and risk factors for mortality in a Saudi Arabian tertiary hospital. This retrospective analysis covered all instances in which patients hospitalized to Ohud hospital, a tertiary care facility in Madinah, Saudi Arabia, between January 2019 and December 2021, had positive blood cultures for Candida. Anaerobic and aerobic Bactec bottles were inoculated with blood samples and then incubated at 35°C for five days. Identification-YST card kits from VITEK II (BioMerieux, France) for yeast and yeast-like organisms. Testing for antifungal susceptibility was done using AST YS07. A total of 78 patients (71% men, 29% women) were found to have candidemia. Candida albicans (51.3%), Candida parapsilosis (16.7%), and Candida tropicalis (16.7%) were the three Candida spp. that were most frequently isolated. Those with Saudi (51%; P = 0.500), leukopenia (40%; P = 0.001), neutrophilia (92%; P = 0.638), and thrombocytopenia (42%; P = 0.374) had a higher incidence of candidemia. Fluconazole sensitivity in non-albicans Candida species was 39.5%. Nonetheless, caspofungin was effective against all species. This study discovered an epidemiological shift toward more non-albicans Candida spp. in Saudi Arabia as well as a changing pattern in the Candida spp. causing bloodstream infections.","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"433 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78129921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enumeration of Salmonella Species Isolated in Ready–to–Eat Fruits Vended in Bukuru Market, Jos South, Plateau State","authors":"M. Ramyil, A. F. Umar, T. Inusa, M. Y. Iliyasu, T. Ogundeko, C. Bello, A. Bassi, E. Agbo","doi":"10.9734/mrji/2023/v33i11362","DOIUrl":"https://doi.org/10.9734/mrji/2023/v33i11362","url":null,"abstract":"Background: Despite control measures to curtailed salmonella fruit contamination over the years, pathogenic disease outbreaks caused by the ingestion of Salmonella contaminated fresh-cut-fruits pose a significant problem to human health by the consumption of fresh and minimally processed fruits. This study aimed to enumerate and determine the prevalence of Salmonella species isolated in ready-to-eat fruits vended in Bukuru Market Jos South, Plateau state. \u0000Methodology: A total of seventy-eight ready-to-eat vended fruit samples were purchased and cultured for the enumeration of bacterial isolates according to National Food Safety Standard for Microbiological Examination. Pulp pH value of each fruit was obtained by immersing litmus paper into the pulp and results recorded. \u0000Results: Of the 78 fruits specimen, 22 (28.2%) were Salmonella positive. The prevalence rates of salmonella isolated were found to be higher (22.7%) in both coconut and avocado followed by watermelon (18.2%) and sweetmelon (13.6%) as compared with other fruits in the study area. Lower rates of 4.5% were found in banana, pawpaw, and dates with a rise in apple with 9.1% respectively. \u0000Conclusion: The study showed a high p<0.05 (7.811) prevalence of pathogenic Salmonella species isolated in ready-to-eat fruits in the study area revealing that the spread of salmonella is not independent of fruits thereby suggestive of contamination made available by fruit vendors in this part of the world. Thus, epidemiological traceability and significant measures must be taken to check the safety of these vended products before consumption.","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90646798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal Variation of Mycorrhizal Association (Root Colonization and Spore Density) in Selected Industrial Sites in Kota District of Rajasthan, India","authors":"S. Rajpurohit, P. Jaiswal","doi":"10.9734/mrji/2023/v33i11361","DOIUrl":"https://doi.org/10.9734/mrji/2023/v33i11361","url":null,"abstract":"This paper aims to assess the impact of seasonal variation on mycorrhizal association in selected industrial wastelands in the Kota district of Rajasthan. In the study mycorrhizal association was quantified in terms of percentage root colonization and spore density in three different seasons.  The study was conducted during 2019-2021 and the data was collected for 3 seasons; summer (March-June), Rainy (July–October), and winter (November-February) to study the response to variable climatic factors to the root colonization and spore density of mycorrhiza. To determine the significance of variations in AMF spore density and percentage root colonization during different seasons, one-way ANOVA was performed. Results showed that in all the sites maximum mean spore density was observed in the summer (March-June) season but in the case of percentage root colonization, the value was found maximum in the humid season (July-October) in the control site whereas, in the three experimental sites, the maximum root colonization was observed in summer season (march-June).  Hence, it may be concluded that hot climatic condition is favourable for mycorrhizal spore formation and root colonization was also favoured by hot climate.","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"142 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77786654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}