Mediators of Inflammation最新文献

{"title":"Strain-Dependent Differences in Inflammatory/Immune Activity in Cutaneous Wound Tissue Repair in Rats: The Significance of Body Mass/Proneness to Obesity.","authors":"Jelena B Kulas, Aleksandra D Popov Aleksandrov, Dusanka D Popovic, Anastasija Lj Malesevic, Maja M Cakic Milosevic, Milena V Kataranovski, Ivana I Mirkov, Dina M Tucovic","doi":"10.1155/mi/5525557","DOIUrl":"10.1155/mi/5525557","url":null,"abstract":"<p><p>Inflammatory/immune cells and mediators are substantial for wound healing because they orchestrate biological activities in this complex process. Among factors that affect wound healing, obesity, and metabolic diseases are among the most significant, particularly because of a relationship between obesity and a prediabetic state with immune reactivity. Using Dark Agouti (DA) and Albino Oxford (AO) rats, which differ in immune responses as well as in proneness to obesity, we examined the impact of these intrinsic factors on cutaneous wound healing. Dynamics of the process were monitored at days 3, 5, and 7 post-wounding parallel in both rat strains by analysis of selected basic aspects of the wound repair process (cytokine and growth factor responses) in granulation tissue. Strain-related differences in the extent of reduction of the wound area were shown, which coincided with differential proinflammatory and immune-regulatory cytokines, as well as growth factors response in these rats. Some of these differences seem related to their dissimilarities in the proneness to obesity. Results in this study extended so far known differences in inflammatory/immune responses to a variety of stimuli between AO and DA rats and showed, for the first time, immune-based differences in wound healing between rats that differ in body mass (BM) and obesity proneness (under ad libitum feeding conditions with normal rodent chow).</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2025 ","pages":"5525557"},"PeriodicalIF":4.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterogeneity of Neutrophils and Immunological Function in Neonatal Sepsis: Analysis of Molecular Subtypes Based on Hypoxia-Glycolysis-Lactylation.","authors":"Huabin Wang, Ru Yang, Nan Chen, Xiang Li","doi":"10.1155/mi/5790261","DOIUrl":"10.1155/mi/5790261","url":null,"abstract":"<p><p><b>Objective:</b> Hypoxia-glycolysis-lactylation (HGL) may play a crucial role in neonatal sepsis (NS). This study aims to identify HGL marker genes in NS and explore immune microenvironment among NS subtypes. <b>Materials and Methods:</b> The gene expression dataset GSE69686, comprising 64 NS cases and 85 controls, was selected for analysis. Based on the screened HGL-related marker genes, diagnostic prediction models were constructed using nine machine learning algorithms, and molecular subtypes of NS were identified through consensus clustering. Subsequently, the heterogeneity of biological functions and immune cell infiltration among the different subtypes was analyzed. Finally, the marker genes and lactylation were validated using the GSE25504 dataset, clinical samples, and mouse neutrophil, respectively. <b>Results:</b> MERTK, HK3, PGK1, and STAT3 were identified and validated as marker genes, and the diagnostic prediction model for NS constructed using the support vector machine (SVM) algorithm exhibited optimal predictive performance. Based on gene expression patterns, two distinct NS subtypes were identified. Functional enrichment analysis highlighted significant immune-related pathways, while immune infiltration analysis revealed differences in neutrophil proportions between the subtypes. Furthermore, the expression levels of marker genes were positively correlated with neutrophil infiltration. Importantly, the experimental validation results were consistent with the findings from the bioinformatics analysis. <b>Conclusion:</b> This study identified the distinct NS subtypes and their associated marker genes. These findings will contribute to elucidating the disease's heterogeneity and establishing appropriate personalized therapeutic approaches.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2025 ","pages":"5790261"},"PeriodicalIF":4.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Salivary and Serum Inflammatory Mediator Profiles in Patients With Rheumatoid Arthritis and Periodontitis.","authors":"Carina Fei, Kaja Eriksson, Guozhong Fei, Luis Fernando Delgado Zambrano, Shigufta Syed, Caroline Lindström, Martina Ericson, Mehrad Mohammadi, Georgios Tsilingaridis, Amel Guenifi, Rikard Holmdahl, Leif Jansson, Tülay Yucel-Lindberg","doi":"10.1155/mi/7739833","DOIUrl":"10.1155/mi/7739833","url":null,"abstract":"<p><p><b>Background:</b> Periodontitis (PD) and rheumatoid arthritis (RA) are chronic inflammatory conditions, characterized by dysregulated immune response and excessive production of inflammatory mediators. The oral disease PD is triggered by periodontal pathogens, leading to the destruction of tissues surrounding the teeth, whereas RA is a systemic autoimmune disease primarily affecting the joints. The objective of this study was to investigate the prevalence of PD and map the profile of salivary and serum inflammatory mediators of patients with RA, with respect to periodontal severity (PD stage II and PD stage III/IV). <b>Methods:</b> For this cross-sectional cohort study, 62 patients diagnosed with RA were recruited. All participants underwent a full-mouth dental examination. Levels of various inflammatory mediators, including tumor necrosis factor (TNF) superfamily proteins, interferon (IFN) family proteins, regulatory T cell (Treg) cytokines, and matrix metalloproteinases were determined in saliva and serum samples from each participant using a human inflammation multiplex immunoassay panel. <b>Results:</b> In the current RA cohort, all participants were diagnosed with PD, of which 35.5% were classified as PD stage II and 64.5% as PD stages III/IV. Inflammatory mediator levels were significantly higher in both saliva and serum samples from patients with RA and PD stages III/IV, compared to those with RA and stage II within the same cohort. These included higher serum levels of sCD30, IL-10, IL-19, osteopontin and elevated salivary levels of BAFF/TNFSF13B and IFN-<i>α</i>2. Additionally, APRIL/TNFSF13 levels were increased in both saliva and serum. <b>Conclusions:</b> Among the studied patients with RA, the majority exhibited severe PD (stage III/IV), underscoring the importance of periodontal prophylaxis and treatment for this group of patients. Higher levels of inflammatory mediators were observed in both saliva and serum in those with PD stages III/IV, suggesting a potential link between the severity of PD and systemic inflammation in RA. Further research is needed to explore the clinical implications of these findings.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2025 ","pages":"7739833"},"PeriodicalIF":4.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Histone Deacetylase Activator ITSA-1 Improves the Prognosis of Cardiac Arrest Rats by Alleviating Systemic Inflammatory Responses Following Cardiopulmonary Resuscitation.","authors":"Chenyu Zhang, Hongyan Wei, Qiang Zhang, Haohong Zhan, Yuanzheng Lu, Yujie Li, Bo Li, Wen Huang, Feng Nian, Rong Liu, Chunlin Hu, Jie Chen","doi":"10.1155/mi/8156593","DOIUrl":"10.1155/mi/8156593","url":null,"abstract":"<p><p><b>Objective:</b> To investigate whether the histone deacetylase (HDAC) activator ITSA-1 can ameliorate systemic inflammation after cardiac arrest (CA), thereby enhancing cardiac function and neurological outcomes in rats. <b>Materials and Methods:</b> Sixty-nine healthy adult male Wistar rats were subjected to 12 min of CA induced by Vecuronium bromide. The rats were randomly assigned to five groups: normal control, sham operation, control, suberoylanilide hydroxamic acid (SAHA), and ITSA-1. The study evaluated the effects of ITSA-1 on cardiac function, survival, and neurological functions, including the neurological deficit score (NDS) at 24-, 48-, and 72-h post-return of spontaneous circulation (ROSC) and Morris water maze performance at 72 h. Additionally, levels of TNF-<i>α</i>, IL-1<i>β</i>, glial fibrillary acidic protein (GFAP), S100<i>β</i> in plasma, and TNF-<i>α</i>, IL-1<i>β</i> in the hippocampus were measured 4 h post-ROSC. Western blot analysis was used to assess HDACs, nuclear factor kappa B (NF-<i>κ</i>B), p-NF-<i>κ</i>B, caspase-3, cleaved caspase-3, Bcl-2, and Bax protein expressions. <b>Results:</b> ITSA-1 reduced basic life support (BLS) duration and adrenaline dosage during cardiopulmonary resuscitation (CPR) and improved cardiac and neural functions, enhancing survival compared to the control and SAHA groups. ITSA-1 decreased serum levels of IL-1<i>β</i>, TNF-<i>α</i>, GFAP, S100<i>β</i>, and hippocampal TNF-<i>α</i>, IL-1<i>β</i>, promoting neuronal survival in the CA1 region. It also inhibited glial cell activation and reduced histone acetylation, blocking the NF-<i>κ</i>B pathway and neuronal apoptosis. <b>Conclusion:</b> ITSA-1 enhances the recovery and survival of post-ROSC rats by diminishing histone acetylation and mitigating systemic inflammation. This effect is possibly due to the inhibition of glial cell activation, increased neuronal survival in the brain, and improved cardiac output (CO) and ejection fraction (EF).</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2025 ","pages":"8156593"},"PeriodicalIF":4.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil-to-Lymphocyte Ratio as a Potential Predictive Marker for Epileptic Seizures: Unveiling the \"V\"-Shaped Link.","authors":"Xianmei Wang, Yang Zhang, Yi Ye, Long Wang, Yi Xu, Siying Ren, Likun Wang, Guofeng Wu","doi":"10.1155/mi/2247724","DOIUrl":"10.1155/mi/2247724","url":null,"abstract":"<p><p><b>Objective:</b> The neutrophil-to-lymphocyte ratio (NLR) is an important marker of inflammation. An increased NLR has been detected in the blood of patients with epilepsy. However, the correlation between varying NLRs and epileptic seizures (ESs) is still unclear. <b>Methods:</b> A retrospective analysis was conducted, and patients were divided into two groups based on whether they had ES upon admission. Comprehensive data were collected, including routine blood tests, demographic information, and medical histories. The NLR was calculated by dividing the percentage of neutrophils by the percentage of lymphocytes. <b>Results:</b> In total, 414 patients were included (284 males, 151 females; aged 18-90 years), with 271 in the experimental group and 143 in the control group. No significant differences in the NLR were found between the groups (<i>p</i>=0.164). Nonetheless, when NLRs were categorized, a \"V\"-shaped link with ES was observed. An NLR of 2-3 correlated with the lowest seizure incidence. Higher NLRs were associated with increased neutrophil and decreased lymphocyte counts. Patients with an NLR < 2 had the lowest infection rates and the highest cerebrovascular disease exposure, whereas those with an NLR > 3 had the highest infection rates and were the oldest. <b>Conclusions:</b> NLR modifications can serve as potential predictive markers for ES. However, the relationship between the NLR and ES is not linear. The factors contributing to these variations are multiple and complex. An NLR of 2-3 may represent an equilibrium point. An elevated NLR indicates pronounced inflammatory responses, while a low NLR can have more complex causes.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2025 ","pages":"2247724"},"PeriodicalIF":4.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Chronobiological and Neuroprotective Mechanisms of Resveratrol in Improving Sleep.","authors":"Wenwen Zhu, Ailin Gong, Bin Zhang, Hanxing Cheng, Lishan Huang, Xiao Wu, Dechou Zhang, Wenbin Dai, Sen Li, Houping Xu","doi":"10.1155/mi/4954030","DOIUrl":"10.1155/mi/4954030","url":null,"abstract":"<p><p>According to statistics, more than one-third of the global population currently experiences sleep problems, and about 10% of adults have been diagnosed with insomnia, a proportion that is increasing annually. Most currently used insomnia medications are not specifically developed but are discovered by chance, often resulting in unavoidable side effects like addiction. Thus, there is an urgent need to find safer and more effective therapeutic options. Resveratrol, a natural polyphenolic compound, shows significant potential in improving insomnia. Research shows that its effects may be achieved through multiple biological processes, including antiapoptosis, antioxidant activity, anti-inflammation, circadian rhythm regulation, modulation of neurotransmitters (gamma-aminobutyric acid (GABA), DA, 5-HT, cortisol), and increased levels of neurotrophic factor BDNF. Additionally, resveratrol's treatment of insomnia is closely linked to the SIRT1, AMPK, NF-<i>κ</i>B, mTOR, PI3K/Akt, and MAPK pathways. This review summarizes the mechanisms of resveratrol in treating insomnia to provide researchers with a deeper understanding of its action, which can aid in the development of novel targeted drugs and offer innovative ideas and methods for clinical insomnia treatment.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2025 ","pages":"4954030"},"PeriodicalIF":4.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Arginine-Loaded Red Blood Cell Nanovesicles Targeting Obese Asthma.","authors":"Quoc Quang Luu, Taejune Kim, Thi Bich Tra Cao, Injung Choi, Seung Yun Yang, Beum-Soo An, Dae Youn Hwang, Youngwoo Choi, Hae-Sim Park","doi":"10.1155/mi/8248722","DOIUrl":"10.1155/mi/8248722","url":null,"abstract":"<p><p><b>Purpose:</b> The role of the gut microbiomes has been emphasized in the pathogenesis of obese asthma (OA). However, the molecular mechanism of airway dysfunction underlying OA has not yet been fully elucidated. The effects of microbiomes on arginine metabolism in relation to lung functions and a novel method for delivering arginine to lung tissue based on arginine-loaded red blood cell (RBC)-derived nanovesicles (NVs) (NV^Arg) will be investigated. <b>Materials and Methods:</b> Inflammatory status, amino acid profiles, and microbial diversity were evaluated in 20 adult patients with OA compared to 30 adult patients with non-OA (NOA) and 10 healthy control (HC) groups. Changes in gut or lung microbial composition that altered arginine metabolism in relation to airway inflammation were investigated in an OA mouse model in vivo. Additionally, this study evaluated the delivery of arginine to lung tissue utilizing NV^Arg in vivo and in vitro. <b>Results:</b> Significantly increased <i>Bacteroides</i> abundance but decreased serum arginine concentration with lower forced exhaled volume at 1 s (FEV₁) (%) was noted in the OA group compared to the NOA and HC groups. In mouse experiments, when OA mice were given living bacteria from normal control (NC) mice, lung arginine concentration and airway resistance were restored. However, the administration of arginine or its metabolite (citrulline) did not increase the arginine levels in the lung tissues. We therefore created NV^Arg, which successfully delivered arginine into the cytoplasm of the airway epithelial cell line <i>in vitro</i>. Oral administration of NV^Arg for OA mice significantly induced the AMP-activated protein kinase (AMPK) and endothelial nitric oxide synthase (eNOS) pathways in airway epithelial cells, which reduced airway resistance and inflammation. <b>Conclusion:</b> These findings suggest that microbiomes contribute to airway dysfunction by regulating arginine metabolism, whereas NV^Arg treatment may be a potential option for managing OA.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2025 ","pages":"8248722"},"PeriodicalIF":4.4,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Association Study Between Cytokines and the Risk for Cerebral Palsy.","authors":"Baotian Wang, Fan Wang, Li Yang, Junhong Jiang, Jiulai Tang, De Wu","doi":"10.1155/mi/3742331","DOIUrl":"10.1155/mi/3742331","url":null,"abstract":"<p><p><b>Background:</b> Cerebral palsy (CP) is a debilitating condition characterized by abnormal movement or posture beginning early in development. Recent evidence has shown that immunological abnormalities are associated with an increased risk of CP. However, there are no valuable biomarkers for CP diagnosis. <b>Methods:</b> In this case-control study, we recruited 108 children with CP and 52 healthy children as controls. The white blood cell (WBC) counts and the levels of inflammatory markers (interleukin-1<i>β</i> (IL-1<i>β</i>), sIL-2R, interleukin-6 (IL-6), IL-8, IL-10, and tumor necrosis factor-<i>α</i> (TNF-<i>α</i>)), neuron-specific enolase (NSE), immunoglobulin E (IgE), and C3/C4 in the blood were measured and the results were statistically analyzed. Subgroup analyzes based on age, complications, and clinical subtypes were also carried out. <b>Results:</b> Compared with the controls, CP patients had elevated levels of NSE, sIL-2R, and TNF-<i>α</i>. There were no differences in WBC count, IL-1<i>β</i>, IL-6, IL-8, IL-10, IgE, C3, or C4. Subgroup analysis revealed significant differences in the personal-social developmental quotient (DQ) among the different CP subtypes. We found that TNF-<i>α</i>, sIL-2R, gross motor DQ, and adaptive DQ were greater in children with CP without epilepsy (EP) than in those with EP. Correlation analysis revealed positive correlations between TNF-<i>α</i> and sIL-2R, gross motor DQ, fine motor DQ, adaptive DQ, and personal-social DQ; moreover, sIL-2R was positively correlated with TNF-<i>α</i>, gross motor DQ, adaptive DQ, personal-social DQ, and eosinophil (EO) count and negatively correlated with age. NSE and TNF-<i>α</i> were associated with a 1.64-fold and 1.66-fold increased risk of CP, respectively. The peripheral blood NSE and TNF-<i>α</i> levels exhibited good diagnostic value for CP. Moreover, receiver operating characteristic (ROC) curve analysis revealed a significant increase in the area under the curve (AUC) when these indicators were combined. <b>Conclusions:</b> This study revealed significant associations between NSE and TNF-<i>α</i> and CP risk, suggesting that NSE and TNF-<i>α</i> might be useful blood biomarkers for identifying patients at high risk of CP.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2025 ","pages":"3742331"},"PeriodicalIF":4.4,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11930382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Faecalibacterium prausnitzii</i> Colonization Attenuates Gut Inflammation and Epithelial Damage in a DSS-Induced Colitis Mice Model.","authors":"Meng-Chuan Liu, Yu-An Shu, Yu-Chin Wang, Hsiu-Ying Tseng, Meng-Jia Li, Yu-Ting Yu, Hsiu-Chi Cheng, Pei-Jane Tsai, Yao-Jong Yang","doi":"10.1155/mi/7280675","DOIUrl":"https://doi.org/10.1155/mi/7280675","url":null,"abstract":"<p><p><b>Background:</b> Reduction of <i>Faecalibacterium prausnitzii</i> abundance is related to inflammatory bowel diseases (IBDs), and supplement of it exists protective effects. <b>Aim:</b> This study aimed to establish a <i>F. prausnitzii</i>-colonized mouse model and investigate that the presence of <i>F. prausnitzii</i> in the gut can ameliorate the severity of dextran sulfate sodium (DSS)-induced colitis. <b>Methods:</b> A <i>F. prausnitzii</i> (ATCC 27768) strain was maintained on the PS-BHI agar plates and manipulated in a strictly anaerobic chamber. A <i>F. prausnitzii</i>-colonized C57BL/6 mice model was tested by a rectal enema with 1 × 10⁹ bacteria/day for 3 days. The 5% DSS was added to drinking water for 3 days to induce colitis and diarrhea in experimental mice. The clinical, cytological, and histological severities were compared between groups. <b>Results:</b> The <i>F. prausnitzii</i>-colonized mice model was successfully established via rectal enema with the property of transfer to offspring. DSS treatment altered gut microbiota and significantly attenuated the abundance of <i>F. prausnitzii</i> in colonized mice. Mice with <i>F. prausnitzii</i> colonization had significantly improved weight loss, anal bleeding, stool consistency, cecum weight, colon length, and serum amyloid A (SAA) level than those without after DSS treatment. Furthermore, the <i>F. prausnitzii</i>-colonized mice significantly reduced the transcription levels of TNF-α, INF-γ and IL-18, and epithelial damage and PMN infiltration in the lamina propria and had better preservation of goblet cells than the control group. <b>Conclusion:</b> We have successfully established a mouse model colonized with <i>F. prausnitzii</i> via rectal enema administration and showed colonization of <i>F. prausnitzii</i> in the gut has a protective effect against DSS-induced colitis.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2025 ","pages":"7280675"},"PeriodicalIF":4.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nomograms Based on Blood-Based Biomarkers for Predicting Prognosis in Locally Advanced Nasopharyngeal Carcinoma Patients.","authors":"Sisi Wang, Yuhua Feng, Jie Ling, Xiayan Zhao, Yanming Hu, Tao Hou, Yangchun Xie","doi":"10.1155/mi/6618728","DOIUrl":"https://doi.org/10.1155/mi/6618728","url":null,"abstract":"<p><p><b>Purpose:</b> This study aimed to investigate the prognostic significance of the platelet-to-platelet distribution width ratio (P/PDW), systemic inflammation response index (SIRI), and systemic immune inflammation index (SII) in patients with locally advanced nasopharyngeal carcinoma (LA-NPC). <b>Methods:</b> A total of 549 LA-NPC patients were included in this retrospective analysis. Clinicopathological characteristics and blood test data were obtained from patient records. Receiver operating characteristic (ROC) curve analysis was employed to determine the optimal cutoff values for P/PDW, SIRI, and SII. The <i>χ</i> ² test was used to compare clinicopathological characteristics. Survival rates were calculated using the Kaplan-Meier method. Prognostic factors were evaluated using univariate and multivariate analyses via Cox regression. Additionally, we developed a nomogram to predict outcomes and assessed its acuracy using the concordance index (C-index) and a calibration curve. <b>Results:</b> The median follow-up time was 47.1 months. Elevated P/PDW levels were associated with advanced N stages and higher risks of disease progression (all <i>p</i>  < 0.05). Patients with high SIRI or SII levels were more likely to have advanced T stages, clinical stages, and to develop metastasis (all <i>p</i>  < 0.05). Univariate analysis revealed that P/PDW, SIRI, SII, and T stage were significantly correlated with both overall survival (OS) and progression-free survival (PFS; all <i>p</i>  < 0.05). Clinical stage was significantly related only to PFS (<i>p</i>=0.009). Multivariate Cox regression analysis identified P/PDW (hazard ratio (HR): 0.544, 95% confidence interval (CI): 0.390-0.759, <i>p</i>  < 0.001; HR: 0.406, 95% CI: 0.268-0.615, <i>p</i>  < 0.001) and T stage (HR: 0.539, 95% CI: 0.378-0.768, <i>p</i>=0.001; HR: 0.545, 95% CI: 0.364-0.815, <i>p</i>=0.003) as independent prognostic factors for both OS and PFS, while SIRI (HR: 0.525, 95% CI: 0.333-0.827, <i>p</i>=0.006) was an independent predictor of OS. Nomogram C-indexes for the nomogram of OS were 0.717 and PFS were 0.711, respectively. Survival predictions and actual survival were consistent according to the calibration curve. <b>Conclusion:</b> Our findings suggest that P/PDW is a convenient and effective marker for predicting outcomes in LA-NPC patients.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2025 ","pages":"6618728"},"PeriodicalIF":4.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}