Meat and Muscle Biology最新文献

{"title":"Transcriptomic analysis for pork color – the ham halo effect in biceps femoris","authors":"D. Nonneman, B. N. Keel, A. Lindholm-Perry, G. Rohrer, T. Wheeler, S. Shackelford, D. King","doi":"10.22175/mmb.13050","DOIUrl":"https://doi.org/10.22175/mmb.13050","url":null,"abstract":"Pork color is a major indicator of product quality that guides consumerpurchasing decisions. Recently, industry has received an increase in consumercomplaints about the lightness and non-uniformity of ham color, primarilylighter color in the periphery termed “ham halo” that is not caused bymanufacturing procedures. This effect is seen in fresh and processed hams andthe outer, lighter muscle is associated with lower myoglobin concentration, pHand type I fibers. The objective of this study was to identify differences ingene expression profiles between light and normal colored portions of biceps femoris muscle from pork hams.RNA-sequencing was performed for paired light and normal colored muscle samplesfrom 10 animals showing the ham halo effect. Over 50 million paired-end reads(2x75bp) per library were obtained. An average of 99.74% of trimmed high-qualityreads were mapped to the Sscrofa 11.1 genome assembly. Differentially expressedgenes (DEGs) were identified using both the DESeq2 and GFOLD software packages.A total of 14,049 genes were expressed in bicepsfemoris; 13,907 were expressed in both light and normal muscle, while 56and 86 genes were only expressed in light and normal muscle, respectively. Analysiswith DESeq2 identified 392 DEGs with 359 genes being more highly expressed innormal colored muscle. A total of 61 DEGs were identified in the DESeq2analysis and also were identified in at least 7 of the 10 individual animalanalyses. All 61 of these DEGs were up-regulated in normal colored muscle. Geneontology (GO) enrichment analysis of DEGs identified the transition betweenfast and slow fibers, and skeletal muscle adaptation and contraction as themost significant biological process terms. The evaluation of gene expression byRNA-Seq identified DEGs between regions of the biceps femoris with the ham halo effect that are associated with thevariation in pork color.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79135570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging understanding of meat flavor for product success","authors":"Rodrigo Tarté","doi":"10.22175/mmb.13045","DOIUrl":"https://doi.org/10.22175/mmb.13045","url":null,"abstract":"In order to get the most benefit out of the use of spices, seasoningsand flavors, an understanding of meat flavor and the factors that affect it isneeded. This article discusses flavor and its sensory perception, the variousfactors that affect it, and how this understanding can be leveraged to achieveformulation objectives and product success.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75518182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cooking surface temperatures, steak thickness, and quality grade effects on volatile aroma compounds","authors":"C. Kerth, M. Berto, R. Miller, J. Savell","doi":"10.22175/mmb.12929","DOIUrl":"https://doi.org/10.22175/mmb.12929","url":null,"abstract":"Beef flavor attributes were evaluated in USDA TopChoice and Select beef top loin steaks cut 1.3 cm (THIN) or 3.8 cm (THICK) andcooked on a commercial flat top grill at 177˚C (LOW) or 232˚C (HIGH) grillsurface temperature. Gas chromatography/mass spectrophotometry, was used toevaluate volatile aroma compounds.  USDASelect steaks had more 2-octene and less trimethyl pyrazine in (P<0.05) THINsteaks than THICK steaks, while Choice was unaffected by steak thickness(P>0.05).  Benzene acetaldehyde washigher and 4-hydroxybenzoic acid was higher in Select LOW grill temperaturescompared to Select HIGH grill temperatures, while 5-methyl-2-furancarboxaldehyde was only present in Choice HIGH grill temperatures (P<0.05).Two acid, three alcohol, one aldehyde, one alkane, and one ketone volatilearoma compounds were higher (P<0.05) for LOW compared to HIGH.  Conversely, five alcohols, two aldehyde, twoalkane, all four furans, six ketones, four pyrazines, along with 1H-indole, twopyrroles, two pyridines, and one benzene aroma compounds were higher (P<0.05)in HIGH compared to LOW.  Additionally,one alcohol, two aldehydes, one ketones, one sulfur-containing, and six othervolatile compounds were lower, while one acid, one alcohol, one aldehyde, twofurans, one ketone, three pyrazine, one sulfur-containing, and one othervolatile compounds were higher in the THIN compared to THICK.  Some aroma compounds like 2-butanone,4-methyl-2-pentanone, 1-ethyl-1H-pyrrole, 1-methyl-1H-pyrrole, and2-methyl-pyridine were only present in THICK cooked HIGH (P<0.05). Steakthickness and grill time are important factors to consider in the developmentof positive Maillard reaction products.@font-face{font-family:\"Cambria Math\";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:swiss;mso-font-pitch:variable;mso-font-signature:-536859905 -1073732485 9 0 511 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:\"\";margin:0in;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:\"Calibri\",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:\"Times New Roman\";mso-bidi-theme-font:minor-bidi;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:\"Calibri\",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:\"Times New Roman\";mso-bidi-theme-font:minor-bidi;}div.WordSection1{page:WordSection1;}@font-face{font-family:\"MS Mincho\";panose-1:2 2 6 9 4 2 5 8 3 4;mso-font-","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79380519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chilling duration on USDA Quality Grade of beef carcasses","authors":"Caitlin Hanes, Kalyn B Coatney, Daniel Rivera, W. Schilling, T. Dinh","doi":"10.22175/mmb.12991","DOIUrl":"https://doi.org/10.22175/mmb.12991","url":null,"abstract":"Two hundred and nine beef carcasses (BW of 361 ± 53 kg) from crossbred, grain-finished cattle were harvested in a commercial abattoir and subjected to a 96-h spray chilling treatment, conducted at 0 to 3°C in a commercial hot box with a wind speed of 3.1 m/s and 153-lux of fluorescent light. At the 24, 48, 72, and 96 h points of the treatment, the carcasses were analyzed for fatty acid composition, marbling score, core temperature (n = 1), pH, shrinkage, color, and aerobic plate count (n = 50). Carcasses reached 3ºC after 12 to 16 h of chilling. There were minimal changes in shrinkage among time point (-0.4 to 1.2%; P ≤ 0.002), pH (5.56 to 5.69; P ≤ 0.001), and aerobic plate count (APC) (0.1 to 0.7 log; P ˂ 0.001). Initial 24-h grading revealed a grade composition of 21.1% Slight (SL, n = 44), 34.0% Small (SM, n = 71), 17.2% Modest (MT, n = 37), 17.7% Moderate (MD, n = 36), and 10.1% Slightly Abundant (SA, n = 21). With marbling score in numeric values between 200 (Practically Devoid00) and 1100 (Abundant00), carcasses that had SM or greater marbling score at 24 h experienced a deduction of 34 to 60 points by the 96th hour of spray chilling (P ≤ 0.042). Comparatively, the marbling scores of the SL carcasses increased from 442 points at 24 h to 469 points at 96 h. Moreover, SL carcasses had a greater percentage of polyunsaturated fats (PUFA) (P < 0.001). Results indicate that spray chilling for 96 h may slightly increase the marbling score of USDA Select, but has minimal impacts on marbling score of greater USDA quality grades.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79156392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of market type and time of purchase on bacterial counts and Salmonella and Listeria prevalence in chicken in Vietnam","authors":"A. Englishbey, Anuraj Theradiyil Sukumaran, P. Vu, Yen Thuc, C. Dahlgren, M. Le, Dzung Nguyen, P. Broadway, L. Guillen, M. Brashears, J. Donaldson, W. Schilling, B. Rude, M. Crenshaw, T. Dinh","doi":"10.22175/mmb.12933","DOIUrl":"https://doi.org/10.22175/mmb.12933","url":null,"abstract":"The objective of the current study was to determine the influence of market type and sampling time on Salmonella and Listeria prevalence and bacterial counts of 180 whole chicken carcasses collected in 6 supermarkets (SM), 6 indoor markets (IM), and 6 open markets (OM) in Vietnam, at opening (T0) and 4 h after the opening (T4). Salmonella and Listeria prevalence were at least 30.4 and 56.6%, respectively. Chicken carcasses had more than 10.1, 7.5, and 9.4 log CFU/g of aerobic bacteria, Escherichia coli(E. coli), and coliforms, respectively. Both E. coli and coliform counts were greater in IM than in SM (P = 0.002 and 0.006). However, only E. coli counts differed between SM (7.7 log CFU/g) and OM (8.3 log CFU/g; P = 0.024). Whole birds in IM had greater Salmonellaprevalence than birds from both SM and OM by 28.4 and 23.0% (P = 0.006 and 0.022, respectively). Listeria prevalence was less in SM, at 56.6%, than in IM and OM (78.6 and 73.2%, P = 0.024 and 0.089, respectively). These results highlighted high levels of bacteria and high incidence of Salmonella and Listeria in whole chicken in retail establishments in Vietnam, posing potential food safety and public health risks.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"48 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78045860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abstracts from the 2021 AMSA Reciprocal Meat Conference","authors":"Deidrea Mabry","doi":"10.22175/mmb.13203","DOIUrl":"https://doi.org/10.22175/mmb.13203","url":null,"abstract":"Abstracts from the 2021 AMSA Reciprocal Meat Conference","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"2010 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82595464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of consumer response using paper and digital ballots for eating quality assessment of beef steaks","authors":"K. T. Mahagan, A. Garmyn, J. Legako, Mark F. Miller","doi":"10.22175/mmb.12611","DOIUrl":"https://doi.org/10.22175/mmb.12611","url":null,"abstract":"A consumer study was conducted to determine if consumers scored beef palatability traits differently on paper vs. digital ballots. Beef subprimals were collected to represent four treatments with inherent variation in eating quality: USDA Select eye round aged 7 d postmortem, USDA Select strip loin aged 7 d postmortem, USDA Choice tenderloin aged 21 d postmortem, and USDA Prime strip loin aged 21 d postmortem. Accessory muscles, external fat, and connective tissue were removed from subprimals. Muscles were fabricated into 2.5 cm steaks and further divided into 2 equal halves for consumer testing. Consumers (n = 360) were served eight samples divided into two blocks representing the two ballot types. Select longissimus lumborum samples were always served in the first and fifth position to start each ballot block, with the remaining three treatments served in a randomized order between the latter three positions within each ballot block. Consumers rated each steak sample for tenderness, juiciness, flavor liking, and overall liking on either a paper or digital ballot, and then rated the paired steak halves on the opposite ballot during the second block of sample testing. Ballot type influenced (P < 0.02) all traits, as consumers scored tenderness, juiciness, flavor liking, and overall liking greater (P < 0.05) on paper ballots compared to digital ballots, regardless of treatment. The smallest margin between paper and digital ballots was observed for tenderness, where scores only differed by 1.8 units. However, juiciness, flavor liking, and overall liking all differed by 3.4 units in favor of paper ballots. Consumers scored palatability traits greater on paper ballots compared to digital ballots, and these findings were consistent across a range of eating quality outcomes. These results should be considered when comparing past consumer testing results using paper balloting systems to those where digital ballots were used.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73019189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2018 National Beef Flavor Audit: Consumer and Descriptive Sensory Attributes","authors":"R. Miller, Cassandra A. Pena, C. Kerth, Paige Smith, K. Wall, J. Legako, T. Cramer, D. Woerner, J. Brooks, M. Nair, Bailey Schilling","doi":"10.22175/mmb.13017","DOIUrl":"https://doi.org/10.22175/mmb.13017","url":null,"abstract":"Beef flavor has been identified as a driver of consumer acceptability; however,  little is known about variability of flavor in major retail beef cuts. Four beef cuts (chuck roast = 50, top sirloin steaks = 49, top loin steaks =50, and 80/20 ground beef = 50) were obtained from retail stores in Miami, Los Angeles, Portland, New York, and Denver during a two-month period in 2018. Beef present in the retail beef cases were purchased to be representative of consumer selections.  Production systems or package claims were documented.  Chuck roasts were oven roasted and top loin, top sirloin and ground beef (made into patties) were grilled to an internal temperature of 71˚C. An expert, trained flavor and texture descriptive attribute sensory panel evaluated beef flavors, aromas and textures and consumer sensory panels in Fort Collins CO and Lubbock TX evaluated beef for overall, overall flavor, beef flavor, grilled flavor, juiciness and texture liking.  Ground beef was more intense (P <0.0001) in brown, fat-like, green hay, and sour milk/sour dairy flavor aromatics; and salty and sweet basic taste than steak cuts. Additionally, ground beef patties had the lowest levels (P<0.0001) of bloody/serumy, metallic, and liver-like flavor aromatics. Chuck roasts had the lowest levels of (P<0.0001) beef flavor identity, brown, and roasted flavor aromatics, and salt and umami basic tastes. Sirloin steaks were lowest (P<0.0001) in fat-like flavor aromatics and most intense (P<0.0001) in burnt and cardboardy flavor aromatics; and bitter and sour basic tastes. Sirloin steaks and chuck roasts were more intense in metallic and liver-like (P<0.0001) flavor aromatics. Ground beef patties had a higher incidence of green hay-like.  Consumers rated chuck roasts lowest for overall, overall flavor, grilled flavor and juiciness liking (P<0.04).  Ground beef pattes and top loin steaks had the highest consumer texture liking (P<0.0002). Therefore, variation in beef flavor attributes were identified in retail beef cuts and ground beef.  Beef descriptive flavor and texture attributes were related to consumer liking and negative flavor aromatic attributes were identified.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90943157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spices, Seasonings and Flavors - Achieving the Desired Result","authors":"J. W. Lamkey","doi":"10.22175/mmb.13016","DOIUrl":"https://doi.org/10.22175/mmb.13016","url":null,"abstract":"Creating a meat entree that provides a pleasant gastronomic experience is a combination of art, science and technology.  Spices and flavorings are used to achieve that experience, each contributing to the overall taste that is often unique to the region of its origin.  Spices have a very colorful history, at one time costing the lives of brave individuals who sacrifice all to bring some of the bounty home and open new routes for future commerce.  Flavorings have the ability to be tailor made and offer the unique ability to provide a taste that cannot be easily developed through normal preparation processes.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73865450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repackaging Nitrite-embedded Dark-Cutting Steak in Aerobic Polyvinyl Chloride Film Decrease Surface Redness","authors":"M. Denzer, G. Mafi, D. VanOverebeke, R. Ramanathan","doi":"10.22175/mmb.12944","DOIUrl":"https://doi.org/10.22175/mmb.12944","url":null,"abstract":"Theoverall goal was to evaluate the effects of repackaging nitrite-embeddeddark-cutting steaks in polyvinyl chloride (PVC) film on surface color.Dark-cutting beef strip loins (n = 8; pH = 6.39) and USDA Low Choice beef striploins (normal-pH, n = 6) were selected at a commercial packing plant.Dark-cutting loins were bisected and randomly assigned to nonenhanceddark-cutting and enhanced dark-cutting with glucono delta-lactone and rosemarytreatments. Normal-pH and nonenhanced dark-cutting steaks were vacuum packaged (VP)and served as controls, while enhanced dark-cutting steaks were packed innitrite-embedded packaging (NP). Steaks from nonenhanced normal-pH in VP, non-enhanceddark-cutting-VP, and enhanced dark-cutting-NP loins were randomly assigned to3, 6, or 9 d of dark storage. Following dark storage, steaks were repackaged inPVC and displayed for 6 d. The instrumental color, visual color, and aerobicplate count were evaluated for all steaks.  Enhanced dark-cutting steaks in nitritepackaging increased (P < 0.05) a* values and nitric oxide myoglobincontent during 24 h of dark storage. Enhanced dark-cutting steaks packaged inNP had greater a* and L* values (P < 0.05) than nonenhanced dark-cutting-VP steaks during darkstorage. Upon repackaging the enhanced dark-cutting steaks from nitritepackaging, nitric oxide myoglobin decreased (P < 0.05) during the first 12 h of display. Loss of nitric oxidemyoglobin corresponds with a darker red appearance, increased surfacediscoloration, and decreased a*values. There were no differences (P> 0.05) in aerobic plate count between enhanced dark-cutting-NP and non-enhanceddark cutting VP steaks during 9 days of dark storage. In conclusion, NPimproved surface redness; however, repackaging enhanced dark-cutting from NP steaksin PVC decreased color stability and redness of dark-cutting beef. ","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"12 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78477506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}