Cryopreservation Biotechnology in Biomedical and Biological Sciences最新文献

Cryopreservation Protocols for Grapevine Shoot Tips 葡萄藤茎尖的低温保存方法

Cryopreservation Biotechnology in Biomedical and Biological Sciences Pub Date : 2018-12-12 DOI: 10.5772/INTECHOPEN.81802

Z. Marković, D. Preiner, D. Stupić, ŽeljkoAndabaka, I. Šikuten, J. K. Kontić, E. Maletić, Petra Štambuk

引用次数: 1

Cryopreservation of Platelets: Advances and Current Practice 血小板的低温保存:进展和当前实践

Cryopreservation Biotechnology in Biomedical and Biological Sciences Pub Date : 2018-12-07 DOI: 10.5772/INTECHOPEN.81906

M. Bohoněk

{"title":"Cryopreservation of Platelets: Advances and Current Practice","authors":"M. Bohoněk","doi":"10.5772/INTECHOPEN.81906","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.81906","url":null,"abstract":"Conventional fresh platelets stored at 20–24°C have a short shelf life, at most 7 days. Their main disadvantage is logistics as it is more difficult. This limitation is especially problem atic for emergency and intensive care departments for managing massive bleeding. The early and aggressive use of blood products for massive hemorrhage may correct coagu -lopathy, control bleeding, and improve outcomes. The timely availability of platelets at the shortest time after the injury is often problematic. Many hospitals cannot afford to have platelets permanently in stock because of its short shelf life. Cryopreservation and storage of frozen platelets may significantly prolong their shelf life. Thus, frozen platelets provide long-term accessibility in situations where fresh products are not available. The most widely used method for the platelets cryopreservation is freezing at 5–6% DMSO at −80°C. The production of cryopreserved platelets is not technologically demanding, they can be easily thawed and reconstituted. Frozen platelets are an alternative blood product for urgent orders in connection with heavy bleeding. They are cost-effective functional platelets product for the management of bleeding and should be considered for wider use in clinical practice, such as autologous platelets, rare or HLA/HPA compatible plate - lets and platelets for non-transfusion use.","PeriodicalId":182510,"journal":{"name":"Cryopreservation Biotechnology in Biomedical and Biological Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126384375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Cryopretectants and Their Usage in Cryopreservation Process 低温保护剂及其在低温保存中的应用

Cryopreservation Biotechnology in Biomedical and Biological Sciences Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.80477

Sankha Bhattacharya

{"title":"Cryopretectants and Their Usage in Cryopreservation Process","authors":"Sankha Bhattacharya","doi":"10.5772/INTECHOPEN.80477","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.80477","url":null,"abstract":"Cryoprotectants are basically some chemical compounds which prevent cells or tissues from damage due to freezing. Mostly vitrification and thawing process are mostly used in cryopreservation. Now a day’s cryopreservation is becoming an apex technology for Health sector, as many organ and tissue transplant methods has been developed in recent time. Organs or tissues need to preserve properly before implantation, hence cryoprotectants plays a key role modern cryonics. This article seeks to provide answers for how cryoprotectants works and prevent tissue damage from shrinking ice crystals during freeze drying or vitrification. The various animal tissues, organs, lymphocytes, cartilages, bone marrow, proteins can be stored by using proper cryoprotectants. Optimization of cryoprotectants is a challenging task, as elevated concentration may cause cytotoxicity. This article also highlights the implication, challenges and recent advances of cryoprotectants along with its types. The importance of cryobank system and its importance were also being emphasized in this article. Further, importance of cryoprotectants in nano suspension preparation was also been discussed in this article.","PeriodicalId":182510,"journal":{"name":"Cryopreservation Biotechnology in Biomedical and Biological Sciences","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127848226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Biological Signals of Sperm Membrane Resistance to Cryoinjury in Boars 公猪精子膜抗低温损伤的生物学信号

Cryopreservation Biotechnology in Biomedical and Biological Sciences Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.80824

J. Valencia, F. J. Henao

引用次数: 2

Introductory Chapter: Application Fields of Cryopreservation Biotechnology 导论:低温保存技术的应用领域

Cryopreservation Biotechnology in Biomedical and Biological Sciences Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.82229

Yusuf Bozkurt

引用次数: 4

Clinical Outcomes of Assisted Reproductive Techniques Using Cryopreserved Gametes and Embryos in Human Medicine 人类医学中使用冷冻配子和胚胎辅助生殖技术的临床结果

Cryopreservation Biotechnology in Biomedical and Biological Sciences Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.80627

Max Waterstone, A. Anastácio, K. Rodriguez-Wallberg

引用次数: 1