Cryopreservation Protocols for Grapevine Shoot Tips

Abstract

Grapevine is an important plant species known worldwide, counting more than 10,000 cultivars of Vitis vinifera spread all over the world. There is a strong need for long-term conservation of grapevine genetic resources. With so polymorphic species, it is highly difficult to obtain relevant results considering that cryopreservation protocols were established testing only few very often specific cultivars. Regarding cryopreservation protocols, many factors are influencing the final result. Research articles processing cryo preservation protocols of grapevine are reporting a percentage of recovery, but a broader application of some protocol on a large range of Vitis vinifera cultivars is limited. How to design an efficient cryopreservation protocol, starting from plant material to the appro - priate observation of recovery, will be discussed in this chapter.