Marine environmental research最新文献

{"title":"Corrigendum to “Quarry rock reef design features influence fish assemblage structure across a systematically heterogenous restoration reef” [Mar. Environ. Res. 202 (2024) 106773]","authors":"James W. Sturges , Jeremy T. Claisse","doi":"10.1016/j.marenvres.2024.106867","DOIUrl":"10.1016/j.marenvres.2024.106867","url":null,"abstract":"","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"205 ","pages":"Article 106867"},"PeriodicalIF":3.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic and phenotypic effects of ocean acidification on cuttlefish differ across early life stages","authors":"Antoine Minet ,&nbsp;Steven Melvin ,&nbsp;Marc Metian ,&nbsp;Angus Taylor ,&nbsp;François Oberhänsli ,&nbsp;Christel Lefrançois ,&nbsp;Peter Swarzenski ,&nbsp;Paco Bustamante ,&nbsp;Thomas Lacoue-Labarthe","doi":"10.1016/j.marenvres.2025.107013","DOIUrl":"10.1016/j.marenvres.2025.107013","url":null,"abstract":"<div><div>Ocean acidification (OA) affects the physiology and behaviour of some marine organisms, impacting their development and metabolism during vulnerable early-life stages. Among them, the embryo of the cuttlefish develops for about two months in encapsulated eggs with harsh perivitelline conditions of hypoxia and hypercapnia, potentially worsened by OA. In this study, common cuttlefish <em>Sepia officinalis</em> embryos and juveniles<em>,</em> were exposed to five pH conditions (pH_T 8.08 to 7.43). Growth, development and metabolite profiles were explored during the embryonic development period up to 10 days-post-hatching. Our results show delayed embryonic development and decreased hatching success at pH 7.43, but no effect on juvenile weight upon hatching. The ¹H Nuclear Magnetic Resonance (NMR) spectroscopy revealed that decreasing pH affected metabolites profiles in embryos until a metabolic suppression was observed at pH 7.43. The O₂ consumption in 10d-old juveniles did not change with pH whereas metabolites indicated a switch to anaerobic metabolism under low pH. Overall, our results suggest that the transition from the encapsulated embryonic stage to the free juvenile life shapes a metabolomic reprogramming more drastically than ocean acidification.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"205 ","pages":"Article 107013"},"PeriodicalIF":3.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and risks of microplastics and phthalate esters in the transition from inland river systems to estuarine and nearshore regions of the Yellow Sea, China","authors":"Lei Chen,&nbsp;Shiyu Fu,&nbsp;Xinyi Zheng","doi":"10.1016/j.marenvres.2025.107029","DOIUrl":"10.1016/j.marenvres.2025.107029","url":null,"abstract":"<div><div>Microplastics (MPs) and phthalate esters (PAEs) are emerging pollutants of significant environmental and health concern. The Yellow Sea, a semi-enclosed marginal sea with dense coastal populations and industrial activities, serves as a critical region for studying MP and PAE pollution due to its ecological sensitivity, role in pollutant transport, and relevance to global marine pollution challenges. The distribution and characteristics of MPs and PAEs in surface water and sediment transitioning from an inland river system to estuarine and nearshore regions of the Yellow Sea in China were investigated. MP concentrations in water samples ranged from 0.89 ± 0.15 to 11.47 ± 1.80 items/L and in sediments from 93.33 ± 23.09 to 653.33 ± 50.33 items/kg dw. The main colors of MPs found in water and sediment samples were white and transparent, with fibers being the predominant shape. The primary size range was 0–0.5 mm, and the main polymer components were rayon and polyethylene. The characteristics of MPs in clams were similar to those in water and sediment, except that their predominant colors were black and blue. The total of six PAEs (Σ₆ PAEs) was detected at concentrations between 0.30 and 1.29 μg/L in water and 25.75–163.61 ng/g in sediments. The concentrations of both pollutants demonstrated a distinct spatial gradient, with the highest levels observed in upstream urban areas, followed by progressively decreasing levels in downstream rural zones, and reaching their minimum concentrations in nearshore regions. Variations in the morphological characteristics (color, shape, and size) and polymer composition of MPs were observed between the aquatic phase and sediment phase along the direction of water flow. A significant correlation was found between MP abundance and Σ6 PAEs across both matrices. Ecological risk assessments revealed substantial risks associated with the presence of these pollutants, particularly in urban areas where contamination peaked. Clams collected from the nearshore regions exhibited MP counts of 1.91 ± 0.47 to 2.49 ± 0.63 items/individual and PAEs from 0.51 to 0.91 μg/g, posing high polymer risk from MPs yet no significant health risk from PAEs for human consumers. This study underscores the transition of MP and PAE pollution from riverine to marine environments, providing valuable insights into the critical sources and potential risks associated with marine MPs and PAEs.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"205 ","pages":"Article 107029"},"PeriodicalIF":3.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What's on the menu? Diet of common minke whales (Balaenoptera acutorostrata) stranded on the Atlantic Iberian coast","authors":"Silvia S. Monteiro ,&nbsp;Andreia Torres-Pereira ,&nbsp;Marisa Ferreira ,&nbsp;José V. Vingada ,&nbsp;Lídia Nicolau ,&nbsp;Marina Sequeira ,&nbsp;Alfredo López ,&nbsp;Pablo Covelo ,&nbsp;Maria Inês Azevedo ,&nbsp;Gema Hernandez-Milian ,&nbsp;Graham J. Pierce ,&nbsp;Catarina Eira","doi":"10.1016/j.marenvres.2025.107024","DOIUrl":"10.1016/j.marenvres.2025.107024","url":null,"abstract":"<div><div>There is little information regarding the ecology of common minke whales (<em>Balaenoptera acutorostrata</em>) in southern waters of the Northeast Atlantic. As such, stomach contents from common minke whales stranded in the west Iberian Peninsula were analysed (n = 27, 2005–2024) to improve the knowledge about the foraging ecology of this species. Overall, small pelagic fish species (<em>Sardina pilchardus</em>, <em>Trachurus</em> sp., <em>Scomber</em> sp.), that are also targeted by fisheries in this region, were predominant in the diet of common minke whales. In particular, <em>Sardina pilchardus</em> and <em>Trachurus</em> sp. were predominant in Portugal (n = 20; %N: 39.4 and 18.2; %W: 32.9 and 27.9; %IRI: 52.2 and 22.2 respectively), while <em>Scomber</em> sp. prevailed in Northwest Spain (Galicia) (n = 7; %N: 28.7; %W: 53.1; %IRI: 48.8), closely followed by <em>Sardina pilchardus</em> (%N: 40.9; %W: 34.6; %IRI: 45). Despite the similarity in the predominant species (Alosidae and Scombridae families) between sexes, females showed a less diverse diet compared to males. Results suggest a temporal shift in the diet of common minke whales in the west Iberian Peninsula (2005–2015 vs. 2016–2024), possibly related with changes in sardine abundance in this region, resulting from prolonged poor recruitment over several years associated with fishing mortality above sustainable levels. In addition, the estimated length of the main prey ingested by common minke whales was within the legal landing size allowed for fisheries in the west Iberian Peninsula. Finally, both minke whales and fisheries target the same energy-rich species, required to meet the high metabolic cost of living of minke whales. As such, the results of this study suggest the potential for minke whale-fisheries resource competition (in terms of species, size class and quality), stressing the potential indirect or direct threat that this socioeconomic activity may represent for common minke whales. This information may be relevant for the definition of efficient conservation strategies for this Vulnerable population. This study presents the first description of the diet composition of common minke whales in the southern part of their distribution range, in the North Atlantic.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"205 ","pages":"Article 107024"},"PeriodicalIF":3.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial and temporal variations in coral reef recovery amid recurring bleaching events in Okinawa Island, Japan","authors":"Mariyam Shidha Afzal ,&nbsp;Junichiro Ishida ,&nbsp;Rickdane Gomez ,&nbsp;Tatsuya Udo ,&nbsp;Meangeldil Ayla Azuma-Malsol ,&nbsp;Lucas Yutaka Kimura ,&nbsp;Takashi Nakamura","doi":"10.1016/j.marenvres.2025.107033","DOIUrl":"10.1016/j.marenvres.2025.107033","url":null,"abstract":"<div><div>Climate change is altering coral reefs around the world at an unprecedented rate, with increasing frequency and magnitude of mass bleaching events caused by high sea surface temperatures (SSTs). In this study, we examined coral communities through reef transect surveys at two depth zones (1–5m and 7–12m) across 11 sites on Okinawa Island, Japan. This study focused on trends in coral cover and composition in Okinawa Island, particularly following the thermal stress events of 2017 and 2022 and the high SSTs of 2023. We assessed coral community composition and bleaching incidence across coral genera, sites, years and depths. In 2017, the overall bleaching percentage was 33.28%, whereas by 2022, bleaching incidence was 9.47%. Sensitivity to bleaching varied among coral genera, with those typically exhibiting massive to encrusting growth forms, such as <em>Porites, Montipora, Goniastrea, Favites,</em> and <em>Platygyra,</em> being more impacted compared to branching and tabular species. Bleaching incidence also differed between depth zones, with 36% overall bleached in shallow depths and 27% in deeper depths in 2017. Despite recurrent bleaching events, hard coral cover across Okinawa Island increased from 13.85% in 2017 to 28.47% by 2023, demonstrating recovery potential despite recurrent bleaching events. Our findings indicated that recurrent bleaching events could alter the bleaching susceptibility and abundance of key coral genera over spatial and temporal scales. Considering the current state of the Okinawan reefs, developing and implementing effective management and protection strategies remains critical to reduce the impacts from global and local stressors.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"205 ","pages":"Article 107033"},"PeriodicalIF":3.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unprecedented warming impacts on phytoplankton and special emphasis on diatom-diazotroph associations in the oligotrophic waters of the Eastern Arabian sea","authors":"Chazhikulam Rajan Vishal ,&nbsp;Manguesh Uttam Gauns ,&nbsp;Anil Kiran Pratihary ,&nbsp;Balamurugan Sadaiappan","doi":"10.1016/j.marenvres.2025.107038","DOIUrl":"10.1016/j.marenvres.2025.107038","url":null,"abstract":"<div><div>The impact of anomalous warming on the phytoplankton dynamics in the euphotic zone (0–60 m depth) of the eastern Arabian Sea (EAS) during the southwest monsoon (SWM–2020) was investigated along the 68°E transect from 8°N to 21°N. During SWM–2020, sea surface temperatures (SSTs) along the transect exceeded 28 °C, with temperatures of ∼29–30 °C recorded in the North of the Findlater Jet Axis (NFJA). The active Findlater Jet (FJ) modulated the thermocline, causing shallower thermocline in the North EAS (NEAS, 16–21°N) and deeper thermocline in the South EAS (SEAS, 8–15°N). The increased stratification due to warming reduced the vertical mixing and exacerbated the oligotrophic condition within the MLDs in the NEAS. High dissolved inorganic silicate and phosphorous with low nitrate concentrations favouring dinoflagellates and diatom-diazotroph associations (DDAs) within the MLDs, particularly in the NEAS. In the NEAS, heterocystous cyanobacteria <em>Richelia</em> was abundant as free-living and in endosymbiosis with <em>Rhizosolenia</em> and <em>Hemiaulus</em>, while coccoid cells of <em>Crocosphaera,</em> symbiotic with <em>Climacodium</em> sp., thrived in both SEAS and NEAS indicating a critical role of dissolved iron in distribution of DDAs. DDAs were abundant in subsurface waters (25–40 m) and rare or absent within the nutricline and subsurface chlorophyll maximum (SCM, 40–60 m depths). High dissolved ammonium concentration in the NEAS indicated that N₂ fixed by DDAs could be supplied into N-limited waters. A high abundance of unhealthy and moribund host-symbiotic stages suggests extreme warming, and shallower nitracline could harm DDAs in the EAS. This study highlights the importance of DDAs in the EAS, which remains poorly understood, and whose underestimation in N₂ and C fixation can impact the regional nitrogen and carbon budgets.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"207 ","pages":"Article 107038"},"PeriodicalIF":3.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking N2O respiratory pathways in Stutzerimonas stutzeri PRE-2: Implications for reducing N2O emissions from estuaries","authors":"Baoshan Zhang,&nbsp;Jiaxian Zhou,&nbsp;Jiapeng Wu,&nbsp;Yu Wang,&nbsp;Fei Ye,&nbsp;Xiaomei Shen,&nbsp;Yiguo Hong","doi":"10.1016/j.marenvres.2025.107044","DOIUrl":"10.1016/j.marenvres.2025.107044","url":null,"abstract":"<div><div>Although nitrogen cycling in nitrogen-enriched estuaries is highly active, the reported nitrous oxide (N₂O) emission factor (EF_5e) values for N₂O emissions in estuarine environments are usually low. Therefore, biological or abiotic mechanisms control the emission of N₂O from estuarine ecosystems. In this study, a pure culture of N₂O-reducing bacteria was isolated from Pearl River Estuary surface sediment and identified as <em>Stutzerimonas stutzeri</em> PRE-2. This strain displayed a high N₂O reduction capability, and the average N₂O reduction rate was 17.93 ± 0.43 μmol h⁻¹ under anoxic conditions. This reduction of N₂O was coupled with the stoichiometric consumption of acetate or lactate as electron donors, suggesting that microbial N₂O reduction involves electron transport. Furthermore, N₂O reduction can yield energy that supports microbial growth<em>.</em> Genomic analysis demonstrated that the strain <em>Stutzerimonas stutzeri</em> PRE-2 contains a complete pathway for the reduction of N₂O to N₂. Typical respiratory chain inhibitors did not significantly inhibit N₂O reduction activity, demonstrating that the electron transfer pathway involved in N₂O reduction is unique compared to the classic respiratory chain. The integrated evidence suggests that microbial N₂O reduction by <em>Stutzerimonas stutzeri</em> PRE-2 involves N₂O respiration and may play an important role in reducing N₂O emissions in estuarine ecosystems.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"206 ","pages":"Article 107044"},"PeriodicalIF":3.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporal distribution and potential ecological risks of current-use pesticides (CUPs) in Laizhou Bay, China","authors":"Yufei Dai ,&nbsp;Xianli Song ,&nbsp;Jing Zhao ,&nbsp;Limin Wang ,&nbsp;Xiaoru Cui ,&nbsp;Li Lu ,&nbsp;Jingyu Zhang ,&nbsp;Haoyu Zhang ,&nbsp;Dahai Zhang ,&nbsp;Keqiang Li","doi":"10.1016/j.marenvres.2025.107042","DOIUrl":"10.1016/j.marenvres.2025.107042","url":null,"abstract":"<div><div>Pesticides occur widely in coastal waters and potentially pose a risk to marine ecosystems and human health. The spatiotemporal distribution of current-use pesticides (CUPs) was studied in Laizhou Bay (LZB) and the Xiaoqing River watershed in China during the summer of 2022 and spring of 2023. The pesticide composite index (<em>PCI</em>) and persistence index (<em>PI</em>) were established in association with the risk quotient (<em>RQ</em>) to assess the ecological risk in LZB. In this study, 17 and 12 kinds of CUP were detected in rivers and LZB seawater, respectively, with atrazine and neonicotinoid insecticides (NEOs) being the most frequently detected CUPs at higher concentrations. The average concentration of CUPs decreased from rivers to the coast and towards the center of the LZB, with higher levels in spring and lower levels in summer in rivers and a reversed pattern in seawater. The high-risk areas lie in the LZB coastal area and the estuary. Atrazine, chlorpyrifos, and imidacloprid might pose a high risk to aquatic organisms based on the <em>RQ</em> in the LZB, whereas the priority concern pesticides are atrazine and triazophos based on the <em>PCI</em> and <em>PI</em>. This study is of great significance for assessing the composite ecological risk of CUPs in aquatic environments.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"206 ","pages":"Article 107042"},"PeriodicalIF":3.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytoplankton responses to reduced freshwater inflow and acid conditions in a Ramsar estuarine lake","authors":"Chuene P. Lakane ,&nbsp;Janine B. Adams ,&nbsp;Daniel A. Lemley","doi":"10.1016/j.marenvres.2025.107043","DOIUrl":"10.1016/j.marenvres.2025.107043","url":null,"abstract":"<div><div>Global change pressures influence estuarine functionality, driving structural shifts that threaten ecosystem resilience. Estuarine lakes are inherently susceptible to these pressures given their tendency to experience prolonged periods of water residency and mouth closure. As such, this study aimed to investigate water quality and phytoplankton responses to drastic shifts in abiotic conditions during the transition from a dry to wet cycle in the Verlorenvlei Estuarine Lake, South Africa. Initially, drought- and human-induced hydrological constraints resulted in extreme environmental conditions, characterised by low water levels and acidification. These low water levels reduced connectivity, resulting in hypersaline conditions and phytoplankton blooms (&gt;20 μg Chl-<em>a</em> L⁻¹) in the lower reaches, while the main basin became brackish, highly acidic (pH &lt; 4), and ammonium-rich (&gt;10 mg/L). Conversely, increased flow improved connectivity, although the lower reaches remained hypersaline and pH in the main basin remained low. When the mouth was open, conditions became homogenous and oligohaline, with neutralised pH levels (&gt;6.5) and low phytoplankton biomass (&lt;5 μg Chl-<em>a</em> L⁻¹). Upon mouth closure, the lower reaches shifted to mesohaline conditions, the main basin became acidic (pH &lt; 5) and supported high-biomass (&gt;60 μg Chl-<em>a</em> l⁻¹) bloom conditions of non-siliceous taxa, and the upper reaches became disconnected. As such, it is critical for catchment-scale interventions to be implemented that conserve and restore freshwater inflow to dilute acidity and replenish the buffering capacity of the system. Without intervention, ongoing anthropogenic and climate change stressors may push the system towards an irreversible ecological tipping point.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"206 ","pages":"Article 107043"},"PeriodicalIF":3.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodiversity monitoring in remote marine environments: Advancing environmental DNA/RNA sampling workflows","authors":"Michelle Scriver ,&nbsp;Anastasija Zaiko ,&nbsp;Xavier Pochon ,&nbsp;Jo-Ann L. Stanton ,&nbsp;Olga Belonovich ,&nbsp;Gert-Jan Jeunen ,&nbsp;Austen C. Thomas ,&nbsp;Neil J. Gemmell ,&nbsp;Ulla von Ammon","doi":"10.1016/j.marenvres.2025.107041","DOIUrl":"10.1016/j.marenvres.2025.107041","url":null,"abstract":"<div><div>Understanding biodiversity is crucial for protecting unique environments, but acquiring this knowledge is challenging in isolated areas due to limited availability of easy-to-implement biomonitoring tools. To determine optimal sampling strategies in remote regions, environmental DNA and RNA (eDNA and eRNA) sampling workflows were evaluated at 12 sites in three fiords within Fiordland National Park, Aotearoa-New Zealand. For filtration comparison, a modified cruising speed net was used to concentrate eDNA/eRNA onto 20 μm nylon filters, while water from the net's cod-end was filtered through a 5 μm Smith-Root self-preserving filter using the eDNA Citizen Scientist Sampler. To compare preservation methods, Smith-Root filters were cut in half, with one half preserved in the self-preserving unit and the other in DNA/RNA Shield™ buffer. Biodiversity screening was performed by sequencing the 18S rRNA gene for eukaryotes and two mitochondrial 16S rRNA genes for fish and marine vertebrates. Comparable amplicon sequence variant (ASVs) richness was observed between methods, yet samples preserved with buffer showed higher richness of fish and marine vertebrate taxa and higher PCR amplification success. There was little variation in community composition, except for 16S rRNA targeting fish, where distinct patterns emerged based on preservation methods. Overall, sampling workflows showed similar community composition and alpha diversity across both nucleic acids. These results confirm that enhancing eDNA/eRNA yields for sparse taxa requires consideration of collection and preservation methods. However, abundant taxa biodiversity is captured consistently, allowing for adjustments without compromising robustness. These insights support streamlined eDNA/eRNA sampling, emphasizing adaptive strategies based on targeted taxa.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"206 ","pages":"Article 107041"},"PeriodicalIF":3.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}