{"title":"Complementary broad-range mitochondrial markers improve eDNA characterization of marine metazoan diversity","authors":"Zhi Ting Yip, Z. B. Randolph Quek, Danwei Huang","doi":"10.1007/s12526-023-01385-6","DOIUrl":"https://doi.org/10.1007/s12526-023-01385-6","url":null,"abstract":"<p>Environmental DNA (eDNA) metabarcoding leverages genetic material present in the habitat for detection of species and has great potential for rapid biodiversity assessment. Comprehensive eDNA characterization of marine metazoan biodiversity requires broad amplification of genetic markers or complementary loci to detect a wide diversity of taxa. In this study, we tested the effectiveness of the universal cytochrome c oxidase subunit I (COI) and marine vertebrate 16S ribosomal RNA markers to recover coastal marine taxa from eDNA samples collected along the urban coast of Singapore. We recovered 260 molecular operational taxonomic units (MOTUs) from a total of 12 L of water (triplicates of 1 L at each of four sites) with no overlap of MOTUs observed between the two assays despite the expectation that the COI primer set could potentially amplify a broad range of metazoans. Notably, our COI assay identified mainly invertebrates, while the 16S assay primarily recovered vertebrates. For the relatively new 16S barcoding marker, we applied two species delimitation programs on a curated, comprehensive vertebrate dataset to determine the distance threshold between intra- and interspecific comparisons (2%). Community analyses revealed that metazoan communities were distinct between sites for both assays. In addition, DNA metabarcoding was shown to be helpful for the monitoring of non-indigenous and threatened marine species, such as <i>Mytella strigata</i> and <i>Aetobatus ocellatus</i>, respectively. Our findings highlight the potential of a complementary multi-marker approach for biomonitoring invertebrates and vertebrates comprehensively across distinct habitats.</p>","PeriodicalId":18201,"journal":{"name":"Marine Biodiversity","volume":"22 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138541107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Glafira Kolbasova, Vitaly Syomin, Alexey Mishin, Tina Molodtsova, Nikolai Neretin, Tatiana Neretina
{"title":"Holopelagic Annelida from the Atlantic sector of the Southern Ocean and adjacent South Atlantic with descriptions of three new species","authors":"Glafira Kolbasova, Vitaly Syomin, Alexey Mishin, Tina Molodtsova, Nikolai Neretin, Tatiana Neretina","doi":"10.1007/s12526-023-01380-x","DOIUrl":"https://doi.org/10.1007/s12526-023-01380-x","url":null,"abstract":"<p>Pelagic polychaetes were collected in the Bransfield Strait, the Weddell Sea, and near the South Orkney Islands area in the austral summer of 2022 during the 87<sup>th</sup> cruise of the R/V Akademik Mstislav Keldysh. The collected material was studied using morphological, taxonomic, and molecular methods; 18S and D1 regions of 28S genes were used as molecular markers. In total, 17 species were recognized in the studied material, including Alciopini (2), Lopadorrhynchidae (4), Iospilidae (1), Typhloscolecidae (6), and Tomopteridae (4). We clarified relationships between <i>Vanadis antarctica</i> McIntosh, 1885, and <i>V. longissima</i> (Levinsen, 1885). One new typhloscolecid <i>Typhloscolex keldyshi</i> sp. nov. and two new species of Lopadorrhynchidae <i>Pelagobia albertych</i> sp. nov. and <i>Pelagobia torquata</i> sp. nov. have been described. Also, we propose to restore <i>Pelagobia viguieri</i> Gravier, 1911, based on molecular data.</p>","PeriodicalId":18201,"journal":{"name":"Marine Biodiversity","volume":"89 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138541121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marco Casartelli, Tullia I. Terraneo, Arthur Anker, Silvia Vimercati, Davide Maggioni, Gustav Paulay, Francesca Benzoni
{"title":"New ecological and phylogenetic insights in the boring barnacle Berndtia Utinomi, 1950 (Acrothoracica: Lithoglyptidae) reveal higher diversity, new hosts, and range extension to the Western Indian Ocean","authors":"Marco Casartelli, Tullia I. Terraneo, Arthur Anker, Silvia Vimercati, Davide Maggioni, Gustav Paulay, Francesca Benzoni","doi":"10.1007/s12526-023-01379-4","DOIUrl":"https://doi.org/10.1007/s12526-023-01379-4","url":null,"abstract":"<p>The acrothoracican genus <i>Berndtia</i> Utinomi, 1950 includes small barnacles known to bore into the calcareous skeleton of living scleractinian corals of the genera <i>Psammocora</i> Dana, 1846 and <i>Leptastrea</i> Milne Edwards & Haime, 1849. The six known species of <i>Berndtia</i> are restricted to the tropical Western Pacific. We provide the first record of <i>Berndtia</i> from the hydrocoral <i>Millepora exaesa</i> Forsskål, 1775 from the central Red Sea, Saudi Arabia, and the scleractinian coral <i>Coscinaraea</i> cf. <i>monile</i> (Forsskål, 1775) from the Arabian Sea, Oman. These findings extend the known range and host use of the genus and raise questions about <i>Berndtia</i>’s host specificity. A molecular analysis of Saudi Arabian and Omani specimens suggests that they belong to two new lineages that may represent new species of <i>Berndtia</i>, each associated with multiple hosts. Further sampling around the Arabian Peninsula and the Western Indian Ocean and exploration of additional potential hosts would provide new insights into the species diversity of the genus.</p>","PeriodicalId":18201,"journal":{"name":"Marine Biodiversity","volume":"26 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138541104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guillermo Mironenko Castelló, Emmeline A. Jamodiong, Jue Alef Avanzado Lalas, Rahul Mehrotra, James Davis Reimer
{"title":"Distribution and molecular phylogeny of the octocoral genus Nanipora (Helioporidae) in the western Pacific","authors":"Guillermo Mironenko Castelló, Emmeline A. Jamodiong, Jue Alef Avanzado Lalas, Rahul Mehrotra, James Davis Reimer","doi":"10.1007/s12526-023-01383-8","DOIUrl":"https://doi.org/10.1007/s12526-023-01383-8","url":null,"abstract":"","PeriodicalId":18201,"journal":{"name":"Marine Biodiversity","volume":"42 9","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136347173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Josie Antonucci Di Cavalho, Lena Rönn, Theo C. Prins, Helmut Hillebrand
{"title":"Temporal change in phytoplankton diversity and functional group composition","authors":"Josie Antonucci Di Cavalho, Lena Rönn, Theo C. Prins, Helmut Hillebrand","doi":"10.1007/s12526-023-01382-9","DOIUrl":"https://doi.org/10.1007/s12526-023-01382-9","url":null,"abstract":"Abstract One of the key challenges in managing eutrophication in coastal marine ecosystems is the harmonized cross-border assessment of phytoplankton. Some general understanding of the consequences of shifting nutrient regimes can be derived from the detailed investigation of the phytoplankton community and its biodiversity. Here, we combined long-term monitoring datasets of German and Dutch coastal stations and amended these with additional information on species biomass. Across the integrated and harmonized dataset, we used multiple biodiversity descriptors to analyse temporal trends in the Wadden Sea phytoplankton. Biodiversity, measured as the number of species (S) and the effective number of species (ENS), has decreased in the Dutch stations over the last 20 years, while biomass has increased, indicating that fewer species are becoming more dominant in the system. However, biodiversity and biomass did not show substantial changes in the German stations. Although there were some differences in trends between countries, shifts in community composition and relative abundance were consistent across stations and time. We emphasise the importance of continuous and harmonized monitoring programmes and multi-metric approaches that can detect changes in the communities that are indicative of changes in the environment.","PeriodicalId":18201,"journal":{"name":"Marine Biodiversity","volume":"132 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135342380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Risso’s dolphin (Grampus griseus) in Russia with note on Xenobalanus globicipitis","authors":"Sergey D. Ryazanov, Tatiana V. Ryazanova","doi":"10.1007/s12526-023-01378-5","DOIUrl":"https://doi.org/10.1007/s12526-023-01378-5","url":null,"abstract":"","PeriodicalId":18201,"journal":{"name":"Marine Biodiversity","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135859017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ann Bucklin, Jennifer M. Questel, Paola G. Batta-Lona, Mary Reid, Alexandra Frenzel, Cecilia Gelfman, Peter H. Wiebe, Robert G. Campbell, Carin J. Ashjian
{"title":"Population genetic diversity and structure of the euphausiids Thysanoessa inermis and T. raschii in the Arctic Ocean: inferences from COI barcodes","authors":"Ann Bucklin, Jennifer M. Questel, Paola G. Batta-Lona, Mary Reid, Alexandra Frenzel, Cecilia Gelfman, Peter H. Wiebe, Robert G. Campbell, Carin J. Ashjian","doi":"10.1007/s12526-023-01371-y","DOIUrl":"https://doi.org/10.1007/s12526-023-01371-y","url":null,"abstract":"","PeriodicalId":18201,"journal":{"name":"Marine Biodiversity","volume":"159 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135307278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José Raúl Morales-Ávila, Mario Jaime-Rivera, Norma Y. Hernández-Saavedra, Ignacio Leyva-Valencia, César A. Salinas-Zavala, Sergio Hernández-Trujillo, Harry W. Palm
{"title":"Insights into the trophic interactions of the endemic shark Cephalurus cephalus: diet composition and first infection records of Anisakis and other nematodes","authors":"José Raúl Morales-Ávila, Mario Jaime-Rivera, Norma Y. Hernández-Saavedra, Ignacio Leyva-Valencia, César A. Salinas-Zavala, Sergio Hernández-Trujillo, Harry W. Palm","doi":"10.1007/s12526-023-01375-8","DOIUrl":"https://doi.org/10.1007/s12526-023-01375-8","url":null,"abstract":"","PeriodicalId":18201,"journal":{"name":"Marine Biodiversity","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135878320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Evelyn S. M. Fonseca, B. Cóndor-Luján, F. F. Cavalcanti
{"title":"Calcareous sponges (Porifera, Calcarea) from the Northeastern Brazilian coast: integrative taxonomy and support for the creation of a marine park","authors":"Evelyn S. M. Fonseca, B. Cóndor-Luján, F. F. Cavalcanti","doi":"10.1007/s12526-023-01364-x","DOIUrl":"https://doi.org/10.1007/s12526-023-01364-x","url":null,"abstract":"","PeriodicalId":18201,"journal":{"name":"Marine Biodiversity","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42153660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}