Liver Pub Date : 1995-02-01 DOI: 10.1111/j.1600-0676.1995.tb00098.x

W F Seifert, A Bosma, H F Hendriks, R E van Leeuwen, G C van Thiel-de Ruiter, I Seifert-Bock, D L Knook, A Brouwer

{"title":"Beta-carotene (provitamin A) decreases the severity of CCl4-induced hepatic inflammation and fibrosis in rats.","authors":"W F Seifert, A Bosma, H F Hendriks, R E van Leeuwen, G C van Thiel-de Ruiter, I Seifert-Bock, D L Knook, A Brouwer","doi":"10.1111/j.1600-0676.1995.tb00098.x","DOIUrl":"https://doi.org/10.1111/j.1600-0676.1995.tb00098.x","url":null,"abstract":"Earlier data from experiments in rats have shown that administration of retinyl esters (vitamin A) strongly influences the effects of CCl4 on the liver. The accumulation of collagen was inhibited, but an increase in CCl4-toxicity with high mortality was observed. The present study was conducted to examine the effects of beta-carotene (provitamin A) on CCl4-related general and hepatic toxicity in rats. Oral administration of beta-carotene during CCl4-treatment resulted, biochemically, in a significantly lower increase in the hydroxyproline liver content and, histopathologically, in less severe liver fibrosis as compared with the liver of rats not treated with beta-carotene. The study also showed that beta-carotene administration could prevent the long-term loss of retinoids from the CCl4-injured liver. No significant toxic effects of beta-carotene, as previously found with retinyl esters (vitamin A), were observed. This experimental study suggests that beta-carotene has the therapeutic potential to decrease the severity of liver fibrosis without marked toxicity.","PeriodicalId":18183,"journal":{"name":"Liver","volume":"15 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0676.1995.tb00098.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18776651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 61

Protective effect of diltiazem against acetaminophen hepatotoxicity in mice. 地尔硫卓对小鼠对乙酰氨基酚肝毒性的保护作用。

Liver Pub Date : 1995-02-01 DOI: 10.1111/j.1600-0676.1995.tb00100.x

J Satorres, M Pérez-Mateo, M J Mayol, A Esteban, M L Graells

{"title":"Protective effect of diltiazem against acetaminophen hepatotoxicity in mice.","authors":"J Satorres, M Pérez-Mateo, M J Mayol, A Esteban, M L Graells","doi":"10.1111/j.1600-0676.1995.tb00100.x","DOIUrl":"https://doi.org/10.1111/j.1600-0676.1995.tb00100.x","url":null,"abstract":"There is evidence that an increase in cytosolic Ca++ concentration is a terminal event in the progression to cell death in toxic liver injury. We have compared the hepatoprotective effects of N-acetylcysteine (1 g/kg) and the calcium channel blocking agent, diltiazem (24 mg/kg), when given at 30 min, 3 h and 6 h after single intraperitoneal overdoses of acetaminophen (500 mg/kg) in mice. Overall beneficial effects on mortality, liver necrosis score, and plasma alanine aminotransferase (ALT) activity were found in diltiazem-treated mice 24 h after acetaminophen overdose. However, the most marked effects were obtained when diltiazem was given 6 h after acetaminophen. N-acetylcysteine was more effective than diltiazem at 30 min and 3 h, although it was less effective at 6 h. Mean plasma concentrations of the mercapturate metabolite (hepatic oxidative metabolism) were not significantly different among animals receiving acetaminophen alone or in combination with diltiazem, which suggests that the hepatoprotective effects of diltiazem are not exerted by inhibition of drug metabolic enzymes.","PeriodicalId":18183,"journal":{"name":"Liver","volume":"15 1","pages":"16-9"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0676.1995.tb00100.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18776652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

An immunohistochemical analysis of 13 cases with combined hepatocellular and cholangiocellular carcinoma. 13例肝细胞癌与胆管细胞癌合并的免疫组化分析。

Liver Pub Date : 1995-02-01 DOI: 10.1111/j.1600-0676.1995.tb00099.x

J Haratake, H Hashimoto

{"title":"An immunohistochemical analysis of 13 cases with combined hepatocellular and cholangiocellular carcinoma.","authors":"J Haratake, H Hashimoto","doi":"10.1111/j.1600-0676.1995.tb00099.x","DOIUrl":"https://doi.org/10.1111/j.1600-0676.1995.tb00099.x","url":null,"abstract":"Thirteen cases of combined hepatocellular (HCC) and cholangiocellular carcinoma (CCC) were examined. In addition to routine pathology, immunoreactivities for carcinoembryonic antigen, alpha-fetoprotein (AFP), cytokeratin (Cam 5.2 and AE1), epithelial membrane antigen (EMA) and tumor-associated glycoprotein 72 (B72.3) were also examined. The average age of the 13 cases was 64.8 years, which lay between the average ages of pure HCC and CCC cases. They were categorized as separate type (2), collision type (6), and intermingled type (5). AE1 and EMA were the best markers to differentiate the CCC from the HCC area. B72.3 immunoreactivity was detected only in CCC (46%). There were no transitional features between HCC and CCC in two cases of the separate type and two cases of the collision type. However, focal transitional features from HCC to CCC were observed in all cases of the intermingled type and in four of six cases of the collision type. In one case of the intermingled type, many cancer cells contained both bile and mucus simultaneously, and revealed dual immunoreactivities. The conclusions are: 1) the combined type is generated from two sources; one is the intrahepatic double cancer (thoroughly separate type and a part of the collision type) and another is the stem cell origin with diverse phenotypes (intermingled type and a part of the collision tumor); and 2) AE1 was the most helpful marker to differentiate the CCC area from HCC, and other markers, e.g. AFP for HCC and EMA, CEA, and B72.3 for CCC, were also supportive but somewhat limited in the differential diagnosis.","PeriodicalId":18183,"journal":{"name":"Liver","volume":"15 1","pages":"9-15"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0676.1995.tb00099.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18548197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 57

The role of the spleen in endotoxin-induced liver injury. 脾脏在内毒素引起的肝损伤中的作用。

Liver Pub Date : 1995-02-01 DOI: 10.1111/j.1600-0676.1995.tb00104.x

E Hiraoka, T Nonami, T Kurokawa, H Kobayashi, H Takagi

{"title":"The role of the spleen in endotoxin-induced liver injury.","authors":"E Hiraoka, T Nonami, T Kurokawa, H Kobayashi, H Takagi","doi":"10.1111/j.1600-0676.1995.tb00104.x","DOIUrl":"https://doi.org/10.1111/j.1600-0676.1995.tb00104.x","url":null,"abstract":"In these experiments, the role of the spleen in endotoxin-induced liver injury was evaluated, using rats which underwent splenectomy or splenic vein ligation with antecedent spleno-systemic shunt. Male Wistar rats were divided into three groups: a sham-operated group, a splenectomy group, and a splenic vein ligation group. In each animal, 48 h after surgery, 5 mg/kg lipopolysaccharide (LPS) were injected intravenously. Six rats from each group were sacrificed 6 or 12 h after LPS administration. Bronchoalveolar lavage fluid (BALF) and arterial blood were also collected. Splenectomy reduced the liver injury as indicated by the serum lactate dehydrogenase level. A decrease in liver tissue adenosine triphosphate and increase in lipid peroxide were induced by LPS administration and inhibited by splenectomy. Splenectomy also reduced alveolar protein release as indicated by the protein level in BALF. Splenic vein ligation provided similar protective effects on the liver, but did not affect lung injury. From these results, it appears that the spleen plays a significant role in endotoxin-induced liver injury, and a mediator derived from the spleen is likely associated with development of liver injury. This mediator may be cleared or inactivated by not only splenectomy but also splenic vein ligation.","PeriodicalId":18183,"journal":{"name":"Liver","volume":"15 1","pages":"35-8"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0676.1995.tb00104.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18780036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Mannan-binding protein and complement dependent opsonization in alcoholic cirrhosis. 甘露聚糖结合蛋白与补体依赖性调节在酒精性肝硬化中的作用。

Liver Pub Date : 1995-02-01 DOI: 10.1111/j.1600-0676.1995.tb00105.x

C Homann, P Garred, P Hasselqvist, N Graudal, S Thiel, A C Thomsen

{"title":"Mannan-binding protein and complement dependent opsonization in alcoholic cirrhosis.","authors":"C Homann, P Garred, P Hasselqvist, N Graudal, S Thiel, A C Thomsen","doi":"10.1111/j.1600-0676.1995.tb00105.x","DOIUrl":"https://doi.org/10.1111/j.1600-0676.1995.tb00105.x","url":null,"abstract":"Mannan-binding protein is synthesized by the liver and functions in first-line host defence by opsonizing mannose-rich microorganisms due to activation of the classical complement pathway independent of Clq, and by an intrinsic ability to opsonize and mediate phagocytosis. We have investigated whether the increased susceptibility to bacterial infections in patients with cirrhosis could be explained by low plasma concentrations of mannan-binding protein and impaired complement-dependent opsonization. We examined 51 patients with compensated alcoholic cirrhosis, 34 who were decompensated and 16 healthy controls. Irrespective of group, we found a significant correlation (p < 0.05) between plasma mannan-binding protein concentration and deposition of the complement opsonin C4 on mannan from baker's yeast. In contrast to what was expected, this kind of opsonization and plasma levels of mannan-binding protein were significantly increased in the patients with decompensated cirrhosis (p = 0.01 and p = 0.007, respectively). A significant correlation (0 < 0.05) was found between mannan-binding protein and erythrocyte sedimentation rate, fibrinogen and haptoglobin in these patients. Though the correlations were weak (rho = 0.49, rho = 0.48 and rho = 0.40, respectively), the elevated levels of mannan-binding protein in the patients with decompensated cirrhosis may reflect an acute phase reaction. It is concluded that plasma levels of mannan-binding protein are increased in patients with decompensated cirrhosis and that complement-dependent opsonization of mannan does not seem to be compromized in patients with alcoholic cirrhosis.","PeriodicalId":18183,"journal":{"name":"Liver","volume":"15 1","pages":"39-44"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0676.1995.tb00105.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18780037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Macroregenerative nodules in cirrhosis are not associated with elevated serum or stainable tissue alpha-fetoprotein. 肝硬化大再生结节与血清或染色组织甲胎蛋白升高无关。

Liver Pub Date : 1995-02-01 DOI: 10.1111/j.1600-0676.1995.tb00103.x

N D Theise, I M Fiel, P Hytiroglou, L Ferrell, M Schwartz, C Miller, S N Thung

{"title":"Macroregenerative nodules in cirrhosis are not associated with elevated serum or stainable tissue alpha-fetoprotein.","authors":"N D Theise, I M Fiel, P Hytiroglou, L Ferrell, M Schwartz, C Miller, S N Thung","doi":"10.1111/j.1600-0676.1995.tb00103.x","DOIUrl":"https://doi.org/10.1111/j.1600-0676.1995.tb00103.x","url":null,"abstract":"We have explored the relationship of serum alpha-fetoprotein and macroregenerative nodules (MRNs), possible precursor lesions of hepatocellular carcinoma (HCC), and sought to demonstrate alpha-fetoprotein (AFP) expression in these nodules. One hundred and sixty-eight sequential adult cirrhotic resected livers were examined and MRNs were identified by standard criteria. Pretransplant serum AFP was available for 158 of these patients (normal < 20 ng/ml). One hundred and seventy-two randomly selected lesions, including ordinary and atypical MRNs, some containing microfoci of HCC, and HCCs were stained for AFP by immunohistochemistry. In the series, 12 cases had grossly apparent HCCs, four associated with high serum alpha-fetoprotein (p < 0.006). Forty-four cases had MRNs, 32 without grossly apparent HCC. Five of these 32 cases were associated with high serum AFP (not significant). Immuno-staining for AFP was seen in three specimens of HCC and in a cirrhotic nodule from a patient without HCC, but not in MRNs. 1) Neither the presence of MRNs--whether ordinary, atypical, or containing micro-foci of HCC--nor that of gross HCC is ruled out by a normal serum AFP. 2) Elevated serum AFP is not associated with the presence of MRNs. 3) MRNs rarely stain for tissue AFP.","PeriodicalId":18183,"journal":{"name":"Liver","volume":"15 1","pages":"30-4"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0676.1995.tb00103.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18548196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Inapparent "wild-type" and "e-minus variant" HBV infection in patients with HCV-related chronic hepatitis. hcv相关慢性肝炎患者的“野生型”和“e-变体”HBV感染不明显。

Liver Pub Date : 1994-10-01

M A Sardo, G Rodinò, S Brancatelli, M Pernice, S Campo, G Squadrito, F Russo, G Raimondo