Macedonian Veterinary Review最新文献_第2页

Profiling Mycoplasma hyopneumoniae Infection in Commercial Pig Farms Using Serology and Lung Lesions Assessment 利用血清学和肺部病变评估分析商品猪场猪肺炎支原体感染

IF 0.4

Macedonian Veterinary Review Pub Date : 2023-05-06 DOI: 10.2478/macvetrev-2023-0018

Branko Angjelovski, Clara Orenga, Aleksandar Janevski, A. Dodovski, R. Prodanović, J. Bojkovski

{"title":"Profiling Mycoplasma hyopneumoniae Infection in Commercial Pig Farms Using Serology and Lung Lesions Assessment","authors":"Branko Angjelovski, Clara Orenga, Aleksandar Janevski, A. Dodovski, R. Prodanović, J. Bojkovski","doi":"10.2478/macvetrev-2023-0018","DOIUrl":"https://doi.org/10.2478/macvetrev-2023-0018","url":null,"abstract":"Abstract Mycoplasma hyopneumoniae (Mhyo) is primary pathogen involved in porcine respiratory diseases complex (PRDC). The objective of this study was to evaluate Mycoplasma hyopneumoniae (Mhyo) infection in five commercial pig farms by using serology and lung lesion scoring at slaughter. Five Macedonian pig herds with a history of respiratory diseases were included in the study. Blood samples were taken from pigs at 6, 10, 14, 18, and 22 weeks of age. Ten animals per age group from each farm were sampled and tested for antibodies to Mhyo. At abattoir, 50 lungs per farm were scored for lesions associated with enzootic pneumonia (EP). All farms were seropositive to Mhyo. Higher seroprevalence to Mhyo was observed in grower and finisher pigs, while significant difference (p<0.001) was detected among farms in 10-, 14-, and 22-week-old pigs. Enzootic pneumonia-like lesions were detected in 91.2% of all tested lungs with range of 82 to 98% on farm level. Mean lung lesion score (LLS) obtained for all farms was 11.5 (8.04–14.4). Significant difference for LLS and significantly higher percentage of severe LLS grade (>10) were found among some of the farms (p<0.001). In conclusion, high seroprevalence to Mhyo in finishing pigs in most of the farms was most likely due to vaccination. Farms with higher seroprevalence to Mhyo obtained lower LLS. Serology monitoring of different pig categories and lung assessment at slaughterhouse is a practical tool for assessing vaccine efficacy of Mhyo in pig farms.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46847255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-Lactamase Genes (blaCTX-M, blaSHV, blaTEM, blaOXA1 and blaOXA2) and Phylogenetic Groups in ESBL Producing Commensal Escherichia coli Isolated from Faecal Samples from Dairy Farm in the Municipality of Debar 德巴尔市奶牛场粪中产ESBL共生大肠杆菌的β-内酰胺酶基因(blaCTX-M、blaSHV、blaTEM、blaOXA1和blaOXA2)及其系统发育群

IF 0.4

Macedonian Veterinary Review Pub Date : 2023-03-01 DOI: 10.2478/macvetrev-2023-0017

Maksud Kerluku, D. Jankuloski, Marija Ratkova Manovska, M. Prodanov, B. S. Dimzoska, A. Dodovski, K. Blagoevska

{"title":"β-Lactamase Genes (blaCTX-M, blaSHV, blaTEM, blaOXA1 and blaOXA2) and Phylogenetic Groups in ESBL Producing Commensal Escherichia coli Isolated from Faecal Samples from Dairy Farm in the Municipality of Debar","authors":"Maksud Kerluku, D. Jankuloski, Marija Ratkova Manovska, M. Prodanov, B. S. Dimzoska, A. Dodovski, K. Blagoevska","doi":"10.2478/macvetrev-2023-0017","DOIUrl":"https://doi.org/10.2478/macvetrev-2023-0017","url":null,"abstract":"Abstract β-lactamases are a diverse class of enzymes produced by bacteria that present a major cause for resistance to β-lactams. In this study we analysed 159 fecal samples from dairy cows, for the presence of presumptive ESBL, AmpC, and carbapenemase-producing E. coli. Phylotyping was done using Clermont phylo-typing method, targeting arpA, ChuA, and YjaA genes, along with the DNA fragment TspE4.C2. Convetional PCR method was used to confirm the presence of bla genes among 39 phenotypically confirmed ESBL producing E. coli. The results showed presence of CTX-M, SHV, TEM and OXA1 bla genes in 28 (71.79%), 1 (2.56%), 29 (74.35%), 2 (5.12%) of isolates, respectively Twenty (51.28%) isolates showed presence of both blaCTX-M and TEM genes. The strain that carried the blaSHV gene was found to carry blaTEM gene as well, while one of the strains that carried blaOXA1 gene was also carrying blaCTX-M and TEM gene. The ration between isolates and phylo-groups was as follows: 9 (23.07%) strains were assigned to phyllo-group D; 14 (35.89%) to phyllo-group B; 16 (41.02%) to phyllo-group A. Out of the 39 strains where bla genes were identified, 29 (74.35%) were categorized as multi drug resistant.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"46 1","pages":"89 - 97"},"PeriodicalIF":0.4,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44577715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Investigation of Vitamin a and 25(OH)D3 Levels in Cattle with Pneumonia Detected Mycoplasma bovis 牛肺炎支原体检测牛血清维生素a和25（OH）D3水平的研究

IF 0.4

Macedonian Veterinary Review Pub Date : 2023-03-01 DOI: 10.2478/macvetrev-2023-0015

P. Dinçer, Z. Yerlikaya, Burcu Karagülle

{"title":"Investigation of Vitamin a and 25(OH)D3 Levels in Cattle with Pneumonia Detected Mycoplasma bovis","authors":"P. Dinçer, Z. Yerlikaya, Burcu Karagülle","doi":"10.2478/macvetrev-2023-0015","DOIUrl":"https://doi.org/10.2478/macvetrev-2023-0015","url":null,"abstract":"Abstract This study investigated the presence of Mycoplasma bovis in nasal swabs taken from cattle with pneumonia, to reveal the clinical and biochemical findings of positive animals, and determine the levels of vitamin A and 25(OH)D3. In the study were included 103 infected cattle and 10 healthy cattle, all aged 1-4 years, as the control group. Quantitative clinical evaluations and clinical examinations were performed on each animal, and 5 ml blood samples and nasal swabs were taken. Serum biochemistry, vitamin A and 25(OH)D3 levels were determined in both positive and control samples. The clinical signs were identified by qualitative scoring in M. bovis positive animals. Regarding the clinical findings, respiratory frequency, heart rate, and rectal temperature were significantly higher in positive than control group animals (p<0.001). Regarding the biochemistry findings, albumin (ALB) and glucose (GLU) levels were lower in cattle infected with M. bovis (p<0.001) whereas total protein (TP) and alkaline phosphatase (ALP) levels were higher (p<0.001). While serum 25(OH)D3 levels were lower in infected animals (p<0.001), there was no significant difference (p>0.05) in serum vitamin A levels. In this study, clinical findings in cattle naturally infected with M. bovis were expressed qualitatively and quantitatively. In addition, it has been revealed that the disease does not only cause lung involvement, but also causes changes in biochemical and vitamin levels. Although these parameters cannot be used as a biomarker in the diagnosis of pneumonia, they are thought to provide benefits in terms of diagnosis, treatment and prophylaxis in practice.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"46 1","pages":"69 - 77"},"PeriodicalIF":0.4,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46207114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Corrected QT Interval with Bazett’s Method in Cavalier King Charles Spaniel Dogs with Myxomatous Mitral Valve Disease Bazett法对患有粘液瘤性二尖瓣疾病的查尔斯国王犬校正QT间期的评价

IF 0.4

Macedonian Veterinary Review Pub Date : 2023-03-01 DOI: 10.2478/macvetrev-2023-0014

Hande Sağoğlu, R. Gönül, L. Koenhemsi, E. Alan, Suzan Murat, Ashkan Seddigh Nia, M. Or

{"title":"Evaluation of the Corrected QT Interval with Bazett’s Method in Cavalier King Charles Spaniel Dogs with Myxomatous Mitral Valve Disease","authors":"Hande Sağoğlu, R. Gönül, L. Koenhemsi, E. Alan, Suzan Murat, Ashkan Seddigh Nia, M. Or","doi":"10.2478/macvetrev-2023-0014","DOIUrl":"https://doi.org/10.2478/macvetrev-2023-0014","url":null,"abstract":"Abstract Myxomatous mitral valve disease (MMVD) is one of the most common heart diseases in dogs. The disease progresses faster in Cavalier King Charles Spaniel (CKCS) dogs and occurs at an earlier age. QT interval length reflects abnormalities in ventricular repolarization which may predispose to the formation of fatal arrhythmias such as torsades de pointes. A fast and accurate assessment is therefore essential. The study aimed to examine the changes in QT duration in MMVD cases of CKCS and to calculate the corrected QT durations with Bazett’s formula in various stages of the disease. The study included 20 CKCS dogs of both genders, various ages and weights, and different stages of MMVD (n=6 in B1 stage, n=6 in B2 stage, and n=8 in C stage), and 5 healthy CKCS which were included in the control group. Clinical, radiological, hematological, biochemical, echocardiographic, and electrocardiographic examinations were performed. The corrected QT interval duration in the MMVD group was longer than the control (p<0.05). However, there was no significant difference between B1, B2, and C. It was concluded that the corrected QT interval can give a significant distinction between healthy and MMVD CKCS dogs.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"46 1","pages":"61 - 67"},"PeriodicalIF":0.4,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43129210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Potential Serum Biomarkers for the Diagnosis of Chronic Back Pain in Horses 潜在血清生物标志物诊断马慢性背痛的研究

IF 0.4

Macedonian Veterinary Review Pub Date : 2023-03-01 DOI: 10.2478/macvetrev-2023-0016

A. Mayaki, I. S. Razak, N. Adzahan, M. Mazlan, R. Abdullah

{"title":"Investigation of Potential Serum Biomarkers for the Diagnosis of Chronic Back Pain in Horses","authors":"A. Mayaki, I. S. Razak, N. Adzahan, M. Mazlan, R. Abdullah","doi":"10.2478/macvetrev-2023-0016","DOIUrl":"https://doi.org/10.2478/macvetrev-2023-0016","url":null,"abstract":"Abstract Back pain is one of the most common triggers of performance failure in athletic and riding horses. Diagnosis of equine back pain has been very challenging for equine practitioners, particularly in chronic cases. Therefore, the identification of blood biomarkers would facilitate the clinical differentiation of chronic back pain. This study aimed to investigate serum biomarkers of glial cell activation, axonal damage, and inflammation for the diagnosis of equine chronic back pain. Serum samples from forty horses comprising chronic back pain (CBP), back pain concurrent with lameness (BPL), lameness (LN), and healthy control (HC) (n=10 per group) were screened for ionized calcium-binding adaptor molecule 1 (Iba-1), glial fibrillary acidic protein (GFAP), phosphorylated neurofilament-H (pNF-H) by ELISA, and proinflammatory cytokines (IL-1β, IL-6, and TNF-α) by multiplex assay. Serum concentrations of GFAP (3.81±1.72 ng/mL) and pNF-H (0.76±0.18 ng/mL) were significantly (p<0.05) higher in horses with CBP when compared with other groups. Iba-1 was not significantly higher in CBP horses. There was no significant difference between the pro-inflammatory cytokines among the groups. The levels of IL-1β, IL-6, and TNF-α were also increased in the CBP than the HC control horses but lower in relation to BPL and LN horses. In addition, serum Iba-1, GFAP, and pNF-H showed a high discriminatory capacity for horses with CBP with high sensitivity (50-100%) and specificity (70-100%). This study provides evidence that serum levels of the GFAP and pNF-H may be useful in the clinical differentiation of horses with chronic back pain.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"46 1","pages":"79 - 87"},"PeriodicalIF":0.4,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45318507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Modified-Hen-Egg-Yolk-Lipid-Composition Diet on Plasma Lipids in Rats 改良蛋黄脂质复合日粮对大鼠血脂的影响

IF 0.4

Macedonian Veterinary Review Pub Date : 2023-02-27 DOI: 10.2478/macvetrev-2023-0013

A. Hodžić, Husein Ohran, M. Hamamdžić, A. Gagić, A. Hrković-Porobija, E. Pašić-Juhas, A. Selimović, A. Ajanović, J. Krnić

{"title":"Effect of Modified-Hen-Egg-Yolk-Lipid-Composition Diet on Plasma Lipids in Rats","authors":"A. Hodžić, Husein Ohran, M. Hamamdžić, A. Gagić, A. Hrković-Porobija, E. Pašić-Juhas, A. Selimović, A. Ajanović, J. Krnić","doi":"10.2478/macvetrev-2023-0013","DOIUrl":"https://doi.org/10.2478/macvetrev-2023-0013","url":null,"abstract":"Abstract This study aimed to investigate the effects of dietary supplementation with modified-hen-egg-yolk on plasma lipids and lipoprotein profiles in rats. During the four-week-experiment, 64 Wistar rats were divided into four groups of 16 (eight of both sexes), and fed commercial rat food (group C); food containing 70% commercial rat mixture and 30% freshly cooked egg yolk originating from laying hen eggs fed with 3% fish oil (group F); 3% palm olein (group P), or 3% lard (group L). The cooked egg yolk in the rat diet affected the concentrations of plasma total and LDL-cholesterol in males of the P and L groups. Cholesterol and total fat in the diet did not have a hypercholesterolemic effect on their own, but when in combination with fatty acid composition, they could contribute to an increase in plasma total and LDL cholesterol concentrations in rats. HDL-cholesterol was the most resilient plasma lipoprotein of rats to dietary treatments in our experiment. Compared to the control group, the addition of hen egg yolk to the rat diet regardless of its quality, adversely affected the values of HDL-C/TC and HDL-C/LDL-C in both males and females.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"46 1","pages":"51 - 59"},"PeriodicalIF":0.4,"publicationDate":"2023-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48823914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Otitis Externa in Dogs: Distribution and Antimicrobial Susceptibility Patterns of Staphylococcus Spp. Isolates 犬外耳炎:葡萄球菌分离株的分布和对抗生素的敏感性

IF 0.4

Macedonian Veterinary Review Pub Date : 2023-02-07 DOI: 10.2478/macvetrev-2023-0012

Mohammad. H. A. Hassan, A. Kekec, B. Halac, B. B. Kahraman

{"title":"Otitis Externa in Dogs: Distribution and Antimicrobial Susceptibility Patterns of Staphylococcus Spp. Isolates","authors":"Mohammad. H. A. Hassan, A. Kekec, B. Halac, B. B. Kahraman","doi":"10.2478/macvetrev-2023-0012","DOIUrl":"https://doi.org/10.2478/macvetrev-2023-0012","url":null,"abstract":"Abstract The study aimed to investigate the Staphylococcus species from dogs with chronic otitis externa in Istanbul and to determine their antibiotic susceptibility patterns. Ear swab samples were collected from 100 dogs suspected of otitis externa admitted at the clinics of Istanbul University - Cerrahpaşa, Faculty of Veterinary Medicine. The dogs were of different age, gender, and breed. The bacterial isolation was performed by conventional methods. BD Phoenix Automated Microbiology System was used to confirm bacterial identification by conventional methods and to test antimicrobial susceptibility. Staphylococcus spp. were isolated from 36% of the samples collected from the dogs. S. pseudintermedius, S. aureus, S. epidermidis, S. hyicus and S. chromogenes were identified in 41.6%, 22.2%, 11.1%, 5.5%, and 5.5%. In Staphylococcus spp. isolates, enrofloxacin, penicillin, and ampicillin-sulbactam resistance was 8.3%, marbofloxacin resistance was 11.1%, doxycycline resistance was 16.6%, amoxicillin-clavulanic acid, erythromycin, and gentamicin resistance was 19.4%, tetracycline, clindamycin, and sulphonamide resistance was 25%. Methicillin resistance was not observed in any of the isolates. However, multiple drug resistance (MDR) was detected in 11 (30.5%) of 36 isolates. In conclusion, the early detection and antimicrobial sensitivity testing of Staphylococcus spp in dog otitis externa cases that do not respond to empiric therapy could be beneficial for appropriate antibiotic selection and treatment thus preventing MDR.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"46 1","pages":"43 - 50"},"PeriodicalIF":0.4,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41945016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Mastits Incidence In Small-Scale Dairy Cow Farms 小规模奶牛养殖场的临床乳腺炎发病率

IF 0.4

Macedonian Veterinary Review Pub Date : 2023-01-19 DOI: 10.2478/macvetrev-2023-0010

Aleksandar Janevski, M. Nikolovski, Iskra Cvetkovikj, Silvana Andreevska, Angjelche Todorovski, D. Mitrov

{"title":"Clinical Mastits Incidence In Small-Scale Dairy Cow Farms","authors":"Aleksandar Janevski, M. Nikolovski, Iskra Cvetkovikj, Silvana Andreevska, Angjelche Todorovski, D. Mitrov","doi":"10.2478/macvetrev-2023-0010","DOIUrl":"https://doi.org/10.2478/macvetrev-2023-0010","url":null,"abstract":"Abstract The incidence of clinical mastitis (CM) in small and large-scale dairy cow farms can be highly variable and can be affected by age, parity, post-calving status, and atmospheric conditions. The current study aimed to assess the CM-incidence and its association with the post-calving days, number of lactations, season, and number of affected udder quarters in dairy cows in small-scale dairy farms. The study was conducted within one calendar year in 177 small-scale farms with 864 dairy cows. Clinically confirmed CM cows (n=72) were sampled from each udder quarter and processed for bacteriology examination. The CM-positive samples were grouped according to the season (spring, summer, autumn, and winter), the number of days after calving (<90, 90-180, and >180), the number of lactations (1-st, 2-5-th, and >6-th), and the number of affected udder quarters (one, two, three, and four). The CM-positive samples (n=72, 8.3%) were confirmed on bacteriology examination in significantly lower count (n=56, 6.5%). The 2-5-th lactations cows (n=35, 68.6%) were significantly more compared to the first lactation (n=6, 11.8%), and >6-th lactation cows (n=10, 19.6%). CM cows with infection of one udder quarter (n=40, 78.4%) were significantly more than the cows with two (n=6, 11.8%), and four infected quarters (n=5, 9.8%). The CM-incidence in small-scale dairy cow farms in North Macedonia was 8.3% and 6.5% by clinical and bacteriology examination, respectively. The highest CM susceptibility was observed in the cows between the second and fifth lactations. One udder quarter was most frequently affected in CM-positive cases.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"46 1","pages":"35 - 41"},"PeriodicalIF":0.4,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42335548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Art and Science of Consultations in Bovine Medicine: Use of Modified Calgary – Cambridge Guides, Part 2 牛医学咨询的艺术与科学：修改后的卡尔加里-剑桥指南的使用，第2部分

IF 0.4

Macedonian Veterinary Review Pub Date : 2023-01-19 DOI: 10.2478/macvetrev-2023-0011

Amanda N. Carr, R. Kirkwood, K. Petrovski

{"title":"The Art and Science of Consultations in Bovine Medicine: Use of Modified Calgary – Cambridge Guides, Part 2","authors":"Amanda N. Carr, R. Kirkwood, K. Petrovski","doi":"10.2478/macvetrev-2023-0011","DOIUrl":"https://doi.org/10.2478/macvetrev-2023-0011","url":null,"abstract":"Abstract This article, part 2 of a 2-part series, describes the next two steps in the application of the Modified Calgary-Cambridge Guides (MCCG) to consultations in bovine medicine, ‘explanation and planning’, and ‘closing the consultation’, and introduces concepts that are associated with all the components of the guide, ‘building the relationship with the client’ and ‘providing structure to the consultation’. Part 1 introduced the aim and framework of the MCCG which enables the practitioner to gain an insight into the client’s understanding of the problem, including understanding aetiology, epidemiology and pathophysiology. Part 2 introduces the framework that provides the opportunity to understand the client’s expectations regarding the outcome, their motivation and willingness to adhere to recommendations. It also describes how to engage and acknowledge the client as an important part of the decision-making process, how to establish responsibilities of both the client and practitioner, and how to reach out to the client at the conclusion of the consultation to make certain that the client’s expectations were met.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"46 1","pages":"5 - 16"},"PeriodicalIF":0.4,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46199018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Resistance and Susceptibility to Prion Diseases in Pelibuey Sheep from Mexico 墨西哥Pelibuey羊对朊病毒疾病的抗性和敏感性评价

IF 0.4

Macedonian Veterinary Review Pub Date : 2022-12-31 DOI: 10.2478/macvetrev-2022-0030

Fabiola Rodríguez-Andrade, Arnulfo Villanueva-Castillo, César Pastelín-Rojas, Raymundo Avila-Benitez, R. S. Moreno-Mejía, José Alfredo Galicia-Domínguez

{"title":"Evaluation of Resistance and Susceptibility to Prion Diseases in Pelibuey Sheep from Mexico","authors":"Fabiola Rodríguez-Andrade, Arnulfo Villanueva-Castillo, César Pastelín-Rojas, Raymundo Avila-Benitez, R. S. Moreno-Mejía, José Alfredo Galicia-Domínguez","doi":"10.2478/macvetrev-2022-0030","DOIUrl":"https://doi.org/10.2478/macvetrev-2022-0030","url":null,"abstract":"Abstract Transmissible spongiform encephalopathies (TSEs) are a group of neurodegenerative diseases with a chronic and fatal course, which are caused by a misfolded form of the cellular prion protein that is encoded by the host. The purpose of this work was to evaluate the resistance and genetic susceptibility to prion diseases in Pelibuey sheep from Mexico. The sequences of 99 Pelibuey sheep from the central and southwestern regions of Mexico were analyzed to determine the polymorphisms related to resistance and genetic susceptibility to scrapie, the Hardy-Weinberg equilibrium test and the D-Tajima test was used to identify the effect of evolutionary forces on the PRNP gene. Twelve non-synonymous polymorphisms Q101R, M112T, A116P, G127A, A136V, M137T, L141F, H143R, R154H, Q171R, Q171H, N176K were detected, in addition two synonymous substitutions 231R (agg/cgg) and 237L (ctc/ctg) were found. As a result of the sequence analysis, the ARR allele was not under the Hardy-Weinberg equilibrium, indicating that there is an evolutionary force at work, and the D-Tajima suggest the existence of purifying genetic selection. In conclusion, Pelibuey sheep exhibit genotypes for the PRNP gene that make them resistant to developing classic scrapie; at least 98% of the population is a carrier of an allele related to scrapie resistance; while for atypical scrapie there is a very high probability that an outbreak occurred in the herds, since the ARQ allele in combination with the L substitution at codon 141 confers susceptibility to carrier sheep.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"46 1","pages":"17 - 26"},"PeriodicalIF":0.4,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44446174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0