Malaria Research and Treatment最新文献_第10页

Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria. 尼日利亚恶性疟原虫临床诊断、显微镜检查、快速诊断试验和聚合酶链反应的评估

Malaria Research and Treatment Pub Date : 2013-01-01 Epub Date: 2013-11-24 DOI: 10.1155/2013/308069

Olusola Ojurongbe, Olunike Olayeni Adegbosin, Sunday Samuel Taiwo, Oyebode Armstrong Terry Alli, Olugbenga Adekunle Olowe, Taiwo Adetola Ojurongbe, Oloyede Samuel Bolaji, Oluwaseyi Adegboyega Adeyeba

{"title":"Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria.","authors":"Olusola Ojurongbe, Olunike Olayeni Adegbosin, Sunday Samuel Taiwo, Oyebode Armstrong Terry Alli, Olugbenga Adekunle Olowe, Taiwo Adetola Ojurongbe, Oloyede Samuel Bolaji, Oluwaseyi Adegboyega Adeyeba","doi":"10.1155/2013/308069","DOIUrl":"https://doi.org/10.1155/2013/308069","url":null,"abstract":"This study compares the performance of clinical diagnosis and three laboratory diagnostic methods (thick film microscopy (TFM), rapid diagnostic test (RDT), and polymerase chain reaction (PCR)) for the diagnosis of Plasmodium falciparum in Nigeria. Using clinical criteria, 217 children were recruited into the study out of which 106 (48.8%) were positive by TFM, 84 (38.7%) by RDT, and 125 (57.6%) by PCR. Using a composite reference method generated from the three diagnostic methods, 71 (32.7%) patients were found to be truly infected and 90 (41.5%) truly uninfected, while 56 (25.8%) were misidentified as infected or noninfected. When each of the 3 diagnostic methods was compared with the composite reference, PCR had sensitivity of 97.3%, specificity of 62.5%, positive predictive value (PPV) of 56.8%, and negative predictive value (NPV) of 97.8%; microscopy had sensitivity of 77.2%, specificity of 72%, PPV of 66.9%, and NPV of 81.1%, while RDT had sensitivity of 62.3%, specificity of 87.4%, PPV of 67.7%, and NPV of 84.5%. PCR test performed best among the three methods followed by TFM and RDT in that order. The result of this study shows that clinical diagnosis cannot be relied upon for accurate diagnosis of P. falciparum in endemic areas. ","PeriodicalId":18089,"journal":{"name":"Malaria Research and Treatment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/308069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31984164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Selective intermittent preventive treatment of vivax malaria: reduction of malaria incidence in an open cohort study in brazilian Amazon. 间日疟的选择性间歇预防治疗:巴西亚马逊地区一项开放式队列研究中疟疾发病率的降低。

Malaria Research and Treatment Pub Date : 2013-01-01 Epub Date: 2013-03-21 DOI: 10.1155/2013/310246

Tony Hiroshi Katsuragawa, Luiz Herman Soares Gil, Alzemar Alves de Lima, Elci Marlei Freitag, Tatiana Marcondes Dos Santos, Maria Teixeira do Nascimento Filha, Alcides Procópio Justiniano Dos Santos Júnior, Josiane Mendes da Silva, Aline de Freitas Rodrigues, Mauro Shugiro Tada, Cor Jesus Fernandes Fontes, Luiz Hildebrando Pereira da Silva

{"title":"Selective intermittent preventive treatment of vivax malaria: reduction of malaria incidence in an open cohort study in brazilian Amazon.","authors":"Tony Hiroshi Katsuragawa, Luiz Herman Soares Gil, Alzemar Alves de Lima, Elci Marlei Freitag, Tatiana Marcondes Dos Santos, Maria Teixeira do Nascimento Filha, Alcides Procópio Justiniano Dos Santos Júnior, Josiane Mendes da Silva, Aline de Freitas Rodrigues, Mauro Shugiro Tada, Cor Jesus Fernandes Fontes, Luiz Hildebrando Pereira da Silva","doi":"10.1155/2013/310246","DOIUrl":"https://doi.org/10.1155/2013/310246","url":null,"abstract":"In children, the Intermittent Preventive Treatment (IPTc), currently called Seasonal Malaria Chemoprevention (SMC), was considered effective on malaria control due to the reduction of its incidence in Papua New Guinea and in some areas with seasonal malaria in Africa. However, the IPT has not been indicated because of its association with drug resistance and for hindering natural immunity development. Thus, we evaluated the alternative IPT impact on malaria incidence in three riverside communities on Madeira River, in the municipality of Porto Velho, RO. We denominate this scheme Selective Intermittent Preventive Treatment (SIPT). The SIPT consists in a weekly dose of two 150 mg chloroquine tablets for 12 weeks, for adults, and an equivalent dose for children, after complete supervised treatment for P. vivax infection. This scheme is recommend by Brazilian Health Ministry to avoid frequent relapses. The clinic parasitological and epidemiological surveillance showed a significant reduction on vivax malaria incidence. The results showed a reduction on relapses and recurrence of malaria after SIPT implementation. The SIPT can be effective on vivax malaria control in localities with high transmission risk in the Brazilian Amazon.","PeriodicalId":18089,"journal":{"name":"Malaria Research and Treatment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/310246","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31350712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Malaria in tunisian military personnel after returning from external operation. 突尼斯军事人员从外部行动返回后的疟疾。

Malaria Research and Treatment Pub Date : 2013-01-01 Epub Date: 2013-05-23 DOI: 10.1155/2013/359192

Faïda Ajili, Riadh Battikh, Janet Laabidi, Rim Abid, Najeh Bousetta, Bouthaina Jemli, Nadia Ben Abdelhafidh, Louzir Bassem, Saadia Gargouri, Salah Othmani

{"title":"Malaria in tunisian military personnel after returning from external operation.","authors":"Faïda Ajili, Riadh Battikh, Janet Laabidi, Rim Abid, Najeh Bousetta, Bouthaina Jemli, Nadia Ben Abdelhafidh, Louzir Bassem, Saadia Gargouri, Salah Othmani","doi":"10.1155/2013/359192","DOIUrl":"https://doi.org/10.1155/2013/359192","url":null,"abstract":"Introduction. Malaria had been eliminated in Tunisia since 1979, but there are currently 40 to 50 imported cases annually. Soldiers are no exception as the incidence of imported malaria is increasing in Tunisian military personnel after returning from malaria-endemic area, often in Sub-Saharan Africa. Methods. We retrospectively analyzed the clinical and biological presentations, treatment, and outcomes of 37 Tunisian military personnel hospitalized at the Department of Internal Medicine, the Military Hospital of Tunis, between January 1993 and January 2011, for imported malaria. The clinical and laboratory features were obtained from the medical records and a questionnaire was filled by the patients about the compliance of malaria prophylaxis. Results. Thirty-seven male patients, with a mean age of 41 years, were treated for malaria infection. Twenty-two were due to Plasmodium falciparum. The outcome was favourable for all patients, despite two severe access. The long-term use of chemoprophylaxis has been adopted by only 21 (51%) of expatriate military for daily stresses. Moreover, poor adherence was found in 32 patients. Conclusion. The risk of acquiring malaria infection in Tunisian military personnel can largely be prevented by the regular use of chemoprophylactic drugs combined with protective measures against mosquito bites.","PeriodicalId":18089,"journal":{"name":"Malaria Research and Treatment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/359192","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31506421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Expanding Access to Malaria Diagnosis through Retail Shops in Western Kenya: What Do Shop Workers Think? 通过肯尼亚西部的零售商店扩大疟疾诊断:商店工作人员怎么想?

Malaria Research and Treatment Pub Date : 2013-01-01 Epub Date: 2013-05-21 DOI: 10.1155/2013/398143

Andria Rusk, Catherine Goodman, Violet Naanyu, Beatrice Koech, Andrew Obala, Wendy Prudhomme O'Meara

{"title":"Expanding Access to Malaria Diagnosis through Retail Shops in Western Kenya: What Do Shop Workers Think?","authors":"Andria Rusk, Catherine Goodman, Violet Naanyu, Beatrice Koech, Andrew Obala, Wendy Prudhomme O'Meara","doi":"10.1155/2013/398143","DOIUrl":"https://doi.org/10.1155/2013/398143","url":null,"abstract":"Background. The common symptoms of malaria reduce the specificity of clinical diagnosis. Presumptive treatment is conventional but can lead to overdiagnosis of malaria, delay of appropriate treatment, overprescription of antimalarials, and drug resistance. Routine use of diagnostic tests can address many of these concerns. Though treatment is often procured from retailers, there is low availability of rapid diagnostic tests for malaria (MRDTs), a simple, inexpensive, and accurate diagnostic solution. We know little about the challenges to expanding access to diagnostics through these outlets. Methods. To understand the perceptions of the benefits and challenges to selling rapid diagnostic tests for malaria, we conducted focus group discussions with antimalarial retailers who serve the residents of the Webuye Health and Demographic Surveillance Site in western Kenya. Results. Medicine retailers perceived MRDTs to be beneficial to their customers and businesses but also included cost, fear of the tests, risks of self-treatment, and regulatory concerns among the challenges to using and selling MRDTs. Conclusion. MRDTs represent a viable approach to increase access to malaria diagnostic testing. Medicine retailers are eager for MRDTs to be made available to them. However, certain challenges remain to implementation in retail outlets and should be addressed in advance.","PeriodicalId":18089,"journal":{"name":"Malaria Research and Treatment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/398143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31506422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Malariometric Indices among Nigerian Children in a Rural Setting. 尼日利亚农村儿童的疟疾测量指数。

Malaria Research and Treatment Pub Date : 2013-01-01 Epub Date: 2013-02-28 DOI: 10.1155/2013/716805

Ekong E Udoh, Angela E Oyo-Ita, Friday A Odey, Komomo I Eyong, Chioma M Oringanje, Olabisi A Oduwole, Joseph U Okebe, Ekpereonne B Esu, Martin M Meremikwu, Asindi A Asindi

{"title":"Malariometric Indices among Nigerian Children in a Rural Setting.","authors":"Ekong E Udoh, Angela E Oyo-Ita, Friday A Odey, Komomo I Eyong, Chioma M Oringanje, Olabisi A Oduwole, Joseph U Okebe, Ekpereonne B Esu, Martin M Meremikwu, Asindi A Asindi","doi":"10.1155/2013/716805","DOIUrl":"https://doi.org/10.1155/2013/716805","url":null,"abstract":"Malaria contributes to high childhood morbidity and mortality in Nigeria. To determine its endemicity in a rural farming community in the south-south of Nigeria, the following malariometric indices, namely, malaria parasitaemia, spleen rates, and anaemia were evaluated in children aged 2-10 years. This was a descriptive cross-sectional survey among school-age children residing in a rubber plantation settlement. The children were selected from six primary schools using a multistaged stratified cluster sampling technique. They were all examined for pallor, enlarged spleen, or liver among other clinical parameters and had blood films for malaria parasites. Of the 461 children recruited, 329 (71.4%) had malaria parasites. The prevalence of malaria parasitaemia was slightly higher in the under fives than that of those ≥5 years, 76.2% and 70.3%, respectively. Splenic enlargement was present in 133 children (28.9%). The overall prevalence of anaemia was 35.7%. Anaemia was more common in the under-fives (48.8%) than in those ≥5 years (32.8%). The odds of anaemia in the under fives were significantly higher than the odds of those ≥5 years (OR = 1.95 [1.19-3.18]). Malaria is highly endemic in this farming community and calls for intensification of control interventions in the area with special attention to school-age children.","PeriodicalId":18089,"journal":{"name":"Malaria Research and Treatment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/716805","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40228988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Awareness and Utilization of Affordable Medicine Facility-Malaria among Caregivers of Under-Five Children in Ibadan North-West Local Government Area, Oyo State. 奥约州伊巴丹西北地方政府区五岁以下儿童护理人员对负担得起的医疗设施的认识和利用-疟疾

Malaria Research and Treatment Pub Date : 2013-01-01 Epub Date: 2013-12-30 DOI: 10.1155/2013/176096

Ikeoluwapo O Ajayi, Tolulope Soyannwo, Onoja M Akpa

{"title":"Awareness and Utilization of Affordable Medicine Facility-Malaria among Caregivers of Under-Five Children in Ibadan North-West Local Government Area, Oyo State.","authors":"Ikeoluwapo O Ajayi, Tolulope Soyannwo, Onoja M Akpa","doi":"10.1155/2013/176096","DOIUrl":"10.1155/2013/176096","url":null,"abstract":"Introduction. Distribution of Affordable Medicine Facility-malaria Artemisinin Combination Therapies (AMFm-ACTs) started in Nigeria in 2011, but its use at community level has not been documented. Methods. Four hundred seventy-eight caregivers whose under-five children had fever within two weeks prior to the survey were selected using cluster sampling technique. Information on sociodemographic characteristics, treatment seeking for malaria, and awareness and use of AMFm-ACTs was collected using an interviewer administered questionnaire. Result. More than half of the respondents (51.2%) bought AMFm-ACTs without prescription. Awareness of AMFm was low as only 9.1% has heard about the programme. Overall, 29.2% used AMFm-ACTs as their first line choice of antimalarial drug. On bivariate analysis age, group (25-34 years), public servants, respondents with tertiary education, respondents with high socioeconomic status, respondents with poor knowledge of symptoms of malaria, awareness of AMFm-ACTs, availability of AMFm-ACTs, and sources of drug were significantly associated with utilization of AMFm-ACTs (P < 0.05). Logistic regression demonstrated that only people who were aware of AMFM-ACTs predicted its use (AOR: 0.073; CI: 0.032-0.166; P < 0.001). Conclusion. Interventions which targeted at raising awareness of AMFm-ACTs among people at risk of malaria are advocated for implementation. ","PeriodicalId":18089,"journal":{"name":"Malaria Research and Treatment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/176096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32085276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Management of uncomplicated malaria in underfives in private and public health facilities in South-eastern Nigeria: a clinical audit of current practices. 尼日利亚东南部私营和公共卫生机构五岁以下儿童无并发症疟疾的管理:对当前做法的临床审计。

Malaria Research and Treatment Pub Date : 2013-01-01 Epub Date: 2013-01-21 DOI: 10.1155/2013/575080

Ekong Udoh, Angela Oyo-Ita, Friday Odey, Emmanuel Effa, Ekpereonne Esu, Olabisi Oduwole, Moriam Chibuzor, Martin Meremikwu

{"title":"Management of uncomplicated malaria in underfives in private and public health facilities in South-eastern Nigeria: a clinical audit of current practices.","authors":"Ekong Udoh, Angela Oyo-Ita, Friday Odey, Emmanuel Effa, Ekpereonne Esu, Olabisi Oduwole, Moriam Chibuzor, Martin Meremikwu","doi":"10.1155/2013/575080","DOIUrl":"https://doi.org/10.1155/2013/575080","url":null,"abstract":"Malaria remains a leading cause of underfive morbidity and mortality in sub-Saharan Africa. Effective case management is a strategy recommended by the World Health Organization for its control. A clinical audit of case management of uncomplicated malaria in underfives in health facilities in Cross River State, Nigeria, was conducted from January to March 2012. Data was extracted from patients' case records by trained medical personnel using pretested data extraction forms. Of the 463 case records reviewed, age, gender, and weight were reported in 98.1%, 97.3%, and 49.7% of the children, respectively. A history of fever was obtained in 89.6% and a record of temperature in 74.1% of the children. General examination was performed in 203 (43.8%) children. Malaria parasite test was requested in 132 (28.5%) while Packed cell volume or haemoglobin was requested in 107 (23.1%) children. Appropriate dose of Artemisinin Combination Therapy (ACT) was instituted in 300 (64.8%), wrong dose in 109 (23.5%), and inappropriate treatment in 41 (8.9%). The utilization of ACTs for treating uncomplicated malaria in the State has improved but clinical assessment of patients and laboratory confirmation of diagnosis are suboptimum.","PeriodicalId":18089,"journal":{"name":"Malaria Research and Treatment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/575080","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31323038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Failure of Supervised Chloroquine and Primaquine Regimen for the Treatment of Plasmodium vivax in the Peruvian Amazon. 秘鲁亚马逊地区氯喹和伯氨喹治疗间日疟原虫的失败。

Malaria Research and Treatment Pub Date : 2012-01-01 Epub Date: 2012-05-31 DOI: 10.1155/2012/936067

Paul C F Graf, Salomon Durand, Carlos Alvarez Antonio, Carmen Montalvan, Mariella Galves Montoya, Michael D Green, Meddly L Santolalla, Carola Salas, Carmen Lucas, David J Bacon, David J Fryauff

{"title":"Failure of Supervised Chloroquine and Primaquine Regimen for the Treatment of Plasmodium vivax in the Peruvian Amazon.","authors":"Paul C F Graf, Salomon Durand, Carlos Alvarez Antonio, Carmen Montalvan, Mariella Galves Montoya, Michael D Green, Meddly L Santolalla, Carola Salas, Carmen Lucas, David J Bacon, David J Fryauff","doi":"10.1155/2012/936067","DOIUrl":"https://doi.org/10.1155/2012/936067","url":null,"abstract":"The widespread use of primaquine (PQ) and chloroquine (CQ), together, may be responsible for the relatively few, isolated cases of chloroquine-resistant P. vivax (CQRPV) that have been reported from South America. We report here a case of P. vivax from the Amazon Basin of Peru that recurred against normally therapeutic blood levels of CQ. Four out of 540 patients treated with combination CQ and PQ had a symptomatic recurrence of P. vivax parasitemia within 35 days of treatment initiation, possibly indicating CQ failure. Whole blood total CQ level for one of these four subjects was 95 ng/ml on the day of recurrence. Based on published criteria that delineate CQRPV as a P. vivax parasitemia, either recrudescence or relapse, that appears against CQ blood levels >100 ng/mL, we document the occurrence of a P. vivax strain in Peru that had unusually high tolerance to the synergistic combination therapy of CQ + PQ that normally works quite well.","PeriodicalId":18089,"journal":{"name":"Malaria Research and Treatment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/936067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30693043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Antimalarial Effects of Iranian Flora Artemisia sieberi on Plasmodium berghei In Vivo in Mice and Phytochemistry Analysis of Its Herbal Extracts. 伊朗植物细叶蒿对小鼠体内伯格氏疟原虫的抗疟作用及其提取物的植物化学分析。

Malaria Research and Treatment Pub Date : 2012-01-01 Epub Date: 2012-01-23 DOI: 10.1155/2012/727032

Hossein Nahrevanian, Bayram Sheykhkanlooye Milan, Masoud Kazemi, Reza Hajhosseini, Soudeh Soleymani Mashhadi, Shahab Nahrevanian

{"title":"Antimalarial Effects of Iranian Flora Artemisia sieberi on Plasmodium berghei In Vivo in Mice and Phytochemistry Analysis of Its Herbal Extracts.","authors":"Hossein Nahrevanian, Bayram Sheykhkanlooye Milan, Masoud Kazemi, Reza Hajhosseini, Soudeh Soleymani Mashhadi, Shahab Nahrevanian","doi":"10.1155/2012/727032","DOIUrl":"https://doi.org/10.1155/2012/727032","url":null,"abstract":"The aim of this study is pharmacochemistry of Iranian flora Artemisia sieberi and its antimalarial effects on Plasmodium berghei in vivo. This is the first application of A. sieberi for treatment of murine malaria. A. sieberi were collected at flowering stage from the Khorassan and Semnan provinces of Iran; the aerial parts were air-dried at room temperature and then powdered. The powder was macerated in methanol, filtered with Bokhner hopper and solvent was separated in rotary evaporator. Total herbal extract was subsequently processed for ether and chloroform extracts preparation. The toxicity of herbal extract was assessed on naive NMRI mice with high, average and low doses; then pathophysiological signs were assessed. Finally, the antimalarial efficacy was investigated on two groups of Plasmodium berghei infected mice. Percentage of parasitaemia and pathophysiology were also evaluated. The results of this assessment showed no toxicity even by high concentration of herbal extract. A significant reduction in percentage of parasitaemia was observed; no alterations of hepatosplenomegaly and body weight were indicated in study group. A. sieberi extracts showed antimalarial effects against murine malaria with some efficacies on reducing pathophysiology. However, there is requirement to find the major component of this herbal extract by further studies.","PeriodicalId":18089,"journal":{"name":"Malaria Research and Treatment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/727032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30443973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Comparative Study on the Effects of Chloroquine and Artesunate on Histopathological Damages Caused by Plasmodium berghei in Four Vital Organs of Infected Albino Mice. 氯喹与青蒿琥酯对白化病小鼠4个重要脏器伯氏疟原虫组织病理损伤的比较研究。

Malaria Research and Treatment Pub Date : 2012-01-01 Epub Date: 2012-06-24 DOI: 10.1155/2012/960758

O T Soniran, O A Idowu, O L Ajayi, I C Olubi

{"title":"Comparative Study on the Effects of Chloroquine and Artesunate on Histopathological Damages Caused by Plasmodium berghei in Four Vital Organs of Infected Albino Mice.","authors":"O T Soniran, O A Idowu, O L Ajayi, I C Olubi","doi":"10.1155/2012/960758","DOIUrl":"https://doi.org/10.1155/2012/960758","url":null,"abstract":"The aim of the present study was to investigate the positive influence of chloroquine and artesunate on the pathological damages caused by Plasmodium berghei on vital organs of mice in an established infection. Healthy adult albino mice with average weight of 25 g were used for the study. Treated group was administered orally with 100 mg/kg of chloroquine and artesunate, respectively. Control animals were given water for the same period. Histological examination of the liver, spleen, lungs, and kidney revealed absence of accumulation of iron (haemosiderosis) in the liver, thickened alveolar wall, and interstitial mononuclear cells infiltration in the lungs of the artesunate group, while absence of emphysema in the lungs and megakaryoblast hyperplasia in the spleen was observed in the chloroquine group. Lymphoid hypoplasia in the chloroquine group and megakayoblast hyperplasia in the artesunate group were observed but not in the control group. Thus, the use of these drugs especially under the practice of self-medication should be prohibited in lands where they are still in use as antimalaria medicine.","PeriodicalId":18089,"journal":{"name":"Malaria Research and Treatment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/960758","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30760906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21