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[Determination of the blood thiol disulfide balance using the coulometric titration method]. 库仑滴定法测定血中硫醇二硫平衡
Laboratornoe delo Pub Date : 1991-01-01
S V Zits
{"title":"[Determination of the blood thiol disulfide balance using the coulometric titration method].","authors":"S V Zits","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 8","pages":"33-5"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12888238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[An automated scintillation counter for poorly contrasting microparticles]. [用于对比差的微粒的自动闪烁计数器]。
Laboratornoe delo Pub Date : 1991-01-01
E V Gusev, E A Mesropian, Iu M Rozanov, A G Ovanesian
{"title":"[An automated scintillation counter for poorly contrasting microparticles].","authors":"E V Gusev,&nbsp;E A Mesropian,&nbsp;Iu M Rozanov,&nbsp;A G Ovanesian","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A cell scintillator intended for analysis of the disperse composition and concentration of poor contrast biologic objects suspended in transparent liquid media has been designed at the Analitpribor Research and Production Amalgamation in Tbilisi for the Cytology Institute of the USSR Academy of Sciences in Leningrad. The apparatus is based on light blocking and hydro-focussing physical principles. Automated system of the apparatus calibration permits measurements of the particles 3 to 150 microns in size at the level of 10%. The mean error of cell concentration estimation within the range of 10000-150000 cell/ml is 3%.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 5","pages":"12-5"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12879941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Lactoferrin and fibrinogen degradation products in saliva]. 唾液中的乳铁蛋白和纤维蛋白原降解产物。
Laboratornoe delo Pub Date : 1991-01-01
A E Sukharev, A A Nikolaev, N N Nikolaeva, N A Sukhareva
{"title":"[Lactoferrin and fibrinogen degradation products in saliva].","authors":"A E Sukharev,&nbsp;A A Nikolaev,&nbsp;N N Nikolaeva,&nbsp;N A Sukhareva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The authors suggest a simple and convenient procedure for measuring acute-phase proteins, lactoferrin, fibrinogen and its degradation products, in mixed human saliva by the agar immunodiffusion test with appropriate monospecific antisera. Examinations of 1205 salivary samples have revealed that salivary fibrinogen degradation products and lactoferrin detection rates do not depend on the subject's sex but are growing with age and intensity of the aggressive admixture effects in inhaled air; they are also related to the intensity of the inflammatory and proliferative processes in the oral cavity and bronchopulmonary system, the values varying from 4-16 percent in health to 68.8-71.8 percent in disease. In monitoring salivary acute-phase proteins detection rates make up 100 percent in the patients and 0-4 percent in normal subjects. The authors come to a conclusion that measurements of salivary acute-phase proteins may be used in screening of the population to detect subjects at risk for further diagnostic examinations.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 5","pages":"20-4"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12879944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A method of determining the adhesive properties of sputum]. 一种测定痰液黏附性的方法。
Laboratornoe delo Pub Date : 1991-01-01
A A Punin
{"title":"[A method of determining the adhesive properties of sputum].","authors":"A A Punin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Adhesive characteristics of the sputum are assessed from adhesion to glass. The force and time of preliminary pressure on the tested material are not taken into consideration, nor is the rate of augmentation of the force directed to disruption of the adhesion contact. The strength of the adhesion contact was found directly depending on the force and time of preliminary pressure and inversely related to disrupting force augmentation rate. A conclusion is made on the necessity of stabilizing work schedules (in reference to the above parameters) for the standardization of the method.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 5","pages":"29-31"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12879947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A method of determining glucose-6-phosphate dehydrogenase in bacteria of the genus Yersinia]. 测定耶尔森菌属细菌中葡萄糖-6-磷酸脱氢酶的方法。
Laboratornoe delo Pub Date : 1991-01-01
G S Gureeva, V G Maĭskiĭ, B P Tsybin, E A Lunina
{"title":"[A method of determining glucose-6-phosphate dehydrogenase in bacteria of the genus Yersinia].","authors":"G S Gureeva,&nbsp;V G Maĭskiĭ,&nbsp;B P Tsybin,&nbsp;E A Lunina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for qualitative assessment of glucose-6-phosphate dehydrogenase in Yersinia is suggested. The reaction mixture was prepared by mixing the ingredients in the ratios as follows: 10 microliters of 0.01 M glucose-6-phosphate, 10 microliters of 0.0075 M NADP, 30 microliters of 0.75 M tris HCl pH 7.8, 10 microliters of 0.2 M MgCl2, 20 microliters of distilled water. The reaction was triggered by adding 20 microliters of cell-free bacterial extract. After 110-130 min incubation 10-20 microliters aliquots w re collected on filter paper (Whatman No 1) strips. The stains were dried at ambient temperature and examined in UV light at 365 nm with the use of a routine UV lamp. This method shows intensive fluorescence of the stain in M. tuberculosis and very poor fluorescence in Y. pestis. This technique is convenient when the scope of identification tests and differentiation between these two agents is high.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 5","pages":"56-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12880435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The aldehyde dehydrogenase activity in the erythrocytes of alcoholic patients]. [酒精中毒患者红细胞中醛脱氢酶活性]。
Laboratornoe delo Pub Date : 1991-01-01
I M Matveeva, M A Petrova, M S Usatenko, N E Sokolovskaia, T G Rybakova, Iu S Borodkin
{"title":"[The aldehyde dehydrogenase activity in the erythrocytes of alcoholic patients].","authors":"I M Matveeva,&nbsp;M A Petrova,&nbsp;M S Usatenko,&nbsp;N E Sokolovskaia,&nbsp;T G Rybakova,&nbsp;Iu S Borodkin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Red cell aldehyde dehydrogenase (ALDH, EC 1.2.1.3) activity was measured by spectrophotometry in normal subjects and alcoholics after various periods of alcohol abstinence. ALDH was measured in all red cell hemolysate fractions; red cells were purified from hemoglobin by Sephadex CM-50 chromatography. The enzyme activity was found reduced in alcoholics' red cells as against that in controls. ALDH activities were somewhat increasing and approaching the normal values in the patients not using ethanol for 4 weeks or longer. These results recommend ALDH measurements in human red cells as a test for the detection of subjects abusing alcohol.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 6","pages":"20-4"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12882235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A method of determining glucose oxidase-immobilized glucose]. [测定葡萄糖氧化酶固定葡萄糖的方法]。
Laboratornoe delo Pub Date : 1991-01-01
I P Ivanov, S I Danev, D G Dimitrov
{"title":"[A method of determining glucose oxidase-immobilized glucose].","authors":"I P Ivanov,&nbsp;S I Danev,&nbsp;D G Dimitrov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for manual measurement of glucose in biologic fluids has been developed, making use of glucose oxidase immobilized on a carbamide derivative of microcrystal cellulose; two variants are suggested: a rapid and a routine one. The method is characterized by a high analytical reliability, its results are in high correlation with the results of measurements by Beckman glucose analyzer (r = 0.92, p less than 0.001). The method is economic (glucose oxidase reagent may be used for more than 300 times), easily available, and is 3 to 6 times more rapid than the method with soluble glucose oxidase. It is particularly convenient for urgent laboratory diagnosis.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 10","pages":"28-30"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12885733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The activity of meningococcal agglutinating sera in coagglutination and precipitation reactions]. [凝固沉淀反应中脑膜炎球菌凝集血清的活性]。
Laboratornoe delo Pub Date : 1991-01-01
D B Kaziakhmedova, B S Goncharov, A G Trushnikova
{"title":"[The activity of meningococcal agglutinating sera in coagglutination and precipitation reactions].","authors":"D B Kaziakhmedova,&nbsp;B S Goncharov,&nbsp;A G Trushnikova","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 2","pages":"63-5"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12873666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Determination of the monoamine oxidase and the diamine oxidase activity of a single blood sample]. [单血单胺氧化酶和二胺氧化酶活性的测定]。
Laboratornoe delo Pub Date : 1991-01-01
G A Sivoraksha, Iu N Sidel'nikov
{"title":"[Determination of the monoamine oxidase and the diamine oxidase activity of a single blood sample].","authors":"G A Sivoraksha,&nbsp;Iu N Sidel'nikov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The known methods for simultaneous measurements of blood histamine and serotonin have prompted the authors to search for approaches to simultaneous measurements of monoamine oxidase and diamine oxidase, the enzymes contributing to serotonin and histamine inactivation. The authors suggest a method for simultaneous measurements of the blood serum monoamine oxidase and diamine oxidase activities that permits cut down the time of the blood and substrate incubation to 10 min, essentially shortens the procedure and simplifies it. The method is recommended for research and practice.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 2","pages":"51-4"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12874069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A method of detecting phages in lysogenic strains of Vibrio cholerae]. 一种检测霍乱弧菌溶原菌株中噬菌体的方法。
Laboratornoe delo Pub Date : 1991-01-01
T A Kudriakova, L D Makedonova, L R Cherkasova, O V Dudkina
{"title":"[A method of detecting phages in lysogenic strains of Vibrio cholerae].","authors":"T A Kudriakova,&nbsp;L D Makedonova,&nbsp;L R Cherkasova,&nbsp;O V Dudkina","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 2","pages":"56-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12874071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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