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[An evaluation of methods of determining the hemolytic activity of Klebsiella in solid nutrient media]. [克雷伯氏菌在固体营养培养基中溶血活性测定方法的评价]。
Laboratornoe delo Pub Date : 1991-01-01
S I Pokhil, L A Kosobutskiĭ
{"title":"[An evaluation of methods of determining the hemolytic activity of Klebsiella in solid nutrient media].","authors":"S I Pokhil,&nbsp;L A Kosobutskiĭ","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hemolytic activities of Klebsiella-237 strains of various origins were measured in solid nutrient media by three methods making use of six different agar bases for the preparation of blood nutrient media. The authors analyze the agar bases, discuss the advantages and shortcomings of all the methods used with consideration for the purpose of the investigation. The findings evidence a higher hemolytic activity in Klebsiella isolated from patients as against the hemolytic activities of reference strains.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 6","pages":"49-52"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12882248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A method of determining glucose oxidase-immobilized glucose]. [测定葡萄糖氧化酶固定葡萄糖的方法]。
Laboratornoe delo Pub Date : 1991-01-01
I P Ivanov, S I Danev, D G Dimitrov
{"title":"[A method of determining glucose oxidase-immobilized glucose].","authors":"I P Ivanov,&nbsp;S I Danev,&nbsp;D G Dimitrov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for manual measurement of glucose in biologic fluids has been developed, making use of glucose oxidase immobilized on a carbamide derivative of microcrystal cellulose; two variants are suggested: a rapid and a routine one. The method is characterized by a high analytical reliability, its results are in high correlation with the results of measurements by Beckman glucose analyzer (r = 0.92, p less than 0.001). The method is economic (glucose oxidase reagent may be used for more than 300 times), easily available, and is 3 to 6 times more rapid than the method with soluble glucose oxidase. It is particularly convenient for urgent laboratory diagnosis.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 10","pages":"28-30"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12885733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The chemiluminescent determination of cholesterol]. [化学发光测定胆固醇]。
Laboratornoe delo Pub Date : 1991-01-01
N Iu Filippova, Iu V Rodionov, N N Ugarova
{"title":"[The chemiluminescent determination of cholesterol].","authors":"N Iu Filippova,&nbsp;Iu V Rodionov,&nbsp;N N Ugarova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A chemiluminescent method has been offered to assay the blood serum total cholesterol. The method is based on coupled enzymatic reactions in two steps: (1) esterified cholesterol hydrolysis by cholesterol esterase in the presence of sodium cholate and (2) cholesterol oxidation by cholesterol oxidase and chemiluminescent assay of the released hydrogen peroxide in peroxidase reaction with luminol and p-iodophenol. Separation of these steps results in a better accuracy of the test as against the one-step procedure. High sensitivity of the method (the detection limit being 1 mM of cholesterol in a luminometer cuvette) permits high dilutions of the serum, this essentially eliminating the admixture effects on the enzymatic reactions. The findings of the suggested method are in good correlation with those of the spectrophotometric assay of cholesterol using the KONE kit.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 9","pages":"20-3"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12886113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Evaluation of the intensity of the duodenogastric reflux based on the alkaline phosphatase and trypsin activities in the stomach contents]. [基于胃内容物碱性磷酸酶和胰蛋白酶活性评价十二指肠胃反流强度]。
Laboratornoe delo Pub Date : 1991-01-01
R A Zarembskiĭ, I Iu Kariagina, A F Kerzikov
{"title":"[Evaluation of the intensity of the duodenogastric reflux based on the alkaline phosphatase and trypsin activities in the stomach contents].","authors":"R A Zarembskiĭ,&nbsp;I Iu Kariagina,&nbsp;A F Kerzikov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Alkaline phosphatase and trypsin activities were measured in the gastric contents of patients with chronic gastritis with visually fixed duodenogastric reflux and without reflux. The authors discuss the advantages of a comparative approach to the enzymatic assessment of duodenogastric reflux presence, intensity, and length.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 9","pages":"46-8"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12886123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of heterophilic antibodies on the result of the passive hemagglutination reaction in intestinal infections]. [嗜异性抗体对肠道感染患者被动血凝反应结果的影响]。
Laboratornoe delo Pub Date : 1991-01-01
Iu L Gorchakov
{"title":"[The effect of heterophilic antibodies on the result of the passive hemagglutination reaction in intestinal infections].","authors":"Iu L Gorchakov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>When commercial erythrocytic diagnostic agents, prepared from sheep red cells, are used in the passive hemagglutination test with blood sera from patients with intestinal infections, the possibility of nonspecific reactions with heterophilic antibodies of human body should be borne in mind. To completely eliminate these antibody effects on the results of the test, titration of the tested serum should be started from 1:64, 1:80, or 1:100, depending on what titration scale is used.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 10","pages":"53-5"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12886225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Equipment for microphotography using the Zenit camera]. [使用Zenit相机的缩微摄影设备]。
Laboratornoe delo Pub Date : 1991-01-01
V Ia Dvornik, S A Ovchinnikov
{"title":"[Equipment for microphotography using the Zenit camera].","authors":"V Ia Dvornik,&nbsp;S A Ovchinnikov","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 10","pages":"75"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12886236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Determination of the antioxidant properties of the blood and their diagnostic significance in the elderly]. [老年人血液抗氧化特性的测定及其诊断意义]。
Laboratornoe delo Pub Date : 1991-01-01
S Csóvári, T Andyal, J Strenger
{"title":"[Determination of the antioxidant properties of the blood and their diagnostic significance in the elderly].","authors":"S Csóvári,&nbsp;T Andyal,&nbsp;J Strenger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Blood antioxidant system parameters were examined in elderly subjects. The authors have developed methods for measurements of catalase, superoxide dismutase, and lipid peroxidation products. They introduce a new factor 'F' that is supposed to characterize the blood antioxidant system; this factor is based on the values of catalase and superoxide dismutase activities and the intensity of lipid peroxidation. The authors come to a conclusion that the blood antioxidant and oxidant systems may be more accurately described with the use of this new factor F. In case of an abdominal tumor whole blood catalase level is elevated and superoxide dismutase activity significantly reduced. Factor F values were found extremely low before death, therefore this factor may be considered an important criterion of a critical state. The blood antioxidant parameters of patients with diabetes mellitus and essential hypertension did not much differ from those of age-matched healthy subjects.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 10","pages":"9-13"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12886712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Evaluation of the alternative approach to complement activation using C3-dependent adhesion]. [评价补体活化的替代方法，使用c3依赖性粘附]。
Laboratornoe delo Pub Date : 1991-01-01
A N Maianskiĭ, I V Chebotar', T M Konyshkina, E I Ado, O I Pikuza
{"title":"[Evaluation of the alternative approach to complement activation using C3-dependent adhesion].","authors":"A N Maianskiĭ,&nbsp;I V Chebotar',&nbsp;T M Konyshkina,&nbsp;E I Ado,&nbsp;O I Pikuza","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The authors suggest a method of functional probing of the complement alternative cascade, the jist of it being analysis of the neutrophil adhesion to sephadex granules treated with serum or another complement-containing substrate. The advantages of the method are analyzed, and the range of its application suggested.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 11","pages":"41-4"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12887122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Determination of triglycerides in a high density lipoprotein fraction]. 高密度脂蛋白中甘油三酯的测定
Laboratornoe delo Pub Date : 1991-01-01
L V Kurashvili, L N Bobyleva
{"title":"[Determination of triglycerides in a high density lipoprotein fraction].","authors":"L V Kurashvili,&nbsp;L N Bobyleva","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 7","pages":"75-6"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12887690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A study of acid nuclease activity]. [酸性核酸酶活性的研究]。
Laboratornoe delo Pub Date : 1991-01-01
E A Endakova, T P Novgorodtseva
{"title":"[A study of acid nuclease activity].","authors":"E A Endakova,&nbsp;T P Novgorodtseva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for serial measurements of blood serum acid DNAse (3.1.4.5) and acid RNAse (3.1.4.23). The authors recommend measurements of acid nucleases at clinical biochemistry laboratories and use of these parameters for the prediction of outcomes of balneotherapy and other treatment modalities.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 8","pages":"16-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12888232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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