{"title":"Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats","authors":"Akhmad Afifudin Al-Anshori, Anandia Nafisah Putri, Alif Noviana Ismi, Mohamad Kharis Suhud, Hani Plumeriastuti, Lilik Maslachah","doi":"10.20473/jmv.vol6.iss2.2023.145-154","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss2.2023.145-154","url":null,"abstract":"This study aimed to evaluate the in-vivo burn wound healing process in albino rats treated using transdermal delivery nano ethosomal gel from ashitaba leaves. Ethosomal vesicles were formulated using soy lecithin, cholesterol, ethanol, water, and ashitaba leaf nanoparticles using the cold method. A total of 25 male rats were randomly divided into 5 groups, i.e., (C-) treated without nanoparticle extract, (C+) treated using 1% Silver Sulfadiazine®, treatment group treated nano ethosomal gel from ashitaba leaves with the respective doses were (T1) 1%, (T2) 2,5%, and (T3) 5%. Therapy was initiated on day 1 or after being induced by a burn wound for 14 days, twice a day. On the 15th day, a termination was carried out to take skin tissue from burn scars, then histopathology preparations were made with routine staining. Microscopic observations with an optical microscope on collagen, polymorphonuclear cell infiltration, angiogenesis, and re-epithelialization. The T3 group that was treated using 5% nano ethosomal gel showed the best burn wound healing, this may be caused by compounds in ashitaba leaf nanoparticles which have antioxidant, anti-inflammatory, and antibacterial effects, thus the use of transdermal delivery therapy of 5% ashitaba leaf nano ethosomal gel was effective for wound treatment burn on rat skin.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"1 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135462882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) in Tarakan","authors":"Burhanuddin Ihsan, Rini Septriananda Sitinjak","doi":"10.20473/jmv.vol6.iss2.2023.185-190","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss2.2023.185-190","url":null,"abstract":"Freshwater fisheries product tilapia (Oreochromis niloticus) has the potential to be developed and has a very high economic value, particularly in the city of Tarakan. Fish ectoparasites are a problem that frequently occurs and is discovered in aquaculture. This study aimed to determine the type of ectoparasites that infect tilapia in aquaculture ponds in Kampung Satu and Sebengkok. The technique involved collecting tilapia samples from various ponds and then transporting them to the laboratory. A total of 30 samples were taken from the container that was set up on a tray, and they were all put to death by being stabbed in the brain nerves. It was done to identify ectoparasites on the gills, eyes, scales, and fins of the fish. Two forms of ectoparasites, Dactylogyrus sp. and Trichodina sp., which were only present in the gills, were identified in the tilapia. In the moderate infection group, the prevalence rate in Kampung Satu was 86%, and in the very frequent infection category, it was 66% in Sebengkok. In Kampung Satu, the level of ectoparasites was 3,84, which is considered low, and 8,30, which is considered medium, in Sebengkok.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135463042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rahadinda Mutia Dhamar Drajad, Y. Nurdian, I. Astuti, W. S. Utami, Yunita Armiyanti, Bagus Hermansyah
{"title":"Correlation between Sanitation of Cow Stalls on Flies Density and Cryptosporidium sp. Manifestations","authors":"Rahadinda Mutia Dhamar Drajad, Y. Nurdian, I. Astuti, W. S. Utami, Yunita Armiyanti, Bagus Hermansyah","doi":"10.20473/jmv.vol6.iss1.2023.21-28","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss1.2023.21-28","url":null,"abstract":"The purpose of this study was to determine the correlation of stall sanitation on fly density and Cryptosporidium sp. presence on housefly. This study type was observational with a cross sectional approach in Jatian Village, Pakusari District, Jember Regency on December 2021 - February 2022. Data type was primary data that obtained using interview and observation. The correlation test that used was contingency coefficient test. The results showed that 77,5% of the stall had high fly density. Prevalence of Cryptosporidium sp. on housefly was 10%. Statistical analysis showed that cattle manure handling affected fly density (p≤0,05) with low correlation (r=0,384), meanwhile air vents affected Cryptosporidium sp. presence on housefly (p≤0,05) with low correlation (r=0,359). Farmers were suggested to keep the stall sanitation optimal to prevent Cryptosporidium transmission to humans.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90461540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seli Novitasari, Arimbi Arimbi, M. Yunita, I. S. Hamid, Prima Ayu Wibawati
{"title":"Risk Factors and Incidence of Feline Lower Urinary Tract Disease (FLUTD) in Sahabat Satwa Genteng Animal Clinic","authors":"Seli Novitasari, Arimbi Arimbi, M. Yunita, I. S. Hamid, Prima Ayu Wibawati","doi":"10.20473/jmv.vol6.iss1.2023.15-20","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss1.2023.15-20","url":null,"abstract":"The purpose of this study was to determine the risk factors and incidence of Feline Lower Urinary Tract Disease (FLUTD) at the Sahabat Satwa Genteng clinic. A total of 36 samples were evaluated according to research procedures with diagnosis, data collection, urine sampling, dipstick and sediment examination methods, and Chi-square data analysis. The results showed that there was a association between gender, history of castration, and diet on the risk of FLUTD. Meanwhile, there was no association between age and the risk of FLUTD. Clinical signs that often occur in FLUTD disease initiate from stranguria, hematuria, dysuria, periuria, and polykisuria. The results of the dipstick examination founded that 86,1% of cats affected by FLUTD had a normal pH and 13,9% had an alkaline pH. The results of the specific gravity (SG) of cats with FLUTD have values below normal while the protein levels found in all cats suffering from FLUTD have values above normal. Stranguria was found in 69,4% of cats with FLUTD. Leukocytes were also found in all the urine of cats suffering from FLUTD. The most common results of sediment examination were struvite, calcium oxalate, and cystine crystals.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84102898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Kenconojati, M. F. Ulkhaq, M. H. Azhar, Nina Rofi Rukmana
{"title":"Vibriocidal Activity of Ethanol Extract of Moringa Leaves and Its Effect on the Growth of Pacific White Shrimp","authors":"H. Kenconojati, M. F. Ulkhaq, M. H. Azhar, Nina Rofi Rukmana","doi":"10.20473/jmv.vol6.iss1.2023.75-81","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss1.2023.75-81","url":null,"abstract":"This study aimed to evaluate the vibriocidal potency of Moringa leaf ethanol extract (MLEE) and assess the effectiveness of dietary supplementation of MLEE on Pacific white shrimp growth performance. A vibriocidal activity was performed using disk diffusion and tube dilution methods. The results showed Vibrio parahaemolyticus was more susceptible than Vibrio harveyii with an inhibitory zone of 11.30 to 22.90 mm. The minimum inhibitory concentration (MIC) of MLEE against V. parahaemolyticus was recorded at 12.5%, similar to V. harveyii. The effectiveness of MLEE on diet was monitored by the growth of the shrimps for 40 days of culture. Shrimps with an average initial weight of 2.50±0.05 g were acclimatized in 10 days. The MLEE was administered to the shrimps at different concentrations i.e., 10%, 15%, 20%, and 25%. The control diet was prepared without MLEE supplementation. After 40 days of culture, the shrimps fed with 10% of MLEE had the best growth. This study reported that MLEE potentially become vibriocidal agents. However, the administration on shrimp had no beneficial effect on its growth performance.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72951171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Erprinanda Galuh Berliana, Suzanita Utama, W. Wurlina, P. Srianto, T. Lestari, H. Ratnani
{"title":"Profile of Progesterone Levels After Administration of Gonadotropins in Dairy Cattle with Ovarian Hypofunction","authors":"Erprinanda Galuh Berliana, Suzanita Utama, W. Wurlina, P. Srianto, T. Lestari, H. Ratnani","doi":"10.20473/jmv.vol6.iss1.2023.1-5","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss1.2023.1-5","url":null,"abstract":"This study aimed to evaluate the profile of progesterone in dairy cattle with ovarian hypofunction. A total of 10 cows in this study were evaluated three times in the collection phase, i.e. (F1) when the cow was diagnosed with ovarian hypofunction, (F2) when the cow was in heat and (F3) 21 days after artificial insemination to detect pregnancy. Samples in the form of blood serum were then analyzed using ELISA. As a result, the average time of heat in ovarian hypofunction cows was 7,4 days. Progesterone levels in F1 were 1,027 ng/ml, 2,774 ng/ml, 1,476 ng/ml, 2,256 ng/ml, 1,258 ng/ml, 1,758 ng/ml, 2,393 ng/ml, 0,592 ng/ml, 0,755 ng /ml, 1,876 ng/ml, respectively. Progesterone levels in F2 were 0,671 ng/ml, 0,517 ng/ml, 0,763 ng/ml, 0,598 ng/ml, 0,615 ng/ml, 0,537 ng/ml, 0,726 ng/ml, 0,643 ng/ml, 0,593 ng/ml, 0,975ng/ml, respectively. Progesterone levels in F3 were 15,642 ng/ml, 4,215 ng/ml, 17,327 ng/ml, 20,721 ng/ml, 5,796 ng/ml, 17,214 ng/ml, 15,815 ng/ml, 16,745 ng/ml, 4,632 ng /ml, 18,281 ng/ml, respectively. The pregnancy rate in hypofunctional cows treated with PG-600 in this study was 70%.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"582 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76783903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Uswatun Khasana, D. Meles, Ratih Novita Praja, W. Tyasningsih, Prima Ayu Wibawati
{"title":"Risk Factors for Feline Otitis in Madiun: A Prospective Study","authors":"Uswatun Khasana, D. Meles, Ratih Novita Praja, W. Tyasningsih, Prima Ayu Wibawati","doi":"10.20473/jmv.vol6.iss1.2023.29-34","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss1.2023.29-34","url":null,"abstract":"This study aimed to determine the risk factors in cats associated with the incidence of otitis. A total of 169 cat samples were evaluated by filling out questionnaires and interviewing owners, then an examination was carried out to determine symptoms and treatment so that appropriate action could be taken. Data were analyzed descriptively and tested using Chi-Square analysis. As a result, there were 26 (15,4%) cats diagnosed with otitis. The results of the analysis of significant risk factors include bathing frequency, ear hygiene, and maintenance patterns that can increase the incidence of otitis. The most common clinical symptoms are pruritus, head shaking and accompanied by earwax. The description of the statement about otitis concluded that as many as 70 cat owners, most of them did not understand otitis.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87315162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. F. Amrullah, Budi Utomo, Suzanita Utama, T. W. Suprayogi, T. Lestari, T. I. Restiadi, Ristaqul Husna Belgania
{"title":"Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences","authors":"M. F. Amrullah, Budi Utomo, Suzanita Utama, T. W. Suprayogi, T. Lestari, T. I. Restiadi, Ristaqul Husna Belgania","doi":"10.20473/jmv.vol6.iss1.2023.125-131","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss1.2023.125-131","url":null,"abstract":"The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85231241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Pisestyani, Indra Permana, C. Basri, D. Lukman, M. Sudarwanto
{"title":"An Evaluation of Draminski Detector as an Early Detection Tool for Subclinical Mastitis in Dairy Cattle in Pondok Ranggon Farm","authors":"H. Pisestyani, Indra Permana, C. Basri, D. Lukman, M. Sudarwanto","doi":"10.20473/jmv.vol6.iss1.2023.6-14","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss1.2023.6-14","url":null,"abstract":"Subclinical mastitis is one of the main problems in the development of dairy farming in Indonesia. Several screening test tools have been developed to support of control program for this problem. This study aimed to assess the suitability of the Draminski detector as a screening tool for subclinical mastitis in dairy cows. Quaternary milk samples from 27 dairy cows during normal lactation in the Pondok Ranggon area, East Jakarta were collected to be evaluated for the possibility of coming from quarters suffering from subclinical mastitis. Each milk sample was tested with 3 types of tests, i.e. the Breed method (golden standard) which was based on the number of somatic cells, the device Milkchecker which was based on electrical conductivity, and the Draminski detector which was based on electrical resistance. The results showed that the Draminski detector had a poor level of compatibility with the Breed method as the golden standard (Kappa value 0,013) and the Milkchecker tool (Kappa value 0,036). This test method was also considered to have poor test performance in diagnosing cases of subclinical mastitis in dairy cattle with a sensitivity value of 3,5%, specificity 100%, positive predictive value 100%, negative predictive value 18,8%, estimated prevalence 2,9%, and the true prevalence was 81,7%. In conclusion, the Draminski detector cannot be used to replace the Breed method and Milkchchecker as a screening test tool in cases of subclinical mastitis in dairy farms in Indonesia which were generally reared in the dairy farms.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90309510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fracture Treatment using External Skeletal Fixation (ESF) Method in Golden Hornbill (Rhyticeros undulates)","authors":"Muhammad Noor Rahman, Desty Apritya, D. Sari","doi":"10.20473/jmv.vol6.iss1.2023.142-144","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss1.2023.142-144","url":null,"abstract":"A golden hornbill (Rhyticeros undulates), male, one year old, reported clinical signs that one of the legs was limping. Based on the X-ray examination reported that there was a fracture of the tibiotarsus bone. External skeletal fixation (ESF) was performed for connecting bones using size 1,2 pins. The general anesthetic used was a combination of zolazepam-tiletamine at a dose of 5 mg/kgBW and given 0,1 mg/kgBW meloxicam. Postoperative therapy includes enrofloxacin at a dose of 15 mg/kg intramuscularly, dexamethasone at a dose of 0,2 mg/kgBW intramuscularly, and Osteocare® at a dose of 5 mg/kg orally. After five days postoperatively, the bird's condition had returned to normal. It can be concluded that fractures in birds can be treated using the ESF method.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89855655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}