经皮给药明日叶纳米溶体凝胶对白化大鼠烧伤创面体内愈合的影响

Akhmad Afifudin Al-Anshori, Anandia Nafisah Putri, Alif Noviana Ismi, Mohamad Kharis Suhud, Hani Plumeriastuti, Lilik Maslachah
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引用次数: 0

摘要

本研究旨在评价经皮给药木叶纳米溶酶体凝胶治疗白化大鼠烧伤创面的体内愈合过程。采用冷法制备大豆卵磷脂、胆固醇、乙醇、水和明日叶纳米颗粒。选取25只雄性大鼠,随机分为5组,(C-)不含纳米颗粒提取物组,(C+) 1%磺胺嘧啶银®组,治疗组分别用剂量为(T1) 1%、(T2) 2.5%、(T3) 5%的木叶纳米酶体凝胶处理。治疗开始于第1天或烧伤创面诱导后14天,每天2次。第15天取烧伤瘢痕组织,常规染色做组织病理学准备。光学显微镜下观察胶原蛋白、多形核细胞浸润、血管生成和再上皮化。T3组烧伤创面愈合效果最好,这可能与石竹叶纳米颗粒中含有抗氧化、抗炎和抗菌的化合物有关,说明5%石竹叶纳米凝胶经皮给药治疗大鼠皮肤烧伤创面是有效的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats
This study aimed to evaluate the in-vivo burn wound healing process in albino rats treated using transdermal delivery nano ethosomal gel from ashitaba leaves. Ethosomal vesicles were formulated using soy lecithin, cholesterol, ethanol, water, and ashitaba leaf nanoparticles using the cold method. A total of 25 male rats were randomly divided into 5 groups, i.e., (C-) treated without nanoparticle extract, (C+) treated using 1% Silver Sulfadiazine®, treatment group treated nano ethosomal gel from ashitaba leaves with the respective doses were (T1) 1%, (T2) 2,5%, and (T3) 5%. Therapy was initiated on day 1 or after being induced by a burn wound for 14 days, twice a day. On the 15th day, a termination was carried out to take skin tissue from burn scars, then histopathology preparations were made with routine staining. Microscopic observations with an optical microscope on collagen, polymorphonuclear cell infiltration, angiogenesis, and re-epithelialization. The T3 group that was treated using 5% nano ethosomal gel showed the best burn wound healing, this may be caused by compounds in ashitaba leaf nanoparticles which have antioxidant, anti-inflammatory, and antibacterial effects, thus the use of transdermal delivery therapy of 5% ashitaba leaf nano ethosomal gel was effective for wound treatment burn on rat skin.
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