Journal of Basic and Applied Research in Biomedicine最新文献_第2页

Serum Levels of Selected Cytokines in Male Nigerian Farm Workers Exposed to Dichlorvos Organophosphate Pesticide 接触敌敌畏有机磷农药的尼日利亚男性农场工人血清中选定细胞因子的水平

Journal of Basic and Applied Research in Biomedicine Pub Date : 2022-06-25 DOI: 10.51152/jbarbiomed.v8i1.209

S. Yaqub, S. Rahamon, O. Arinola

{"title":"Serum Levels of Selected Cytokines in Male Nigerian Farm Workers Exposed to Dichlorvos Organophosphate Pesticide","authors":"S. Yaqub, S. Rahamon, O. Arinola","doi":"10.51152/jbarbiomed.v8i1.209","DOIUrl":"https://doi.org/10.51152/jbarbiomed.v8i1.209","url":null,"abstract":"Background: Dichlorvos Organophosphate Pesticide (DOP) is an occupational and environmental toxicant which has been classified as highly hazardous and carcinogenic. DOP has been associated with a number of immunocompromised effects as it interferes and interacts with the specific immunological functions. However, there is lack of information on the effects of DOP on serum cytokine levels in DOP users, thus, selected pro- and anti-inflammatory cytokines were therefore measured in adult male farmer workers exposed to DOP. Materials and Methods: One hundred and twenty adult male Farm Workers (FW) consisting of 60 Pesticide Applicators (PA) and 60 farmers exposed to DOP for not less than ten years were randomly enrolled into this study. Sixty apparently healthy adult males without occupational exposure to DOP served as controls. Serum levels of pro-inflammatory cytokines [tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ)] and anti-inflammatory cytokines [interleukins (IL)-4 and IL-10] were determined using ELISA. Data were analyzed using ANOVA, Post-Hoc and Pearson correlation coefficient. P-values less than 0.05 were considered as statistically significant. Results: The serum levels of IFN-γ and IL-4 were significantly higher in FW compared with the controls while the serum levels of IFN-γ, IL-4 and IL-10 were significantly higher in PA compared with the farmers. IL-4 had significant positive correlation with IFN-ϒ in farm workers, PA and farmers exposed to DOP but IL-4 had significant positive correlation with TNF-α in farmers only. Conclusion: There is a balanced pro and anti-inflammatory cytokine secretion in farm workers with long term exposure to DOP which regulates the pathogenicity of DOP exposure.","PeriodicalId":179698,"journal":{"name":"Journal of Basic and Applied Research in Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114009094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum and Urinary Levels of Nitric Oxide and Uric Acid in Nigerian Children with Asthma 尼日利亚哮喘儿童血清和尿中一氧化氮和尿酸水平

Journal of Basic and Applied Research in Biomedicine Pub Date : 2021-09-03 DOI: 10.51152/jbarbiomed.v7i1.215

Arinola Og, Temiloluwa Olaiya, V. Edem, S. Rahamon

{"title":"Serum and Urinary Levels of Nitric Oxide and Uric Acid in Nigerian Children with Asthma","authors":"Arinola Og, Temiloluwa Olaiya, V. Edem, S. Rahamon","doi":"10.51152/jbarbiomed.v7i1.215","DOIUrl":"https://doi.org/10.51152/jbarbiomed.v7i1.215","url":null,"abstract":"Background: Asthma is associated with increased production of reactive oxygen and nitrogen species and an alteration in the levels of antioxidants activities in the lung and blood. The increased production of the superoxide anion radicals contributes to airway remodelling and disease severity. Physiologically, the effect of increased free radical generation is eliminated by corresponding activities of a network of antioxidants. Presently, there is the dearth of information on the steady-state concentrations of nitric oxide (NO) and uric acid (UA) in children with asthma. The serum and urinary levels of NO and UA in children with asthma were thus determined in this study. Methodology: Fifty children consisting of 25 children with asthma and 25 age-matched apparently healthy children without asthma were enrolled into this study. Serum and urinary levels of NO and UA were determined using standard methods. Results: Serum levels of NO and UA were significantly higher while the urinary levels of NO and UA were significantly lower in children with asthma compared with the controls. There was no significant correlation between the serum ad urinary levels of NO and UA in children with asthma. Also, gender differences were not observed in the serum and urinary levels of NO and UA in children with asthma. Conclusion: Children with asthma have elevated serum levels of NO and UA accompanied with suboptimal urinary excretion. Therefore, children with asthma might benefit from routine renal function assessment owing to damages that can result from systemic accumulation of UA with concomitant reduction in its urinary excretion.","PeriodicalId":179698,"journal":{"name":"Journal of Basic and Applied Research in Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126655655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vitro Insights into Prospect of Cnestis ferruginea Pulp Extract as an Antimicrobial Agent 铁杉果肉提取物体外抗菌研究进展

Journal of Basic and Applied Research in Biomedicine Pub Date : 2021-09-03 DOI: 10.51152/jbarbiomed.v7i1.214

L. Ajala, Oladejo Ogunlowo, M. Okafor, S. Ezem, Olayemi Mistura Ogunlowo, A. E. Adeleke

{"title":"In vitro Insights into Prospect of Cnestis ferruginea Pulp Extract as an Antimicrobial Agent","authors":"L. Ajala, Oladejo Ogunlowo, M. Okafor, S. Ezem, Olayemi Mistura Ogunlowo, A. E. Adeleke","doi":"10.51152/jbarbiomed.v7i1.214","DOIUrl":"https://doi.org/10.51152/jbarbiomed.v7i1.214","url":null,"abstract":"Sensitivity study of Cnestis ferruginea pulp extract with respect to the reference commercial antibiotics was tested against four bacteria (Staphylococcus aureus, Bacillus subtilis, Proteus mirabilis, and Klebsiella pneumoniae) and three fungi (Trichoderum rubrum, Aspergillus niger, and Candida albicans) strains isolated in man with a view to elucidating the inhibitory potency of the plant pulp’ extract using an agar well diffusion assay. The investigation discovered that the extract had 71% inhibitory activities against the microbial isolates tested either in sterilized or non-sterilized form and in the two different concentrations examined, which compared favourably with reference antibiotics used. The optimal performance of the pulp extract against the test organisms could be due to the presence of the metabolites such as alkaloids, flavonoids, steroids, saponins, and polyphenols found in ample form in the extract. This study has revealed the possible utilization of Cnestis ferrugineain the treatment of wound, urinary infections and in the management of oral-related infections.","PeriodicalId":179698,"journal":{"name":"Journal of Basic and Applied Research in Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122552176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antimicrobial Activity of Medicinal Compounds Produced by Fungal Degradation of Soybean Oil Using Lignocellulose Biomass as Surface 以木质纤维素为表面的大豆油真菌降解产生的药用化合物的抗菌活性研究

Journal of Basic and Applied Research in Biomedicine Pub Date : 2021-08-13 DOI: 10.51152/jbarbiomed.v7i1.212

B. Nyong, B. Jones, O. Ita, P. Ita

引用次数: 0

Effect of Rosemary Extract and Vitamin E on Lipid Peroxidation and the Quality During Chilling and Frozen Storage of Fried Nile Tilapia Fillets (Oreochromis niloticus) 迷迭香提取物和维生素E对尼罗罗非鱼鱼片冷藏和冷藏过程中脂质过氧化及品质的影响

Journal of Basic and Applied Research in Biomedicine Pub Date : 2021-03-18 DOI: 10.51152/JBARBIOMED.V7I1.202

I. Fouad, M. Madi, S. Lamlom, I. H. Attitalla

{"title":"Effect of Rosemary Extract and Vitamin E on Lipid Peroxidation and the Quality During Chilling and Frozen Storage of Fried Nile Tilapia Fillets (Oreochromis niloticus)","authors":"I. Fouad, M. Madi, S. Lamlom, I. H. Attitalla","doi":"10.51152/JBARBIOMED.V7I1.202","DOIUrl":"https://doi.org/10.51152/JBARBIOMED.V7I1.202","url":null,"abstract":"Antioxidants are added to fresh and processed meat to prevent lipid oxidation, retard development of off-flavors, and improve color stability. Synthetic antioxidants have been confirmed for their toxicological and carcinogenic effects. Thus, the food industry now chooses natural products over synthetic ones. This study was carried out to investigate the effect of Rosemary extract (R.E.) and vitamin E on the quality of fried fillets of Nile tilapia (Oreochromis niloticus) during chilling and frozen storage. Fillets of Nile tilapia were treated with (R.E.) 0.1%, 0.2%, 0.3% and Vitamin E 0.1% then stored for 5,10 and 15 days at 4±1°C and for three months at -18±2°C. Then chemical tests including Peroxide value (PV), Thio-barbituric acid (TBA), Tri-methylamine-nitrogen (TMA-N) and Total volatile base-nitrogen (TVB-N) were done to evaluate the preservative effect of (R.E.) and vitamin E during storage. The TBA and PV increased in all treatments due to lipid peroxidation. Results showed that TMA-N,TVB-N, value of (R.E.) and vitamin E treated samples were significantly lower than those of the control samples (P<0.05). Results of our investigation revealed that R.E. retarded oxidative changes in chilling and frozen fried fillets of Nile tilapia whereas R.E. 0.1%, 0.2% and vitamin E 0.1% were not as effective as R.E. 0.3% on oxidative stability. Best oxidation inhabitation results on chilling and frozen fried fillets of Nile tilapia was obtained when employing of R.E. The obtained results also showed that there was a significant (p<0.05) enhancement in sensory quality attributes of fried fillets of Nile tilapia; samples treated with R.E. and vitamin E. Conclusion, The tested R.E. had a high effectiveness as antioxidative and antimicrobial should be utilized for extending the shelf-life through retarded the spoilage and enhancing quality attributes of fried fillets of Nile tilapia during chilling and frozen storage.","PeriodicalId":179698,"journal":{"name":"Journal of Basic and Applied Research in Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125501502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Epidemiological Distinctive Survey Of Children With Acute Respiratory Tract Infections In Some Selected Hospitals Of Katsina Metropolis, Nigeria 尼日利亚卡齐纳市部分医院急性呼吸道感染患儿流行病学特点调查

Journal of Basic and Applied Research in Biomedicine Pub Date : 2020-12-23 DOI: 10.51152/jbarbiomed.v6i2.126

U. Shittu, Idris Zainab-L, Umma Sada, B. Aliyu

{"title":"Epidemiological Distinctive Survey Of Children With Acute Respiratory Tract Infections In Some Selected Hospitals Of Katsina Metropolis, Nigeria","authors":"U. Shittu, Idris Zainab-L, Umma Sada, B. Aliyu","doi":"10.51152/jbarbiomed.v6i2.126","DOIUrl":"https://doi.org/10.51152/jbarbiomed.v6i2.126","url":null,"abstract":"Acute respiratory tract infections (ARIs) in children under the age group of five (5) years are serious infections, which prevent the normal breathing function in the child's system. The infection usually begins as a viral infection in which it enters the child's system through the nose to the trachea (windpipe) and down to the lungs. This study aimed to identify the distinctive risk factors associated with the respiratory tract infection in which later it can lead the infection to become acute and find out the easy ways toward preventing the infections. The study was performed within the period of six (6) months during the rainy season between the periods of April to September using the human subjects under age group of five (5) years. The data analysis was done in the Bioconductor R package, statistics p-value with associated B-value were obtained from the distribution of the moderated t-statistic after the adjustment for multiple testing with a significance level of ((? 0.05) using LIMMA method. Pvclust method was also used to generate thousands of bootstrap samples by randomly sampling elements of the data and then compute graphic hierarchical clustering on each bootstrap copy. Distinctive risk factors of (ARTI) were identified, such as malnutrition (MNT), indoor air pollution (IAP), Crowdy and dirty environment (CDE), and parental education (PE) which shows significant influence on the infection. But indoor air pollution (IAP) with the highest level of significant influence to the infections. It is recommended that parents having children should be enlightened by the health personnel experts through different media communication channels and other channels in order to avoid leaving in dirty, crowdy and unventilated environment and to feed from recommended diets with their children.","PeriodicalId":179698,"journal":{"name":"Journal of Basic and Applied Research in Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131854822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Epidemiological and clinical characteristics of 2019 Novel Corona Virus Disease (COVID-19) in Balochistan, Pakistan 巴基斯坦俾路支省2019年新型冠状病毒病(COVID-19)流行病学及临床特征

Journal of Basic and Applied Research in Biomedicine Pub Date : 2020-10-05 DOI: 10.51152/JBARBIOMED.V6I2.19

Q. Ain, E. Larik, A. Saeed, Z. Khosa, M. Arif, A. Chaudhry, Tamkeen Ghafoor

{"title":"The Epidemiological and clinical characteristics of 2019 Novel Corona Virus Disease (COVID-19) in Balochistan, Pakistan","authors":"Q. Ain, E. Larik, A. Saeed, Z. Khosa, M. Arif, A. Chaudhry, Tamkeen Ghafoor","doi":"10.51152/JBARBIOMED.V6I2.19","DOIUrl":"https://doi.org/10.51152/JBARBIOMED.V6I2.19","url":null,"abstract":"Background: The unprecedented outbreak of 2019 novel coronavirus disease (COVID-19) drastically spread worldwide. The study was aimed to articulate the findings of a descriptive and clinical characteristic, also to access the potential risk factors link with the spread of COVID-19. Method: The COVID-19 cases reported through April 30, 2020, were extracted from Balochistan’s COVID-19 Electronic Dashboard. All cases from March to April 2020 were included. The results of the laboratory finding as well as data acquired through COVID-19 Dashboard were cleaned; descriptive exploratory analysis and chi-square were calculated to demonstrate association using Epi- Info. Results: A total of 1381 patient records extracted and screened. Of these, 1218 suspected cases and 1049 confirmed cases contributed to the analysis. Among confirmed cases, 77% (811) were male. The most affected age group was 60 – 69 years (attack rate 1.90/10,000), mean age was 36 ±17.11 SD with age range 2 months-94 years. District Quetta 82% (864) and district Pishin 5% (55) remained the epicenter for high attack rates. Among confirmed cases, a total of 14 deaths occurred for an overall Case fatality rate of 1.33%. The epi surge was peaked around March 19, April 5, and April 24, 2020. Among high-risk personals, those who had contact with infected individuals’ showed a high association of 97% (323/333) than without any contact 87% (221/254). Those who had travel history showed a prevalence of 86% (87/101) than in those who did not 59% (60/104) while those admitted in hospital with comorbid conditions showed a prevalence of 70% (26/37) than in those who did not 13% (6/46). Conclusion: COVID-19 epidemic has spread rapidly in the province. A series of multidimensional public health interventions, a cost-effective surveillance system, and the adoption of safe working practice, awareness sessions are necessary to control the COVID-19 outbreak. ","PeriodicalId":179698,"journal":{"name":"Journal of Basic and Applied Research in Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114944901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Markers of Oxidative Stress in the Saliva of Type 2 Diabetic Patients (Preprint) 2型糖尿病患者唾液氧化应激标志物的研究(预印本)

Journal of Basic and Applied Research in Biomedicine Pub Date : 2020-08-03 DOI: 10.2196/preprints.23156

O. Olayanju, C. Okwor, Gabriel Nku Odok Jr, N. Awah, F. Edem

{"title":"Markers of Oxidative Stress in the Saliva of Type 2 Diabetic Patients (Preprint)","authors":"O. Olayanju, C. Okwor, Gabriel Nku Odok Jr, N. Awah, F. Edem","doi":"10.2196/preprints.23156","DOIUrl":"https://doi.org/10.2196/preprints.23156","url":null,"abstract":"\u0000 BACKGROUND\u0000 Diabetes mellitus is associated with increased prevalence of oral diseases. Reactive oxygen species have been implicated in the development of these diseases. The saliva contains a balanced proportion of protective antioxidants and reactive oxygen species, disrupting this balance favours disease development. However, level of salivary markers of oxidative stress has not been sufficiently studied in the diabetics.\u0000 \u0000 \u0000 OBJECTIVE\u0000 Thus, this study aimed to measure salivary H2O2, NO and MDA in diabetic patients in comparison to non-diabetic controls.\u0000 \u0000 \u0000 METHODS\u0000 A total of 166 adults comprising of 95 Type 2 diabetic patients and 71 healthy non-diabetic controls were recruited for this study. About 3 ml of unstimulated saliva samples were collected from participants after rinsing their mouth with clean water. Levels of H2O2, NO and MDA were measured in all saliva samples using spectrophotometry. Data was analysed using t-test, logistic regression and receiver operating characteristics (ROC) with statistical significance set at p<0.05.\u0000 \u0000 \u0000 RESULTS\u0000 Salivary H2O2 (p=0.024) and NO (p=0.002) were significantly higher in the diabetic patients when compared to the healthy non-diabetic control group. Binary logistic regression showed that patients with Type 2 diabetic mellitus are more likely to have elevated salivary H2O2 (OR= 1.013; p=0.025) and NO (OR=1.016; p=0.003) levels. ROC analysis showed statistically significant performance of salivary NO levels in distinguishing between T2DM patients and healthy controls.\u0000 \u0000 \u0000 CONCLUSIONS\u0000 Higher levels of salivary H2O2 and NO could be a pointer to the high prevalence of oral diseases in diabetes mellitus. This calls for increased attention to oral health in diabetes management.\u0000","PeriodicalId":179698,"journal":{"name":"Journal of Basic and Applied Research in Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117062898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of Chlorobenzoic Acid Substitutes, Particularly, 2- Chlorobenzoic Acid by Aeromonas hydrophila 亲水气单胞菌降解氯苯甲酸代用品，特别是2-氯苯甲酸的研究

Journal of Basic and Applied Research in Biomedicine Pub Date : 2019-12-08 DOI: 10.51152/jbarbiomed.v5i2.44

K. Tarawneh, Farah AL-Quraishi, H. Qaralleh, A. Tarawneh, M. Al-limoun, K. Khleifat

{"title":"Biodegradation of Chlorobenzoic Acid Substitutes, Particularly, 2- Chlorobenzoic Acid by Aeromonas hydrophila","authors":"K. Tarawneh, Farah AL-Quraishi, H. Qaralleh, A. Tarawneh, M. Al-limoun, K. Khleifat","doi":"10.51152/jbarbiomed.v5i2.44","DOIUrl":"https://doi.org/10.51152/jbarbiomed.v5i2.44","url":null,"abstract":"Bacterium Aeromonas hydrophila (A. hydrophila) was isolated from the Petra Wastewater Treatment Plant effluent in southern Jordan. It was identified by using morphological and biochemical characteristics. A. hydrophila was found to be able of using chlorobenzoate compounds as carbon and energy source. These capabilities were with different biodegradation rates (4- chlorobenzoic acid 5µM/hr, 3,4-dichlorobenzoic acid 15.5µM/hr, 2- chlorobenzoic acid 41µM/hr and 3- chlorobenzoic acid 65µM/hr). The degradation ability was monitored through the release of chloride, disappearance of the substrate and finally the growth of bacterial cells on these substrates. A. hydrophila dioxygenases physiologically induced by chlorobenzoic acid compounds, were analyzed for both ortho or meta ring-cleavage of these aromatic compounds. Only 1, 2-dioxygenase activity was detected which suggest that the cleavage is through the ortho pathway. The best results of degradation of 2-CBA compound were obtained with 3mM substrate concentration, 25ºC, pH 7and 200µl inoculum size. The carbon sources affected the 2-CBA degradation differently from that on chloride and cell mass production. Nitrogen sources used reduced the degradation activity of the 2-CBA as well as in the chlorine release from 2-CBA. However, the nitrogen source L-proline had a slight enhancement effect on the biodegradation between the 40-80h.","PeriodicalId":179698,"journal":{"name":"Journal of Basic and Applied Research in Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131499775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Copper Chemical Form on The Growth of Pseudomonas aeruginosa Isolated from Burned Patients and on Its Cu Uptake 铜的化学形态对烧伤患者铜绿假单胞菌生长及其对铜的吸收的影响

Journal of Basic and Applied Research in Biomedicine Pub Date : 2019-11-23 DOI: 10.51152/jbarbiomed.v5i2.42

A. Tarawneh, H. Qaralleh, M. Al-limoun, K. Khleifat

{"title":"Effect of Copper Chemical Form on The Growth of Pseudomonas aeruginosa Isolated from Burned Patients and on Its Cu Uptake","authors":"A. Tarawneh, H. Qaralleh, M. Al-limoun, K. Khleifat","doi":"10.51152/jbarbiomed.v5i2.42","DOIUrl":"https://doi.org/10.51152/jbarbiomed.v5i2.42","url":null,"abstract":"The main object of this investigation was to shed light on the information on the level of Cu uptake by Pseudomonas aeruginosa that has been previously isolated from patients using Gram-negative E. aerogenes ans a Gram-positive Bacillus thuringiensis as a control for Cu uptake measurements. Cu uptake data showed that maximum Cu uptake was obtained by the Pseudomonas aeruginosa. The metal uptake was dependent on the type of biosorbent with different accumulation affinities toward the tested chemical form of the copper. Cells harvested at exponential growth phase showed slightly higher Cu uptake than at stationary phase, which reflect that the Cu uptake is metabolism dependent-process. The increasing order of affinity of the cupric chloride and cupric sulphate towards the three genera were almost constant. However, where cupric nitrate was used, the copper uptake behavior in Pseudomonas aeroginosa was not changed from those results with the other two chemical forms, whereas with E. aerogenes & B. thuringiensis, different Cu uptake behaviors were observed. In B. thuengenesis, the copper uptake rate as a function of initial Cu concentration were shown to increase with increasing the initial concentration of Cu, constantly above 320 ppm as compared with others. In Enterobacter with cupric nitrate, different bahavior was also shown in which the copper uptake decline sharply beyond the 320 ppm. These results reflect that the Cu uptake in P. aeruginosa is with different mechanisms as compared with other tested bacteria","PeriodicalId":179698,"journal":{"name":"Journal of Basic and Applied Research in Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134619416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1