Lens and eye toxicity research最新文献_第5页

Prevention of galactose cataract by pyruvate. 丙酮酸预防半乳糖性白内障。

Lens and eye toxicity research Pub Date : 1992-01-01

M Henein, P S Devamanoharan, S Ramachandran, S D Varma

引用次数: 0

Chorioretinitis caused by synthetic absorbable sutures. 由合成可吸收缝合线引起的脉络膜视网膜炎。

Lens and eye toxicity research Pub Date : 1992-01-01

T Yamanaka

引用次数: 0

Cytotoxicity of ophthalmic preservatives on human corneal epithelium. 眼用防腐剂对人角膜上皮细胞毒性的研究。

Lens and eye toxicity research Pub Date : 1992-01-01

B J Tripathi, R C Tripathi, S P Kolli

{"title":"Cytotoxicity of ophthalmic preservatives on human corneal epithelium.","authors":"B J Tripathi, R C Tripathi, S P Kolli","doi":"","DOIUrl":"","url":null,"abstract":"Because the corneal epithelium invariably encounters the full concentration of the preservative that is contained in multi-dose topical ophthalmic preparations, we investigated the cytotoxicity of several of these agents by using a sensitive model of human corneal epithelial cells in vitro. Primary cultures of epithelial cells were prepared from freshly enucleated globes. At confluence, all experimental cultures received a single dose of preservative at the concentration present in marketed formulations. The serum in the culture medium simulated the possible neutralizing effect of proteins present in the tear film in vivo. The cells were observed continuously by phase-contrast microscopy and time-lapse videomicrography for 24 hrs. Benzalkonium chloride at a concentration of 0.01% and chlorobutanol at 0.5% caused immediate cell retraction, as well as cessation of normal cytokinesis, cell movement, and mitotic activity; the epithelial cells degenerated within 2 hrs and 8 hrs, respectively. Cultures treated with chlorobutanol developed conspicuous blebs on the cell surface after 3 to 5 hrs of exposure. Thimerosal (0.001%) caused cell retraction, cessation of mitotic activity, and total cell destruction within 9 hrs. Sorbic acid (0.1% and 0.2%) greatly reduced cell movement and suppressed mitotic activity, but no cell death occurred. At concentrations of 50 ppm and 30 ppm, H2O2 instantaneously caused a marked retraction of the cells, followed by cessation of cytokinesis, cell movement, and mitosis. Retraction and death of the epithelial cells occurred within 12-24 hrs after exposure to 1 ppm H2O2 in serum-free medium. Polyquaternium ammonium chloride (0.001%) and polyaminopropyl biguanide (0.00005%) had no discernible effects on cytokinetic movement or on the mitotic activity of the epithelial cells. We relate our findings in vitro to those reported in vivo and discuss the mechanism of cytotoxicity of the various preservatives.","PeriodicalId":17964,"journal":{"name":"Lens and eye toxicity research","volume":"9 3-4","pages":"361-75"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12476248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modelling cortical cataractogenesis. XII: Supplemental vitamin A treatment reduces gamma-crystallin leakage from lenses in diabetic rats. 模拟皮质性白内障的发生。十二:补充维生素A治疗可减少糖尿病大鼠晶状体γ -晶体蛋白渗漏。

Lens and eye toxicity research Pub Date : 1992-01-01

H A Linklater, T Dzialoszynski, H L McLeod, S E Sanford, J R Trevithick

{"title":"Modelling cortical cataractogenesis. XII: Supplemental vitamin A treatment reduces gamma-crystallin leakage from lenses in diabetic rats.","authors":"H A Linklater, T Dzialoszynski, H L McLeod, S E Sanford, J R Trevithick","doi":"","DOIUrl":"","url":null,"abstract":"Normal and streptozotocin diabetic female Wistar rats were given normal diets with the following additions: 0, or 12,500 iu/kg food vitamin A (retinyl palmitate). At the end of 6 weeks, the rats were examined for weight gain or loss, general body condition, and cataracts. At sacrifice, blood was collected for measurement of serum glucose. gamma-Crystallin levels were determined in aqueous and vitreous humours using a radioimmunoassay. One lens (the right) was homogenized in 8 M guanidinium chloride for ATP analysis. In normal rats, gamma-crystallin was detected in both aqueous and vitreous humours, with a greater concentration found in the vitreous. Diabetes caused a 4-5 fold increase in gamma-crystallin in both aqueous and vitreous humours. Diabetes also led to a significant loss of body weight, and decrease in lens ATP levels. Addition of vitamin A to the diet resulted in reduction in gamma-crystallin leakage into the aqueous and vitreous humours. Vitamin A at 12,500 iu/kg food resulted in an increase in lens ATP for the diabetic rats. Neither streptozotocin diabetes nor vitamin A in the diet appeared to affect the weight of the lenses after 6 weeks. It is suggested that childhood vitamin A deficiency leading to latent fiber cell damage may be an important factor contributing to the high incidence of cataracts in the third world.","PeriodicalId":17964,"journal":{"name":"Lens and eye toxicity research","volume":"9 2","pages":"115-26"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12703669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraocular silicone oil effects on rabbit blood-retinal barrier permeability. 眼内硅油对兔血视网膜屏障通透性的影响。

Lens and eye toxicity research Pub Date : 1992-01-01

K Green, T M Slagle, M J Chaknis, L T Cheeks

{"title":"Intraocular silicone oil effects on rabbit blood-retinal barrier permeability.","authors":"K Green, T M Slagle, M J Chaknis, L T Cheeks","doi":"","DOIUrl":"","url":null,"abstract":"Both short- (1 week) and longer-term (7 week) studies have been made on a series of silicone oils to determine their effects on blood-retinal barrier permeability. About 25% of the vitreous humor was replaced with the test oil, and fluorophotometric determinations made after intravenous fluorescein at intervals after oil injection. The short-term studies revealed a uniform disturbance of the eye at 24 hours after oil injection that probably represents the physical disturbance of the eye during injection. For most oils the readings on day 4 and day 7 after oil injection revealed no difference between the experimental and the paired control eye. Adatomed 5000 cps oil and a Japanese 1000 cps oil caused more sustained aqueous humor fluorescein values over the first week. Longer-term studies, with two oils shown in prior studies on corneal endothelium to increase permeability and one oil that had no influence on endothelial permeability, revealed no effects on fluorescein penetration into any ocular compartment. It is apparent that the corneal endothelium offers a more sensitive paradigm for examining the toxicity of oils for predictive behavior of long-term exposure of the retina.","PeriodicalId":17964,"journal":{"name":"Lens and eye toxicity research","volume":"9 2","pages":"139-49"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12763369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of viscoelastics, cannulas, and irrigating solution additives in post-cataract surgery corneal edema: a brief review. 粘弹性剂、套管和冲洗液添加剂在白内障术后角膜水肿中的作用:简要回顾。

Lens and eye toxicity research Pub Date : 1992-01-01

D B Glasser, R O Schultz, R A Hyndiuk

引用次数: 0

Detection of free radicals in UV-irradiated lens by spin trapping ESR. 自旋俘获ESR法检测紫外透镜中自由基。

Lens and eye toxicity research Pub Date : 1992-01-01

J Murakami, Y Kozuka, M Okazaki, T Shiga

引用次数: 0

Reactive metabolite hypothesis for human senile cataract. 老年性白内障的反应性代谢物假说。

Lens and eye toxicity research Pub Date : 1991-01-01

R J Truscott, S G Pyne, M Manthey

引用次数: 0

The presence of Z-helical conformation in DNA of the calf lens. 小腿晶状体DNA中z -螺旋构象的存在。

Lens and eye toxicity research Pub Date : 1991-01-01

C E Gagna, J H Chen, G C Lavers, O G Mitchell, S H Zheng, L C Chen

{"title":"The presence of Z-helical conformation in DNA of the calf lens.","authors":"C E Gagna, J H Chen, G C Lavers, O G Mitchell, S H Zheng, L C Chen","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of this study was to reveal the presence of Z-helical conformation in normal crystalline lens DNA. Z-DNA antigen was prepared against poly(dG-dC).poly(dG-dC), which had been converted to the Z-helix conformation in high salt and then stabilized by bromination. Circular dichroism (CD) spectra confirmed the presence of left-handed Z-helix DNA. Antibodies to Z-DNA were raised in three rabbits immunized with brominated (Br-) poly(dG-dC).poly(dG-dC). These antibodies do not cross-react with polynucleotides in the B-helical form, but are specific to the left-handed Z-DNA conformation. DNA was isolated from three different regions of the calf lens. Anti-Z-DNA antisera, affinity purified IgG polyclonal anti-Z-DNA antibodies and monoclonal anti-Z-DNA antibodies were used as immunoprobes to detect the presence of S-DNA sequences. DNA from the cortex region of the lens reacted strongly with the anti-Z-DNA antibodies, but no binding could be observed in the DNA from the nucleus region. Digestion of lens DNA with DNase 1 dramatically decreased Z-DNA antibody binding, while RNase A and T1 treatment had no effect on Z-DNA immunoreactivity. This study has demonstrated that: (a) Z-DNA antibodies developed for our study can bind in high salt solutions (4M NaCl) to purified lens DNA sequences isolated from a variety of different calf lens cell types. By this criterion, lens DNA contains sequence determinants which may assume or are in the Z-helix conformation.","PeriodicalId":17964,"journal":{"name":"Lens and eye toxicity research","volume":"8 1","pages":"27-42"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13206011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of iris-ciliary complex on the organ cultured rabbit ocular lens. 虹膜-睫状体复合物对器官培养兔晶状体的影响。

Lens and eye toxicity research Pub Date : 1991-01-01

T K Niyogi, K Emanuel, J Parafina, M Bagchi

引用次数: 0