Laboratory medicine最新文献

Robert L. Schmidt, MD, PhD, MBA (November 17, 1952-October 25, 2023) 罗伯特-施密特（Robert L. Schmidt），医学博士、工商管理硕士（1952 年 11 月 17 日-2023 年 10 月 25 日

Laboratory medicine Pub Date : 2024-06-02 DOI: 10.1093/labmed/lmae040

Lauren N Pearson

引用次数: 0

Study of the diagnostic efficiency of anti-ZnT8 autoantibodies for type 1 diabetes in pediatric patients. 研究抗ZnT8自身抗体对儿童1型糖尿病的诊断效率。

Laboratory medicine Pub Date : 2024-05-02 DOI: 10.1093/labmed/lmad079

Sandra Fuentes-Cantero, Concepción González-Rodríguez, Carmen Rodríguez-Chacón, Raquel Galvan-Toribio, Joaquín Hermosín-Escudero, Antonio Pérez-Pérez, Antonio León-Justel

{"title":"Study of the diagnostic efficiency of anti-ZnT8 autoantibodies for type 1 diabetes in pediatric patients.","authors":"Sandra Fuentes-Cantero, Concepción González-Rodríguez, Carmen Rodríguez-Chacón, Raquel Galvan-Toribio, Joaquín Hermosín-Escudero, Antonio Pérez-Pérez, Antonio León-Justel","doi":"10.1093/labmed/lmad079","DOIUrl":"10.1093/labmed/lmad079","url":null,"abstract":"Objective: Zinc transporter 8 autoantibodies (ZNt8A) are 1 of the 4 main autoantibodies used for the diagnosis of type 1 diabetes (T1D), with glutamic acid decarboxylase autoantibodies (GADA), islet antigen-2 autoantibodies (IA-2A), and insulin autoantibodies (IAA). The objective of this study is to evaluate the diagnostic efficiency of these autoantibodies for the diagnosis of T1D in pediatric patients.Methods: A retrospective analysis of patients under 16 years of age with suspected T1D was made between June 2020 and January 2021. A total of 80 patients were included in the study, with 1 sample per patient. Subjects were classified according to diagnosis.Results: Of the subjects included in the study, 50 developed T1D. The diagnostic efficacy was IA-2A (cutoff ≥ 28 U/L) sensitivity 0.26 (95% CI: 0.14-0.38) and specificity 0.97 (95% CI: 0.79-1.0); GADA (cutoff ≥ 17 U/mL) sensitivity 0.40 (95% CI: 0.26-0.54) and specificity 0.87 (95% CI: 0.75-0.99); ZnT8A (cut off ≥ 15 U/L) sensitivity 0.62 (95% CI: 0.49-0.75) and specificity 0.97 (95% CI: 0.90-1.0). ZnT8A obtained the most significantly global diagnostic accuracy (0.75), and GADA with ZnT8A showed the highest correlation.Conclusion: The results obtained indicate a higher efficiency of anti-ZnT8 autoantibodies for the diagnosis of T1D in pediatric patients. Clinical efficiency of diabetic autoantibodies is method and assay dependent and influences combined diagnostic strategies.","PeriodicalId":17951,"journal":{"name":"Laboratory medicine","volume":" ","pages":"299-303"},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10148353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic value of pleural effusion Krebs von den Lungen-6 in malignant pleural effusion of patients with non-small cell lung cancer. Krebs-von den Lungen-6胸腔积液对癌症恶性胸腔积液的诊断价值。

Laboratory medicine Pub Date : 2024-05-02 DOI: 10.1093/labmed/lmad076

Junjun Wang, Liqun Ling, Shuhui Chen, Lunan Chou, Yumin Wang, Lijuan Hu

{"title":"Diagnostic value of pleural effusion Krebs von den Lungen-6 in malignant pleural effusion of patients with non-small cell lung cancer.","authors":"Junjun Wang, Liqun Ling, Shuhui Chen, Lunan Chou, Yumin Wang, Lijuan Hu","doi":"10.1093/labmed/lmad076","DOIUrl":"10.1093/labmed/lmad076","url":null,"abstract":"Objective: The aim of this study was to investigate the diagnostic potential of Krebs von den Lungen-6 (KL-6) in differentiating between malignant pleural effusion (MPE) induced by non-small cell lung cancer (NSCLC) and benign pleural effusion (BPE).Methods: We collected 143 pleural effusion samples from August 2018 to March 2021. The samples included 91 cases of MPE and 52 cases of BPE. The KL-6 and other indicators in pleural effusion were detected.Results: The level of pleural effusion KL-6 (pKL-6) in the MPE group was significantly higher than in the BPE group (Mann-Whitney U = 442.500, P = .000). The area under the curve (AUC) of pKL-6/pleural effusion adenosine deaminase (pADA) + pleural effusion carcinoembryonic antigen (pCEA)/pADA (AUC = 0.992) in diagnosing MPE was higher than that of pKL-6 alone (AUC = 0.903), with a sensitivity of 93.26% and specificity of 100%.Conclusion: The measurement of pKL-6 can differentiate NSCLC-induced MPE from BPE. Furthermore, the combined detection of pKL-6/pADA and pCEA/pADA can significantly improve the diagnostic efficiency for distinguishing NSCLC-induced MPE.","PeriodicalId":17951,"journal":{"name":"Laboratory medicine","volume":" ","pages":"271-276"},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9979211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of anti-squamous cell carcinoma antigen antibodies on serum squamous cell carcinoma antigen levels measured by chemiluminescent immunoassay and chemiluminescent enzyme immunoassay. 抗鳞状细胞癌抗原抗体对化学发光免疫测定法和化学发光酶免疫测定法测定的血清鳞状细胞癌抗原水平的影响。

Laboratory medicine Pub Date : 2024-05-02 DOI: 10.1093/labmed/lmad088

Chinami Oyabu, Itsuko Sato, Mari Yamamoto, Takamitsu Imanishi, Sho Sendo, Yoshihiko Yano

{"title":"Impact of anti-squamous cell carcinoma antigen antibodies on serum squamous cell carcinoma antigen levels measured by chemiluminescent immunoassay and chemiluminescent enzyme immunoassay.","authors":"Chinami Oyabu, Itsuko Sato, Mari Yamamoto, Takamitsu Imanishi, Sho Sendo, Yoshihiko Yano","doi":"10.1093/labmed/lmad088","DOIUrl":"10.1093/labmed/lmad088","url":null,"abstract":"Objective: The serum squamous cell carcinoma antigen (SCCA) level is a well-known tumor marker for squamous cell carcinoma. In this study, we examined the impact of immunoglobulin (Ig)-bound macromolecular SCCA on serum SCCA levels measured by 2 different methods.Methods: Seventy-five serum samples with an SCCA level >5.0 ng/mL as determined by a chemiluminescent immunoassay (CLIA) were also analyzed using a chemiluminescent enzyme immunoassay (CLEIA). The levels of IgG- and IgA-type anti-SCCA antibodies, which form immunoglobulins and macromolecules, respectively, were determined using an enzyme-linked immunosorbent assay. An absorption test was performed to confirm the presence of anti-SCCA antibodies.Results: The correlation coefficient between the values measured by CLEIA and CLIA was 0.768. The ratio of SCCA levels measured by CLEIA to those measured by CLIA in 14 samples with IgG-type anti-SCCA antibodies was significantly lower than that in samples without these antibodies (P < .031). Absorption tests showed that SCCA levels measured by CLIA might be falsely high in samples with IgG-type anti-SCCA antibodies, probably due to reactions with SCCA1.Conclusion: The level of SCCA as measured by CLIA and CLEIA methods correlate well, but the presence of SCCA antibodies can affect the results of the CLIA method.","PeriodicalId":17951,"journal":{"name":"Laboratory medicine","volume":" ","pages":"341-346"},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10230511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative study on outcomes of fasting vs postprandial thyroid function tests among pregnant mothers in a tertiary care setting in Sri Lanka. 斯里兰卡一家三级医疗机构对孕妇进行空腹与餐后甲状腺功能检测结果的比较研究。

Laboratory medicine Pub Date : 2024-05-02 DOI: 10.1093/labmed/lmad084

Shifaniya Banu Mohideen, Thamara Herath, Supun Manathunga

{"title":"A comparative study on outcomes of fasting vs postprandial thyroid function tests among pregnant mothers in a tertiary care setting in Sri Lanka.","authors":"Shifaniya Banu Mohideen, Thamara Herath, Supun Manathunga","doi":"10.1093/labmed/lmad084","DOIUrl":"10.1093/labmed/lmad084","url":null,"abstract":"Objective: Accurate estimation of serum thyrotropin (TSH) is crucial in the diagnosis of subclinical hypothyroidism (SCH) in pregnancy. We aimed to investigate whether there are significant differences between fasting and nonfasting thyroid function tests (TFTs) among pregnant mothers.Methods: We studied 100 pregnant mothers with previously unknown thyroid dysfunction. An equal number of participants were included in each trimester. All pregnant mothers underwent fasting and 2-hour postprandial TFTs (TSH, free T4).Results: Postprandial TSH (mean 1.01 mIU/L, SD 0.80) was significantly lower than the fasting TSH (mean 1.47 mIU/L, SD 1.18) in pregnancy (P < .01). Postprandial free T4 (mean 10.30 pmol/L, SD 2.01) was also lowered compared with fasting free T4 (mean 10.70 pmol/L, SD 1.99) in pregnancy (P < .01). The prevalence of SCH in pregnancy estimated using fasting TSH was 9.4% (SD 3%). In contrast, the prevalence was only 3.5% (SD 2%) when postprandial TSH was used.Conclusion: Compared with the fasting state, postprandial TSH demonstrates a statistically significant reduction that greatly influences the diagnosis of SCH in pregnant mothers. Therefore, we conclude that the timing of sampling for TFTs should be standardized, especially in the pregnant population.","PeriodicalId":17951,"journal":{"name":"Laboratory medicine","volume":" ","pages":"320-324"},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10148357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 6-year-old boy with an atypical liver neoplasm harboring a novel RPS6KA3 variant. 一名患有非典型肝肿瘤的 6 岁男孩，携带新型 RPS6KA3 变体。

Laboratory medicine Pub Date : 2024-05-02 DOI: 10.1093/labmed/lmad061

Daniel Bustamante, Jude Abadie

引用次数: 0

Proteomic biomarker evaluation using antibody microarrays: association between analytical methods such as microarray and ELISA. 利用抗体微阵列进行蛋白质组生物标志物评估：微阵列和 ELISA 等分析方法之间的关联。

Laboratory medicine Pub Date : 2024-05-02 DOI: 10.1093/labmed/lmad083

Nadezhda G Gumanova, Natalya L Bogdanova, Victoria A Metelskaya

{"title":"Proteomic biomarker evaluation using antibody microarrays: association between analytical methods such as microarray and ELISA.","authors":"Nadezhda G Gumanova, Natalya L Bogdanova, Victoria A Metelskaya","doi":"10.1093/labmed/lmad083","DOIUrl":"10.1093/labmed/lmad083","url":null,"abstract":"Objective: To evaluate the associations between analytical methods, such as microarray and enzyme-linked immunosorbent assay (ELISA); expedient cutoffs; and the lowest possible number of microarrays in analysis for target biomarker estimation in case-control studies.Methods: This study included 321 serum specimens, gathered in different case-control studies to test for atherosclerosis and atrial fibrillation. Among them, 48 serum specimens were analyzed using microarray technology. We used ELISA and commercial kits for confirmation of the results.Results: Three proteins-cadherin-P, neuronal nitric oxide synthase, and adenovirus fiber-were shown to have distinctly different values in the case group vs the control group. As a result, we used those proteins as the target for confirmation using our alternative analytical method. Also, these protein values represented the limiting range between the highest and lowest differences in case-control groups. The results of microarray assay were confirmed using ELISA and commercial kits in the same specimens, in which microarray profiling was performed, and also in separate large case-control groups.Conclusions: A 1.5-fold difference in the protein content, as measured using microarray technology, was shown to be sufficient for further investigation of the candidate proteins. As few as 3 microarrays were considered sufficient for perspective evaluation of the target proteins. Microarray serum profiling, therefore, provides semiquantitative determination of protein in serum.","PeriodicalId":17951,"journal":{"name":"Laboratory medicine","volume":" ","pages":"325-333"},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10206471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utility of a microRNA panel in diagnosis and prognosis of hepatitis C-associated hepatocellular carcinoma. 微RNA面板在丙型肝炎相关肝细胞癌的诊断和预后中的应用。

Laboratory medicine Pub Date : 2024-05-02 DOI: 10.1093/labmed/lmad081

Abeer Ahmed ALrefai, Sara Kamal Rizk, Ahmed Kamal Khamis, Zeinab A Kasemy, Mona Salah Eldin Habieb

{"title":"Utility of a microRNA panel in diagnosis and prognosis of hepatitis C-associated hepatocellular carcinoma.","authors":"Abeer Ahmed ALrefai, Sara Kamal Rizk, Ahmed Kamal Khamis, Zeinab A Kasemy, Mona Salah Eldin Habieb","doi":"10.1093/labmed/lmad081","DOIUrl":"10.1093/labmed/lmad081","url":null,"abstract":"Background: Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and the leading cause of cancer mortality. Various studies have linked dysregulated microRNA expression to liver cancers, but those related to viral hepatitis-related HCC are limited.Methods: We investigated the diagnostic and prognostic roles of circulating miR-331-3p, miR-23b-3p, and miR-3194-5p in EDTA-treated blood samples of 50 hepatitis C virus (HCV) HCC patients, 50 HCV cirrhotic patients, and 50 healthy controls using quantitative real-time polymerase chain reaction.Results: We found that miR-23b-3p and miR-3194-5p were significantly downregulated, whereas miR-331-3p was upregulated in HCC patients compared with controls. Also, these miRNAs were significantly dysregulated in HCC compared with cirrhotic patients. For the diagnosis of HCC, miR-331-3p and the combined miRNAs panel had the highest area under the curve (AUC), followed by miR-3194-5p. The highest AUC for differentiating metastatic from nonmetastatic patients was shown by miR-331-3p and the combined miRNAs panel, followed by miR-23b-3p. Dysregulation of miRNAs was associated with poor clinicopathological manifestations. Finally, miR-331-3P was found to be an independent risk factor for metastatic lesions in HCC.Conclusion: Overall, the assessed miR-331-3p, miR-23b-3p, and miR-3194-5p were significantly associated with poor clinicopathological features of HCC and could be used to discriminate HCV-related HCC patients from cirrhosis and differentiating metastatic from nonmetastatic patients, primarily miR-331-3p along with combined miRNAs. Moreover, miR-331-3p was found to be an independent factor for metastatic lesions.","PeriodicalId":17951,"journal":{"name":"Laboratory medicine","volume":" ","pages":"310-319"},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10200818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single laboratory evaluation of umbilical cord blood units processing methodologies for banking. 用于银行的脐带血单位处理方法的单一实验室评估。

Laboratory medicine Pub Date : 2024-05-02 DOI: 10.1093/labmed/lmad073

Francisco F Dos Santos, Letícia Nunes, Cátia Martins, Margaret Ann Smith, Carla Cardoso

{"title":"Single laboratory evaluation of umbilical cord blood units processing methodologies for banking.","authors":"Francisco F Dos Santos, Letícia Nunes, Cátia Martins, Margaret Ann Smith, Carla Cardoso","doi":"10.1093/labmed/lmad073","DOIUrl":"10.1093/labmed/lmad073","url":null,"abstract":"Objective: To compare the efficiency of 3 different processing methods (Sepax, AutoXpress [AXP], and manual processing with hydroxyethyl starch [HES] sedimentation) used at Stemlab during a 10-year period.Methods: Historical data were compiled and the analytical results obtained for the 3 different methods were compared.Results: The manual processing (HES) method yielded the highest level of total nucleated cell recovery after processing, and the AXP system yielded the highest CD34+ cell number. The red blood cell reduction was also significantly higher with the HES method. Also, HES showed comparable results to Toticyte technology for umbilical cord blood (UCB) processing.Conclusion: These results show that the HES method is as effective as automated technologies for UCB volume reduction; hence, it is a suitable methodology for private and public UCB banks. The HES method also proved to be superior to Toticyte technology for medical applications, with higher recovery yields of total nucleated cells after thawing and equivalent CD34+ cell recovery and functionality.","PeriodicalId":17951,"journal":{"name":"Laboratory medicine","volume":" ","pages":"285-292"},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10351051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective comparison of false-positive result frequencies of 3 syphilis serology screening tests in pregnant and nonpregnant patients at an academic medical center in Appalachia. 回顾性比较阿巴拉契亚地区一家学术医疗中心对怀孕和未怀孕患者进行的 3 种梅毒血清学筛查试验的假阳性结果频率。

Laboratory medicine Pub Date : 2024-05-02 DOI: 10.1093/labmed/lmad078

Jianbo Yang, Danyel H Tacker, Sijin Wen, P Rocco LaSala

{"title":"Retrospective comparison of false-positive result frequencies of 3 syphilis serology screening tests in pregnant and nonpregnant patients at an academic medical center in Appalachia.","authors":"Jianbo Yang, Danyel H Tacker, Sijin Wen, P Rocco LaSala","doi":"10.1093/labmed/lmad078","DOIUrl":"10.1093/labmed/lmad078","url":null,"abstract":"Objective: This study retrospectively compared false-positive result frequencies of 3 syphilis serology screening tests and assessed whether false positivity was associated with pregnancy and age.Methods: Results for 3 screening tests were retrieved from the laboratory database, including rapid plasma reagin (RPR) assay between October 2016 and September 2019, BioPlex 2200 Syphilis Total immunoassay between May 2020 and January 2022, and Alinity i Syphilis TP assay between February 2022 and April 2023. The false-positive result frequencies were calculated based on testing algorithm criteria.Results: False-positive result frequency for BioPlex was 0.61% (90/14,707), significantly higher than 0.29% (50/17,447) for RPR and 0.38% (55/14,631) for Alinity (both P < .01). Patients with false-positive results were significantly older than patients with nonreactive results for RPR (median age: 36 vs 28, P < .001), but not for BioPlex or Alinity. For all 3 tests, the positive predictive values in pregnant women were lower than those in nonpregnant women or men. However, pregnant women did not exhibit a higher false-positive result frequency.Conclusion: Although false-positive result frequencies were low overall for all 3 syphilis serology tests, there is a significant difference between different tests. Pregnancy was not associated with more false-positive results for all 3 tests.","PeriodicalId":17951,"journal":{"name":"Laboratory medicine","volume":" ","pages":"293-298"},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10107942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0