Kufa Journal For Veterinary Medical Sciences最新文献

{"title":"Field Qualitative Detection of Avian Influenza Using Antigen Rapid Test in Different Districts of Nineveh Governorate, Iraq","authors":"Basila Ibrahim Hussein, Omer Ra’ad Al-Hyalee","doi":"10.36326/kjvs/2019/v10i13319","DOIUrl":"https://doi.org/10.36326/kjvs/2019/v10i13319","url":null,"abstract":"Sensitive and rapid diagnostic kits for avian influenza virus are required to screen large numbers of samples during a disease outbreak and to prevent the spread of infection. The aim of study is to investigate on avian influenza virus type A serotype H5 among different avian species in different district of Nineveh Governorate, Iraq using Anigen rapid H5 AIV Ag test kit. In this study, we employed One-step avian influenza virus type A and serotype H5 antigen test (Anigen rapid H5 AIV Ag test kit). The test was run out in different districts of Nineveh governorate, including broiler farms local poultry markets, and house poultry breedes (local breeds, broiler, and layers) as well as pigeon houses. Of tested 686 swab from total 241477 poultry samples were been tested, no avian influenza virus positive cases result were noted or confirmed by rapid screening test. No clinical signs were recorded in all examined poultry species. The study conclude that kit used for rapid diagnosis of avian influenza type A serotype H5 was easy, reliable and its interpretation less than thirty minutes.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88027687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection Of Intracellular Adhesion Gene (Icaa And Icad) And Biofilm Formation Staphylococcus Aureus Isolates From Mastitis Milk Of Cow","authors":"Hasan I. Idbeis, Mohammed H. Khudor","doi":"10.36326/kjvs/2019/v10i13317","DOIUrl":"https://doi.org/10.36326/kjvs/2019/v10i13317","url":null,"abstract":"In the present study, a total of 75 mastitis milk samples were collected from cow and were analyzed for the presence of S.aureus. The obtained results indicate that this bacterium observed in 26.66%. The study of antibiotic susceptibility test to 9 different antibiotics showed that S. aureus was 100% resistant to penicillin and 100% sensitive to Vancomycin, Gentamycin , Clarithromycin and Chloramphenicol . Whereas for cefoxitin (alternative to methicillin) resistance was 50%.There were a variable resistance percentage for the rest of antibiotics: Tetracycline (30%), Ciprofloxacin and Clindamycin (22%). The biofilm-forming ability of S. aureus was evaluated via microtiter plates and the result revealed that, all the studied isolates were either moderate biofilm producer or weak biofilm producer while the non-biofilm producer and strong biofilm producer were not detected among the tested isolate. PCR analysis was applied to DNA extracted from S.aureus isolates from milk samples .The results of PCR assay revealed that all S.aureus isolates gave positive results for both icaA and icaD genes (100%) with Product size 151 and 211 bp, respectively. \u0000Results of this study indicate that biofilm producing S.aureus have a major role player on the occurrence of bovine mastitis in addition, there were high prevalence of MRSA isolates (50%) in mastitis milk at the study area.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74888390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Silymarin on Oxidative Stress Biomarker, Lipid Peroxidation and Reproductive Function of Diabetic Albino Rats","authors":"Nabeel Abed, Fouad Zaidan Hamza, R. S. Nuhair","doi":"10.36326/kjvs/2019/v10i13327","DOIUrl":"https://doi.org/10.36326/kjvs/2019/v10i13327","url":null,"abstract":"The present study was carried out in the animal house and laboratories of the College of Veterinary Medicine/ University of Basrah to investigate the oral administration of silymarin on oxidative stress biomarker, lipid peroxidation and reproductive function in alloxan induced diabetic of albino rats. After the period of acclimation, 32 male rats were divided into 4 equal groups with 8 animals in each as the following: Control group: Animals of this group administered distilled water, Silymarin group: administered silymarin (20mg/kg) dissolved in distill water orally by gavages. Alloxan treated (DM) group : were given alloxan intraperitoneal injection 150mg/BW, as single dose for induced diabetic mellitus and Alloxan + silmarin treated group : were injection alloxan intraperitonealy and then after 3 days, oral administered of (20 mg/kg bw ) silymarin . The experimental was continued for 45 day. At the end of the experiment, animals of each group were sacrificed, and blood samples were obtained for oxidative stress biomarker, Lipid Peroxidation and hormonal assessment and the caudal epididymis was then separated from the testicles for the evaluation of epididymal sperm characteristics, while the testes were kept in 10% neutral buffer-formalin for histopathological study. The results showed a significant decrease (P ≤ 0.05) in serum superoxide dismutase (SOD) , Glutathione Peroxidase (GPX) , follicle stimulating hormone (FSH), and luteinizing hormone (LH) and testosterone, concentrations of DM group. On the other hand a significant decrease (P ≤ 0.05) in epididymal sperm concentration, individual sperm motility, live sperm percentage and a significant increase in malondialdehyde (MDA) and sperm abnormalities percentage were recorded in DM group. Histopathological examination of the testes revealed marked suppression of spermatogenesis with absence of mature spermatocytes of DM group. Moreover DM groups treated with Silymarine showed normal oxidative biomarkers, hormonal concentration, epididymal sperm characteristics, and normal testicular tissues compare with control group.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89400929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of dapagliflozin on serum electrolytes concentration in adult male rats","authors":"M. S. AL‑Mozie'l, Waleed K. Ghanim, N. Al-Shawi","doi":"10.36326/kjvs/2018/v9i24166","DOIUrl":"https://doi.org/10.36326/kjvs/2018/v9i24166","url":null,"abstract":"Hyperkalemia hazard is increased in diabetes, especially in patients with renal disease or those receiving angiotensin-converting enzyme (ACE) medications, angiotensin receptor blockers (ARBs) or potassium-sparing diuretics. Also, other diuretics can increase hypokalemia danger. Recently, We assessed  the effects of dapagliflozin in ameliorate the serum calcium, sodium, potassium( Ca+2, Na+2, K+ ) and uric acid concentration in  adults male  rats.  In this study were erolled 18  adult male rats  randomly  assigned to  three groups(n=6) as following: control group (I)  0.5 ml/kg B.W  of normal saline. Group II and III receive orally by gavage  for 30 days  0.53 mg/kg and 1.06 mg/kg B.W of dapagliflozin.  The end of experiment the blood were collected for measuring  the following parameter: blood glucose,  Ca+2, Na+2, K+ ) , and  uric acid concentration. The results of the present study revealed the following: there was significant  (P≤0.05) increase in body weight,  K+, Ca+2 and uric acid concentration of  treated II, and III groups compared with control. On the other hand, there was significant  (P≤0.05) decrease in Na+2  concentration of treated compared with control. The experiment was  concluded that   Dapagliflozin in dose dependent pattern produce significant effects in reduction body weight in II and III groups compared with  I group, furthermore, shows that changes in serum concentrations  of potassium, calcium and uric acid in treated groups compared to normal where as no effects on sodium concentration was found. Also there is no differences in blood glucose concentration in treated group compared to normal, rather than hypoglycemic and all groups are normal groups.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84040155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of CoQ10 on Some Biochemical and Histological Aspects of Kidney in Rats Exposed to Sodium Fluoride","authors":"B. N. Al-Okaily, I. Mohammed","doi":"10.36326/kjvs/2018/v9i24171","DOIUrl":"https://doi.org/10.36326/kjvs/2018/v9i24171","url":null,"abstract":"This experiment was aimed to investigate the role of CoQ10 to alleviating the functional alterations of kidney - induced by sodium fluoride (NaF) in male rats. Twenty adult male Wister albino rats were randomly assigned into four  equal groups and treated for 42 days daily as follows: first group was received drinking tap water and intubated with DMSO 1% and serving as control (group C); second group ( group G1) received sodium fluoride 100ppm in drinking tap water ; third group (group G2) received sodium fluoride 100ppm in drinking tap water plus intubated 10 mg /Kg. B.W of CoQ10 orally , whereas fourth group ( group G3) intubated 10 mg /Kg. B.W of CoQ10 only. Fasting blood samples were collected at 0, 21, and 42 days of experimental periods. Then sera was isolated for estimated serum creatinine, blood urea nitrogen (BUN) and peroxynitrite radicals concentrations, as well as catalase activities has been measured. At the end of the experiment the animals were sacrificed and  sections from the kidney were taken for histopathological examination. The results revealed that rats received NaF (group G1)  for 42 days caused significant decrease in body weight and serum catalase activity , while a significant increase in the concentrations of serum creatinine, blood urea nitrogen (BUN) and peroxynitrite radicals accompanied with histopathological alterations of kidney were showed as compared to other experimental groups . On the other hand, administration of   CoQ10  to groups G2 showed an improvement of physiological and histological alterations and keeping the  kidney functions around the normal levels, manifested its protective effects against deleterious  effects of NaF. It could be concluded that  these results confirmed the antioxidant properties of CoQ10 against nephrotoxicity induced by NaF in adult rats.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80270275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attenuation of Ifosfamide Adverse Effect on Leydig, Sertoli and Spermatogonia Cells of Ram Testis by Liposome Technique in vitro","authors":"M. Al-Bayati, Laith H. Al-Salhi","doi":"10.36326/kjvs/2018/v9i24162","DOIUrl":"https://doi.org/10.36326/kjvs/2018/v9i24162","url":null,"abstract":"Attenuation of Ifosfamide Adverse Effect on Leydig, Sertoli and Spermatogonia Cells of Ram Testis by Liposome Technique in vitro","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78004028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of the Electrometric Method in Measuring the Activity of Cholinesterase in Local Female Buffalo in north of Iraq","authors":"A. S. Alias","doi":"10.36326/kjvs/2018/v9i24169","DOIUrl":"https://doi.org/10.36326/kjvs/2018/v9i24169","url":null,"abstract":"The aim of present study, was desigen to measurement of cholinesterase (ChE) activity by electrometric method in normal blood of adult Female buffalo, Cholinesterase was used in red blood cells (RBCs)to determine the linear relationship between incubation time and cholinesterase activity. The change in pH of RBCs cells reaction mixture (0.21 to 0.47) agreed with increased incubation time of 10 - 50 minutes and the pH change red blood cells reaction mixture (0.31 to 0.46) was associated with increased incubation temperature of 25-40C. recorded the highest activity of natural ChE the change in pH / 40 C° minutes in the red blood cells (0.46), whole blood (0.42) and least in the blood plasma (0.076). To illustrate the accuracy of the way the coefficient of variation in red blood cells and blood plasma and the whole blood (1.65%, 6.97% and 4.12%), respectively, this was done using 0.1 ml acetylcholine iodide 7.5% as the basis of the combination interaction of the current study. Quinidine sulfate specifically inhibited pseudo ChE in the RBC and whole blood, and it was estimated to be (6.5%,16.7%), where as true ChE activity was (93.5%, 83.3%).  Monocrotophos at concentration (0.5, 1 µM) and carbaryl at (5, 10 µM)  significantly inhibited RBCs ChE in vitro.  These results indicate that the used of modified electrometric method its simple, precise, efficient and have the validity for measurement of ChE activities in adult Female of buffalo.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89953732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some Epidemiological Aspects of Piroplasmosis of Sheep and Camels in desert of Al-najaf","authors":"A. Al-mialy, A. A. Hatem, Abdul Hadi Jaythoom AL-Abedi, Ismaeel Raheem Jabbar, Z. Ali","doi":"10.36326/kjvs/2018/v9i24170","DOIUrl":"https://doi.org/10.36326/kjvs/2018/v9i24170","url":null,"abstract":"This project attends to exam  the prevalence of piroplasmosis in sheep and camels in Al-Najaf desert of Iraq during period of May 2015 to April 2016. The samples were randomly collected from different regions in Al-Najaf province and represent 230 sheep (127 female, 103 male ) and 140 camels ( 90 shecamels and 50 male) . Blood smears were subjected to Geimsa stained and microscopic  examination. It was observed that prevalence of babesiosis and theileriosis in sheep were 26.9% and 12.17% respectively ,while in camels the prevalence was25% for babesiosis and 10.7% for theileriosis . The highest incidence for babesiosis was recorded in camels at age more than 4 years , while the lowest percentage was recorded in ages between6-12 month in both species. The highest ratio for theileriosis was observed in camels at ages 1-4 years.The maximal occurrence of babesiosis and theileriosis were noticed in summer season  (33.5%) and (15%) respectively in both species , while in winter season were (17.6%)and ((7.6%)respectively .The highest percentage of babesiosis was found in females of sheep and camels (41.7%and 30%respectively) than male (18.4%and 16%respectively).","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87482541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological study of effects phenobarbital in Goats","authors":"Zainab W. K, Hanan Ali, S. A. Al-zahra","doi":"10.36326/kjvs/2018/v9i24168","DOIUrl":"https://doi.org/10.36326/kjvs/2018/v9i24168","url":null,"abstract":"In this data was done to demonstrate the administration of phenobarbital compounds & showed histopathological effects. In this study fourteen of local goats were divided into two groups (each group seven animals) for 60 days. First one were treated phenobarbital injected with 35 mg kg.B.W.,twice daily ,second group act as control group. These animals were examined internally and externally during adaptation period. After end experimental period scarified the animals, necropsy, preparation of slid by routine technique  and examined by light microscope, there are showed different histological changes into tissue of animals treatment with phenobarbital such as degeneration of epithelial cells lining of renal tubules of kidney and thyroid gland congested of central vein with fibrosis in liver ,vacuolated of mucosal layer of rumen, small intestine and pancreas, atrophyseminiferous tubules of testis and lymph node.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90749426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Humoral Immunity to Infectious Bronchitis and Newcastle Disease Vaccines and Received Artemisia herba alba Extract in layer hens","authors":"Tahseen. A. AL- Saeedi, Fadhl A. Mahmood, Weam. A. Hamad","doi":"10.36326/kjvs/2018/v9i24172","DOIUrl":"https://doi.org/10.36326/kjvs/2018/v9i24172","url":null,"abstract":"The present study aims to evaluate the effect vaccination and oral administration of aqueous extract of artemisia herba alba on the immune status. forty laying hens (Lohman Brown,1 year-old, body weight 1.8 kg) were divided into four groups (ten) individuals. First one is the control; the second group treated with vaccine against NDV and IBV; third group received  2ml/bird/day  5% extracts of artemisia herba alba; fourth group treated with 2ml/bird/day 5% extracts of artemisia herba alba and vaccinated against NDV and IBV. Antibody titers against NDV and IBV were measured after 14 and 28 days. Results present significant increase in the globulin of the fourth group as compared to the others. The level of NDV antibodies increases significantly in this group as compared with control and third groups after 14 days. The same group shows a significant increase at 28 days as compared with the control one only. On the other hand, antibody titer of  IBV increased significantly in the second and the fourth groups compared to the control group at 28 days, but no significant differences among all groups after 14 days of the experiment.In conclusion, this study indicates a limited positive effect of the herb extract on humoral immunity of layer chickens.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78289078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}