Korean Journal of Poultry Science最新文献

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A Survey on Egg Laying Performance and Distribution Status of Animal Welfare Certified Farms for Laying Hens 动物福利认证养殖场蛋鸡产蛋性能及分布状况调查
Korean Journal of Poultry Science Pub Date : 2019-06-01 DOI: 10.5536/KJPS.2019.46.2.55
E. Hong, H. Kang, K. Park, Jin-Joo Jeon, Hyunsoo Kim, Chan-ho Kim, Sang-Ho Kim
{"title":"A Survey on Egg Laying Performance and Distribution Status of\u0000 Animal Welfare Certified Farms for Laying Hens","authors":"E. Hong, H. Kang, K. Park, Jin-Joo Jeon, Hyunsoo Kim, Chan-ho Kim, Sang-Ho Kim","doi":"10.5536/KJPS.2019.46.2.55","DOIUrl":"https://doi.org/10.5536/KJPS.2019.46.2.55","url":null,"abstract":"This study was conducted to evaluate animal welfare approved farms in three housing systems (open, windowless, and free-range). The survey was conducted in 25 animal welfare approved farms, and 10 farms were surveyed for distribution status. The main breed in all animal welfare approved farms of laying hens was Hy-Line Brown variety. In the case of open house, laying hens were bred in traditional and panel houses simultaneously; however, the ratio of panel house was 58.3%, which was higher than that of the traditional house. All the windowless houses were made of panels and more than 15,000 laying hens were housed in a single windowless house. In the case of free-range house, it was maintained on a small scale of less than 12,000 birds. Fifty-six percent of the surveyed farms were breeding at 7∼8 birds/m. In terms of male and female ratios, most farms maintained 1 male:15 females, but there was a farmhouse that switched 17 or 20 females to 1 male. The daily dietary allowance was 110∼170 g, and 32% of the surveyed farms provided feed of more than 150 g/day, which showed that forage feed was important. The age of at the first egg was 123 days, 122 days, and 120 days, and the peak percent was 91.8%, 94.9%, and 86.5% in open, windowless and free-range houses, respectively. The average egg production rate was 74.0%, 84.6%, and 72.7% in open, windowless, and free-range houses respectively, thus, there was no correlation between feed intake and hen-housed eggs. Distribution of welfare certified eggs was mainly a direct deal with the consumer or through contract production. The ratio of direct transactions between large-scale marts and eco-friendly specialty stores of welfare approved eggs was higher than that of conventional eggs. The rate of contract sales of eggs in both the barn and free-range systems was high, and the percentage of courier sales farms was also high. Excluding courier services, price of eggs in the barn system rose to more than 30 won/egg in the second half of 2017 (after AI). Price of eggs in the free-range system rose to more than 50 won/egg in the second half of 2017 (after AI). In the case of courier sales, the same price of 500 won was maintained before and after AI. In conclusion, the results of this study can be used as basic data for improving the animal welfare certification system for laying hens in Korea. (","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83740400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quality Characteristics of Samgyetang with Medicinal Herbs 中药参鸡汤的品质特征
Korean Journal of Poultry Science Pub Date : 2019-06-01 DOI: 10.5536/KJPS.2019.46.2.95
Samooel Jung, Tae-Kyung Kim, Su-Kyung Ku, H. Yong, Kyung-Woo Lee, Young-Boong Kim, Yun-Sang Choi
{"title":"Quality Characteristics of Samgyetang with Medicinal Herbs","authors":"Samooel Jung, Tae-Kyung Kim, Su-Kyung Ku, H. Yong, Kyung-Woo Lee, Young-Boong Kim, Yun-Sang Choi","doi":"10.5536/KJPS.2019.46.2.95","DOIUrl":"https://doi.org/10.5536/KJPS.2019.46.2.95","url":null,"abstract":"This study investigated the effect of traditional medicinal plants on the quality characteristics of Samgyetang breast meat and broth. The traditional medicinal plants used in this study were roots of Panax ginseng, Codonopsis lanceolata, Platycodon grandiflorum, Salvia miltiorrhiza, Adenophora triphylla, and Allium hookeri. There was no significant difference (P>0.05) in the moisture, protein, ash content, water holding capacity, and cooking loss of Samgyetang meat with the addition of traditional medicinal plants. The color values (lightness, redness, and yellowness) of Samgyetang meat and broth were significantly different (P<0.05); the 2-thiobarbituric acid reactive substances and shear force of Samgyetang meat were significantly different (P<0.05); and the pH, turbidity, and viscosity of Samgyetang broth were also significantly different (P<0.05) with the addition of traditional medicinal plants. In the overall acceptability of Samgyetang meat, the addition of S. miltiorrhiza showed the highest score compared to that of other treatments (P<0.05). Thus, the addition of traditional medicinal plants in the production of Samgyetang were found to affect the lipid rancidity beneficially with plant species when compared to Samgyetang with Panax ginseng. In conclusion, with the development of Samgyetang using S. miltiorrhiza, it will be possible to develop products with superior quality characteristics in antioxidant, shear force, and overall acceptability. (","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83331364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic Diversity of Korean Native Chicken Populations in DAD-IS Database Using 25 Microsatellite Markers 利用25个微卫星标记分析DAD-IS数据库中韩国地方鸡群体的遗传多样性
Korean Journal of Poultry Science Pub Date : 2019-06-01 DOI: 10.5536/KJPS.2019.46.2.65
H. Roh, Kwan-Woo Kim, Jinwook Lee, D. Jeon, Seungchang Kim, Y. Ko, Seong-Sil Mun, Hyunjoo Lee, Jun-Heon Lee, D. Oh, Jae-Hyun Byeon, C. Cho
{"title":"Genetic Diversity of Korean Native Chicken Populations in DAD-IS\u0000 Database Using 25 Microsatellite Markers","authors":"H. Roh, Kwan-Woo Kim, Jinwook Lee, D. Jeon, Seungchang Kim, Y. Ko, Seong-Sil Mun, Hyunjoo Lee, Jun-Heon Lee, D. Oh, Jae-Hyun Byeon, C. Cho","doi":"10.5536/KJPS.2019.46.2.65","DOIUrl":"https://doi.org/10.5536/KJPS.2019.46.2.65","url":null,"abstract":"A number of Korean native chicken(KNC) populations were registered in FAO (Food and Agriculture Organization) DAD-IS (Domestic Animal Diversity Information Systems, http://www.fao.org/dad-is). But there is a lack of scientific basis to prove that they are unique population of Korea. For this reason, this study was conducted to prove KNC’s uniqueness using 25 Microsatellite markers. A total of 548 chickens from 11 KNC populations (KNG, KNB, KNR, KNW, KNY, KNO, HIC, HYD, HBC, JJC, LTC) and 7 introduced populations (ARA: Araucana, RRC and RRD: Rhode Island Red C and D, LGF and LGK: White Leghorn F and K, COS and COH: Cornish brown and Cornish black) were used. Allele size per locus was decided using GeneMapper Software (v 5.0). A total of 195 alleles were observed and the range was 3 to 14 per locus. The MNA, Hexp, Hobs, PIC value within population were the highest in KNY (4.60, 0.627, 0.648, 0.563 respectively) and the lowest in HYD (1.84, 0.297, 0.286, 0.236 respectively). The results of genetic uniformity analysis suggested 15 cluster (ΔK = 66.22). Excluding JJC, the others were grouped in certain cluster with high genetic uniformity. JJC was not grouped in certain cluster but grouped in cluster 2 (44.3%), cluster 3 (17.7%) and cluster8 (19.1%). As a results of this study, we can secure a scientific basis about KNC’s uniqueness and these results can be use to basic data for the genetic evaluation and management of KNC breeds. (","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86356877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Effect of Hatching and Brooding Season of Chicks on Their Heat Stress Response and Production Performances 孵蛋季节对雏鸡热应激反应及生产性能的影响
Korean Journal of Poultry Science Pub Date : 2019-06-01 DOI: 10.5536/kjps.2019.46.2.77
E. Cho, E. Choi, S. Sohn
{"title":"Effect of Hatching and Brooding Season of Chicks on Their Heat Stress Response and Production Performances","authors":"E. Cho, E. Choi, S. Sohn","doi":"10.5536/kjps.2019.46.2.77","DOIUrl":"https://doi.org/10.5536/kjps.2019.46.2.77","url":null,"abstract":"This study was conducted to compare the heat stress response and production performance of chicks hatched in winter and summer. Among the 2,090 Korean native chickens examined, 1,156 hatched in winter and 934 hatched in summer. The amount of telomeric DNA, the expression of heat shock protein (HSP) genes, survival rate, egg production, and body weight were analyzed to evaluate the stress response and production performance of chickens. The results showed that the expression of HSP-70, HSP-90α, and HSP-90β genes in the winter-hatched chickens were significantly higher than those in the summer-hatched chickens during the growing and laying period (P<0.05). There was no significant difference in the amount of telomeric DNA between summerand winter-hatched chickens. The survival rate was significantly higher in the summer-hatched chickens than in the winter-hatched chickens at the laying period (P<0.01). The hen-day egg production and egg weight in the summer-hatched chickens were also significantly higher than those in the winter-hatched chickens (P<0.05). In contrast, age of sexual maturity of winter-hatched chickens was significantly earlier than that of summer-hatched chickens (P<0.01). The body weights from birth to 24 weeks were significantly lighter in the summer-hatched chickens than in the winter-hatched chickens, however, it was reversed after 28 weeks (P<0.05). In conclusion, the chicks hatched in the summer are more resistant to heat stress, with better productivity than the chicks hatched in the winter. These results suggest that the chicks grown at high temperatures have greater adaptability to the thermal environment. (","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91253671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Multiple Genotypes of Avian Infectious Bronchitis Virus Circulating in Vietnam 越南流行的禽传染性支气管炎病毒的多基因型
Korean Journal of Poultry Science Pub Date : 2019-06-01 DOI: 10.5536/kjps.2019.46.2.127
T. Le, H. Lee, V. Le, Kang-Seuk Choi
{"title":"Multiple Genotypes of Avian Infectious Bronchitis Virus Circulating in Vietnam","authors":"T. Le, H. Lee, V. Le, Kang-Seuk Choi","doi":"10.5536/kjps.2019.46.2.127","DOIUrl":"https://doi.org/10.5536/kjps.2019.46.2.127","url":null,"abstract":"Three strains of infectious bronchitis viruses (IBVs), designated VNUA3, VNUA8 and VNUA11, were isolated from diseased/infected chickens in Hanoi, Thainguyen, and Haiphong provinces of Vietnam. These birds had received a live IBV vaccination but still suffered from infectious bronchitis. VNUA3, VNUA8 and VNUA11 harbor cleavage sites (RRTGR, HRRRR, and HRRKR, respectively) within the S protein. A BLASTN search revealed that the S gene of VNUA3, VNUA8, and VNUA11 showed the highest nucleotide identity with those of IBV strains CK/Italy/I2022/13, CK/CH/LHLJ/08-6, and GX-NN120084, respectively. Phylogenetic analyses based on the S gene nucleotide sequences revealed that VNUA3, VNUA8 and VNUA11 clustered with Q1-like, QX-like and TC07-2-like genotypes, respectively, and were closely related to reference IBV strains from China. However, the Vietnam IBVs showed high divergence from vaccine strains 4/91 and Ma5, which are used in the Vietnamese farms from which the isolates were obtained. Taken together, these results indicate that at least three genotypes of IBV are circulating among chickens in North Vietnam. This is the first report of the molecular epidemiology of IBV in Vietnam. (","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76263683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Effect of Persimmon Peel as an Additional Feeding 柿皮补饲的效果
Korean Journal of Poultry Science Pub Date : 2019-06-01 DOI: 10.5536/KJPS.2019.46.2.87
Sang-Hee Shin, Junkoo Yi, C. Kong, W. Kwon, Z. Ryoo, Eungyung Kim, Hyeonjin Kim, J. Ha, Doyoung Hong, D. Yoon, Sunghyun Kim, M. Kim
{"title":"Effect of Persimmon Peel as an Additional Feeding","authors":"Sang-Hee Shin, Junkoo Yi, C. Kong, W. Kwon, Z. Ryoo, Eungyung Kim, Hyeonjin Kim, J. Ha, Doyoung Hong, D. Yoon, Sunghyun Kim, M. Kim","doi":"10.5536/KJPS.2019.46.2.87","DOIUrl":"https://doi.org/10.5536/KJPS.2019.46.2.87","url":null,"abstract":"Huge amount of persimmon peels, which are byproducts usually arising after making dried persimmons from fresh persimmons, are generated in fall, every year. As the dry matter of persimmon peel contains carbohydrates over 80%, it could be a good candidate for feed. In this study, we evaluated the persimmon peel as a feed resource for a hen laying eggs. Persimmon peel has lower ratio of crude protein and fat composition while higher ratio of crude fiber composition compared to a basal laying hen feed. Feeding the persimmon peel by adding to the feed at the level of 2% or 5% did not affect the egg-laying rate from beginning to the peak of laying. In addition, feeding the persimmon peel did not affect the egg quality either, as the pH, Haugh unit, yolk color and shell thickness of the egg were not different from each other. The composition of amino acids and fatty acids were also almost same for each other, except for the linolenic acid which was slightly higher in the persimmon peel feeding groups. In conclusion, addition of persimmon peel to the feed up to 5% might be helpful to increase the income and reduce the waste produced from the agricultural byproduct. (","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89266071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production Performance of 12 Korean Domestic Chicken Varieties Preserved as National Genetic Resources 作为国家遗传资源保存的12个韩国家鸡品种的生产性能
Korean Journal of Poultry Science Pub Date : 2019-06-01 DOI: 10.5536/KJPS.2019.46.2.105
K. Kim, E. Choi, J. Kwon, Hyun Chul Jung, S. Sohn
{"title":"Production Performance of 12 Korean Domestic Chicken Varieties\u0000 Preserved as National Genetic Resources","authors":"K. Kim, E. Choi, J. Kwon, Hyun Chul Jung, S. Sohn","doi":"10.5536/KJPS.2019.46.2.105","DOIUrl":"https://doi.org/10.5536/KJPS.2019.46.2.105","url":null,"abstract":"In this study, viability, growth performance and egg production performance were investigated to determine the productive characteristics of 12 Korean domestic chicken varieties which have been collected and conserved for over 25 years in National Institute of Animal Science, Rural Development Administration, Korea. The 1,134 hens were tested and their production traits including survival rate, body weight, age at first egg laying, hen-day and hen-housed egg production, egg weight, and egg quality were measured. Survival rate was the highest in Korean Rhode-D and Korean Native Chicken (KNC) White and the lowest in Korean Cornish Brown (92.2% and 54.3%, respectively). Body weights from 4 to 50 wks were consistently high in the order of Korean Cornish, Korean Rhode, KNC, Korean Ogye, and Korean Leghorn breeds. There was no significant difference in weight between varieties within a breed. Age at first egg laying was the shortest in Korean Leghorn, while it was the longest in Korean Cornish. The hen-day egg production from 1 egg to 57 wks was the highest in Korean Leghorn-K, and the lowest in Korean Cornish Brown. Egg weight was the heaviest in Korean Leghorn-F and the lightest in KNC White. The Haugh unit was the highest in Korean Rhode-C and the lowest in Korean Ogye. Taken together, these results suggest that it is desirable that the Korean Cornish variety is improved as the Korean meat breed because of its excellent growth ability, the Korean Leghorn variety is improved as the Korean layer breed because of its good laying performance, and the Korean Rhode and KNC varieties are improved as strong viable breeds because of their good survival rate. (","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75156467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
토종닭 농장주 및 가공업자가 바라본 토종닭 산업의 미래 방향 土鸡农场主及加工者眼中土鸡产业的未来方向
Korean Journal of Poultry Science Pub Date : 2019-03-01 DOI: 10.5536/kjps.2019.46.1.47
J. Yoon, Hyun Jung Lee, Ki-Chang Nam, Cheorun Jo
{"title":"토종닭 농장주 및 가공업자가 바라본 토종닭 산업의 미래 방향","authors":"J. Yoon, Hyun Jung Lee, Ki-Chang Nam, Cheorun Jo","doi":"10.5536/kjps.2019.46.1.47","DOIUrl":"https://doi.org/10.5536/kjps.2019.46.1.47","url":null,"abstract":"","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78366532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Dietary Bee Venom on Serum Characteristic, Antioxidant Activity and Liver Fatty Acid Composition in Broiler Chickens 饲粮中添加蜂毒对肉鸡血清特性、抗氧化活性和肝脏脂肪酸组成的影响
Korean Journal of Poultry Science Pub Date : 2019-03-01 DOI: 10.5536/KJPS.2019.46.1.39
Da-Hye Kim, S. Han, Yun-Sang Choi, H. Kang, Hong-Gu Lee, Kyung-Woo Lee
{"title":"Effects of Dietary Bee Venom on Serum Characteristic, Antioxidant\u0000 Activity and Liver Fatty Acid Composition in Broiler Chickens","authors":"Da-Hye Kim, S. Han, Yun-Sang Choi, H. Kang, Hong-Gu Lee, Kyung-Woo Lee","doi":"10.5536/KJPS.2019.46.1.39","DOIUrl":"https://doi.org/10.5536/KJPS.2019.46.1.39","url":null,"abstract":"This study was conducted to investigate the effect of dietary bee venom on serum characteristics, antioxidant activity, and hepatic fatty acid composition in broiler chickens. A group of 875 one-day-old feather-sexed male broiler chicks were randomly allocated to five treatments with seven replicates (25 birds/replicate) for three weeks. A corn-soybean meal-based diet was used as the basal diet. Five dietary treatments were compared: 1) basal diet, 2) basal diet containing 10 μg/kg of bee venom powder, 3) basal diet containing 50 μg/kg of bee venom powder, 4) basal diet containing 100 μg/kg of bee venom powder, and 5) basal diet containing 500 μg/kg of bee venom powder. At 21 days, one bird per pen was slaughtered by asphyxiation in CO2 gas, and blood was collected to measure serum characteristics and antioxidant activity. In addition, the liver was excised to measure the concentration of malondialdehyde and determine fatty acid composition. Increasing dietary bee venom in the diet failed to affect most serum parameters except for triglyceride and non-esterified fatty acids. Dietary bee venom inclusion quadratically increased the concentration of stearic acid (P<0.05), but decreased palmitoleic acid, oleic acid, linoleic acid, mono-unsaturated fatty acids, and poly-unsaturated fatty acids. Finally, dietary bee venom tended to lower hepatic malondialdehyde contents quadratically (P=0.054). In conclusion, our study revealed that dietary bee venom improved antioxidant capacity and affected fatty acid metabolism in broiler chickens. (","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81043873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Analysis of Transcriptional Activity and Estrogen Responsiveness of Regulatory Elements in Chicken Ovalbumin Promoter 鸡卵白蛋白启动子调控元件的转录活性及雌激素响应性分析
Korean Journal of Poultry Science Pub Date : 2019-03-01 DOI: 10.5536/KJPS.2019.46.1.17
Hyeon Yang, Kyungwoon Kim, J. Kim, J. Woo, Hwi-Cheul Lee, Hoonsung Choi, Sun Keun Jung, S. Sureshkumar, Haesun Lee, K. Oh, S. Byun
{"title":"Analysis of Transcriptional Activity and Estrogen Responsiveness of\u0000 Regulatory Elements in Chicken Ovalbumin Promoter","authors":"Hyeon Yang, Kyungwoon Kim, J. Kim, J. Woo, Hwi-Cheul Lee, Hoonsung Choi, Sun Keun Jung, S. Sureshkumar, Haesun Lee, K. Oh, S. Byun","doi":"10.5536/KJPS.2019.46.1.17","DOIUrl":"https://doi.org/10.5536/KJPS.2019.46.1.17","url":null,"abstract":"Chickens have been considered as well-defined animal bioreactor. The optimized ovalbumin promoter is essential for recombinant protein production in transgenic chicken. Here we try to compare the activity and identify the effect of estrogen on ovalbumin promoter according to each promoter length with estrogen response element (ERE) existence. We cloned two (2.8 and 5.5 kb) ovalbumin promoters that the 5.5 kb contained the ERE but the 2.8 kb did not, and these two promoters were cloned to pGL4.11 vector. Additionally, we constructed another pGL4.11 vector containing of the 4.4 kb (with ERE) ovalbumin promoter deleted with 1 kb between ERE region and the 2.8 kb promoter. For reporter assay, HeLa, MES-SA, LMH/2A, and cEF cells were transfected with all the pGL4.11 vectors. The comparative analysis showed that the mutated 4.4 kb promoter has more potent activity than the 2.8 and 5.5 kb promoters in HeLa, MES-SA, and LMH/2A cells. However, there is no significant difference in cEFs. Also, these cells transfected with the mutated 4.4 kb promoter were treated with the 17β -estradiol (0∼3,000 nM) and HeLa, MES-SA, and LMH/2A cells showed estrogen responsibilities, but cEFs did not. Besides, the mutated 4.4 kb promoter has still higher activity than the 2.8 and 5.5 kb promoter, and there is no transcriptional induction effect in 2.8 kb promoter at 500 nM estrogen that is blood concentration of laying hens. Hence our study strongly suggested that the mutated 4.4 kb promoter is considered as one of the most efficient length for generating transgenic chicken. (","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72862590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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