JURNAL SAINS NATURAL最新文献

{"title":"Utilization of Binahong (Anredera cordifolia) Leaves Extract from Bogor Regency as Free Radical Scavenging Activity","authors":"N. Utami, Usep Suhendar, Anisa Hidayatul Amini","doi":"10.31938/jsn.v12i1.327","DOIUrl":"https://doi.org/10.31938/jsn.v12i1.327","url":null,"abstract":"Binahong leaves contain flavonoid and polyphenol compounds that are potentially free radical scavengers. Extraction using ultrasonic has been carried out at various extraction times and temperatures. The purpose of this study was to obtain the optimum time and temperature of ultrasonic for yield and free radical scavenging activity of binahong leaves using Response Surface Methodology (RSM) with the Central Composite Design method. Binahong leaves were extracted at three levels of time (10, 20, 30 minutes) and temperature (40, 45, 50°C) with a frequency of 40 kHz using an ultrasonic bath. The results showed at a temperature of 45°C for 20 minutes obtained the highest yield of 10.44%, with a predictive value of RSM 10.15%. At a temperature of 40°C for 30 minutes, the optimum value of the free radical scavenging activity of binahong leaf extract was obtained. The percent inhibition value was 55.92%, with a predictive value of 48.85%. Based on the results, the temperature and time variables have a significant effect on the percentage of extract yield and have no significant effect on free radical scavenging activity.Keywords:  Binahong, Response surface methodology, Yield, free radical scavenging activityPemanfaatan Ekstrak Daun Binahong (Anredera cordifolia ) Asal Kabupaten Bogor Sebagai Penangkal Radikal BebasABSTRAKDaun binahong memiliki senyawa flavonoid dan polifenol yang berpotensi sebagai penangkal radikal bebas. Telah dilakukan ekstraksi dengan menggunakan bantuan gelombang ultrasonic yang dilakukan dengan berbagai waktu dan suhu ekstraksi. Tujuan dari penelitian ini yaitu diperolehnya waktu dan suhu optimum ekstraksi berbantu gelombang ultrasonik terhadap rendemen ekstrak dan aktivitas penangkal radikal bebas daun binahong dengan menggunakan Response Surface Methodology (RSM) metode Central Composite Design. Daun binahong diekstraksi dengan 3 taraf waktu (10, 20, 30 menit) dan suhu (40°C, 45°C, 50°C) dengan frekuensi 40 kHz dengan menggunakan ultrasonic bath. Hasil penelitian menunjukkan bahwa pada suhu 45°C pada waktu 20 menit diperoleh hasil rendemen ekstrak tertinggi yaitu 10,44% dengan nilai prediksi RSM 10,15% dan pada suhu 40°C dengan waktu 30 menit diperoleh nilai optimum terhadap aktivitas penangkal radikal bebas ekstrak daun binahong yaitu diperoleh nilai persen inhibisi 55,92% dengan nilai prediksi 48,85%. Berdasarkan hasil penelitian, menunjukkan bahwa variabel suhu dan waktu hanya berpengaruh nyata terhadap persentase rendemen ekstrak dan tidak berpengaruh nyata terhadap aktivitas penangkalan radikal bebas.Kata kunci: Binahong, Response surface methodology, Rendemen ekstrak, Aktivitas penangkal radikal bebas","PeriodicalId":17812,"journal":{"name":"JURNAL SAINS NATURAL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74979221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Molecular Identification of Endophytic Bacteria of Clove Leaf (Syzygium aromaticum L) and Mechanism of Action Antibacterial","authors":"Sogandi Sogandi, Irviyani Irviyani, Usep Suhendar","doi":"10.31938/jsn.v12i1.326","DOIUrl":"https://doi.org/10.31938/jsn.v12i1.326","url":null,"abstract":"Endophytic bacteria are microorganisms that live in plant parts. Endophytic bacteria from clove (Syzygium aromaticum L.) leaves with antibacterial activity are rarely reported. Therefore, this study aims to isolate endophytic bacteria from clove leaves (Syzygium aromaticum L.), screen and determine potential endophytic bacteria as antibacterial compounds, identify molecularly with 16S rRNA, analyze bacterial growth curves and detect bioactive compounds produced by potential bacteria. Endophytic bacteria were isolated as many as 7 pure isolates including IRV1, IRV2, IRV3, IRV4, IRV5, IRV6, IRV7. IRV3 isolates showed the most dominant activity in inhibiting the growth of test bacteria and as the most potential producer of antibacterial compounds. Molecular identification with PCR (polymerase chain reaction) amplification in the 16S rRNA gene showed IRV3 isolate as Staphylococcus sp. with a 99% similarity value. Besides, the results of GCMS analysis of secondary metabolites of IRV3 isolates showed pyrazine content as a dominant compound, which has been known as a bioactive compound in inhibiting bacterial growth. Therefore, the results of this study revealed that IRV3 isolates that were successfully isolated and identified would be able to contribute to further research to find new antibiotic sources.Keywords: Antibacterial, Endophytic, Clove Leaves, Bioactive, Syzygium aromaticumIsolasi dan Identifikasi Molekuler Bakteri Endofit Daun Cengkeh (Syzigium aromaticum L) dan Mekanisme Aksinya Sebagai AntibakteriABSTRAKBakteri endofit adalah mikroorganisme yang hidup di dalam bagian tanaman. Bakteri endofit dari daun cengkeh (Syzygium aromaticum L.) dengan aktivitas antibakteri masih jarang dilaporkan. Oleh karena itu, penelitian ini bertujuan untuk mengisolasi bakteri endofit dari daun cengkeh (Syzygium aromaticum L.), skrining dan menentukan bakteri endofit potensial sebagai penghasil senyawa antibakteri, mengidentifikasi secara molekuler dengan 16S rRNA, menganalisis kurva pertumbuhan bakteri dan mendeteksi senyawa bioaktif yang diproduksi oleh bakteri potensial. Bakteri endofit yang berhasil diisolasi sebanyak 7 isolat murni diantaranya IRV1, IRV2, IRV3, IRV4, IRV5, IRV6, IRV7. Isolat IRV3 menunjukkan aktivitas yang paling dominan dalam menghambat pertumbuhan bakteri uji dan sebagai penghasil senyawa antibakteri yang paling potensial. Identifikasi molekuler dengan amplifikasi PCR (polymerase chain reaction) pada gen 16S rRNA menunjukkan isolat IRV3 sebagai Staphylococcus sp. dengan nilai similaritas 99%. Selain itu, hasil analisis GCMS dari metabolit sekunder isolat IRV3 menunjukkan kandungan pirazin sebagai senyawa dominan, yang telah dikenal sebagai senyawa bioaktif dalam menghambat pertumbuhan bakteri. Oleh karena itu, hasil penelitian ini mengungkapkan bahwa isolate IRV3 yang berhasil diisolasi dan diidentifikasi akan dapat berkontribusi pada penelitian lebih lanjut untuk menemukan sumber antibiotik baru.Kata kunci: antibakteri, endofit, daun ceng","PeriodicalId":17812,"journal":{"name":"JURNAL SAINS NATURAL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74898016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of The Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) Method in Zinc Analysis","authors":"N. Bilqis, E. Sulistiawati, Miftakh Nur Rahman","doi":"10.31938/jsn.v12i1.321","DOIUrl":"https://doi.org/10.31938/jsn.v12i1.321","url":null,"abstract":"Zinc is a nutrient that affects cell growth, helps the body's metabolism, activates T cells (T lymphocytes), cell regeneration, and repairs body tissues. Zinc in the body undergoes a process of excretion every day, therefore daily intake of zinc needs to be considered to maintain the amount of zinc in the body in a normal amount. This study aimed to obtain information on the application of the Inductively coupled plasma-mass spectrometry (ICP-MS) method to determine the concentration of zinc in the serum of pregnant women. The application of Inductively coupled plasma-mass spectrometry (ICP-MS) method in zinc analysis provided the advantages of a minimal sample volume requirement with higher instrument sensitivity, fast retention time and had a concentration detection limit of up to parts per billion (ppb). Analysis of the zinc concentration in the serum of pregnant women using the ICP-MS method showed 80% serum with a concentration below the reference value, 13.3% normal and 6.7% a concentration higher than the WHO reference value.Keywords: defisiensi zink, inductively coupled plasma-mass spectrometry (icp-ms), zinkABSTRAKAplikasi Metode Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) Dalam Analisis ZinkZink adalah salah satu jenis zat gizi yang sangat berpengaruh terhadap pertumbuhan sel, mengaktifkan sel T (limfosit T), regenerasi sel, dan membantu metabolisme tubuh. Zink di dalam tubuh setiap harinya mengalami proses ekskresi oleh karena itu asupan harian zink perlu diperhatikan untuk menjaga jumlah zink di dalam tubuh tetap dalam jumlah yang normal. Penelitian ini bertujuan untuk mendapatkan informasi penggunaan metode Inductively coupled plasma-mass spectrometry (ICP-MS) untuk menentukan konsentrasi zink dalam serum ibu hamil. Penggunaan metode ICP-MS dalam analisis zink memberikan keuntungan berupa kebutuhan volume sampel minimal dengan sensitivitas alat lebih tinggi, waktu retensi yang cepat serta memiliki batas limit deteksi konsentrasi hingga bagian per triliun (ppt). Analisis konsentrasi zink dalam serum ibu hamil dengan metode ICP-MS menunjukan 80% serum dengan konsentrasi dibawah nilai rujukan, 13,3% normal dan 6,7% konsentrasi yang lebih tinggi dibandingkan nilai rujukan menurut WHO.Kata kunci : defisiensi zink, inductively coupled plasma-mass spectrometry (icp-ms), zink","PeriodicalId":17812,"journal":{"name":"JURNAL SAINS NATURAL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81490517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Nitrite and Nitrate Level in Wastewater Discharge from Smoked Fish Industry","authors":"Wina Yulianti, I. Kusumanti, N. Jannah","doi":"10.31938/jsn.v12i1.323","DOIUrl":"https://doi.org/10.31938/jsn.v12i1.323","url":null,"abstract":"This research aimed to determine nitrite and nitrate contamination in wastewater discharge from the smoked fish industry around the fishpond area in Penatarsewu village, Sidoarjo East Java. Samples were taken from ten locations along The Alo River from September to November 2020. Nitrate and nitrite levels were determined by the Standard Method for Examination of Water and Wastewater 4500-NO2-B dan 4500-NO3-B. Nitrite formed a reddishpurple azo dye produced by coupling diazotized sulfanilamide with N-(1-naphthyl)-ethylene-diamine dihydrochloride after a visible spectrophotometer measured the complexes nitrite at wavelength 543 nm. Meanwhile, the Nitrate level was determined by ultraviolet spectrophotometer at a wavelength of 220 nm. The standard nitrite calibration curve was shown by the line equation y= 3,30361x-0,0028 and a determination coefficient of 0.9999. Whereas the standard nitrate was showed by the line equation y=0,2507x + 0,0067 and a determination coefficient 0,9996. The level of nitrate and nitrite in the sample ranged from 0.05-0.93mg / L 1,2-1,6 mg / L. According to a quality standard threshold value for nitrite and nitrate-based on the Government Regulation of the Republic of Indonesia (PPRI) No.82 2001 is 0,06mg/L and 10 mg/L, therefore it can be concluded that some samples are above the threshold value for nitrite level.Keywords: Alo River,  nitate,  nitrite,  smoked fish,  spectrophotometerPenentuan Kadar Nitrit dan Nitrat Pembuangan Limbah Cair Industri Ikan AsapABSTRAKPenelitian bertujuan untuk menentukan tingkat kontaminasi nitrat dan nitrit pada limbah industri ikan asap di sekitar kawasan tambak ikan asap di Desa Penatarsewu, Sidoardjo, Jawa Timur. Sampel diambil dari 10 titik  di areal sekitar tambak sepanjang badan Sungai Alo pada bulan September sampai November 2020. Penentuan kadar nitrat dan nitrit mengacu pada Standard Methods for the Examination Water and Wastewater 4500-NO2-B dan 4500-NO3-B. Nitrit membentuk kompleks ungu kemerahan yang dihasilkan dari kopling sulfanilamida dengan N-(1-naptil)-etilena-diamina dihidroklorida kemudian kadarnya ditentukan dengan spektrofotometer sinar tampak pada panjang gelombang 543 nm. Kadar nitrat ditentukan menggunakan spektrofotometer ultraviolet pada panjang gelombang 220 nm. Hasil pengukuran standar nitrit menghasilkan persamaan garis y=3,30361x-0,0028 dengan koefesien determinasi 0,999 dan standar nitrat menghasilkan persamaan garis y=0,2507x+0,0067 dengan koefesien determinasi 0,9996. Kadar nitrit dan nitrat secara berturut-turut berkisar antara 0,05-0,93 mg/L dan 1,2-1,6 mg/L. Berdasarkan nilai ambang batas baku mutu nitrit dan nitrat berdasarkan Peraturan Pemerintah Republik Indonesia (PPRI) No.82 tahun 2001 adalah 0,06 mg/L dan 10 mg/L, maka dapat disimpulkan bahwa beberapa sampel mengandung kadar nitrit  di atas ambang batas.Kata kunci : ikan asap, nitrat, nitrit, Sungai Alo, spektrofotometer","PeriodicalId":17812,"journal":{"name":"JURNAL SAINS NATURAL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73927312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of Chlorine Tablets to Reducing Coliform in Wastewater Treatment Plant","authors":"Sri Arofah Mulyati, M. Azizah, Srikandi Srikandi, Maidaswar Maidaswar, Neneng Atikah","doi":"10.31938/jsn.v12i1.340","DOIUrl":"https://doi.org/10.31938/jsn.v12i1.340","url":null,"abstract":"Wastewater originating from experimental animal cages that are not treated properly can be a source of pollution because it contains organic, inorganic materials and even pathogenic microorganisms. Disinfection using chlorine is the last step in the wastewater treatment process to reduce microorganisms. This study aimed to determine the effectiveness of chlorine tablets in reducing the content of Coliform from experimental animal cage wastewater at the Wastewater Treatment Plant (WWTP) to meet the Total Coliform quality standard in the Regulation of the Minister of Environment and Forestry No. 68 of 2016 concerning domestic wastewater quality standards. The research was conducted using laboratory experiments pre and post-test. Wastewater samples were taken from 2 points, namely, the point inlet (before treatment) and outlet (after chlorination), the MPN test Coliform (SNI 2897 2008) were repeated twice, and the measured pH and temperature to analyze environmental factors. The results showed that 200 g of chlorine tablets could effectively reduce Coliform up to 98.55% and meet the quality standards used as a reference.Keywords: Coliform, Chlorine, Wastewater, WWTP, Quality standard Efektivitas Tablet Klorin Dalam Menurunkan Kandungan Coliform Pada Instalasi Pengolahan Air LimbahABSTRAKAir limbah yang berasumber dari kandang hewan coba yang tidak diolah dengan tepat dapat menjadi sumber pencemaran karena mengandung bahan organik, anorganik bahkan mikroorganisme pathogen. Desinfeksi meggunakan klorin merupakan tahapan terakhir pada proses pengolahan air limbah untuk mengurangi mikroorganisme. Tujuan dari penelitian ini adalah mengetahui efektivitas tablet klorin dalam menurunkan kandungan Coliform dari air limbah kandang hewan coba di Instalasi Pengolahan Air Limbah (IPAL) agar memenuhi baku mutu Total Coliform dalam Peraturan Menteri Lingkungan Hidup dan Kehutanan No. 68 Tahun 2016 tentang baku mutu air limbah domestik. Penelitian dilakukan dengan menggunakan eksperimen laoboratorium pre dan post test. Sampel air limbah diambil dari 2 titik yaitu titik inlet (sebelum pengolahan) dan outlet (sesudah klorinasi), uji MPN Coliform (SNI 2897 2008)  dilakukan dua kali ulangan, serta mengukur pH dan suhu untuk menganalisis faktor lingkungan. Hasil penelitian menunjukkan tablet klorin sebanyak 200 g efektif dapat menurunkan Coliform hingga 98,55 % dan memenuhi baku mutu yang dijadikan acuan.Kata Kunci: Coliform, Klorin, air limbah, IPAL dan baku mutu.","PeriodicalId":17812,"journal":{"name":"JURNAL SAINS NATURAL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87493307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Copper Dissolution from Electronic Waste Printed Circuit Board Using Leaching Method","authors":"Faradisa Athalla, E. Sulistiawati, I. Setiawan","doi":"10.31938/jsn.v12i1.322","DOIUrl":"https://doi.org/10.31938/jsn.v12i1.322","url":null,"abstract":"Electronic waste contains hazardous materials which have an adverse impact on the environment. Therefore, a solution is needed to recycle electronic waste. One solution is recovering copper metal from the Printed Circuit Board (PCB) in electronic waste. The dissolution of copper is carried out by the leaching method, which means dissolving copper using a selective solvent. Analysis of copper was performed using Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). This experiment aims to determine the factors that influence the optimization of copper dissolution from PCB electronic waste by leaching method and analysis the copper content using ICP-OES. The primary data obtained is the per cent recovery of each factor that affects copper dissolution, which is the concentration of sulfuric acid as a solvent, the percentage of solid / liquid, the speed of stirring, the temperature, and the length of stirring. The optimum conditions were achieved in the form of the highest recovery in each variation, which is 9.8% sulfuric acid, 5% solid/liquid percentage, 200 rpm stirring speed, and at temperature 60 °C with a stirring time of 120 minutes, the per cent recovery was obtained respectively 79.83%; 74.86%; 76.52%; and 54.84%.Keywords: electronic, waste,  PCB, dissolution, copper Optimalisasi Pelarutan Tembaga dari Limbah Elektronik Printed Circuit Board dengan Metode LeachingABSTRAKLimbah elektronik mengandung bahan-bahan berbahaya yang berdampak buruk bagi lingkungan. Oleh sebab itu, diperlukan solusi untuk mendaur ulang limbah elektronik salah satunya adalah pengambilan kembali atau recovery logam tembaga dari Printed Circuit Board (PCB) pada limbah elektronik. Pelarutan tembaga dilakukan dengan metode leaching, yaitu dengan melarutkan tembaga menggunakan pelarut yang selektif. Analisis terhadap kadar tembaga dilakukan menggunakan Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). Percobaan ini bertujuan untuk mendapatkan faktor-faktor yang berpengaruh pada optimalisasi pelarutan tembaga dari limbah elektronik PCB dengan metode leaching dengan analisis terhadap kadar tembaga menggunakan ICP-OES. Data primer yang diperoleh adalah persen recovery dari setiap faktor-faktor yang memengaruhi pelarutan tembaga, yaitu konsentrasi asam sulfat sebagai pelarut, persentase solid/liquid, kecepatan pengadukan, suhu, dan lama pengadukan. Kondisi optimum yang dicapai berupa recovery tertinggi pada setiap variasi, yaitu pada 9,8% asam sulfat, persentase 5% solid/liquid, kecepatan pengadukan 200 rpm, dan suhu pada 60 °C dengan lama pengadukan 120 menit diperoleh persen recovery secara berturut-turut 79,83%; 74,86%; 76,52%; dan 54,84%.Kata kunci : elektronik, limbah,  PCB, pelarut, tembaga","PeriodicalId":17812,"journal":{"name":"JURNAL SAINS NATURAL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84163435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inventory of butterfly species (Lepidoptera: Papilionoidea) in several habitat types in University of Bangka Belitung","authors":"Elfrida Natalia Manurung, B. Afriyansyah, H. Sutrisno","doi":"10.31938/jsn.v11i2.305","DOIUrl":"https://doi.org/10.31938/jsn.v11i2.305","url":null,"abstract":"Butterflies (Papilionoidea) are Lepidoptera which have various ecological roles in maintaining the balance of the ecosystem and can be found in various types of habitats ranging from forest to urban areas. This research was conducted from March to June 2020 in four types of habitat, namely, rubber gardens, secondary forest, agroecosystem hatchery, and swamp forest using the Pollard transect method and the sweeping net technique for five days in each habitat type. The relationships between environmental factors and the number of species and individuals were analyzed with the Pearson correlation test. The number of individual butterflies obtained was 913 butterflies of 27 species from five families (Nymphalidae, Pieridae, Lycaenidae, Hesperidae, and Riodinidae). The highest diversity was found in rubber gardens (19 species) followed by secondary forest (17 species), swamp forest (12 species) and agroecosystem hatchery (10 species). The highest species diversity index was found in swamp forests, while the lowest was in the agroecosystem hatchery habitat. The types of butterflies found in all habitats are Eurema sari, Mycalesis horsfieldi and M. mineus. The most abundant butterfly family is Nymphalidae. The Pearson correlation test analysis results showed a relationship between environmental factors and the number of species and individuals found.Keywords: Diversity, butterflies, Papilionoidea, habitat ABSTRAKInventarisasi jenis kupu-kupu (Lepidoptera: Papilionoidea) di beberapa tipe habitat di Universitas Bangka Belitung Kupu-kupu (Papilionoidea) merupakan Lepidoptera yang memiliki berbagai peran ekologis dalam menjaga keseimbangan ekosistem serta dapat ditemukan di berbagai macam tipe habitat mulai dari kawasan hutan hingga perkotaan. Penelitian ini dilakukan pada bulan Maret sampai Juni 2020 di empat tipe habitat yaitu, kebun karet, hutan sekunder, hatchery agroekosistem, dan hutan rawa dengan menggunakan metode transek Pollard dan teknik sweeping net selama lima hari di masing-masing tipe habitat. Hubungan faktor lingkungan dengan jumlah spesies dan jumlah individu dianalisis dengan uji korelasi Pearson. Jumlah individu kupu-kupu yang diperoleh sebanyak 913 ekor yang terdiri dari 27 spesies yang berasal dari lima famili (Nymphalidae, Pieridae, Lycaenidae, Hesperidae, dan Riodinidae). Keanekargaman tertinggi didapatkan di habitat kebun karet (19 spesies) selanjutnya diikuti hutan sekunder (17 spesies), hutan rawa (12 spesies) dan hatchery agroekosistem (10 spesies). Indeks keanekaragaman jenis paling tinggi ditemukan di hutan rawa, sedangkan yang paling rendah pada habitat hatchery agroekosistem. Jenis kupu-kupu yang ditemukan di seluruh habitat  yaitu Eurema sari, Mycalesis horsfieldi dan M. mineus. Famili kupu-kupu yang paling melimpah Nymphalidae. Hasil analisis uji kolerasi pearson, menunjukkan adanya hubungan antara faktor lingkungan dengan jumlah spesies dan individu yang ditemukan.Kata kunci: keanekaragaman, kupu-kupu, Papilionoidea, habita","PeriodicalId":17812,"journal":{"name":"JURNAL SAINS NATURAL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87661668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant activity from ethanol extract of red seaweed (Galaxaura rugosa)","authors":"Bhayu Gita Bhernama, Witri Maulidy Ayu, C. Nuzlia","doi":"10.31938/jsn.v11i2.309","DOIUrl":"https://doi.org/10.31938/jsn.v11i2.309","url":null,"abstract":"Antioxidants are compounds that can delay, reduce, slow down or inhibit oxidation reactions from free radical reactions. Antioxidants donate electrons to unstable free radicals so that these free radicals can be neutralized to not interfere with the body's metabolic processes. Red seaweed Galaxaura rugosa has potential as an antioxidant. The study aimed to determine the antioxidant activity of the ethanol extract of red seaweed Galaxaura rugosa against DPPH free radicals based on the IC50 value. Phytochemical testing and determination of antioxidant activity were carried out using the DPPH method (2,2-diphenyl-1-picrylhydrazyl), which was carried out quantitatively using a UV-Vis spectrophotometer. The results of the phytochemical screening of red seaweed ethanol extract contained alkaloids, flavonoids, saponins, steroids, and polyphenols and the IC50 value of red seaweed ethanol extract was 4.59 ppm, while the positive control for Vitamin C was 6.64 ppm. It was concluded that the ethanolic extract of red seaweed Galaxaura rugosa in the South Aceh District had high antioxidant potential, as evidenced by the small IC50 value of <50 µg/mL.Keywords: Antioxidants, Galauxara rugosa, phytochemicals, DPPH  ABSTRAKAktivitas antioksidan dari ekstrak etanol rumput laut merah Galaxaura rugosa Antioksidan merupakan senyawa yang mampu menunda, memperkecil, memperlambat atau menghambat reaksi oksidasi dari reaksi radikal bebas. Antioksidan menyumbangkan elektron kepada radikal bebas yang tidak stabil sehingga radikal bebas ini dapat dinetralkan agar tidak mengganggu jalannya proses metabolisme tubuh. Rumput laut merah Galaxaura rugosa berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan ekstrak etanol rumput laut merah Galaxaura rugosa terhadap radikal bebas DPPH berdasarkan nilai IC50. Pada penelitian ini dilakukan pengujian fitokimia dan penentuan aktivitas antioksidan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil) yang dilakukan secara kuantitatif menggunakan spektrofotometer UV-Vis. Hasil dari skrining fitokimia ekstrak etanol rumput laut merah mengandung senyawa alkaloid, flavonoid, saponin, steroid dan polifenol serta nilai IC50 ekstrak etanol rumput laut merah sebesar 4,59 ppm sedangkan kontrol positif Vitamin C sebesar 6,64 ppm. Ekstrak etanol rumput laut merah Galaxaura rugosa di Kabupaten Aceh Selatan memiliki potensi antioksidan yang tinggi. dibuktikan dengan nilai IC50 yang yang kecil yaitu < 50 µg/mL.Kata kunci: Antioksidan, Galauxara rugosa, Fitokimia, DPPH","PeriodicalId":17812,"journal":{"name":"JURNAL SAINS NATURAL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75046224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of herpetofauna and mammals on reclamation land PT. Refined Bangka Tin Bangka","authors":"R. S. Hasibuan, K. Cita, Suwandi Raharjo, Agus Seftian Pracahyo, Fari Indarto, Tanya Zurmie","doi":"10.31938/jsn.v11i2.296","DOIUrl":"https://doi.org/10.31938/jsn.v11i2.296","url":null,"abstract":"The success indicators of land reclamation can be seen in the presence of plants and animals. The more types of plants and animals that can live and reproduce, the better the condition of the land ecosystem. One of the indicators is the presence of herpetofauna and mammals, so this study aims to determine the diversity of wild animals consisting of herpetofauna, in this case, are reptiles and amphibians, and to determine the diversity of mammals in the area of PT. Refined Bangka Tin (PT. RBT). The research method was the Visual Encounter Survey (VES) for collecting herpetofauna data and the Path Transect method for collecting data on mammals. The results showed that seven species of reptiles were found with a diversity of 1.51, while there were three types of amphibians with a diversity of 1.04. The diversity of reptiles and amphibians based on the Shannon-Wiener diversity index is moderate. There are four species of mammals with a diversity of 1.12 based on the Shannon-Wiener diversity index value that the diversity of mammals is moderate.Keywords: herpetofauna, diversity, reclaimed land, mammalsKeanekaragaman herpetofauna dan mamalia pada lahan reklamasi  PT. Refined Bangka Tin, Bangka ABSTRAK Suatu perusahaan dikatakan berhasil mengelola lahan reklamasinya ditandai dengan adanya flora dan fauna. Semakin tinggi keanekaragaman flora dan fauna pada lahan reklamasi maka semakin baik keadaan ekosistem pada lahan reklamasi tersebut.    Salah satu fauna yang terdapat pada lahan reklamasi adalah herpetofauna dan mamalia, sehingga tujuan dari penelitian ini adalah untuk mengetahui keanekaragaman satwa liar yang terdiri dari herpetofauna dalam hal ini adalah reptil dan amfibi serta untuk mengetahui keanekaragaman mamalia di kawasan PT. Refined Bangka Tin (PT. RBT). Metode penelitian adalah Visual Encounter Survey (VES) untuk pengambilan data herpetofauna dan metode Transek Jalur untuk pengambilan data mamalia.  Hasil penelitian adalah satwa reptil ditemukan sebanyak 7 jenis dengan keanekaragaman 1,51, sedangkan amfibi ditemukan sebanyak 3 jenis dengan keanekaragaman sebesar 1,04.  Keanekaragaman reptil dan amfibi yang berdasar pada indeks keanekaragaman Shannon-Wiener adalah sedang.  Satwa mamalia ditemukan sebanyak 4 jenis dengan keanekaragaman sebesar 1,12 termasuk sedang berdasar nilai indeks keanekaragaman Shannon-Wiener.Kata kunci : herpetofauna, keanekaragaman, lahan reklamasi, mamalia","PeriodicalId":17812,"journal":{"name":"JURNAL SAINS NATURAL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90275188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opportunity and benefits of functional food from the sea: A Rewiew","authors":"Paulus Damar Bayu Murti, B. Dwiloka, O. Radjasa, J. Ngginak","doi":"10.31938/jsn.v11i2.297","DOIUrl":"https://doi.org/10.31938/jsn.v11i2.297","url":null,"abstract":"Functional food has been believed to prevent and reduce the possibility of chronic diseases such as obesity, diabetes, coronary heart disease, hypertension and cancer. The sea offers resources that can be used as a source of functional food. Research on bioactive compounds from marine life has been carried out, which have biological activity. For example, omega-3 consist of two types of acids, namely docosahexaenoic acid (DHA) and eicosapentanoic acid (EPA), which is contained in fish. Carotenoids and xanthophylls are abundant in macroalgae. Likewise with phenolic compounds and polysaccharides derived from algae. The protein hydrolysate from fish waste which is used as an alternative product has biological activity. Chitin and chitosan were extracted from crustacean shells and marine mollusk. Referring to the diversity of compound bioactivity from marine resources, this review emphasizes more on the potential of functional food ingredients owned by marine resources and their opportunities and benefits..Keywords: Functional food, Marine, Bioactive compounds, Chronic diseases, Biological activityABSTRAKPeluang dan manfaat pangan fungsional dari laut: Telaah PustakPangan fungsional telah dipercayai dapat mencegah dan menurunkan kemungkinan penyakit kronis seperti obesitas, diabetes, jantung koroner, hipertensi dan kanker. Laut menawarkan sumberdaya yang dapat dimanfaatkan sebagai sumber bahan pangan fungsional. Penelitian mengenai senyawa bioaktif dari biota laut sudah banyak dilakukan yang memiliki aktivitas biologis.  Sebagai contoh, Omega-3 terdiri atas dua jenis asam yaitu asam dokosaheksaenoat (DHA) dan asam eikosapentanoat (EPA) yang terkandung pada ikan. Karotenoid dan xantofil yang banyak terkandung pada makroalga. Begitu pun dengan senyawa fenolik dan polisakarida yang berasal dari alga. Hidrolisat protein dari limbah ikan yang dimanfaatkan sebagai produk alternatif memiliki aktivitas biologis. Kitin dan kitosan yang diekstrak dari limbah cangkang krustasea dan moluska laut. Mengacu pada keragaman bioaktivitas senyawa dari sumberdaya kelautan, review ini lebih menekankan pada potensi bahan pangan fungsional yang dimiliki oleh sumberdaya kelautan serta peluang dan manfaatnya.Kata kunci : Pangan fungsional, Laut, Senyawa bioaktif, Penyakit kronis, Aktivitas biiologi","PeriodicalId":17812,"journal":{"name":"JURNAL SAINS NATURAL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81273622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}